This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GATATT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 131.57 s (45 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 210,653 (7.1%) Reads that were too short: 13,205 (0.4%) Reads written (passing filters): 197,448 (6.7%) Total basepairs processed: 887,907,860 bp Total written (filtered): 55,439,160 bp (6.2%) === Adapter 1 === Sequence: GATATT; Type: regular 5'; Length: 6; Trimmed: 210653 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1286 720.2 0 1286 7 80769 720.2 0 80769 8 3190 720.2 0 3190 9 85477 720.2 0 85477 10 197 720.2 0 197 11 122 720.2 0 122 12 123 720.2 0 123 13 120 720.2 0 120 14 128 720.2 0 128 15 99 720.2 0 99 16 136 720.2 0 136 17 128 720.2 0 128 18 103 720.2 0 103 19 132 720.2 0 132 20 127 720.2 0 127 21 140 720.2 0 140 22 158 720.2 0 158 23 117 720.2 0 117 24 106 720.2 0 106 25 156 720.2 0 156 26 133 720.2 0 133 27 132 720.2 0 132 28 119 720.2 0 119 29 117 720.2 0 117 30 134 720.2 0 134 31 139 720.2 0 139 32 128 720.2 0 128 33 157 720.2 0 157 34 249 720.2 0 249 35 1332 720.2 0 1332 36 964 720.2 0 964 37 741 720.2 0 741 38 84 720.2 0 84 39 101 720.2 0 101 40 97 720.2 0 97 41 89 720.2 0 89 42 86 720.2 0 86 43 107 720.2 0 107 44 107 720.2 0 107 45 123 720.2 0 123 46 101 720.2 0 101 47 90 720.2 0 90 48 88 720.2 0 88 49 97 720.2 0 97 50 124 720.2 0 124 51 100 720.2 0 100 52 123 720.2 0 123 53 94 720.2 0 94 54 98 720.2 0 98 55 96 720.2 0 96 56 112 720.2 0 112 57 80 720.2 0 80 58 88 720.2 0 88 59 92 720.2 0 92 60 145 720.2 0 145 61 81 720.2 0 81 62 90 720.2 0 90 63 111 720.2 0 111 64 112 720.2 0 112 65 78 720.2 0 78 66 87 720.2 0 87 67 94 720.2 0 94 68 126 720.2 0 126 69 96 720.2 0 96 70 161 720.2 0 161 71 143 720.2 0 143 72 108 720.2 0 108 73 99 720.2 0 99 74 87 720.2 0 87 75 80 720.2 0 80 76 90 720.2 0 90 77 95 720.2 0 95 78 97 720.2 0 97 79 107 720.2 0 107 80 144 720.2 0 144 81 113 720.2 0 113 82 107 720.2 0 107 83 102 720.2 0 102 84 95 720.2 0 95 85 109 720.2 0 109 86 105 720.2 0 105 87 115 720.2 0 115 88 98 720.2 0 98 89 104 720.2 0 104 90 130 720.2 0 130 91 96 720.2 0 96 92 95 720.2 0 95 93 95 720.2 0 95 94 110 720.2 0 110 95 114 720.2 0 114 96 144 720.2 0 144 97 144 720.2 0 144 98 140 720.2 0 140 99 193 720.2 0 193 100 143 720.2 0 143 101 369 720.2 0 369 102 115 720.2 0 115 103 148 720.2 0 148 104 124 720.2 0 124 105 103 720.2 0 103 106 104 720.2 0 104 107 116 720.2 0 116 108 112 720.2 0 112 109 137 720.2 0 137 110 223 720.2 0 223 111 626 720.2 0 626 112 435 720.2 0 435 113 314 720.2 0 314 114 113 720.2 0 113 115 93 720.2 0 93 116 102 720.2 0 102 117 118 720.2 0 118 118 114 720.2 0 114 119 112 720.2 0 112 120 95 720.2 0 95 121 179 720.2 0 179 122 197 720.2 0 197 123 236 720.2 0 236 124 129 720.2 0 129 125 100 720.2 0 100 126 121 720.2 0 121 127 127 720.2 0 127 128 116 720.2 0 116 129 99 720.2 0 99 130 89 720.2 0 89 131 124 720.2 0 124 132 226 720.2 0 226 133 136 720.2 0 136 134 133 720.2 0 133 135 85 720.2 0 85 136 94 720.2 0 94 137 113 720.2 0 