This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGTGGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 130.16 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 303,470 (10.3%) Reads that were too short: 110,272 (3.7%) Reads written (passing filters): 193,198 (6.5%) Total basepairs processed: 887,907,860 bp Total written (filtered): 55,306,218 bp (6.2%) === Adapter 1 === Sequence: TGTGGC; Type: regular 5'; Length: 6; Trimmed: 303470 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3144 720.2 0 3144 7 100622 720.2 0 100622 8 1437 720.2 0 1437 9 74324 720.2 0 74324 10 76 720.2 0 76 11 45 720.2 0 45 12 125 720.2 0 125 13 32 720.2 0 32 14 36 720.2 0 36 15 44 720.2 0 44 16 35 720.2 0 35 17 48 720.2 0 48 18 43 720.2 0 43 19 35 720.2 0 35 20 53 720.2 0 53 21 39 720.2 0 39 22 32 720.2 0 32 23 37 720.2 0 37 24 41 720.2 0 41 25 36 720.2 0 36 26 62 720.2 0 62 27 42 720.2 0 42 28 44 720.2 0 44 29 33 720.2 0 33 30 44 720.2 0 44 31 29 720.2 0 29 32 55 720.2 0 55 33 48 720.2 0 48 34 41 720.2 0 41 35 48 720.2 0 48 36 58 720.2 0 58 37 49 720.2 0 49 38 61 720.2 0 61 39 39 720.2 0 39 40 64 720.2 0 64 41 54 720.2 0 54 42 57 720.2 0 57 43 41 720.2 0 41 44 46 720.2 0 46 45 46 720.2 0 46 46 33 720.2 0 33 47 45 720.2 0 45 48 37 720.2 0 37 49 62 720.2 0 62 50 40 720.2 0 40 51 59 720.2 0 59 52 66 720.2 0 66 53 296 720.2 0 296 54 103 720.2 0 103 55 64 720.2 0 64 56 49 720.2 0 49 57 412 720.2 0 412 58 67 720.2 0 67 59 45 720.2 0 45 60 45 720.2 0 45 61 43 720.2 0 43 62 48 720.2 0 48 63 43 720.2 0 43 64 62 720.2 0 62 65 68 720.2 0 68 66 44 720.2 0 44 67 55 720.2 0 55 68 65 720.2 0 65 69 69 720.2 0 69 70 47 720.2 0 47 71 424 720.2 0 424 72 144 720.2 0 144 73 206 720.2 0 206 74 34 720.2 0 34 75 42 720.2 0 42 76 32 720.2 0 32 77 49 720.2 0 49 78 32 720.2 0 32 79 28 720.2 0 28 80 31 720.2 0 31 81 38 720.2 0 38 82 41 720.2 0 41 83 34 720.2 0 34 84 44 720.2 0 44 85 33 720.2 0 33 86 38 720.2 0 38 87 23 720.2 0 23 88 23 720.2 0 23 89 28 720.2 0 28 90 39 720.2 0 39 91 49 720.2 0 49 92 157 720.2 0 157 93 57 720.2 0 57 94 49 720.2 0 49 95 54 720.2 0 54 96 66 720.2 0 66 97 47 720.2 0 47 98 50 720.2 0 50 99 37 720.2 0 37 100 37 720.2 0 37 101 27 720.2 0 27 102 68 720.2 0 68 103 43 720.2 0 43 104 29 720.2 0 29 105 31 720.2 0 31 106 36 720.2 0 36 107 28 720.2 0 28 108 33 720.2 0 33 109 32 720.2 0 32 110 37 720.2 0 37 111 45 720.2 0 45 112 33 720.2 0 33 113 35 720.2 0 35 114 34 720.2 0 34 115 31 720.2 0 31 116 28 720.2 0 28 117 62 720.2 0 62 118 47 720.2 0 47 119 24 720.2 0 24 120 94 720.2 0 94 121 55 720.2 0 55 122 48 720.2 0 48 123 1014 720.2 0 1014 124 70 720.2 0 70 125 28 720.2 0 28 126 59 720.2 0 59 127 36 720.2 0 36 128 61 720.2 0 61 129 78 720.2 0 78 130 1365 720.2 