This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAAGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 139.71 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 694,786 (23.6%) Reads that were too short: 10,317 (0.3%) Reads written (passing filters): 684,469 (23.2%) Total basepairs processed: 887,907,860 bp Total written (filtered): 191,632,687 bp (21.6%) === Adapter 1 === Sequence: GAAGCT; Type: regular 5'; Length: 6; Trimmed: 694786 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4475 720.2 0 4475 7 14252 720.2 0 14252 8 316116 720.2 0 316116 9 265413 720.2 0 265413 10 339 720.2 0 339 11 59 720.2 0 59 12 54 720.2 0 54 13 28 720.2 0 28 14 28 720.2 0 28 15 25 720.2 0 25 16 34 720.2 0 34 17 31 720.2 0 31 18 36 720.2 0 36 19 27 720.2 0 27 20 58 720.2 0 58 21 52 720.2 0 52 22 36 720.2 0 36 23 33 720.2 0 33 24 44 720.2 0 44 25 46 720.2 0 46 26 55 720.2 0 55 27 34 720.2 0 34 28 45 720.2 0 45 29 30 720.2 0 30 30 71 720.2 0 71 31 40 720.2 0 40 32 55 720.2 0 55 33 43 720.2 0 43 34 112 720.2 0 112 35 261 720.2 0 261 36 69 720.2 0 69 37 88 720.2 0 88 38 50 720.2 0 50 39 59 720.2 0 59 40 31 720.2 0 31 41 37 720.2 0 37 42 42 720.2 0 42 43 49 720.2 0 49 44 33 720.2 0 33 45 36 720.2 0 36 46 58 720.2 0 58 47 174 720.2 0 174 48 56 720.2 0 56 49 35 720.2 0 35 50 40 720.2 0 40 51 59 720.2 0 59 52 61 720.2 0 61 53 55 720.2 0 55 54 137 720.2 0 137 55 116 720.2 0 116 56 42 720.2 0 42 57 42 720.2 0 42 58 27 720.2 0 27 59 28 720.2 0 28 60 47 720.2 0 47 61 61 720.2 0 61 62 38 720.2 0 38 63 44 720.2 0 44 64 101 720.2 0 101 65 32 720.2 0 32 66 62 720.2 0 62 67 65 720.2 0 65 68 61 720.2 0 61 69 109 720.2 0 109 70 57 720.2 0 57 71 64 720.2 0 64 72 64 720.2 0 64 73 73 720.2 0 73 74 61 720.2 0 61 75 75 720.2 0 75 76 104 720.2 0 104 77 167 720.2 0 167 78 179 720.2 0 179 79 405 720.2 0 405 80 566 720.2 0 566 81 10830 720.2 0 10830 82 4978 720.2 0 4978 83 9537 720.2 0 9537 84 103 720.2 0 103 85 226 720.2 0 226 86 449 720.2 0 449 87 517 720.2 0 517 88 56 720.2 0 56 89 40 720.2 0 40 90 38 720.2 0 38 91 24 720.2 0 24 92 70 720.2 0 70 93 68 720.2 0 68 94 31 720.2 0 31 95 42 720.2 0 42 96 69 720.2 0 69 97 47 720.2 0 47 98 96 720.2 0 96 99 51 720.2 0 51 100 46 720.2 0 46 101 60 720.2 0 60 102 178 720.2 0 178 103 122 720.2 0 122 104 147 720.2 0 147 105 82 720.2 0 82 106 161 720.2 0 161 107 132 720.2 0 132 108 201 720.2 0 201 109 386 720.2 0 386 110 104 720.2 0 104 111 148 720.2 0 148 112 230 720.2 0 230 113 112 720.2 0 112 114 118 720.2 0 118 115 153 720.2 0 153 116 124 720.2 0 124 117 201 720.2 0 201 118 255 