This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCTGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 135.04 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 321,049 (10.9%) Reads that were too short: 21,016 (0.7%) Reads written (passing filters): 300,033 (10.2%) Total basepairs processed: 887,907,860 bp Total written (filtered): 55,165,126 bp (6.2%) === Adapter 1 === Sequence: CTCTGC; Type: regular 5'; Length: 6; Trimmed: 321049 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 472 720.2 0 472 7 12294 720.2 0 12294 8 322 720.2 0 322 9 8660 720.2 0 8660 10 30 720.2 0 30 11 18 720.2 0 18 12 21 720.2 0 21 13 33 720.2 0 33 14 77 720.2 0 77 15 48 720.2 0 48 16 77 720.2 0 77 17 18 720.2 0 18 18 22 720.2 0 22 19 32 720.2 0 32 20 29 720.2 0 29 21 30 720.2 0 30 22 37 720.2 0 37 23 42 720.2 0 42 24 108 720.2 0 108 25 51 720.2 0 51 26 34 720.2 0 34 27 40 720.2 0 40 28 37 720.2 0 37 29 32 720.2 0 32 30 31 720.2 0 31 31 32 720.2 0 32 32 34 720.2 0 34 33 80 720.2 0 80 34 70 720.2 0 70 35 53 720.2 0 53 36 43 720.2 0 43 37 50 720.2 0 50 38 48 720.2 0 48 39 86 720.2 0 86 40 115 720.2 0 115 41 118 720.2 0 118 42 33 720.2 0 33 43 47 720.2 0 47 44 42 720.2 0 42 45 39 720.2 0 39 46 53 720.2 0 53 47 39 720.2 0 39 48 52 720.2 0 52 49 36 720.2 0 36 50 41 720.2 0 41 51 55 720.2 0 55 52 72 720.2 0 72 53 52 720.2 0 52 54 46 720.2 0 46 55 42 720.2 0 42 56 123 720.2 0 123 57 165 720.2 0 165 58 178 720.2 0 178 59 396 720.2 0 396 60 119 720.2 0 119 61 332 720.2 0 332 62 34 720.2 0 34 63 40 720.2 0 40 64 56 720.2 0 56 65 51 720.2 0 51 66 26 720.2 0 26 67 90 720.2 0 90 68 33 720.2 0 33 69 51 720.2 0 51 70 69 720.2 0 69 71 41 720.2 0 41 72 39 720.2 0 39 73 72 720.2 0 72 74 49 720.2 0 49 75 49 720.2 0 49 76 51 720.2 0 51 77 237 720.2 0 237 78 66 720.2 0 66 79 35 720.2 0 35 80 72 720.2 0 72 81 57 720.2 0 57 82 49 720.2 0 49 83 84 720.2 0 84 84 116 720.2 0 116 85 104 720.2 0 104 86 88 720.2 0 88 87 404 720.2 0 404 88 63 720.2 0 63 89 95 720.2 0 95 90 93 720.2 0 93 91 116 720.2 0 116 92 113 720.2 0 113 93 111 720.2 0 111 94 191 720.2 0 191 95 119 720.2 0 119 96 189 720.2 0 189 97 501 720.2 0 501 98 138 720.2 0 138 99 223 720.2 0 223 100 182 720.2 0 182 101 216 720.2 0 216 102 345 720.2 0 345 103 185 720.2 0 185 104 136 720.2 0 136 105 152 720.2 0 152 106 527 720.2 0 527 107 824 720.2 0 824 108 645 720.2 0 645 109 577 720.2 0 577 110 836 720.2 0 836 111 977 720.2 0 977 112 2723 720.2 0 2723 113 2760 720.2 0 2760 114 49169 720.2 0 49169 115 32524 720.2 0 32524 116 82669 720.2 0 82669 117 434 720.2 0 434 118 185 720.2 0 185 119 52 720.2 0 52 120 76 720.2 0 76 121 38 720.2 0 38 122 39 720.2 0 39 123 46 720.2 0 46 124 51 720.2 0 51 125 50 720.2 0 50 126 119 720.2 0 119 127 243 720.2 0 243 128 161 720.2 0 161 129 295 720.2 0 295 130 192 720.2 0 192 131 141 720.2 0 141 132 70 720.2 0 70 133 54 720.2 0 54 134 68 720.2 0 68 135 96 720.2 0 96 136 150 720.2 0 150 137 154 720.2 0 154 138 89 720.2 0 89 139 102 720.2 0 102 140 504 720.2 0 504 141 1000 720.2 0 1000 142 1014 720.2 0 1014 143 623 720.2 0 623 144 545 720.2 0 545 145 679 720.2 0 679 146 875 720.2 0 875 147 11947 720.2 0 11947 148 15839 720.2 0 15839 149 38610 720.2 0 38610 150 540 