0 1365 131 474 720.2 0 474 132 207 720.2 0 207 133 196 720.2 0 196 134 58 720.2 0 58 135 25 720.2 0 25 136 40 720.2 0 40 137 305 720.2 0 305 138 596 720.2 0 596 139 210 720.2 0 210 140 32 720.2 0 32 141 21 720.2 0 21 142 30 720.2 0 30 143 26 720.2 0 26 144 20 720.2 0 20 145 26 720.2 0 26 146 39 720.2 0 39 147 20 720.2 0 20 148 44 720.2 0 44 149 29 720.2 0 29 150 40 720.2 0 40 151 71 720.2 0 71 152 145 720.2 0 145 153 70 720.2 0 70 154 37 720.2 0 37 155 33 720.2 0 33 156 23 720.2 0 23 157 26 720.2 0 26 158 34 720.2 0 34 159 36 720.2 0 36 160 21 720.2 0 21 161 29 720.2 0 29 162 10 720.2 0 10 163 32 720.2 0 32 164 45 720.2 0 45 165 32 720.2 0 32 166 19 720.2 0 19 167 32 720.2 0 32 168 33 720.2 0 33 169 20 720.2 0 20 170 30 720.2 0 30 171 17 720.2 0 17 172 22 720.2 0 22 173 28 720.2 0 28 174 25 720.2 0 25 175 21 720.2 0 21 176 34 720.2 0 34 177 101 720.2 0 101 178 110 720.2 0 110 179 49 720.2 0 49 180 27 720.2 0 27 181 33 720.2 0 33 182 25 720.2 0 25 183 32 720.2 0 32 184 40 720.2 0 40 185 17 720.2 0 17 186 63 720.2 0 63 187 64 720.2 0 64 188 62 720.2 0 62 189 51 720.2 0 51 190 42 720.2 0 42 191 41 720.2 0 41 192 27 720.2 0 27 193 31 720.2 0 31 194 27 720.2 0 27 195 25 720.2 0 25 196 29 720.2 0 29 197 37 720.2 0 37 198 27 720.2 0 27 199 29 720.2 0 29 200 34 720.2 0 34 201 23 720.2 0 23 202 18 720.2 0 18 203 33 720.2 0 33 204 34 720.2 0 34 205 58 720.2 0 58 206 31 720.2 0 31 207 20 720.2 0 20 208 20 720.2 0 20 209 18 720.2 0 18 210 26 720.2 0 26 211 36 720.2 0 36 212 185 720.2 0 185 213 418 720.2 0 418 214 112 720.2 0 112 215 34 720.2 0 34 216 34 720.2 0 34 217 60 720.2 0 60 218 66 720.2 0 66 219 39 720.2 0 39 220 69 720.2 0 69 221 33 720.2 0 33 222 25 720.2 0 25 223 34 720.2 0 34 224 20 720.2 0 20 225 41 720.2 0 41 226 30 720.2 0 30 227 30 720.2 0 30 228 38 720.2 0 38 229 33 720.2 0 33 230 35 720.2 0 35 231 32 720.2 0 32 232 40 720.2 0 40 233 66 720.2 0 66 234 98 720.2 0 98 235 162 720.2 0 162 236 585 720.2 0 585 237 143 720.2 0 143 238 98 720.2 0 98 239 55 720.2 0 55 240 21 720.2 0 21 241 40 720.2 0 40 242 56 720.2 0 56 243 120 720.2 0 120 244 352 720.2 0 352 245 339 720.2 0 339 246 188 720.2 0 188 247 126 720.2 0 126 248 83 720.2 0 83 249 49 720.2 0 49 250 52 720.2 0 52 251 50 720.2 0 50 252 38 720.2 0 38 253 46 720.2 0 46 254 60 720.2 0 60 255 117 720.2 0 117 256 132 720.2 0 132 257 213 720.2 0 213 258 134 720.2 0 134 259 139 720.2 0 139 260 674 720.2 0 674 261 1384 720.2 0 1384 262 719 720.2 0 719 263 196 720.2 0 196 264 120 720.2 0 120 265 143 720.2 0 143 266 207 720.2 0 207 267 164 720.2 0 164 268 69 720.2 0 69 269 82 720.2 0 82 270 134 720.2 0 134 271 160 720.2 0 160 272 315 720.2 0 315 273 605 720.2 0 605 274 236 720.2 0 236 275 500 720.2 