This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCCGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 58.32 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 470,291 (21.0%) Reads that were too short: 302,213 (13.5%) Reads written (passing filters): 168,078 (7.5%) Total basepairs processed: 673,916,425 bp Total written (filtered): 43,914,233 bp (6.5%) === Adapter 1 === Sequence: GTCCGG; Type: regular 5'; Length: 6; Trimmed: 470291 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 6713 546.6 0 6713 7 64916 546.6 0 64916 8 1389 546.6 0 1389 9 61020 546.6 0 61020 10 100 546.6 0 100 11 68 546.6 0 68 12 32 546.6 0 32 13 38 546.6 0 38 14 12 546.6 0 12 15 18 546.6 0 18 16 27 546.6 0 27 17 18 546.6 0 18 18 39 546.6 0 39 19 27 546.6 0 27 20 24 546.6 0 24 21 23 546.6 0 23 22 23 546.6 0 23 23 17 546.6 0 17 24 15 546.6 0 15 25 17 546.6 0 17 26 22 546.6 0 22 27 16 546.6 0 16 28 9 546.6 0 9 29 29 546.6 0 29 30 25 546.6 0 25 31 58 546.6 0 58 32 94 546.6 0 94 33 38 546.6 0 38 34 67 546.6 0 67 35 79 546.6 0 79 36 23 546.6 0 23 37 30 546.6 0 30 38 25 546.6 0 25 39 37 546.6 0 37 40 20 546.6 0 20 41 39 546.6 0 39 42 25 546.6 0 25 43 40 546.6 0 40 44 27 546.6 0 27 45 28 546.6 0 28 46 20 546.6 0 20 47 27 546.6 0 27 48 24 546.6 0 24 49 24 546.6 0 24 50 30 546.6 0 30 51 17 546.6 0 17 52 24 546.6 0 24 53 35 546.6 0 35 54 36 546.6 0 36 55 31 546.6 0 31 56 21 546.6 0 21 57 38 546.6 0 38 58 21 546.6 0 21 59 32 546.6 0 32 60 308 546.6 0 308 61 22 546.6 0 22 62 20 546.6 0 20 63 31 546.6 0 31 64 19 546.6 0 19 65 48 546.6 0 48 66 22 546.6 0 22 67 24 546.6 0 24 68 21 546.6 0 21 69 20 546.6 0 20 70 24 546.6 0 24 71 28 546.6 0 28 72 28 546.6 0 28 73 40 546.6 0 40 74 20 546.6 0 20 75 49 546.6 0 49 76 36 546.6 0 36 77 90 546.6 0 90 78 33 546.6 0 33 79 25 546.6 0 25 80 19 546.6 0 19 81 23 546.6 0 23 82 20 546.6 0 20 83 17 546.6 0 17 84 20 546.6 0 20 85 27 546.6 0 27 86 28 546.6 0 28 87 20 546.6 0 20 88 22 546.6 0 22 89 33 546.6 0 33 90 26 546.6 0 26 91 29 546.6 0 29 92 37 546.6 0 37 93 28 546.6 0 28 94 30 546.6 0 30 95 34 546.6 0 34 96 28 546.6 0 28 97 33 546.6 0 33 98 23 546.6 0 23 99 27 546.6 0 27 100 46 546.6 0 46 101 38 546.6 0 38 102 38 546.6 0 38 103 60 546.6 0 60 104 41 546.6 0 41 105 32 546.6 0 32 106 47 546.6 0 47 107 37 546.6 0 37 108 36 546.6 0 36 109 31 546.6 0 31 110 36 546.6 0 36 111 41 546.6 0 41 112 64 546.6 0 64 113 455 546.6 0 455 114 810 546.6 0 810 115 568 546.6 0 568 116 46 546.6 0 46 117 30 546.6 0 30 118 32 546.6 0 32 119 28 546.6 0 28 120 36 546.6 0 36 121 39 546.6 0 39 122 53 546.6 0 53 123 50 546.6 0 50 124 40 546.6 0 40 125 50 546.6 0 50 126 44 546.6 0 44 127 37 546.6 0 37 128 47 546.6 0 47 129 101 546.6 