720.2 0 255 119 482 720.2 0 482 120 519 720.2 0 519 121 685 720.2 0 685 122 13077 720.2 0 13077 123 6641 720.2 0 6641 124 14549 720.2 0 14549 125 119 720.2 0 119 126 67 720.2 0 67 127 49 720.2 0 49 128 86 720.2 0 86 129 69 720.2 0 69 130 47 720.2 0 47 131 54 720.2 0 54 132 39 720.2 0 39 133 46 720.2 0 46 134 51 720.2 0 51 135 66 720.2 0 66 136 44 720.2 0 44 137 43 720.2 0 43 138 57 720.2 0 57 139 69 720.2 0 69 140 62 720.2 0 62 141 71 720.2 0 71 142 596 720.2 0 596 143 708 720.2 0 708 144 1065 720.2 0 1065 145 242 720.2 0 242 146 364 720.2 0 364 147 156 720.2 0 156 148 90 720.2 0 90 149 151 720.2 0 151 150 117 720.2 0 117 151 208 720.2 0 208 152 85 720.2 0 85 153 86 720.2 0 86 154 114 720.2 0 114 155 82 720.2 0 82 156 42 720.2 0 42 157 29 720.2 0 29 158 68 720.2 0 68 159 82 720.2 0 82 160 76 720.2 0 76 161 31 720.2 0 31 162 35 720.2 0 35 163 39 720.2 0 39 164 45 720.2 0 45 165 44 720.2 0 44 166 162 720.2 0 162 167 64 720.2 0 64 168 44 720.2 0 44 169 88 720.2 0 88 170 56 720.2 0 56 171 121 720.2 0 121 172 40 720.2 0 40 173 56 720.2 0 56 174 37 720.2 0 37 175 54 720.2 0 54 176 39 720.2 0 39 177 44 720.2 0 44 178 71 720.2 0 71 179 47 720.2 0 47 180 115 720.2 0 115 181 156 720.2 0 156 182 2109 720.2 0 2109 183 1081 720.2 0 1081 184 1495 720.2 0 1495 185 66 720.2 0 66 186 61 720.2 0 61 187 51 720.2 0 51 188 27 720.2 0 27 189 44 720.2 0 44 190 45 720.2 0 45 191 22 720.2 0 22 192 39 720.2 0 39 193 55 720.2 0 55 194 126 720.2 0 126 195 79 720.2 0 79 196 102 720.2 0 102 197 31 720.2 0 31 198 93 720.2 0 93 199 63 720.2 0 63 200 75 720.2 0 75 201 41 720.2 0 41 202 32 720.2 0 32 203 29 720.2 0 29 204 22 720.2 0 22 205 37 720.2 0 37 206 47 720.2 0 47 207 57 720.2 0 57 208 30 720.2 0 30 209 32 720.2 0 32 210 34 720.2 0 34 211 43 720.2 0 43 212 43 720.2 0 43 213 45 720.2 0 45 214 30 720.2 0 30 215 36 720.2 0 36 216 20 720.2 0 20 217 17 720.2 0 17 218 34 720.2 0 34 219 54 720.2 0 54 220 61 720.2 0 61 221 40 720.2 0 40 222 38 720.2 0 38 223 32 720.2 0 32 224 27 720.2 0 27 225 32 720.2 0 32 226 41 720.2 0 41 227 72 720.2 0 72 228 68 720.2 0 68 229 60 720.2 0 60 230 49 720.2 0 49 231 57 720.2 0 57 232 36 720.2 0 36 233 32 720.2 0 32 234 36 720.2 0 36 235 33 720.2 0 33 236 46 720.2 0 46 237 42 720.2 0 42 238 44 720.2 0 44 239 51 720.2 0 51 240 266 720.2 0 266 241 113 720.2 0 113 242 129 720.2 0 129 243 38 720.2 0 38 244 50 720.2 0 50 245 54 720.2 0 54 246 55 720.2 0 55 247 52 720.2 0 52 248 30 720.2 0 30 249 41 720.2 0 41 250 113 720.2 0 113 251 28 720.2 0 28 252 67 720.2 0 67 253 66 720.2 0 66 254 58 720.2 0 58 