720.2 0 540 151 270 720.2 0 270 152 299 720.2 0 299 153 4317 720.2 0 4317 154 2549 720.2 0 2549 155 5064 720.2 0 5064 156 422 720.2 0 422 157 476 720.2 0 476 158 333 720.2 0 333 159 64 720.2 0 64 160 159 720.2 0 159 161 271 720.2 0 271 162 265 720.2 0 265 163 390 720.2 0 390 164 130 720.2 0 130 165 90 720.2 0 90 166 52 720.2 0 52 167 76 720.2 0 76 168 265 720.2 0 265 169 145 720.2 0 145 170 329 720.2 0 329 171 313 720.2 0 313 172 43 720.2 0 43 173 43 720.2 0 43 174 38 720.2 0 38 175 51 720.2 0 51 176 80 720.2 0 80 177 63 720.2 0 63 178 54 720.2 0 54 179 36 720.2 0 36 180 33 720.2 0 33 181 142 720.2 0 142 182 117 720.2 0 117 183 357 720.2 0 357 184 331 720.2 0 331 185 510 720.2 0 510 186 747 720.2 0 747 187 209 720.2 0 209 188 199 720.2 0 199 189 56 720.2 0 56 190 86 720.2 0 86 191 95 720.2 0 95 192 127 720.2 0 127 193 60 720.2 0 60 194 49 720.2 0 49 195 32 720.2 0 32 196 48 720.2 0 48 197 106 720.2 0 106 198 74 720.2 0 74 199 53 720.2 0 53 200 49 720.2 0 49 201 37 720.2 0 37 202 96 720.2 0 96 203 64 720.2 0 64 204 161 720.2 0 161 205 35 720.2 0 35 206 50 720.2 0 50 207 49 720.2 0 49 208 25 720.2 0 25 209 32 720.2 0 32 210 23 720.2 0 23 211 33 720.2 0 33 212 49 720.2 0 49 213 59 720.2 0 59 214 46 720.2 0 46 215 67 720.2 0 67 216 333 720.2 0 333 217 199 720.2 0 199 218 456 720.2 0 456 219 40 720.2 0 40 220 30 720.2 0 30 221 43 720.2 0 43 222 57 720.2 0 57 223 34 720.2 0 34 224 45 720.2 0 45 225 38 720.2 0 38 226 87 720.2 0 87 227 81 720.2 0 81 228 133 720.2 0 133 229 43 720.2 0 43 230 50 720.2 0 50 231 61 720.2 0 61 232 51 720.2 0 51 233 68 720.2 0 68 234 142 720.2 0 142 235 235 720.2 0 235 236 258 720.2 0 258 237 157 720.2 0 157 238 227 720.2 0 227 239 52 720.2 0 52 240 42 720.2 0 42 241 43 720.2 0 43 242 56 720.2 0 56 243 40 720.2 0 40 244 79 720.2 0 79 245 90 720.2 0 90 246 122 720.2 0 122 247 89 720.2 0 89 248 59 720.2 0 59 249 103 720.2 0 103 250 191 720.2 0 191 251 176 720.2 0 176 252 58 720.2 0 58 253 70 720.2 0 70 254 100 720.2 0 100 255 77 720.2 0 77 256 59 720.2 0 59 257 98 720.2 0 98 258 61 720.2 0 61 259 90 720.2 0 90 260 48 720.2 0 48 261 94 720.2 0 94 262 105 720.2 0 105 263 76 720.2 0 76 264 63 720.2 0 63 265 81 720.2 0 81 266 72 720.2 0 72 267 85 720.2 0 85 268 109 720.2 0 109 269 116 720.2 0 116 270 260 720.2 0 260 271 330 720.2 0 330 272 268 720.2 0 268 273 130 720.2 0 130 274 117 720.2 0 117 275 139 720.2 0 139 276 202 720.2 0 202 277 183 720.2 0 183 278 182 720.2 0 182 279 1243 720.2 0 1243 280 746 720.2 0 746 281 2417 720.2 0 2417 282 2083 720.2 0 2083 283 2099 720.2 0 2099 284 151 720.2 0 151 285 220 720.2 0 220 286 273 720.2 0 273 287 123 720.2 0 123 288 75 720.2 0 75 289 116 720.2 0 116 290 432 720.2 0 432 291 475 720.2 0 475 292 608 720.2 0 608 293 262 720.2 0 262 294 216 720.2 0 216 295 308 720.2 0 308 296 277 720.2 0 277 297 299 720.2 0 299 298 297 720.2 0 297 299 111 720.2 0 111 300 52 720.2 0 52 301 61 720.2 0 61 Finished in 15.95 s (53 us/read; 1.13 M reads/minute). === Summary === Total reads processed: 300,033 Reads with adapters: 12,772 (4.3%) Reads that were too short: 193 (0.1%) Reads written (passing filters): 12,579 (4.2%) Total basepairs processed: 55,165,126 bp Total written (filtered): 3,448,015 bp (6.