This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GATATT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 132.22 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 209,556 (7.1%) Reads that were too short: 11,088 (0.4%) Reads written (passing filters): 198,468 (6.7%) Total basepairs processed: 887,907,860 bp Total written (filtered): 56,113,086 bp (6.3%) === Adapter 1 === Sequence: GATATT; Type: regular 5'; Length: 6; Trimmed: 209556 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1228 720.2 0 1228 7 95977 720.2 0 95977 8 2191 720.2 0 2191 9 77493 720.2 0 77493 10 170 720.2 0 170 11 87 720.2 0 87 12 93 720.2 0 93 13 109 720.2 0 109 14 91 720.2 0 91 15 94 720.2 0 94 16 110 720.2 0 110 17 90 720.2 0 90 18 93 720.2 0 93 19 100 720.2 0 100 20 86 720.2 0 86 21 110 720.2 0 110 22 96 720.2 0 96 23 99 720.2 0 99 24 102 720.2 0 102 25 118 720.2 0 118 26 123 720.2 0 123 27 89 720.2 0 89 28 107 720.2 0 107 29 111 720.2 0 111 30 98 720.2 0 98 31 92 720.2 0 92 32 128 720.2 0 128 33 105 720.2 0 105 34 126 720.2 0 126 35 516 720.2 0 516 36 458 720.2 0 458 37 485 720.2 0 485 38 80 720.2 0 80 39 101 720.2 0 101 40 84 720.2 0 84 41 100 720.2 0 100 42 82 720.2 0 82 43 95 720.2 0 95 44 91 720.2 0 91 45 80 720.2 0 80 46 94 720.2 0 94 47 68 720.2 0 68 48 99 720.2 0 99 49 82 720.2 0 82 50 94 720.2 0 94 51 81 720.2 0 81 52 81 720.2 0 81 53 86 720.2 0 86 54 79 720.2 0 79 55 89 720.2 0 89 56 94 720.2 0 94 57 75 720.2 0 75 58 92 720.2 0 92 59 102 720.2 0 102 60 161 720.2 0 161 61 73 720.2 0 73 62 88 720.2 0 88 63 83 720.2 0 83 64 103 720.2 0 103 65 74 720.2 0 74 66 102 720.2 0 102 67 91 720.2 0 91 68 96 720.2 0 96 69 105 720.2 0 105 70 155 720.2 0 155 71 139 720.2 0 139 72 93 720.2 0 93 73 91 720.2 0 91 74 84 720.2 0 84 75 96 720.2 0 96 76 81 720.2 0 81 77 78 720.2 0 78 78 90 720.2 0 90 79 87 720.2 0 87 80 110 720.2 0 110 81 85 720.2 0 85 82 90 720.2 0 90 83 89 720.2 0 89 84 109 720.2 0 109 85 90 720.2 0 90 86 74 720.2 0 74 87 91 720.2 0 91 88 91 720.2 0 91 89 86 720.2 0 86 90 113 720.2 0 113 91 86 720.2 0 86 92 82 720.2 0 82 93 87 720.2 0 87 94 91 720.2 0 91 95 109 720.2 0 109 96 111 720.2 0 111 97 110 720.2 0 110 98 109 720.2 0 109 99 164 720.2 0 164 100 127 720.2 0 127 101 363 720.2 0 363 102 93 720.2 0 93 103 118 720.2 0 118 104 90 720.2 0 90 105 104 720.2 0 104 106 92 720.2 0 92 107 113 720.2 0 113 108 96 720.2 0 96 109 98 720.2 0 98 110 121 720.2 0 121 111 303 720.2 0 303 112 212 720.2 0 212 113 263 720.2 0 263 114 81 720.2 0 81 115 70 720.2 0 70 116 87 720.2 0 87 117 90 720.2 0 90 118 85 720.2 0 85 119 100 720.2 0 100 120 80 720.2 0 80 121 160 720.2 0 160 122 138 720.2 0 138 123 131 720.2 0 131 124 94 