113 138 91 720.2 0 91 139 164 720.2 0 164 140 143 720.2 0 143 141 231 720.2 0 231 142 110 720.2 0 110 143 90 720.2 0 90 144 72 720.2 0 72 145 89 720.2 0 89 146 87 720.2 0 87 147 230 720.2 0 230 148 276 720.2 0 276 149 222 720.2 0 222 150 81 720.2 0 81 151 96 720.2 0 96 152 89 720.2 0 89 153 88 720.2 0 88 154 96 720.2 0 96 155 91 720.2 0 91 156 105 720.2 0 105 157 108 720.2 0 108 158 106 720.2 0 106 159 240 720.2 0 240 160 208 720.2 0 208 161 434 720.2 0 434 162 73 720.2 0 73 163 76 720.2 0 76 164 103 720.2 0 103 165 83 720.2 0 83 166 81 720.2 0 81 167 78 720.2 0 78 168 85 720.2 0 85 169 96 720.2 0 96 170 158 720.2 0 158 171 92 720.2 0 92 172 87 720.2 0 87 173 91 720.2 0 91 174 100 720.2 0 100 175 122 720.2 0 122 176 94 720.2 0 94 177 87 720.2 0 87 178 95 720.2 0 95 179 98 720.2 0 98 180 67 720.2 0 67 181 98 720.2 0 98 182 100 720.2 0 100 183 89 720.2 0 89 184 116 720.2 0 116 185 121 720.2 0 121 186 113 720.2 0 113 187 113 720.2 0 113 188 129 720.2 0 129 189 127 720.2 0 127 190 144 720.2 0 144 191 86 720.2 0 86 192 85 720.2 0 85 193 99 720.2 0 99 194 88 720.2 0 88 195 116 720.2 0 116 196 97 720.2 0 97 197 96 720.2 0 96 198 93 720.2 0 93 199 106 720.2 0 106 200 101 720.2 0 101 201 122 720.2 0 122 202 100 720.2 0 100 203 106 720.2 0 106 204 114 720.2 0 114 205 118 720.2 0 118 206 186 720.2 0 186 207 203 720.2 0 203 208 390 720.2 0 390 209 166 720.2 0 166 210 138 720.2 0 138 211 95 720.2 0 95 212 86 720.2 0 86 213 107 720.2 0 107 214 81 720.2 0 81 215 87 720.2 0 87 216 87 720.2 0 87 217 79 720.2 0 79 218 73 720.2 0 73 219 85 720.2 0 85 220 107 720.2 0 107 221 120 720.2 0 120 222 160 720.2 0 160 223 122 720.2 0 122 224 92 720.2 0 92 225 102 720.2 0 102 226 94 720.2 0 94 227 74 720.2 0 74 228 112 720.2 0 112 229 97 720.2 0 97 230 109 720.2 0 109 231 128 720.2 0 128 232 164 720.2 0 164 233 138 720.2 0 138 234 153 720.2 0 153 235 412 720.2 0 412 236 655 720.2 0 655 237 567 720.2 0 567 238 107 720.2 0 107 239 80 720.2 0 80 240 86 720.2 0 86 241 198 720.2 0 198 242 128 720.2 0 128 243 130 720.2 0 130 244 108 720.2 0 108 245 86 720.2 0 86 246 96 720.2 0 96 247 74 720.2 0 74 248 199 720.2 0 199 249 152 720.2 0 152 250 129 720.2 0 129 251 82 720.2 0 82 252 69 720.2 0 69 253 71 720.2 0 71 254 97 720.2 0 97 255 132 720.2 0 132 256 199 720.2 0 199 257 218 720.2 0 218 258 162 720.2 0 162 259 245 720.2 0 245 260 583 720.2 0 583 261 375 720.2 0 375 262 147 720.2 0 147 263 102 720.2 0 102 264 102 720.2 0 102 265 75 720.2 0 75 266 91 720.2 0 91 267 75 720.2 0 75 268 82 720.2 0 82 269 65 720.2 0 65 270 77 720.2 0 77 271 71 720.2 0 71 272 72 720.2 0 72 273 86 720.2 0 86 274 102 720.2 0 102 275 119 720.2 0 119 276 73 720.2 0 73 277 76 720.2 0 76 278 86 720.2 0 86 279 88 720.2 0 88 280 119 720.2 0 119 281 117 720.2 0 117 282 125 720.2 0 125 283 77 720.2 0 77 284 120 720.2 0 120 285 73 720.2 0 73 286 77 720.2 0 77 287 72 720.2 0 72 288 93 720.2 0 93 289 90 720.2 0 90 290 98 720.2 0 98 291 87 720.2 0 87 292 98 720.2 0 98 293 90 720.2 0 90 294 83 720.2 0 83 295 126 720.2 0 126 296 81 720.2 0 81 297 99 720.2 0 99 298 80 720.2 0 80 299 65 720.2 0 65 300 104 720.2 0 104 301 129 720.2 0 129 Finished in 13.52 s (68 us/read; 0.88 M reads/minute). === Summary === Total reads processed: 197,448 Reads with adapters: 89,194 (45.2%) Reads that were too short: 118 (0.1%) Reads written (passing filters): 89,076 (45.1%) Total basepairs processed: 55,439,160 bp Total written (filtered): 24,480,215 bp (44.