This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATGGAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 59.58 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 287,866 (12.9%) Reads that were too short: 2,500 (0.1%) Reads written (passing filters): 285,366 (12.7%) Total basepairs processed: 673,916,425 bp Total written (filtered): 81,593,953 bp (12.1%) === Adapter 1 === Sequence: ATGGAT; Type: regular 5'; Length: 6; Trimmed: 287866 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1611 546.6 0 1611 7 146501 546.6 0 146501 8 1609 546.6 0 1609 9 112666 546.6 0 112666 10 71 546.6 0 71 11 32 546.6 0 32 12 30 546.6 0 30 13 29 546.6 0 29 14 29 546.6 0 29 15 32 546.6 0 32 16 62 546.6 0 62 17 71 546.6 0 71 18 64 546.6 0 64 19 24 546.6 0 24 20 36 546.6 0 36 21 28 546.6 0 28 22 19 546.6 0 19 23 31 546.6 0 31 24 32 546.6 0 32 25 22 546.6 0 22 26 21 546.6 0 21 27 26 546.6 0 26 28 26 546.6 0 26 29 25 546.6 0 25 30 18 546.6 0 18 31 25 546.6 0 25 32 30 546.6 0 30 33 25 546.6 0 25 34 21 546.6 0 21 35 21 546.6 0 21 36 38 546.6 0 38 37 24 546.6 0 24 38 42 546.6 0 42 39 30 546.6 0 30 40 26 546.6 0 26 41 32 546.6 0 32 42 38 546.6 0 38 43 35 546.6 0 35 44 29 546.6 0 29 45 31 546.6 0 31 46 68 546.6 0 68 47 537 546.6 0 537 48 182 546.6 0 182 49 31 546.6 0 31 50 22 546.6 0 22 51 42 546.6 0 42 52 38 546.6 0 38 53 20 546.6 0 20 54 18 546.6 0 18 55 27 546.6 0 27 56 32 546.6 0 32 57 16 546.6 0 16 58 28 546.6 0 28 59 25 546.6 0 25 60 33 546.6 0 33 61 19 546.6 0 19 62 33 546.6 0 33 63 32 546.6 0 32 64 33 546.6 0 33 65 28 546.6 0 28 66 25 546.6 0 25 67 36 546.6 0 36 68 28 546.6 0 28 69 30 546.6 0 30 70 38 546.6 0 38 71 29 546.6 0 29 72 27 546.6 0 27 73 30 546.6 0 30 74 31 546.6 0 31 75 38 546.6 0 38 76 36 546.6 0 36 77 48 546.6 0 48 78 47 546.6 0 47 79 232 546.6 0 232 80 89 546.6 0 89 81 249 546.6 0 249 82 65 546.6 0 65 83 156 546.6 0 156 84 65 546.6 0 65 85 50 546.6 0 50 86 46 546.6 0 46 87 84 546.6 0 84 88 62 546.6 0 62 89 61 546.6 0 61 90 69 546.6 0 69 91 90 546.6 0 90 92 112 546.6 0 112 93 177 546.6 0 177 94 3034 546.6 0 3034 95 5001 546.6 0 5001 96 4640 546.6 0 4640 97 681 546.6 0 681 98 538 546.6 0 538 99 22 546.6 0 22 100 29 546.6 0 29 101 32 546.6 0 32 102 23 546.6 0 23 103 20 546.6 0 20 104 33 546.6 0 33 105 20 546.6 0 20 106 24 546.6 0 24 107 40 546.6 0 40 108 26 546.6 0 26 109 33 546.6 0 33 110 94 546.6 0 94 111 72 546.6 0 72 112 142 546.6 0 142 113 34 546.6 0 34 114 31 546.6 0 31 115 30 546.6 0 30 116 31 546.6 0 31 117 29 546.6 0 29 118 118 546.6 0 118 119 39 546.6 0 39 120 38 546.6 0 38 121 14 546.6 0 14 122 28 546.6 0 28 123 19 546.6 0 19 124 61 546.6 0 61 125 125 546.6 0 125 126 158 546.6 0 158 127 25 546.6 0 25 128 29 546.6 0 29 129 38 546.6 0 38 130 95 546.6 