This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 129.30 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 185,007 (6.3%) Reads that were too short: 20,818 (0.7%) Reads written (passing filters): 164,189 (5.6%) Total basepairs processed: 887,907,860 bp Total written (filtered): 45,431,794 bp (5.1%) === Adapter 1 === Sequence: TCGTTA; Type: regular 5'; Length: 6; Trimmed: 185007 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 944 720.2 0 944 7 3350 720.2 0 3350 8 84808 720.2 0 84808 9 52981 720.2 0 52981 10 109 720.2 0 109 11 44 720.2 0 44 12 45 720.2 0 45 13 54 720.2 0 54 14 50 720.2 0 50 15 41 720.2 0 41 16 61 720.2 0 61 17 40 720.2 0 40 18 34 720.2 0 34 19 41 720.2 0 41 20 56 720.2 0 56 21 59 720.2 0 59 22 49 720.2 0 49 23 52 720.2 0 52 24 51 720.2 0 51 25 51 720.2 0 51 26 42 720.2 0 42 27 40 720.2 0 40 28 50 720.2 0 50 29 37 720.2 0 37 30 46 720.2 0 46 31 39 720.2 0 39 32 48 720.2 0 48 33 64 720.2 0 64 34 42 720.2 0 42 35 53 720.2 0 53 36 72 720.2 0 72 37 73 720.2 0 73 38 80 720.2 0 80 39 52 720.2 0 52 40 104 720.2 0 104 41 63 720.2 0 63 42 59 720.2 0 59 43 36 720.2 0 36 44 34 720.2 0 34 45 35 720.2 0 35 46 52 720.2 0 52 47 44 720.2 0 44 48 36 720.2 0 36 49 49 720.2 0 49 50 44 720.2 0 44 51 78 720.2 0 78 52 63 720.2 0 63 53 65 720.2 0 65 54 66 720.2 0 66 55 52 720.2 0 52 56 42 720.2 0 42 57 46 720.2 0 46 58 69 720.2 0 69 59 45 720.2 0 45 60 73 720.2 0 73 61 69 720.2 0 69 62 50 720.2 0 50 63 52 720.2 0 52 64 47 720.2 0 47 65 53 720.2 0 53 66 71 720.2 0 71 67 70 720.2 0 70 68 99 720.2 0 99 69 74 720.2 0 74 70 58 720.2 0 58 71 48 720.2 0 48 72 61 720.2 0 61 73 59 720.2 0 59 74 82 720.2 0 82 75 73 720.2 0 73 76 66 720.2 0 66 77 58 720.2 0 58 78 103 720.2 0 103 79 70 720.2 0 70 80 109 720.2 0 109 81 63 720.2 0 63 82 104 720.2 0 104 83 45 720.2 0 45 84 71 720.2 0 71 85 56 720.2 0 56 86 48 720.2 0 48 87 81 720.2 0 81 88 74 720.2 0 74 89 53 720.2 0 53 90 61 720.2 0 61 91 138 720.2 0 138 92 76 720.2 0 76 93 115 720.2 0 115 94 116 720.2 0 116 95 132 720.2 0 132 96 82 720.2 0 82 97 621 720.2 0 621 98 659 720.2 0 659 99 999 720.2 0 999 100 418 720.2 0 418 101 44 720.2 0 44 102 39 720.2 0 39 103 52 720.2 0 52 104 59 720.2 0 59 105 54 720.2 0 54 106 63 720.2 0 63 107 89 720.2 0 89 108 124 720.2 0 124 109 213 720.2 0 213 110 97 720.2 0 97 111 404 720.2 0 404 112 389 720.2 0 389 113 735 720.2 0 735 114 67 720.2 0 67 115 44 720.2 0 44 116 55 720.2 0 55 117 34 720.2 0 34 118 43 720.2 0 43 119 55 720.2 0 55 120 55 720.2 0 55 121 54 720.2 0 54 122 54 720.2 0 54 123 69 720.2 0 69 124 61 720.2 0 61 125 58 720.2 0 58 126 72 720.2 0 72 127 55 720.2 0 55 128 54 720.2 0 54 129 80 720.2 0 80 130 78 720.2 0 78 131 97 720.2 0 97 132 128 720.2 0 128 133 127 720.2 0 127 134 239 720.2 0 239 135 115 720.2 0 115 136 79 720.2 0 79 137 61 720.2 0 61 138 62 720.2 0 62 139 65 720.2 0 65 140 90 720.2 0 90 141 157 720.2 0 157 142 109 720.2 0 109 143 66 720.2 