0 500 276 413 720.2 0 413 277 230 720.2 0 230 278 187 720.2 0 187 279 441 720.2 0 441 280 435 720.2 0 435 281 735 720.2 0 735 282 531 720.2 0 531 283 625 720.2 0 625 284 778 720.2 0 778 285 1064 720.2 0 1064 286 23037 720.2 0 23037 287 49829 720.2 0 49829 288 19694 720.2 0 19694 289 92 720.2 0 92 290 40 720.2 0 40 291 52 720.2 0 52 292 73 720.2 0 73 293 40 720.2 0 40 294 38 720.2 0 38 295 192 720.2 0 192 296 75 720.2 0 75 297 176 720.2 0 176 298 225 720.2 0 225 299 221 720.2 0 221 300 47 720.2 0 47 301 30 720.2 0 30 Finished in 14.40 s (75 us/read; 0.80 M reads/minute). === Summary === Total reads processed: 193,198 Reads with adapters: 103,640 (53.6%) Reads that were too short: 31 (0.0%) Reads written (passing filters): 103,609 (53.6%) Total basepairs processed: 55,306,218 bp Total written (filtered): 28,280,067 bp (51.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 103640 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 1 0.0 3 0 0 0 1 18 22 0.0 3 0 0 21 0 1 19 896 0.0 3 0 872 21 2 1 20 101375 0.0 4 99797 1535 37 4 2 21 223 0.0 4 19 202 2 22 17 0.0 4 1 0 15 1 23 47 0.0 4 0 1 0 39 7 24 1 0.0 4 0 0 0 0 1 28 2 0.0 4 0 0 1 1 35 1 0.0 4 0 0 0 0 1 57 1 0.0 4 1 69 2 0.0 4 0 0 0 0 2 98 4 0.0 4 0 0 0 0 4 99 1 0.0 4 0 0 0 0 1 116 1 0.0 4 1 117 2 0.0 4 1 0 0 0 1 125 4 0.0 4 0 0 0 0 4 126 8 0.0 4 0 0 0 0 8 127 1000 0.0 4 0 0 0 0 1000 128 3 0.0 4 0 0 0 0 3 141 1 0.0 4 0 0 0 0 1 146 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 1 153 2 0.0 4 0 0 0 0 2 163 5 0.0 4 0 0 0 0 5 183 1 0.0 4 0 0 0 0 1 222 1 0.0 4 0 0 0 0 1 225 2 0.0 4 1 1 228 5 0.0 4 0 0 0 0 5 229 2 0.0 4 0 0 0 0 2 234 2 0.0 4 0 0 0 0 2 237 1 0.0 4 0 0 0 0 1 256 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 274 1 0.0 4 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 5.53 s (53 us/read; 1.12 M reads/minute). === Summary === Total reads processed: 103,609 Reads with adapters: 1,166 (1.1%) Reads written (passing filters): 103,609 (100.0%) Total basepairs processed: 28,280,067 bp Total written (filtered): 28,263,642 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1166 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 12.8% C: 6.1% G: 78.6% T: 2.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 128 1618.9 0 128 4 13 404.7 0 13 5 5 101.2 0 5 6 1 25.3 0 1 9 10 0.4 0 3 7 10 23 0.1 1 2 21 11 830 0.0 1 7 823 12 101 0.0 1 2 99 13 5 0.0 1 0 5 15 3 0.0 1 0 3 16 3 0.0 1 1 2 20 5 0.0 1 4 1 22 1 0.0 1 1 24 3 0.0 1 3 32 1 0.0 1 0 1 38 1 0.0 1 1 39 1 0.0 1 1 45 3 0.0 1 2 1 60 1 0.0 1 0 1 66 2 0.0 1 2 68 1 0.0 1 1 97 1 0.0 1 1 113 1 0.0 1 1 114 1 0.0 1 1 152 4 0.0 1 0 4 153 1 0.0 1 0 1 159 1 0.0 1 1 163 1 0.0 1 1 171 3 0.0 1 3 191 3 0.0 1 0 3 210 8 0.0 1 1 7 267 1 0.0 1 1