This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGTGGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 130.32 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 289,385 (9.8%) Reads that were too short: 98,988 (3.4%) Reads written (passing filters): 190,397 (6.5%) Total basepairs processed: 887,907,860 bp Total written (filtered): 54,152,719 bp (6.1%) === Adapter 1 === Sequence: TGTGGC; Type: regular 5'; Length: 6; Trimmed: 289385 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3031 720.2 0 3031 7 71448 720.2 0 71448 8 2134 720.2 0 2134 9 98326 720.2 0 98326 10 102 720.2 0 102 11 41 720.2 0 41 12 183 720.2 0 183 13 24 720.2 0 24 14 29 720.2 0 29 15 35 720.2 0 35 16 26 720.2 0 26 17 112 720.2 0 112 18 32 720.2 0 32 19 57 720.2 0 57 20 40 720.2 0 40 21 29 720.2 0 29 22 35 720.2 0 35 23 35 720.2 0 35 24 27 720.2 0 27 25 32 720.2 0 32 26 33 720.2 0 33 27 43 720.2 0 43 28 40 720.2 0 40 29 34 720.2 0 34 30 49 720.2 0 49 31 61 720.2 0 61 32 46 720.2 0 46 33 49 720.2 0 49 34 33 720.2 0 33 35 27 720.2 0 27 36 33 720.2 0 33 37 42 720.2 0 42 38 62 720.2 0 62 39 35 720.2 0 35 40 46 720.2 0 46 41 40 720.2 0 40 42 39 720.2 0 39 43 26 720.2 0 26 44 30 720.2 0 30 45 37 720.2 0 37 46 39 720.2 0 39 47 49 720.2 0 49 48 35 720.2 0 35 49 66 720.2 0 66 50 37 720.2 0 37 51 63 720.2 0 63 52 53 720.2 0 53 53 148 720.2 0 148 54 85 720.2 0 85 55 64 720.2 0 64 56 39 720.2 0 39 57 391 720.2 0 391 58 51 720.2 0 51 59 51 720.2 0 51 60 62 720.2 0 62 61 47 720.2 0 47 62 39 720.2 0 39 63 53 720.2 0 53 64 67 720.2 0 67 65 71 720.2 0 71 66 35 720.2 0 35 67 47 720.2 0 47 68 69 720.2 0 69 69 61 720.2 0 61 70 51 720.2 0 51 71 361 720.2 0 361 72 148 720.2 0 148 73 167 720.2 0 167 74 37 720.2 0 37 75 38 720.2 0 38 76 29 720.2 0 29 77 39 720.2 0 39 78 42 720.2 0 42 79 48 720.2 0 48 80 35 720.2 0 35 81 57 720.2 0 57 82 55 720.2 0 55 83 38 720.2 0 38 84 45 720.2 0 45 85 53 720.2 0 53 86 43 720.2 0 43 87 28 720.2 0 28 88 21 720.2 0 21 89 49 720.2 0 49 90 28 720.2 0 28 91 60 720.2 0 60 92 123 720.2 0 123 93 67 720.2 0 67 94 56 720.2 0 56 95 66 720.2 0 66 96 64 720.2 0 64 97 44 720.2 0 44 98 58 720.2 0 58 99 45 720.2 0 45 100 38 720.2 0 38 101 35 720.2 0 35 102 116 720.2 0 116 103 50 720.2 0 50 104 31 720.2 0 31 105 34 720.2 0 34 106 38 720.2 0 38 107 30 720.2 0 30 108 32 720.2 0 32 109 47 720.2 0 47 110 40 720.2 0 40 111 43 720.2 0 43 112 28 720.2 0 28 113 33 720.2 0 33 114 53 720.2 0 53 115 41 720.2 0 41 116 42 720.2 0 42 117 74 720.2 0 74 118 44 720.2 0 44 119 34 720.2 0 34 120 105 720.2 0 105 121 64 720.2 0 64 122 69 720.2 0 69 123 1032 720.2 0 1032 124 82 720.2 0 82 125 39 720.2 0 39 126 59 720.2 0 59 127 44 720.2 0 44 128 72 720.2 0 72 129 97 720.2 0 97 130 1381 720.2 0 1381 131 639 720.2 0 639 132 349 720.2 0 349 133 240 720.2 0 240 134 99 720.2 0 99 135 39 720.2 0 39 136 81 720.2 0 81 137 408 720.2 0 408 138 724 720.2 0 724 139 248 720.2 0 248 140 49 720.2 0 49 141 53 720.2 0 53 142 62 720.2 0 62 143 36 720.2 0 36 144 46 720.2 0 46 145 39 720.2 0 39 146 