0 101 130 46 546.6 0 46 131 24 546.6 0 24 132 50 546.6 0 50 133 61 546.6 0 61 134 87 546.6 0 87 135 68 546.6 0 68 136 71 546.6 0 71 137 82 546.6 0 82 138 57 546.6 0 57 139 43 546.6 0 43 140 64 546.6 0 64 141 57 546.6 0 57 142 58 546.6 0 58 143 83 546.6 0 83 144 88 546.6 0 88 145 103 546.6 0 103 146 89 546.6 0 89 147 903 546.6 0 903 148 1512 546.6 0 1512 149 2521 546.6 0 2521 150 384 546.6 0 384 151 34 546.6 0 34 152 46 546.6 0 46 153 45 546.6 0 45 154 48 546.6 0 48 155 54 546.6 0 54 156 80 546.6 0 80 157 75 546.6 0 75 158 54 546.6 0 54 159 69 546.6 0 69 160 65 546.6 0 65 161 89 546.6 0 89 162 62 546.6 0 62 163 57 546.6 0 57 164 47 546.6 0 47 165 60 546.6 0 60 166 43 546.6 0 43 167 33 546.6 0 33 168 27 546.6 0 27 169 149 546.6 0 149 170 143 546.6 0 143 171 29 546.6 0 29 172 41 546.6 0 41 173 57 546.6 0 57 174 66 546.6 0 66 175 60 546.6 0 60 176 51 546.6 0 51 177 42 546.6 0 42 178 67 546.6 0 67 179 106 546.6 0 106 180 62 546.6 0 62 181 129 546.6 0 129 182 143 546.6 0 143 183 128 546.6 0 128 184 102 546.6 0 102 185 135 546.6 0 135 186 184 546.6 0 184 187 233 546.6 0 233 188 211 546.6 0 211 189 1127 546.6 0 1127 190 563 546.6 0 563 191 2489 546.6 0 2489 192 806 546.6 0 806 193 511 546.6 0 511 194 444 546.6 0 444 195 624 546.6 0 624 196 537 546.6 0 537 197 415 546.6 0 415 198 616 546.6 0 616 199 1485 546.6 0 1485 200 3356 546.6 0 3356 201 5436 546.6 0 5436 202 8047 546.6 0 8047 203 1573 546.6 0 1573 204 1356 546.6 0 1356 205 17186 546.6 0 17186 206 22827 546.6 0 22827 207 47587 546.6 0 47587 208 19005 546.6 0 19005 209 17191 546.6 0 17191 210 13070 546.6 0 13070 211 2239 546.6 0 2239 212 961 546.6 0 961 213 714 546.6 0 714 214 606 546.6 0 606 215 1268 546.6 0 1268 216 748 546.6 0 748 217 552 546.6 0 552 218 1237 546.6 0 1237 219 1614 546.6 0 1614 220 2663 546.6 0 2663 221 2172 546.6 0 2172 222 1989 546.6 0 1989 223 1630 546.6 0 1630 224 2310 546.6 0 2310 225 6486 546.6 0 6486 226 21782 546.6 0 21782 227 43629 546.6 0 43629 228 24656 546.6 0 24656 229 86 546.6 0 86 230 33 546.6 0 33 231 19 546.6 0 19 232 26 546.6 0 26 233 18 546.6 0 18 234 28 546.6 0 28 235 18 546.6 0 18 236 29 546.6 0 29 237 28 546.6 0 28 238 39 546.6 0 39 239 15 546.6 0 15 240 22 546.6 0 22 241 30 546.6 0 30 242 35 546.6 0 35 243 34 546.6 0 34 244 94 546.6 0 94 245 131 546.6 0 131 246 169 546.6 0 169 247 67 546.6 0 67 248 149 546.6 0 149 249 201 546.6 0 201 250 105 546.6 0 105 251 122 546.6 0 122 252 160 546.6 0 160 253 151 546.6 0 151 254 151 546.6 0 151 255 246 546.6 0 246 256 146 546.6 0 146 257 210 546.6 0 210 258 236 546.6 0 236 259 572 546.6 0 572 260 540 546.6 0 540 261 710 546.6 0 710 262 6587 546.6 0 6587 263 8239 546.6 0 8239 264 15653 