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 12772 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 6 0.0 3 0 0 4 2 16 111 0.0 3 0 89 11 11 17 12127 0.0 3 11498 511 92 26 18 41 0.0 3 5 31 3 2 19 25 0.0 3 0 0 18 7 20 46 0.0 3 0 0 0 46 21 1 0.0 3 0 0 0 1 22 3 0.0 3 0 0 0 3 27 4 0.0 3 0 0 0 4 28 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 1 32 2 0.0 3 0 0 0 2 33 1 0.0 3 0 0 0 1 39 1 0.0 3 1 40 3 0.0 3 0 0 0 3 41 3 0.0 3 0 0 0 3 42 5 0.0 3 0 0 0 5 44 2 0.0 3 0 0 0 2 45 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 48 2 0.0 3 0 0 0 2 49 3 0.0 3 0 0 0 3 50 1 0.0 3 0 0 0 1 51 65 0.0 3 0 0 0 65 52 1 0.0 3 0 0 1 53 1 0.0 3 0 0 0 1 54 8 0.0 3 0 0 0 8 57 4 0.0 3 0 0 0 4 58 3 0.0 3 0 0 0 3 59 1 0.0 3 0 0 0 1 60 9 0.0 3 0 0 0 9 61 1 0.0 3 0 0 0 1 63 4 0.0 3 0 0 0 4 64 1 0.0 3 0 0 0 1 66 3 0.0 3 0 0 0 3 68 2 0.0 3 0 0 0 2 70 2 0.0 3 0 0 0 2 74 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 77 3 0.0 3 0 0 0 3 78 1 0.0 3 0 0 1 82 1 0.0 3 0 0 0 1 84 2 0.0 3 0 0 0 2 85 51 0.0 3 0 0 0 51 86 5 0.0 3 0 0 0 5 87 1 0.0 3 0 0 0 1 88 10 0.0 3 0 0 1 9 89 4 0.0 3 0 0 0 4 90 2 0.0 3 0 0 0 2 92 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 98 4 0.0 3 0 0 0 4 100 1 0.0 3 0 0 0 1 104 2 0.0 3 0 0 0 2 106 1 0.0 3 0 1 108 2 0.0 3 0 0 0 2 109 1 0.0 3 0 0 0 1 110 4 0.0 3 0 0 1 3 111 2 0.0 3 0 0 0 2 112 2 0.0 3 0 0 0 2 113 1 0.0 3 0 0 0 1 114 2 0.0 3 0 0 0 2 116 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 131 3 0.0 3 0 0 0 3 133 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 1 135 7 0.0 3 0 0 0 7 136 7 0.0 3 0 0 1 6 137 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 139 2 0.0 3 0 0 0 2 140 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 144 2 0.0 3 0 0 0 2 147 2 0.0 3 0 0 0 2 148 2 0.0 3 0 0 0 2 149 1 0.0 3 0 0 0 1 152 4 0.0 3 0 0 1 3 154 3 0.0 3 0 0 0 3 156 2 0.0 3 0 0 0 2 157 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 164 2 0.0 3 0 0 0 2 167 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 172 13 0.0 3 0 0 0 13 173 2 0.0 3 0 0 0 2 174 36 0.0 3 0 0 1 35 175 19 0.0 3 0 0 0 19 176 1 0.0 3 0 0 0 1 177 2 0.0 3 0 0 0 2 181 3 0.0 3 0 0 0 3 182 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 186 3 0.0 3 0 0 0 3 187 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 191 2 0.0 3 0 0 0 2 194 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 0 1 200 1 0.0 3 1 204 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 1 216 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 1 221 1 0.0 3 0 0 0 1 223 2 0.0 3 0 0 0 2 229 1 0.0 3 0 0 0 1 242 1 0.0 3 0 0 0 1 249 3 0.0 3 0 0 0 3 280 3 0.0 3 0 0 0 3 283 1 0.0 3 0 0 1 284 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.68 s (54 us/read; 1.11 M reads/minute). === Summary === Total reads processed: 12,579 Reads with adapters: 90 (0.7%) Reads written (passing filters): 12,579 (100.0%) Total basepairs processed: 3,448,015 bp Total written (filtered): 3,447,735 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 90 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 27.8% C: 42.2% G: 21.1% T: 8.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 82 196.5 0 82 4 6 49.1 0 6 5 2 12.3 0 2