39 720.2 0 39 147 26 720.2 0 26 148 66 720.2 0 66 149 32 720.2 0 32 150 54 720.2 0 54 151 176 720.2 0 176 152 246 720.2 0 246 153 95 720.2 0 95 154 36 720.2 0 36 155 49 720.2 0 49 156 22 720.2 0 22 157 27 720.2 0 27 158 43 720.2 0 43 159 39 720.2 0 39 160 30 720.2 0 30 161 22 720.2 0 22 162 26 720.2 0 26 163 46 720.2 0 46 164 79 720.2 0 79 165 52 720.2 0 52 166 30 720.2 0 30 167 45 720.2 0 45 168 27 720.2 0 27 169 26 720.2 0 26 170 28 720.2 0 28 171 22 720.2 0 22 172 25 720.2 0 25 173 23 720.2 0 23 174 23 720.2 0 23 175 40 720.2 0 40 176 30 720.2 0 30 177 81 720.2 0 81 178 113 720.2 0 113 179 51 720.2 0 51 180 35 720.2 0 35 181 46 720.2 0 46 182 45 720.2 0 45 183 53 720.2 0 53 184 43 720.2 0 43 185 40 720.2 0 40 186 90 720.2 0 90 187 129 720.2 0 129 188 126 720.2 0 126 189 105 720.2 0 105 190 63 720.2 0 63 191 50 720.2 0 50 192 29 720.2 0 29 193 39 720.2 0 39 194 54 720.2 0 54 195 37 720.2 0 37 196 56 720.2 0 56 197 64 720.2 0 64 198 41 720.2 0 41 199 59 720.2 0 59 200 52 720.2 0 52 201 33 720.2 0 33 202 47 720.2 0 47 203 54 720.2 0 54 204 63 720.2 0 63 205 94 720.2 0 94 206 71 720.2 0 71 207 41 720.2 0 41 208 47 720.2 0 47 209 38 720.2 0 38 210 60 720.2 0 60 211 75 720.2 0 75 212 476 720.2 0 476 213 743 720.2 0 743 214 403 720.2 0 403 215 61 720.2 0 61 216 54 720.2 0 54 217 114 720.2 0 114 218 107 720.2 0 107 219 51 720.2 0 51 220 64 720.2 0 64 221 51 720.2 0 51 222 51 720.2 0 51 223 42 720.2 0 42 224 56 720.2 0 56 225 55 720.2 0 55 226 45 720.2 0 45 227 35 720.2 0 35 228 61 720.2 0 61 229 44 720.2 0 44 230 51 720.2 0 51 231 60 720.2 0 60 232 72 720.2 0 72 233 148 720.2 0 148 234 255 720.2 0 255 235 183 720.2 0 183 236 568 720.2 0 568 237 359 720.2 0 359 238 360 720.2 0 360 239 122 720.2 0 122 240 65 720.2 0 65 241 95 720.2 0 95 242 175 720.2 0 175 243 427 720.2 0 427 244 818 720.2 0 818 245 716 720.2 0 716 246 397 720.2 0 397 247 211 720.2 0 211 248 150 720.2 0 150 249 92 720.2 0 92 250 105 720.2 0 105 251 101 720.2 0 101 252 106 720.2 0 106 253 77 720.2 0 77 254 88 720.2 0 88 255 205 720.2 0 205 256 211 720.2 0 211 257 372 720.2 0 372 258 316 720.2 0 316 259 266 720.2 0 266 260 726 720.2 0 726 261 1373 720.2 0 1373 262 739 720.2 0 739 263 209 720.2 0 209 264 157 720.2 0 157 265 174 720.2 0 174 266 316 720.2 0 316 267 250 720.2 0 250 268 137 720.2 0 137 269 110 720.2 0 110 270 137 720.2 0 137 271 205 720.2 0 205 272 294 720.2 0 294 273 538 720.2 0 538 274 216 720.2 0 216 275 423 720.2 0 423 276 385 720.2 0 385 277 256 720.2 0 256 278 278 720.2 0 278 279 345 720.2 0 345 280 404 720.2 0 404 281 749 720.2 0 749 282 529 720.2 0 529 283 629 720.2 0 629 284 853 720.2 0 853 285 1252 720.2 0 1252 286 19698 720.2 0 19698 287 39266 720.2 0 39266 288 15576 720.2 0 15576 289 119 720.2 0 119 290 83 720.2 0 83 291 121 720.2 0 121 292 143 720.2 0 143 293 93 720.2 0 93 294 92 720.2 0 92 295 234 720.2 0 234 296 163 720.2 0 163 297 385 720.2 0 385 298 464 720.2 0 464 299 436 720.2 0 436 300 107 720.2 0 107 301 50 720.2 0 50 Finished in 13.53 s (71 us/read; 0.84 M reads/minute). === Summary === Total reads processed: 190,397 Reads with adapters: 101,692 (53.4%) Reads that were too short: 262 (0.1%) Reads written (passing filters): 101,430 (53.3%) Total basepairs processed: 54,152,719 bp Total written (filtered): 27,881,098 bp (51.