720.2 0 94 125 82 720.2 0 82 126 94 720.2 0 94 127 97 720.2 0 97 128 96 720.2 0 96 129 99 720.2 0 99 130 89 720.2 0 89 131 117 720.2 0 117 132 191 720.2 0 191 133 104 720.2 0 104 134 86 720.2 0 86 135 72 720.2 0 72 136 86 720.2 0 86 137 89 720.2 0 89 138 96 720.2 0 96 139 110 720.2 0 110 140 122 720.2 0 122 141 182 720.2 0 182 142 88 720.2 0 88 143 87 720.2 0 87 144 83 720.2 0 83 145 99 720.2 0 99 146 104 720.2 0 104 147 143 720.2 0 143 148 160 720.2 0 160 149 138 720.2 0 138 150 64 720.2 0 64 151 78 720.2 0 78 152 92 720.2 0 92 153 100 720.2 0 100 154 69 720.2 0 69 155 84 720.2 0 84 156 105 720.2 0 105 157 108 720.2 0 108 158 95 720.2 0 95 159 314 720.2 0 314 160 229 720.2 0 229 161 584 720.2 0 584 162 74 720.2 0 74 163 68 720.2 0 68 164 87 720.2 0 87 165 91 720.2 0 91 166 67 720.2 0 67 167 115 720.2 0 115 168 80 720.2 0 80 169 87 720.2 0 87 170 125 720.2 0 125 171 88 720.2 0 88 172 103 720.2 0 103 173 79 720.2 0 79 174 100 720.2 0 100 175 88 720.2 0 88 176 74 720.2 0 74 177 72 720.2 0 72 178 71 720.2 0 71 179 74 720.2 0 74 180 80 720.2 0 80 181 76 720.2 0 76 182 109 720.2 0 109 183 89 720.2 0 89 184 96 720.2 0 96 185 92 720.2 0 92 186 108 720.2 0 108 187 104 720.2 0 104 188 106 720.2 0 106 189 94 720.2 0 94 190 132 720.2 0 132 191 102 720.2 0 102 192 96 720.2 0 96 193 82 720.2 0 82 194 108 720.2 0 108 195 133 720.2 0 133 196 89 720.2 0 89 197 70 720.2 0 70 198 96 720.2 0 96 199 75 720.2 0 75 200 87 720.2 0 87 201 105 720.2 0 105 202 88 720.2 0 88 203 103 720.2 0 103 204 95 720.2 0 95 205 104 720.2 0 104 206 165 720.2 0 165 207 184 720.2 0 184 208 398 720.2 0 398 209 116 720.2 0 116 210 105 720.2 0 105 211 83 720.2 0 83 212 79 720.2 0 79 213 80 720.2 0 80 214 103 720.2 0 103 215 78 720.2 0 78 216 74 720.2 0 74 217 69 720.2 0 69 218 77 720.2 0 77 219 76 720.2 0 76 220 86 720.2 0 86 221 100 720.2 0 100 222 126 720.2 0 126 223 84 720.2 0 84 224 105 720.2 0 105 225 94 720.2 0 94 226 78 720.2 0 78 227 72 720.2 0 72 228 81 720.2 0 81 229 86 720.2 0 86 230 87 720.2 0 87 231 96 720.2 0 96 232 112 720.2 0 112 233 102 720.2 0 102 234 107 720.2 0 107 235 355 720.2 0 355 236 592 720.2 0 592 237 464 720.2 0 464 238 89 720.2 0 89 239 88 720.2 0 88 240 104 720.2 0 104 241 222 720.2 0 222 242 138 720.2 0 138 243 122 720.2 0 122 244 108 720.2 0 108 245 62 720.2 0 62 246 62 720.2 0 62 247 68 720.2 0 68 248 193 720.2 0 193 249 133 720.2 0 133 250 120 720.2 0 120 251 63 720.2 0 63 252 49 720.2 0 49 253 54 720.2 0 54 254 69 720.2 0 69 255 92 720.2 0 92 256 175 720.2 0 175 257 204 720.2 0 204 258 92 720.2 0 92 259 202 720.2 0 202 260 444 720.2 0 444 261 197 720.2 0 197 262 112 720.2 0 112 263 79 720.2 0 79 264 68 720.2 0 68 