0 95 131 67 546.6 0 67 132 57 546.6 0 57 133 99 546.6 0 99 134 33 546.6 0 33 135 40 546.6 0 40 136 50 546.6 0 50 137 119 546.6 0 119 138 269 546.6 0 269 139 232 546.6 0 232 140 35 546.6 0 35 141 94 546.6 0 94 142 69 546.6 0 69 143 139 546.6 0 139 144 79 546.6 0 79 145 85 546.6 0 85 146 98 546.6 0 98 147 36 546.6 0 36 148 58 546.6 0 58 149 24 546.6 0 24 150 10 546.6 0 10 151 17 546.6 0 17 152 14 546.6 0 14 153 11 546.6 0 11 154 19 546.6 0 19 155 11 546.6 0 11 156 22 546.6 0 22 157 25 546.6 0 25 158 220 546.6 0 220 159 78 546.6 0 78 160 105 546.6 0 105 161 16 546.6 0 16 162 19 546.6 0 19 163 16 546.6 0 16 164 13 546.6 0 13 165 24 546.6 0 24 166 28 546.6 0 28 167 17 546.6 0 17 168 14 546.6 0 14 169 9 546.6 0 9 170 20 546.6 0 20 171 22 546.6 0 22 172 19 546.6 0 19 173 6 546.6 0 6 174 13 546.6 0 13 175 41 546.6 0 41 176 9 546.6 0 9 177 15 546.6 0 15 178 23 546.6 0 23 179 20 546.6 0 20 180 23 546.6 0 23 181 35 546.6 0 35 182 14 546.6 0 14 183 21 546.6 0 21 184 13 546.6 0 13 185 17 546.6 0 17 186 70 546.6 0 70 187 18 546.6 0 18 188 19 546.6 0 19 189 16 546.6 0 16 190 22 546.6 0 22 191 16 546.6 0 16 192 43 546.6 0 43 193 21 546.6 0 21 194 26 546.6 0 26 195 24 546.6 0 24 196 18 546.6 0 18 197 12 546.6 0 12 198 11 546.6 0 11 199 13 546.6 0 13 200 19 546.6 0 19 201 14 546.6 0 14 202 23 546.6 0 23 203 16 546.6 0 16 204 14 546.6 0 14 205 79 546.6 0 79 206 40 546.6 0 40 207 65 546.6 0 65 208 17 546.6 0 17 209 10 546.6 0 10 210 15 546.6 0 15 211 20 546.6 0 20 212 13 546.6 0 13 213 18 546.6 0 18 214 14 546.6 0 14 215 9 546.6 0 9 216 16 546.6 0 16 217 14 546.6 0 14 218 24 546.6 0 24 219 15 546.6 0 15 220 19 546.6 0 19 221 22 546.6 0 22 222 14 546.6 0 14 223 20 546.6 0 20 224 16 546.6 0 16 225 16 546.6 0 16 226 10 546.6 0 10 227 10 546.6 0 10 228 20 546.6 0 20 229 17 546.6 0 17 230 13 546.6 0 13 231 13 546.6 0 13 232 12 546.6 0 12 233 23 546.6 0 23 234 18 546.6 0 18 235 13 546.6 0 13 236 23 546.6 0 23 237 15 546.6 0 15 238 20 546.6 0 20 239 20 546.6 0 20 240 13 546.6 0 13 241 21 546.6 0 21 242 16 546.6 0 16 243 10 546.6 0 10 244 18 546.6 0 18 245 25 546.6 0 25 246 16 546.6 0 16 247 15 546.6 0 15 248 16 546.6 0 16 249 21 546.6 0 21 250 17 546.6 0 17 251 78 546.6 0 78 252 10 546.6 0 10 253 21 546.6 0 21 254 26 546.6 0 26 255 19 546.6 0 19 256 23 546.6 0 23 257 19 546.6 0 19 258 20 546.6 0 20 259 24 546.6 0 24 260 19 546.6 0 19 261 48 546.6 0 48 262 51 546.6 0 51 263 34 546.6 0 34 264 26 546.6 0 26 265 20 546.6 0 20 266 38 546.6 0 38 267 24 546.6 0 24 268 20 546.6 0 20 269 10 546.6 0 10 270 23 546.6 0 23 271 31 546.6 0 31 272 25 546.6 0 25 273 21 546.6 0 21 274 30 546.6 0 30 275 20 546.6 0 20 276 29 546.6 0 29 277 19 546.6 0 19 278 21 546.6 0 21 279 26 546.6 0 26 280 19 546.6 0 19 