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 101692 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 9 0.0 2 0 0 0 9 15 38 0.0 3 0 0 24 14 16 683 0.0 3 0 579 73 31 17 99815 0.0 3 93510 5191 905 209 18 258 0.0 3 23 205 21 9 19 139 0.0 3 1 0 69 69 20 373 0.0 3 2 1 2 368 21 3 0.0 3 0 1 0 2 28 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 31 2 0.0 3 0 0 0 2 33 1 0.0 3 1 35 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 41 6 0.0 3 0 0 0 6 42 4 0.0 3 0 0 0 4 44 11 0.0 3 0 0 0 11 46 2 0.0 3 0 0 0 2 47 1 0.0 3 0 0 0 1 49 2 0.0 3 0 0 0 2 50 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 61 18 0.0 3 0 0 0 18 63 3 0.0 3 0 0 0 3 64 1 0.0 3 0 0 0 1 67 3 0.0 3 1 0 0 2 73 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 95 2 0.0 3 0 0 1 1 98 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 1 106 2 0.0 3 0 0 1 1 107 2 0.0 3 0 0 0 2 108 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 112 3 0.0 3 0 0 0 3 113 2 0.0 3 0 1 1 114 1 0.0 3 1 115 1 0.0 3 0 0 0 1 121 3 0.0 3 0 0 0 3 127 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 156 9 0.0 3 0 0 0 9 159 2 0.0 3 0 0 0 2 161 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 6 0.0 3 0 0 0 6 164 77 0.0 3 0 0 6 71 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 167 2 0.0 3 1 0 0 1 168 2 0.0 3 0 0 0 2 169 1 0.0 3 0 0 0 1 170 8 0.0 3 0 0 0 8 171 69 0.0 3 0 0 3 66 172 2 0.0 3 0 0 0 2 173 1 0.0 3 0 0 0 1 174 3 0.0 3 0 0 0 3 183 3 0.0 3 0 0 0 3 184 2 0.0 3 0 0 0 2 185 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 187 2 0.0 3 0 0 1 1 191 4 0.0 3 0 0 0 4 192 13 0.0 3 0 0 0 13 194 9 0.0 3 0 0 1 8 195 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 207 8 0.0 3 0 0 0 8 208 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 2 0.0 3 0 0 0 2 218 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 230 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 245 1 0.0 3 0 0 0 1 262 1 0.0 3 0 0 0 1 267 1 0.0 3 0 0 0 1 268 1 0.0 3 0 0 0 1 279 6 0.0 3 0 0 0 6 280 5 0.0 3 0 0 0 5 281 9 0.0 3 0 0 0 9 282 7 0.0 3 0 0 0 7 283 1 0.0 3 0 0 0 1 287 1 0.0 3 0 0 0 1 288 2 0.0 3 0 0 1 1 292 1 0.0 3 0 0 0 1 294 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 5.57 s (55 us/read; 1.09 M reads/minute). === Summary === Total reads processed: 101,430 Reads with adapters: 3,514 (3.5%) Reads written (passing filters): 101,430 (100.0%) Total basepairs processed: 27,881,098 bp Total written (filtered): 27,870,443 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 3514 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 62.6% C: 12.9% G: 22.8% T: 1.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 3424 1584.8 0 3424 4 85 396.2 0 85 5 2 99.1 0 2 10 2 0.1 1 0 2 13 1 0.0 1 0 1