255 69 720.2 0 69 256 55 720.2 0 55 257 47 720.2 0 47 258 58 720.2 0 58 259 56 720.2 0 56 260 52 720.2 0 52 261 88 720.2 0 88 262 72 720.2 0 72 263 150 720.2 0 150 264 126 720.2 0 126 265 69 720.2 0 69 266 761 720.2 0 761 267 478 720.2 0 478 268 879 720.2 0 879 269 156 720.2 0 156 270 129 720.2 0 129 271 54 720.2 0 54 272 43 720.2 0 43 273 45 720.2 0 45 274 354 720.2 0 354 275 236 720.2 0 236 276 284 720.2 0 284 277 99 720.2 0 99 278 56 720.2 0 56 279 115 720.2 0 115 280 105 720.2 0 105 281 141 720.2 0 141 282 85 720.2 0 85 283 97 720.2 0 97 284 33 720.2 0 33 285 46 720.2 0 46 286 97 720.2 0 97 287 179 720.2 0 179 288 383 720.2 0 383 289 222 720.2 0 222 290 183 720.2 0 183 291 75 720.2 0 75 292 126 720.2 0 126 293 118 720.2 0 118 294 311 720.2 0 311 295 135 720.2 0 135 296 281 720.2 0 281 297 108 720.2 0 108 298 112 720.2 0 112 299 77 720.2 0 77 300 85 720.2 0 85 301 88 720.2 0 88 Finished in 45.19 s (66 us/read; 0.91 M reads/minute). === Summary === Total reads processed: 684,469 Reads with adapters: 321,531 (47.0%) Reads that were too short: 619 (0.1%) Reads written (passing filters): 320,912 (46.9%) Total basepairs processed: 191,632,687 bp Total written (filtered): 88,434,218 bp (46.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 321531 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 37 0.0 2 0 0 0 37 15 154 0.0 3 0 0 115 39 16 1955 0.0 3 0 1697 171 87 17 315765 0.0 3 301853 11889 1644 379 18 902 0.0 3 29 747 61 65 19 516 0.0 3 4 2 317 193 20 601 0.0 3 11 1 3 586 21 2 0.0 3 0 0 0 2 22 22 0.0 3 0 0 0 22 23 2 0.0 3 2 24 3 0.0 3 1 0 0 2 25 14 0.0 3 0 0 7 7 26 1 0.0 3 1 27 1 0.0 3 0 0 0 1 28 15 0.0 3 0 0 0 15 29 2 0.0 3 0 0 0 2 31 1 0.0 3 1 32 1 0.0 3 0 0 0 1 34 146 0.0 3 0 0 0 146 37 1 0.0 3 0 0 0 1 38 4 0.0 3 1 0 0 3 44 4 0.0 3 1 0 0 3 45 1 0.0 3 0 0 0 1 46 2 0.0 3 0 0 0 2 47 4 0.0 3 0 0 0 4 48 3 0.0 3 0 0 0 3 49 58 0.0 3 0 0 0 58 50 462 0.0 3 0 0 6 456 51 11 0.0 3 0 0 0 11 52 12 0.0 3 0 0 1 11 53 51 0.0 3 0 0 1 50 54 5 0.0 3 0 0 0 5 55 2 0.0 3 0 0 0 2 56 1 0.0 3 0 0 0 1 60 3 0.0 3 0 0 0 3 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 63 4 0.0 3 0 0 0 4 64 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 68 2 0.0 3 0 0 0 2 69 4 0.0 3 0 0 0 4 70 3 0.0 3 0 0 0 3 71 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 73 8 0.0 3 0 0 0 8 74 4 0.0 3 0 0 0 4 75 3 0.0 3 0 0 0 3 76 22 0.0 3 0 0 0 22 77 2 0.0 3 0 0 0 2 78 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 1 83 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 90 2 0.0 3 0 0 0 2 91 1 0.0 3 0 0 0 1 