This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 59.63 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 434,872 (19.4%) Reads that were too short: 20,635 (0.9%) Reads written (passing filters): 414,237 (18.5%) Total basepairs processed: 673,916,425 bp Total written (filtered): 117,296,305 bp (17.4%) === Adapter 1 === Sequence: TCCGCT; Type: regular 5'; Length: 6; Trimmed: 434872 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3047 546.6 0 3047 7 14988 546.6 0 14988 8 190246 546.6 0 190246 9 174092 546.6 0 174092 10 228 546.6 0 228 11 29 546.6 0 29 12 34 546.6 0 34 13 14 546.6 0 14 14 23 546.6 0 23 15 12 546.6 0 12 16 13 546.6 0 13 17 29 546.6 0 29 18 23 546.6 0 23 19 12 546.6 0 12 20 24 546.6 0 24 21 15 546.6 0 15 22 23 546.6 0 23 23 23 546.6 0 23 24 25 546.6 0 25 25 37 546.6 0 37 26 7 546.6 0 7 27 38 546.6 0 38 28 13 546.6 0 13 29 26 546.6 0 26 30 22 546.6 0 22 31 18 546.6 0 18 32 47 546.6 0 47 33 52 546.6 0 52 34 142 546.6 0 142 35 46 546.6 0 46 36 51 546.6 0 51 37 222 546.6 0 222 38 76 546.6 0 76 39 98 546.6 0 98 40 53 546.6 0 53 41 37 546.6 0 37 42 322 546.6 0 322 43 11 546.6 0 11 44 26 546.6 0 26 45 19 546.6 0 19 46 30 546.6 0 30 47 23 546.6 0 23 48 29 546.6 0 29 49 42 546.6 0 42 50 19 546.6 0 19 51 29 546.6 0 29 52 467 546.6 0 467 53 107 546.6 0 107 54 94 546.6 0 94 55 16 546.6 0 16 56 18 546.6 0 18 57 22 546.6 0 22 58 19 546.6 0 19 59 19 546.6 0 19 60 41 546.6 0 41 61 44 546.6 0 44 62 39 546.6 0 39 63 26 546.6 0 26 64 12 546.6 0 12 65 33 546.6 0 33 66 63 546.6 0 63 67 30 546.6 0 30 68 52 546.6 0 52 69 18 546.6 0 18 70 22 546.6 0 22 71 43 546.6 0 43 72 38 546.6 0 38 73 68 546.6 0 68 74 12 546.6 0 12 75 21 546.6 0 21 76 21 546.6 0 21 77 20 546.6 0 20 78 20 546.6 0 20 79 17 546.6 0 17 80 23 546.6 0 23 81 45 546.6 0 45 82 54 546.6 0 54 83 44 546.6 0 44 84 297 546.6 0 297 85 65 546.6 0 65 86 86 546.6 0 86 87 22 546.6 0 22 88 25 546.6 0 25 89 21 546.6 0 21 90 95 546.6 0 95 91 45 546.6 0 45 92 73 546.6 0 73 93 117 546.6 0 117 94 65 546.6 0 65 95 107 546.6 0 107 96 28 546.6 0 28 97 142 546.6 0 142 98 15 546.6 0 15 99 16 546.6 0 16 100 20 546.6 0 20 101 14 546.6 0 14 102 11 546.6 0 11 103 29 546.6 0 29 104 64 546.6 0 64 105 62 546.6 0 62 106 74 546.6 0 74 107 134 546.6 0 134 108 811 546.6 0 811 109 84 546.6 0 84 110 160 546.6 0 160 111 63 546.6 0 63 112 209 546.6 0 209 113 116 546.6 0 116 114 34 546.6 0 34 115 23 546.6 0 23 116 31 546.6 0 31 117 88 546.6 0 88 118 50 546.6 0 50 119 53 546.6 0 53 120 21 546.6 0 21 121 21 546.6 0 21 122 14 546.6 0 14 123 18 546.6 0 18 124 44 546.6 0 44 125 49 546.6 0 49 126 76 546.6 0 76 127 450 546.6 0 450 128 86 546.6 0 86 129 148 546.6 0 148 130 46 546.6 0 46 131 45 546.6 0 45 132 92 546.6 0 92 133 46 546.6 0 46 134 33 546.6 0 33 135 27 546.6 0 27 136 39 546.6 0 39 137 58 546.6 0 58 138 78 546.6 0 78 139 102 546.6 0 102 140 217 546.6 0 217 141 296 546.6 0 296 142 483 546.6 0 483 143 1167 546.6 0 1167 144 12790 546.6 0 12790 145 2305 546.6 0 2305 146 2008 546.6 0 2008 147 31 546.6 0 31 148 27 546.6 0 27 149 62 546.6 0 62 150 36 546.6 0 36 151 348 546.6 0 348 152 240 546.6 0 240 153 202 546.6 