546.6 0 15653 265 132 546.6 0 132 266 43 546.6 0 43 267 12 546.6 0 12 268 27 546.6 0 27 269 43 546.6 0 43 270 48 546.6 0 48 271 93 546.6 0 93 272 47 546.6 0 47 273 29 546.6 0 29 274 110 546.6 0 110 275 92 546.6 0 92 276 53 546.6 0 53 277 28 546.6 0 28 278 32 546.6 0 32 279 25 546.6 0 25 280 15 546.6 0 15 281 14 546.6 0 14 282 35 546.6 0 35 283 108 546.6 0 108 284 82 546.6 0 82 285 35 546.6 0 35 286 23 546.6 0 23 287 22 546.6 0 22 288 15 546.6 0 15 289 24 546.6 0 24 290 15 546.6 0 15 291 20 546.6 0 20 292 101 546.6 0 101 293 122 546.6 0 122 294 273 546.6 0 273 295 55 546.6 0 55 296 59 546.6 0 59 297 60 546.6 0 60 298 24 546.6 0 24 299 25 546.6 0 25 300 33 546.6 0 33 301 42 546.6 0 42 Finished in 7.89 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 168,078 Reads with adapters: 70,968 (42.2%) Reads that were too short: 34 (0.0%) Reads written (passing filters): 70,934 (42.2%) Total basepairs processed: 43,914,233 bp Total written (filtered): 19,399,502 bp (44.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 70968 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 16 0.0 3 0 0 16 19 792 0.0 3 0 775 16 1 20 69525 0.0 4 68329 1154 32 6 4 21 159 0.0 4 39 119 1 22 19 0.0 4 12 0 7 23 35 0.0 4 0 0 0 29 6 24 4 0.0 4 0 0 0 0 4 26 3 0.0 4 3 36 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 1 91 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 105 2 0.0 4 0 0 0 0 2 106 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 8 0.0 4 0 0 0 0 8 127 351 0.0 4 0 0 0 0 351 128 2 0.0 4 0 0 0 0 2 135 14 0.0 4 0 0 0 0 14 136 2 0.0 4 0 0 0 0 2 140 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 192 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 227 2 0.0 4 1 1 228 4 0.0 4 0 0 0 0 4 229 5 0.0 4 0 0 0 0 5 232 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 244 1 0.0 4 0 0 0 0 1 249 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 264 2 0.0 4 0 0 0 0 2 270 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.43 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 70,934 Reads with adapters: 858 (1.2%) Reads written (passing filters): 70,934 (100.0%) Total basepairs processed: 19,399,502 bp Total written (filtered): 19,389,715 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 858 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 15.2% C: 7.1% G: 75.6% T: 2.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 157 1108.3 0 157 4 11 277.1 0 11 5 3 69.3 0 3 6 2 17.3 0 2 8 1 1.1 0 1 9 8 0.3 0 0 8 10 17 0.1 1 0 17 11 398 0.0 1 9 389 12 217 0.0 1 61 156 13 30 0.0 1 15 15 14 3 0.0 1 2 1 15 1 0.0 1 0 1 124 1 0.0 1 0 1 140 1 0.0 1 1 152 5 0.0 1 0 5 153 1 0.0 1 0 1 179 1 0.0 1 1 210 1 0.0 1 0 1