0 66 144 75 720.2 0 75 145 75 720.2 0 75 146 132 720.2 0 132 147 98 720.2 0 98 148 112 720.2 0 112 149 66 720.2 0 66 150 59 720.2 0 59 151 61 720.2 0 61 152 64 720.2 0 64 153 77 720.2 0 77 154 189 720.2 0 189 155 255 720.2 0 255 156 476 720.2 0 476 157 294 720.2 0 294 158 64 720.2 0 64 159 66 720.2 0 66 160 59 720.2 0 59 161 67 720.2 0 67 162 54 720.2 0 54 163 48 720.2 0 48 164 75 720.2 0 75 165 57 720.2 0 57 166 259 720.2 0 259 167 329 720.2 0 329 168 338 720.2 0 338 169 61 720.2 0 61 170 42 720.2 0 42 171 48 720.2 0 48 172 59 720.2 0 59 173 47 720.2 0 47 174 62 720.2 0 62 175 52 720.2 0 52 176 63 720.2 0 63 177 81 720.2 0 81 178 58 720.2 0 58 179 60 720.2 0 60 180 72 720.2 0 72 181 74 720.2 0 74 182 77 720.2 0 77 183 64 720.2 0 64 184 45 720.2 0 45 185 64 720.2 0 64 186 74 720.2 0 74 187 78 720.2 0 78 188 80 720.2 0 80 189 72 720.2 0 72 190 92 720.2 0 92 191 85 720.2 0 85 192 117 720.2 0 117 193 260 720.2 0 260 194 1482 720.2 0 1482 195 659 720.2 0 659 196 1051 720.2 0 1051 197 128 720.2 0 128 198 163 720.2 0 163 199 100 720.2 0 100 200 158 720.2 0 158 201 170 720.2 0 170 202 138 720.2 0 138 203 58 720.2 0 58 204 66 720.2 0 66 205 77 720.2 0 77 206 81 720.2 0 81 207 76 720.2 0 76 208 87 720.2 0 87 209 73 720.2 0 73 210 77 720.2 0 77 211 74 720.2 0 74 212 66 720.2 0 66 213 66 720.2 0 66 214 69 720.2 0 69 215 119 720.2 0 119 216 141 720.2 0 141 217 130 720.2 0 130 218 77 720.2 0 77 219 74 720.2 0 74 220 314 720.2 0 314 221 440 720.2 0 440 222 485 720.2 0 485 223 331 720.2 0 331 224 69 720.2 0 69 225 66 720.2 0 66 226 70 720.2 0 70 227 64 720.2 0 64 228 80 720.2 0 80 229 108 720.2 0 108 230 63 720.2 0 63 231 48 720.2 0 48 232 58 720.2 0 58 233 74 720.2 0 74 234 53 720.2 0 53 235 68 720.2 0 68 236 95 720.2 0 95 237 246 720.2 0 246 238 372 720.2 0 372 239 379 720.2 0 379 240 139 720.2 0 139 241 185 720.2 0 185 242 241 720.2 0 241 243 422 720.2 0 422 244 258 720.2 0 258 245 364 720.2 0 364 246 210 720.2 0 210 247 80 720.2 0 80 248 70 720.2 0 70 249 111 720.2 0 111 250 96 720.2 0 96 251 160 720.2 0 160 252 219 720.2 0 219 253 173 720.2 0 173 254 386 720.2 0 386 255 456 720.2 0 456 256 721 720.2 0 721 257 121 720.2 0 121 258 139 720.2 0 139 259 174 720.2 0 174 260 126 720.2 0 126 261 133 720.2 0 133 262 1308 720.2 0 1308 263 1276 720.2 0 1276 264 1415 720.2 0 1415 265 170 720.2 0 170 266 64 720.2 0 64 267 72 720.2 0 72 268 71 720.2 0 71 269 150 720.2 0 150 270 143 720.2 0 143 271 189 720.2 0 189 272 96 720.2 0 96 273 134 720.2 0 134 274 95 720.2 0 95 275 173 720.2 0 173 276 155 720.2 0 155 277 84 720.2 0 84 278 79 720.2 0 79 279 111 720.2 0 111 280 199 720.2 0 199 281 171 720.2 0 171 282 224 720.2 0 224 283 153 720.2 0 153 284 111 720.2 0 111 285 128 720.2 0 128 286 86 720.2 0 86 287 94 720.2 0 94 288 132 720.2 0 132 289 171 720.2 0 171 290 204 720.2 0 204 291 187 720.2 0 187 292 149 720.2 0 149 293 665 720.2 0 665 294 1158 720.2 0 1158 295 410 