This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 59.12 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 228,700 (10.2%) Reads that were too short: 12,760 (0.6%) Reads written (passing filters): 215,940 (9.6%) Total basepairs processed: 673,916,425 bp Total written (filtered): 61,584,431 bp (9.1%) === Adapter 1 === Sequence: ACAGGT; Type: regular 5'; Length: 6; Trimmed: 228700 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2134 546.6 0 2134 7 115129 546.6 0 115129 8 81329 546.6 0 81329 9 98 546.6 0 98 10 32 546.6 0 32 11 24 546.6 0 24 12 27 546.6 0 27 13 22 546.6 0 22 14 18 546.6 0 18 15 25 546.6 0 25 16 24 546.6 0 24 17 28 546.6 0 28 18 114 546.6 0 114 19 29 546.6 0 29 20 88 546.6 0 88 21 32 546.6 0 32 22 36 546.6 0 36 23 18 546.6 0 18 24 21 546.6 0 21 25 33 546.6 0 33 26 39 546.6 0 39 27 45 546.6 0 45 28 23 546.6 0 23 29 22 546.6 0 22 30 33 546.6 0 33 31 24 546.6 0 24 32 25 546.6 0 25 33 31 546.6 0 31 34 36 546.6 0 36 35 30 546.6 0 30 36 28 546.6 0 28 37 25 546.6 0 25 38 37 546.6 0 37 39 29 546.6 0 29 40 23 546.6 0 23 41 36 546.6 0 36 42 18 546.6 0 18 43 28 546.6 0 28 44 35 546.6 0 35 45 34 546.6 0 34 46 30 546.6 0 30 47 28 546.6 0 28 48 30 546.6 0 30 49 24 546.6 0 24 50 27 546.6 0 27 51 22 546.6 0 22 52 35 546.6 0 35 53 31 546.6 0 31 54 46 546.6 0 46 55 37 546.6 0 37 56 29 546.6 0 29 57 34 546.6 0 34 58 34 546.6 0 34 59 49 546.6 0 49 60 26 546.6 0 26 61 46 546.6 0 46 62 24 546.6 0 24 63 28 546.6 0 28 64 28 546.6 0 28 65 39 546.6 0 39 66 52 546.6 0 52 67 61 546.6 0 61 68 60 546.6 0 60 69 118 546.6 0 118 70 190 546.6 0 190 71 2111 546.6 0 2111 72 433 546.6 0 433 73 1075 546.6 0 1075 74 38 546.6 0 38 75 40 546.6 0 40 76 34 546.6 0 34 77 65 546.6 0 65 78 43 546.6 0 43 79 172 546.6 0 172 80 44 546.6 0 44 81 52 546.6 0 52 82 42 546.6 0 42 83 36 546.6 0 36 84 45 546.6 0 45 85 70 546.6 0 70 86 44 546.6 0 44 87 85 546.6 0 85 88 44 546.6 0 44 89 17 546.6 0 17 90 48 546.6 0 48 91 38 546.6 0 38 92 29 546.6 0 29 93 43 546.6 0 43 94 97 546.6 0 97 95 158 546.6 0 158 96 710 546.6 0 710 97 373 546.6 0 373 98 550 546.6 0 550 99 57 546.6 0 57 100 26 546.6 0 26 101 25 546.6 0 25 102 22 546.6 0 22 103 30 546.6 0 30 104 39 546.6 0 39 105 36 546.6 0 36 106 27 546.6 0 27 107 28 546.6 0 28 108 39 546.6 0 39 109 32 546.6 0 32 110 26 546.6 0 26 111 30 546.6 0 30 112 36 546.6 0 36 113 32 546.6 0 32 114 43 546.6 0 43 115 45 546.6 0 45 116 50 546.6 0 50 117 53 546.6 0 53 118 74 546.6 0 74 119 43 546.6 0 43 120 30 546.6 0 30 121 29 546.6 0 29 122 50 546.6 0 50 123 54 546.6 0 54 124 144 546.6 0 144 125 905 546.6 0 905 126 320 546.6 0 320 127 70 546.6 0 70 128 94 546.6 0 94 129 93 546.6 0 93 130 106 546.6 0 106 131 46 546.6 0 46 132 75 546.6 0 75 133 64 546.6 0 64 134 60 546.6 0 60 135 91 546.6 0 91 136 48 546.6 0 48 137 54 546.6 0 54 138 50 546.6 0 50 139 50 546.6 0 50 140 75 546.6 0 75 141 76 546.6 0 76 142 53 546.6 