93 8 0.0 3 0 0 0 8 95 1 0.0 3 0 0 0 1 100 3 0.0 3 0 0 1 2 101 17 0.0 3 0 0 0 17 102 5 0.0 3 0 0 0 5 105 1 0.0 3 0 0 1 106 21 0.0 3 0 0 0 21 107 4 0.0 3 0 0 0 4 109 6 0.0 3 0 0 0 6 110 4 0.0 3 0 0 0 4 113 1 0.0 3 0 0 0 1 114 3 0.0 3 0 0 0 3 115 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 118 3 0.0 3 0 0 0 3 121 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 138 5 0.0 3 0 0 0 5 139 1 0.0 3 0 0 0 1 140 2 0.0 3 0 0 0 2 141 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 145 1 0.0 3 1 146 1 0.0 3 0 0 0 1 147 2 0.0 3 0 0 0 2 150 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 154 2 0.0 3 0 0 0 2 156 5 0.0 3 0 0 0 5 157 13 0.0 3 0 0 0 13 158 5 0.0 3 0 0 0 5 159 3 0.0 3 0 0 0 3 160 3 0.0 3 0 0 1 2 161 8 0.0 3 0 0 1 7 162 82 0.0 3 0 0 4 78 163 22 0.0 3 0 0 0 22 164 19 0.0 3 0 0 1 18 165 95 0.0 3 0 0 2 93 166 12 0.0 3 0 0 0 12 167 3 0.0 3 0 0 0 3 169 4 0.0 3 0 0 0 4 170 1 0.0 3 0 0 0 1 172 1 0.0 3 1 179 2 0.0 3 0 0 1 1 180 1 0.0 3 0 0 0 1 183 3 0.0 3 0 0 0 3 186 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 190 2 0.0 3 0 0 0 2 191 1 0.0 3 0 0 0 1 192 7 0.0 3 0 0 0 7 194 32 0.0 3 0 0 2 30 195 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 205 10 0.0 3 0 0 1 9 206 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 216 4 0.0 3 0 0 0 4 217 89 0.0 3 0 0 6 83 218 6 0.0 3 0 0 0 6 219 1 0.0 3 0 0 0 1 221 7 0.0 3 0 0 0 7 222 2 0.0 3 0 0 0 2 225 2 0.0 3 0 0 0 2 233 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 237 2 0.0 3 0 0 0 2 238 2 0.0 3 0 0 0 2 239 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 251 2 0.0 3 1 0 0 1 264 1 0.0 3 0 0 0 1 267 1 0.0 3 0 0 0 1 270 10 0.0 3 0 0 0 10 271 1 0.0 3 0 0 0 1 272 1 0.0 3 0 0 0 1 274 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 15 0.0 3 0 0 0 15 280 28 0.0 3 0 0 0 28 281 27 0.0 3 0 0 0 27 282 17 0.0 3 0 0 0 17 283 5 0.0 3 0 0 1 4 284 1 0.0 3 0 0 0 1 288 7 0.0 3 0 0 0 7 289 3 0.0 3 0 0 0 3 291 3 0.0 3 0 0 0 3 292 3 0.0 3 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 17.11 s (53 us/read; 1.13 M reads/minute). === Summary === Total reads processed: 320,912 Reads with adapters: 1,656 (0.5%) Reads written (passing filters): 320,912 (100.0%) Total basepairs processed: 88,434,218 bp Total written (filtered): 88,428,841 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1656 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 54.0% C: 11.0% G: 28.5% T: 6.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1302 5014.2 0 1302 4 308 1253.6 0 308 5 41 313.4 0 41 6 3 78.3 0 3 7 1 19.6 0 1 9 1 1.2 0 0 1