0 202 154 333 546.6 0 333 155 107 546.6 0 107 156 220 546.6 0 220 157 93 546.6 0 93 158 123 546.6 0 123 159 107 546.6 0 107 160 28 546.6 0 28 161 34 546.6 0 34 162 16 546.6 0 16 163 27 546.6 0 27 164 21 546.6 0 21 165 38 546.6 0 38 166 25 546.6 0 25 167 26 546.6 0 26 168 18 546.6 0 18 169 19 546.6 0 19 170 33 546.6 0 33 171 53 546.6 0 53 172 72 546.6 0 72 173 50 546.6 0 50 174 33 546.6 0 33 175 33 546.6 0 33 176 20 546.6 0 20 177 57 546.6 0 57 178 40 546.6 0 40 179 278 546.6 0 278 180 117 546.6 0 117 181 79 546.6 0 79 182 18 546.6 0 18 183 21 546.6 0 21 184 29 546.6 0 29 185 42 546.6 0 42 186 45 546.6 0 45 187 29 546.6 0 29 188 35 546.6 0 35 189 29 546.6 0 29 190 185 546.6 0 185 191 27 546.6 0 27 192 109 546.6 0 109 193 31 546.6 0 31 194 30 546.6 0 30 195 27 546.6 0 27 196 48 546.6 0 48 197 35 546.6 0 35 198 157 546.6 0 157 199 88 546.6 0 88 200 52 546.6 0 52 201 43 546.6 0 43 202 27 546.6 0 27 203 30 546.6 0 30 204 28 546.6 0 28 205 59 546.6 0 59 206 52 546.6 0 52 207 31 546.6 0 31 208 28 546.6 0 28 209 23 546.6 0 23 210 15 546.6 0 15 211 25 546.6 0 25 212 29 546.6 0 29 213 26 546.6 0 26 214 32 546.6 0 32 215 14 546.6 0 14 216 28 546.6 0 28 217 32 546.6 0 32 218 32 546.6 0 32 219 52 546.6 0 52 220 26 546.6 0 26 221 29 546.6 0 29 222 17 546.6 0 17 223 28 546.6 0 28 224 25 546.6 0 25 225 78 546.6 0 78 226 52 546.6 0 52 227 43 546.6 0 43 228 75 546.6 0 75 229 83 546.6 0 83 230 126 546.6 0 126 231 63 546.6 0 63 232 59 546.6 0 59 233 67 546.6 0 67 234 67 546.6 0 67 235 382 546.6 0 382 236 676 546.6 0 676 237 306 546.6 0 306 238 199 546.6 0 199 239 136 546.6 0 136 240 377 546.6 0 377 241 123 546.6 0 123 242 114 546.6 0 114 243 40 546.6 0 40 244 42 546.6 0 42 245 71 546.6 0 71 246 88 546.6 0 88 247 222 546.6 0 222 248 113 546.6 0 113 249 198 546.6 0 198 250 160 546.6 0 160 251 131 546.6 0 131 252 329 546.6 0 329 253 89 546.6 0 89 254 47 546.6 0 47 255 59 546.6 0 59 256 68 546.6 0 68 257 69 546.6 0 69 258 153 546.6 0 153 259 347 546.6 0 347 260 3956 546.6 0 3956 261 1997 546.6 0 1997 262 2111 546.6 0 2111 263 263 546.6 0 263 264 40 546.6 0 40 265 53 546.6 0 53 266 110 546.6 0 110 267 118 546.6 0 118 268 136 546.6 0 136 269 78 546.6 0 78 270 250 546.6 0 250 271 124 546.6 0 124 272 467 546.6 0 467 273 54 546.6 0 54 274 35 546.6 0 35 275 35 546.6 0 35 276 46 546.6 0 46 277 235 546.6 0 235 278 68 546.6 0 68 279 142 546.6 0 142 280 241 546.6 0 241 281 253 546.6 0 253 282 151 546.6 0 151 283 113 546.6 0 113 284 111 546.6 0 111 285 306 546.6 0 306 286 101 546.6 0 101 287 98 546.6 0 98 288 295 546.6 0 295 289 150 546.6 0 150 290 212 546.6 0 212 291 448 546.6 0 448 292 386 546.6 0 386 293 406 546.6 0 406 294 418 546.6 0 418 295 302 546.6 0 302 296 163 546.6 0 163 297 49 546.6 0 49 298 37 546.6 0 37 299 46 546.6 0 46 300 52 546.6 0 52 301 39 546.6 0 39 Finished in 17.40 s (42 us/read; 1.43 M reads/minute). === Summary === Total reads processed: 414,237 Reads with adapters: 200,118 (48.3%) Reads that were too short: 242 (0.1%) Reads written (passing filters): 199,876 (48.3%) Total basepairs processed: 117,296,305 bp Total written (filtered): 55,166,578 bp (47.