281 37 546.6 0 37 282 58 546.6 0 58 283 50 546.6 0 50 284 29 546.6 0 29 285 44 546.6 0 44 286 69 546.6 0 69 287 34 546.6 0 34 288 38 546.6 0 38 289 32 546.6 0 32 290 26 546.6 0 26 291 40 546.6 0 40 292 20 546.6 0 20 293 16 546.6 0 16 294 21 546.6 0 21 295 31 546.6 0 31 296 27 546.6 0 27 297 29 546.6 0 29 298 35 546.6 0 35 299 46 546.6 0 46 300 43 546.6 0 43 301 37 546.6 0 37 Finished in 13.26 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 285,366 Reads with adapters: 146,860 (51.5%) Reads that were too short: 63 (0.0%) Reads written (passing filters): 146,797 (51.4%) Total basepairs processed: 81,593,953 bp Total written (filtered): 40,189,379 bp (49.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 146860 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 4 0.0 3 0 0 0 2 2 18 28 0.0 3 0 0 28 19 1080 0.0 3 0 1049 22 4 5 20 144895 0.0 4 142466 2329 83 15 2 21 371 0.0 4 27 341 2 1 22 14 0.0 4 2 0 10 0 2 23 76 0.0 4 0 0 1 63 12 24 9 0.0 4 6 0 0 2 1 25 1 0.0 4 1 28 2 0.0 4 0 1 0 0 1 33 2 0.0 4 2 37 1 0.0 4 1 41 1 0.0 4 1 117 1 0.0 4 0 0 0 0 1 124 1 0.0 4 1 126 6 0.0 4 0 0 0 0 6 127 297 0.0 4 0 0 0 0 297 131 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 138 1 0.0 4 1 140 1 0.0 4 0 0 0 0 1 141 26 0.0 4 0 0 0 2 24 142 6 0.0 4 0 0 0 0 6 143 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 0 0 0 1 179 1 0.0 4 0 0 0 0 1 189 1 0.0 4 0 0 0 0 1 222 1 0.0 4 0 0 0 0 1 228 6 0.0 4 0 0 0 0 6 229 3 0.0 4 1 0 0 0 2 230 1 0.0 4 0 0 0 0 1 231 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 234 2 0.0 4 0 0 0 0 2 236 1 0.0 4 0 0 0 0 1 237 3 0.0 4 0 0 0 0 3 240 1 0.0 4 0 0 1 260 1 0.0 4 0 0 0 0 1 266 1 0.0 4 0 0 0 0 1 267 1 0.0 4 0 0 0 0 1 280 1 0.0 4 0 0 0 0 1 284 2 0.0 4 0 0 0 0 2 292 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 5.01 s (34 us/read; 1.76 M reads/minute). === Summary === Total reads processed: 146,797 Reads with adapters: 5,528 (3.8%) Reads written (passing filters): 146,797 (100.0%) Total basepairs processed: 40,189,379 bp Total written (filtered): 40,148,257 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 5528 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.9% C: 3.6% G: 89.6% T: 1.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 276 2293.7 0 276 4 3994 573.4 0 3994 5 5 143.4 0 5 6 8 35.8 0 8 7 108 9.0 0 108 9 10 0.6 0 3 7 10 43 0.1 1 0 43 11 749 0.0 1 8 741 12 254 0.0 1 1 253 13 8 0.0 1 0 8 14 2 0.0 1 0 2 15 5 0.0 1 0 5 16 1 0.0 1 0 1 72 1 0.0 1 1 78 1 0.0 1 1 93 1 0.0 1 1 110 1 0.0 1 1 113 1 0.0 1 1 149 1 0.0 1 1 152 19 0.0 1 0 19 153 1 0.0 1 0 1 157 3 0.0 1 3 158 1 0.0 1 1 160 2 0.0 1 0 2 198 14 0.0 1 9 5 210 16 0.0 1 10 6 229 1 0.0 1 1 234 1 0.0 1 1 259 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.