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 89194 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 9 0.0 2 0 0 0 9 15 61 0.0 3 0 0 44 17 16 1038 0.0 3 0 945 57 36 17 87204 0.0 3 81947 4404 701 152 18 285 0.0 3 30 181 19 55 19 157 0.0 3 0 1 78 78 20 185 0.0 3 1 0 0 184 26 1 0.0 3 1 27 1 0.0 3 1 33 2 0.0 3 0 0 0 2 38 1 0.0 3 1 44 2 0.0 3 0 0 0 2 45 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 49 2 0.0 3 0 0 0 2 53 1 0.0 3 1 55 2 0.0 3 0 0 0 2 59 2 0.0 3 0 0 0 2 63 1 0.0 3 0 0 0 1 70 2 0.0 3 0 0 0 2 71 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 81 23 0.0 3 0 0 0 23 84 2 0.0 3 0 0 0 2 86 7 0.0 3 0 0 1 6 87 9 0.0 3 0 0 0 9 88 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 131 8 0.0 3 0 0 0 8 132 2 0.0 3 0 0 0 2 134 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 136 6 0.0 3 0 0 0 6 137 5 0.0 3 0 0 1 4 138 1 0.0 3 0 0 0 1 139 2 0.0 3 0 0 0 2 140 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 148 6 0.0 3 0 0 0 6 149 2 0.0 3 0 0 0 2 150 3 0.0 3 0 0 0 3 152 2 0.0 3 0 0 1 1 154 2 0.0 3 0 0 0 2 156 3 0.0 3 0 0 0 3 157 4 0.0 3 0 0 1 3 159 1 0.0 3 0 0 1 160 2 0.0 3 0 0 0 2 164 2 0.0 3 0 0 0 2 165 1 0.0 3 0 0 0 1 168 2 0.0 3 0 0 0 2 169 25 0.0 3 0 0 0 25 173 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 181 2 0.0 3 0 0 0 2 182 6 0.0 3 0 0 0 6 184 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 192 3 0.0 3 0 0 0 3 194 11 0.0 3 0 0 0 11 195 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 207 2 0.0 3 0 0 0 2 208 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 16 0.0 3 0 0 3 13 218 7 0.0 3 0 0 0 7 220 1 0.0 3 0 0 0 1 221 1 0.0 3 1 224 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 0 1 254 1 0.0 3 0 0 0 1 257 1 0.0 3 0 0 0 1 258 2 0.0 3 0 0 0 2 259 2 0.0 3 0 0 0 2 260 1 0.0 3 0 0 0 1 266 1 0.0 3 0 0 0 1 270 2 0.0 3 0 0 0 2 271 2 0.0 3 0 0 0 2 278 1 0.0 3 0 0 0 1 279 6 0.0 3 0 0 0 6 280 2 0.0 3 0 0 0 2 281 12 0.0 3 0 0 0 12 282 2 0.0 3 0 0 0 2 283 2 0.0 3 0 0 1 1 284 1 0.0 3 0 0 0 1 294 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.95 s (56 us/read; 1.08 M reads/minute). === Summary === Total reads processed: 89,076 Reads with adapters: 2,347 (2.6%) Reads written (passing filters): 89,076 (100.0%) Total basepairs processed: 24,480,215 bp Total written (filtered): 24,473,042 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 2347 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 54.7% C: 14.0% G: 29.4% T: 1.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2225 1391.8 0 2225 4 119 348.0 0 119 5 2 87.0 0 2 12 1 0.0 1 0 1