720.2 0 410 296 113 720.2 0 113 297 102 720.2 0 102 298 88 720.2 0 88 299 129 720.2 0 129 300 178 720.2 0 178 301 165 720.2 0 165 Finished in 11.42 s (70 us/read; 0.86 M reads/minute). === Summary === Total reads processed: 164,189 Reads with adapters: 86,505 (52.7%) Reads that were too short: 122 (0.1%) Reads written (passing filters): 86,383 (52.6%) Total basepairs processed: 45,431,794 bp Total written (filtered): 23,824,645 bp (52.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 86505 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 9 0.0 2 0 0 0 9 15 45 0.0 3 0 0 30 15 16 1008 0.0 3 0 899 80 29 17 84225 0.0 3 80640 2982 497 106 18 481 0.0 3 15 183 65 218 19 66 0.0 3 6 3 33 24 20 424 0.0 3 0 0 0 424 21 1 0.0 3 0 1 26 1 0.0 3 1 34 1 0.0 3 0 0 0 1 37 7 0.0 3 1 0 0 6 38 1 0.0 3 0 0 0 1 40 3 0.0 3 0 0 0 3 42 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 45 2 0.0 3 0 0 0 2 46 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 52 3 0.0 3 0 0 0 3 55 4 0.0 3 0 0 0 4 62 1 0.0 3 0 0 0 1 63 2 0.0 3 0 0 0 2 64 2 0.0 3 0 0 0 2 67 1 0.0 3 0 0 0 1 68 2 0.0 3 0 0 0 2 71 1 0.0 3 0 0 0 1 75 9 0.0 3 0 0 0 9 76 18 0.0 3 0 0 1 17 78 3 0.0 3 0 0 0 3 79 3 0.0 3 0 0 0 3 80 3 0.0 3 0 0 0 3 81 2 0.0 3 0 0 0 2 84 2 0.0 3 0 0 0 2 85 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 93 13 0.0 3 0 0 0 13 94 4 0.0 3 0 0 0 4 95 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 97 4 0.0 3 0 0 0 4 99 2 0.0 3 0 0 0 2 100 2 0.0 3 0 0 0 2 102 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 128 3 0.0 3 0 0 0 3 131 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 135 1 0.0 3 0 1 139 2 0.0 3 0 0 0 2 140 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 144 4 0.0 3 0 0 0 4 145 2 0.0 3 0 0 0 2 153 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 1 158 2 0.0 3 0 0 0 2 161 10 0.0 3 0 0 0 10 164 2 0.0 3 0 0 0 2 167 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 183 2 0.0 3 0 0 0 2 184 3 0.0 3 0 0 0 3 186 1 0.0 3 0 0 0 1 187 14 0.0 3 0 0 1 13 188 21 0.0 3 0 0 1 20 191 1 0.0 3 0 0 0 1 192 13 0.0 3 0 0 0 13 193 1 0.0 3 0 0 0 1 194 4 0.0 3 0 0 0 4 196 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 251 1 0.0 3 0 0 0 1 256 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 279 3 0.0 3 0 0 0 3 280 4 0.0 3 0 0 0 4 281 13 0.0 3 0 0 1 12 282 6 0.0 3 0 0 0 6 287 1 0.0 3 0 0 0 1 288 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.73 s (55 us/read; 1.10 M reads/minute). === Summary === Total reads processed: 86,383 Reads with adapters: 334 (0.4%) Reads written (passing filters): 86,383 (100.0%) Total basepairs processed: 23,824,645 bp Total written (filtered): 23,823,608 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 334 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 42.5% C: 17.4% G: 29.0% T: 11.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 306 1349.7 0 306 4 22 337.4 0 22 5 5 84.4 0 5 6 1 21.1 0 1