0 53 143 62 546.6 0 62 144 55 546.6 0 55 145 50 546.6 0 50 146 54 546.6 0 54 147 37 546.6 0 37 148 42 546.6 0 42 149 56 546.6 0 56 150 47 546.6 0 47 151 46 546.6 0 46 152 40 546.6 0 40 153 43 546.6 0 43 154 45 546.6 0 45 155 71 546.6 0 71 156 55 546.6 0 55 157 41 546.6 0 41 158 42 546.6 0 42 159 44 546.6 0 44 160 37 546.6 0 37 161 38 546.6 0 38 162 32 546.6 0 32 163 39 546.6 0 39 164 48 546.6 0 48 165 79 546.6 0 79 166 67 546.6 0 67 167 45 546.6 0 45 168 54 546.6 0 54 169 40 546.6 0 40 170 56 546.6 0 56 171 51 546.6 0 51 172 101 546.6 0 101 173 303 546.6 0 303 174 124 546.6 0 124 175 308 546.6 0 308 176 67 546.6 0 67 177 55 546.6 0 55 178 44 546.6 0 44 179 33 546.6 0 33 180 66 546.6 0 66 181 47 546.6 0 47 182 37 546.6 0 37 183 41 546.6 0 41 184 46 546.6 0 46 185 77 546.6 0 77 186 72 546.6 0 72 187 72 546.6 0 72 188 42 546.6 0 42 189 51 546.6 0 51 190 67 546.6 0 67 191 127 546.6 0 127 192 458 546.6 0 458 193 302 546.6 0 302 194 547 546.6 0 547 195 125 546.6 0 125 196 40 546.6 0 40 197 56 546.6 0 56 198 52 546.6 0 52 199 63 546.6 0 63 200 81 546.6 0 81 201 64 546.6 0 64 202 51 546.6 0 51 203 59 546.6 0 59 204 92 546.6 0 92 205 72 546.6 0 72 206 62 546.6 0 62 207 96 546.6 0 96 208 50 546.6 0 50 209 112 546.6 0 112 210 90 546.6 0 90 211 174 546.6 0 174 212 143 546.6 0 143 213 58 546.6 0 58 214 53 546.6 0 53 215 81 546.6 0 81 216 38 546.6 0 38 217 87 546.6 0 87 218 61 546.6 0 61 219 92 546.6 0 92 220 99 546.6 0 99 221 92 546.6 0 92 222 53 546.6 0 53 223 53 546.6 0 53 224 83 546.6 0 83 225 281 546.6 0 281 226 84 546.6 0 84 227 182 546.6 0 182 228 109 546.6 0 109 229 59 546.6 0 59 230 62 546.6 0 62 231 64 546.6 0 64 232 72 546.6 0 72 233 75 546.6 0 75 234 56 546.6 0 56 235 60 546.6 0 60 236 73 546.6 0 73 237 81 546.6 0 81 238 81 546.6 0 81 239 62 546.6 0 62 240 91 546.6 0 91 241 111 546.6 0 111 242 86 546.6 0 86 243 62 546.6 0 62 244 43 546.6 0 43 245 53 546.6 0 53 246 84 546.6 0 84 247 86 546.6 0 86 248 94 546.6 0 94 249 101 546.6 0 101 250 56 546.6 0 56 251 49 546.6 0 49 252 60 546.6 0 60 253 54 546.6 0 54 254 124 546.6 0 124 255 179 546.6 0 179 256 129 546.6 0 129 257 83 546.6 0 83 258 107 546.6 0 107 259 129 546.6 0 129 260 85 546.6 0 85 261 90 546.6 0 90 262 69 546.6 0 69 263 97 546.6 0 97 264 74 546.6 0 74 265 86 546.6 0 86 266 99 546.6 0 99 267 150 546.6 0 150 268 153 546.6 0 153 269 93 546.6 0 93 270 97 546.6 0 97 271 208 546.6 0 208 272 250 546.6 0 250 273 212 546.6 0 212 274 397 546.6 0 397 275 181 546.6 0 181 276 114 546.6 0 114 277 83 546.6 0 83 278 122 546.6 0 122 279 244 546.6 0 244 280 2515 546.6 0 2515 281 151 546.6 0 151 282 105 546.6 0 105 283 76 546.6 0 76 284 48 546.6 0 48 285 109 546.6 0 109 286 108 546.6 0 108 287 92 546.6 0 92 288 146 546.6 0 146 289 71 546.6 0 71 290 125 546.6 0 125 291 95 546.6 0 95 292 90 546.6 0 90 293 101 546.6 0 101 294 178 546.6 0 178 295 151 546.6 0 151 296 247 546.6 0 247 297 96 546.6 0 96 298 92 546.6 0 92 299 85 546.6 0 85 300 91 546.6 0 91 301 51 546.6 0 51 Finished in 9.36 s (43 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 215,940 Reads with adapters: 114,439 (53.0%) Reads that were too short: 106 (0.0%) Reads written (passing filters): 114,333 (52.9%) Total basepairs processed: 61,584,431 bp Total written (filtered): 31,654,744 bp (51.