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 200118 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 34 0.0 2 0 0 0 34 15 101 0.0 3 0 0 59 42 16 1605 0.0 3 0 1307 181 117 17 196203 0.0 3 183392 10426 1883 502 18 481 0.0 3 17 358 57 49 19 580 0.0 3 1 1 327 251 20 773 0.0 3 0 0 0 773 21 1 0.0 3 1 22 4 0.0 3 2 0 0 2 23 1 0.0 3 1 24 2 0.0 3 2 26 2 0.0 3 2 29 1 0.0 3 1 30 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 33 2 0.0 3 1 0 0 1 38 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 47 2 0.0 3 0 0 0 2 48 4 0.0 3 0 0 0 4 50 1 0.0 3 0 0 0 1 53 3 0.0 3 0 0 0 3 57 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 60 2 0.0 3 0 0 0 2 61 1 0.0 3 0 0 0 1 62 3 0.0 3 1 0 0 2 64 2 0.0 3 0 0 0 2 66 1 0.0 3 0 0 1 67 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 84 3 0.0 3 0 0 0 3 88 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 98 2 0.0 3 0 0 0 2 99 3 0.0 3 0 0 0 3 103 1 0.0 3 0 0 0 1 109 2 0.0 3 0 0 0 2 114 2 0.0 3 0 0 0 2 115 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 126 2 0.0 3 0 0 0 2 128 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 1 142 2 0.0 3 0 0 0 2 144 2 0.0 3 0 0 0 2 145 2 0.0 3 0 0 0 2 147 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 151 12 0.0 3 0 0 0 12 154 1 0.0 3 0 0 0 1 156 2 0.0 3 0 0 0 2 159 1 0.0 3 0 0 0 1 160 2 0.0 3 0 0 0 2 163 1 0.0 3 0 0 0 1 164 3 0.0 3 0 0 0 3 165 3 0.0 3 0 0 0 3 166 3 0.0 3 0 0 0 3 168 1 0.0 3 0 0 0 1 175 2 0.0 3 0 0 0 2 177 2 0.0 3 0 0 0 2 179 2 0.0 3 0 0 0 2 182 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 184 7 0.0 3 0 0 0 7 187 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 8 0.0 3 0 0 0 8 193 2 0.0 3 0 0 0 2 194 25 0.0 3 0 1 0 24 195 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 205 6 0.0 3 0 0 0 6 206 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 216 2 0.0 3 0 0 0 2 217 39 0.0 3 0 0 4 35 218 2 0.0 3 0 0 0 2 221 4 0.0 3 0 0 0 4 222 7 0.0 3 0 0 0 7 226 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 228 1 0.0 3 0 0 0 1 232 1 0.0 3 0 0 0 1 235 2 0.0 3 0 0 0 2 236 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 242 1 0.0 3 0 0 0 1 252 1 0.0 3 0 0 0 1 260 1 0.0 3 0 0 0 1 264 1 0.0 3 0 0 0 1 269 1 0.0 3 0 0 0 1 270 7 0.0 3 0 0 0 7 271 4 0.0 3 0 0 0 4 272 2 0.0 3 0 0 0 2 274 1 0.0 3 0 0 0 1 275 1 0.0 3 0 0 1 278 6 0.0 3 0 0 0 6 279 13 0.0 3 0 0 0 13 280 19 0.0 3 0 0 0 19 281 20 0.0 3 0 0 0 20 282 12 0.0 3 0 0 0 12 283 5 0.0 3 0 0 1 4 285 3 0.0 3 0 0 0 3 286 1 0.0 3 0 0 0 1 287 2 0.0 3 0 0 0 2 288 2 0.0 3 0 0 1 1 290 1 0.0 3 0 0 0 1 291 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 6.88 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 199,876 Reads with adapters: 1,307 (0.7%) Reads written (passing filters): 199,876 (100.0%) Total basepairs processed: 55,166,578 bp Total written (filtered): 55,162,452 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1307 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 45.0% C: 18.5% G: 28.5% T: 8.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1166 3123.1 0 1166 4 99 780.8 0 99 5 33 195.2 0 33 6 5 48.8 0 5 7 1 12.2 0 1 10 3 0.2 1 0 3