265 70 720.2 0 70 266 56 720.2 0 56 267 75 720.2 0 75 268 64 720.2 0 64 269 60 720.2 0 60 270 63 720.2 0 63 271 78 720.2 0 78 272 80 720.2 0 80 273 91 720.2 0 91 274 88 720.2 0 88 275 99 720.2 0 99 276 62 720.2 0 62 277 59 720.2 0 59 278 55 720.2 0 55 279 83 720.2 0 83 280 88 720.2 0 88 281 83 720.2 0 83 282 88 720.2 0 88 283 81 720.2 0 81 284 83 720.2 0 83 285 78 720.2 0 78 286 82 720.2 0 82 287 72 720.2 0 72 288 69 720.2 0 69 289 58 720.2 0 58 290 70 720.2 0 70 291 74 720.2 0 74 292 62 720.2 0 62 293 85 720.2 0 85 294 82 720.2 0 82 295 82 720.2 0 82 296 68 720.2 0 68 297 81 720.2 0 81 298 65 720.2 0 65 299 80 720.2 0 80 300 59 720.2 0 59 301 102 720.2 0 102 Finished in 14.80 s (75 us/read; 0.80 M reads/minute). === Summary === Total reads processed: 198,468 Reads with adapters: 98,887 (49.8%) Reads that were too short: 25 (0.0%) Reads written (passing filters): 98,862 (49.8%) Total basepairs processed: 56,113,086 bp Total written (filtered): 26,808,248 bp (47.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 98887 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 15 0.0 3 0 0 13 2 19 848 0.0 3 0 821 23 2 2 20 95083 0.0 4 93368 1642 63 7 3 21 262 0.0 4 56 205 1 22 17 0.0 4 1 0 15 1 23 61 0.0 4 1 0 2 49 9 24 2 0.0 4 0 0 0 1 1 30 1 0.0 4 1 35 1 0.0 4 1 38 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 99 21 0.0 4 0 0 0 0 21 104 4 0.0 4 0 0 0 0 4 111 1 0.0 4 0 1 114 1 0.0 4 1 120 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 5 0.0 4 0 0 0 0 5 126 20 0.0 4 0 0 0 0 20 127 2504 0.0 4 0 0 0 0 2504 128 7 0.0 4 0 0 0 0 7 129 2 0.0 4 0 0 0 0 2 130 2 0.0 4 0 0 0 0 2 131 1 0.0 4 1 136 1 0.0 4 0 0 0 0 1 137 3 0.0 4 1 2 161 1 0.0 4 1 165 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 0 1 198 1 0.0 4 0 0 0 0 1 201 1 0.0 4 0 0 0 0 1 211 1 0.0 4 1 215 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 228 5 0.0 4 0 0 0 0 5 229 1 0.0 4 0 0 0 1 230 2 0.0 4 0 0 0 0 2 234 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 5.16 s (52 us/read; 1.15 M reads/minute). === Summary === Total reads processed: 98,862 Reads with adapters: 1,158 (1.2%) Reads written (passing filters): 98,862 (100.0%) Total basepairs processed: 26,808,248 bp Total written (filtered): 26,793,880 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1158 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 15.0% C: 6.0% G: 76.3% T: 2.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 195 1544.7 0 195 4 20 386.2 0 20 5 48 96.5 0 48 6 1 24.1 0 1 9 8 0.4 0 4 4 10 27 0.1 1 2 25 11 635 0.0 1 8 627 12 198 0.0 1 4 194 13 4 0.0 1 0 4 15 2 0.0 1 0 2 152 8 0.0 1 0 8 171 2 0.0 1 2 179 1 0.0 1 0 1 210 6 0.0 1 5 1 225 3 0.0 1 3