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 114439 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 13 0.0 2 0 0 0 13 15 47 0.0 3 0 0 26 21 16 738 0.0 3 0 563 125 50 17 112053 0.0 3 105932 5058 863 200 18 624 0.0 3 10 193 26 395 19 115 0.0 3 0 0 59 56 20 648 0.0 3 1 0 0 647 21 1 0.0 3 0 0 0 1 22 1 0.0 3 0 0 0 1 24 1 0.0 3 1 25 1 0.0 3 1 26 1 0.0 3 0 0 0 1 27 2 0.0 3 1 0 0 1 29 1 0.0 3 1 30 1 0.0 3 1 31 3 0.0 3 2 0 0 1 33 2 0.0 3 0 0 0 2 38 5 0.0 3 0 0 0 5 40 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 1 46 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 51 2 0.0 3 0 0 0 2 53 1 0.0 3 0 0 0 1 55 5 0.0 3 0 0 1 4 56 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 1 63 2 0.0 3 0 0 0 2 64 3 0.0 3 0 0 0 3 65 3 0.0 3 0 0 0 3 67 4 0.0 3 0 0 0 4 68 17 0.0 3 0 0 0 17 69 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 71 2 0.0 3 0 0 0 2 74 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 1 78 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 88 2 0.0 3 0 0 0 2 91 2 0.0 3 0 0 0 2 93 2 0.0 3 0 0 0 2 99 3 0.0 3 0 0 1 2 101 1 0.0 3 0 0 0 1 104 2 0.0 3 0 0 0 2 106 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 156 2 0.0 3 0 0 0 2 157 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 164 3 0.0 3 0 0 0 3 170 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 183 6 0.0 3 0 0 0 6 187 5 0.0 3 0 0 1 4 191 1 0.0 3 0 0 0 1 192 13 0.0 3 0 0 0 13 194 8 0.0 3 0 0 0 8 195 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 0 1 206 2 0.0 3 0 0 0 2 215 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 218 2 0.0 3 0 0 0 2 221 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 228 1 0.0 3 0 0 0 1 237 2 0.0 3 0 0 0 2 238 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 14 0.0 3 0 0 0 14 280 2 0.0 3 0 0 0 2 281 14 0.0 3 0 0 1 13 282 7 0.0 3 0 0 0 7 283 1 0.0 3 0 0 0 1 284 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 287 1 0.0 3 0 0 0 1 288 3 0.0 3 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.97 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 114,333 Reads with adapters: 782 (0.7%) Reads written (passing filters): 114,333 (100.0%) Total basepairs processed: 31,654,744 bp Total written (filtered): 31,652,329 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 782 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 25.8% C: 47.1% G: 21.7% T: 5.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 739 1786.5 0 739 4 24 446.6 0 24 5 12 111.7 0 12 6 7 27.9 0 7