This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 131.48 s (45 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 175,160 (5.9%) Reads that were too short: 13,576 (0.5%) Reads written (passing filters): 161,584 (5.5%) Total basepairs processed: 887,907,860 bp Total written (filtered): 45,497,713 bp (5.1%) === Adapter 1 === Sequence: TCGTTA; Type: regular 5'; Length: 6; Trimmed: 175160 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 709 720.2 0 709 7 2061 720.2 0 2061 8 64586 720.2 0 64586 9 79912 720.2 0 79912 10 104 720.2 0 104 11 21 720.2 0 21 12 35 720.2 0 35 13 28 720.2 0 28 14 29 720.2 0 29 15 30 720.2 0 30 16 27 720.2 0 27 17 19 720.2 0 19 18 23 720.2 0 23 19 24 720.2 0 24 20 33 720.2 0 33 21 30 720.2 0 30 22 28 720.2 0 28 23 32 720.2 0 32 24 38 720.2 0 38 25 35 720.2 0 35 26 27 720.2 0 27 27 32 720.2 0 32 28 35 720.2 0 35 29 18 720.2 0 18 30 38 720.2 0 38 31 25 720.2 0 25 32 34 720.2 0 34 33 38 720.2 0 38 34 30 720.2 0 30 35 24 720.2 0 24 36 35 720.2 0 35 37 24 720.2 0 24 38 75 720.2 0 75 39 33 720.2 0 33 40 64 720.2 0 64 41 36 720.2 0 36 42 38 720.2 0 38 43 25 720.2 0 25 44 26 720.2 0 26 45 24 720.2 0 24 46 30 720.2 0 30 47 33 720.2 0 33 48 32 720.2 0 32 49 26 720.2 0 26 50 29 720.2 0 29 51 55 720.2 0 55 52 48 720.2 0 48 53 54 720.2 0 54 54 42 720.2 0 42 55 39 720.2 0 39 56 39 720.2 0 39 57 27 720.2 0 27 58 32 720.2 0 32 59 28 720.2 0 28 60 63 720.2 0 63 61 43 720.2 0 43 62 49 720.2 0 49 63 37 720.2 0 37 64 38 720.2 0 38 65 25 720.2 0 25 66 33 720.2 0 33 67 53 720.2 0 53 68 47 720.2 0 47 69 40 720.2 0 40 70 36 720.2 0 36 71 43 720.2 0 43 72 45 720.2 0 45 73 35 720.2 0 35 74 44 720.2 0 44 75 33 720.2 0 33 76 38 720.2 0 38 77 38 720.2 0 38 78 70 720.2 0 70 79 49 720.2 0 49 80 105 720.2 0 105 81 36 720.2 0 36 82 64 720.2 0 64 83 28 720.2 0 28 84 42 720.2 0 42 85 38 720.2 0 38 86 38 720.2 0 38 87 44 720.2 0 44 88 51 720.2 0 51 89 37 720.2 0 37 90 39 720.2 0 39 91 52 720.2 0 52 92 33 720.2 0 33 93 74 720.2 0 74 94 76 720.2 0 76 95 83 720.2 0 83 96 42 720.2 0 42 97 351 720.2 0 351 98 253 720.2 0 253 99 367 720.2 0 367 100 134 720.2 0 134 101 44 720.2 0 44 102 28 720.2 0 28 103 33 720.2 0 33 104 36 720.2 0 36 105 38 720.2 0 38 106 35 720.2 0 35 107 57 720.2 0 57 108 63 720.2 0 63 109 152 720.2 0 152 110 57 720.2 0 57 111 344 720.2 0 344 112 318 720.2 0 318 113 569 720.2 0 569 114 34 720.2 0 34 115 35 720.2 0 35 116 24 720.2 0 24 117 37 720.2 0 37 118 36 720.2 0 36 119 38 720.2 0 38 120 29 720.2 0 29 121 32 720.2 0 32 122 42 720.2 0 42 123 38 720.2 0 38 124 29 720.2 0 29 125 38 720.2 0 38 126 27 720.2 0 27 127 29 720.2 0 29 128 36 720.2 0 36 129 40 720.2 0 40 130 31 720.2 0 31 131 51 720.2 0 51 132 55 720.2 0 55 133 74 720.2 0 74 134 159 720.2 0 159 135 68 720.2 0 68 136 55 720.2 0 55 137 51 720.2 0 51 138 48 720.2 0 48 139 36 720.2 0 36 140 38 720.2 0 38 141 63 720.2 0 63 142 48 720.2 0 48 143 28 720.2 0 28 144 33 720.2 0 33 145 35 720.2 0 35 146 71 720.2 0 71 147 65 720.2 0 65 148 70 720.2 0 70 149 47 720.2 0 47 150 42 720.2 0 42 151 31 720.2 0 31 152 42 720.2 0 42 153 44 720.2 0 44 154 106 720.2 0 106 155 111 720.2 0 111 156 260 720.2 0 260 157 140 720.2 0 140 158 49 720.2 0 49 159 39 720.2 0 39 160 41 720.2 0 41 161 38 720.2 0 38 162 33 720.2 0 33 163 34 720.2 0 34 164 44 720.2 0 44 165 47 720.2 0 47 166 139 720.2 0 139 167 182 720.2 0 182 168 161 720.2 0 161 169 28 720.2 0 28 170 28 720.2 0 28 171 27 720.2 0 27 172 33 720.2 0 33 173 25 720.2 0 25 174 40 720.2 0 40 175 28 720.2 0 28 176 41 720.2 0 41 177 61 720.2 0 61 178 46 720.2 0 46 179 47 720.2 0 47 180 30 720.2 0 30 181 54 720.2 0 54 182 49 720.2 0 49 183 39 720.2 0 39 184 27 720.2 0 27 185 45 720.2 0 45 186 60 720.2 0 60 187 43 720.2 0 43 188 35 720.2 0 35 189 51 720.2 0 51 190 42 720.2 0 42 191 55 720.2 0 55 192 77 720.2 0 77 193 158 720.2 0 158 194 1573 720.2 0 1573 195 479 720.2 0 479 196 1096 720.2 0 1096 197 62 720.2 0 62 198 121 720.2 0 121 199 62 720.2 0 62 200 82 720.2 0 82 201 68 720.2 0 68 202 63 720.2 0 63 203 35 720.2 0 35 204 50 720.2 0 50 205 39 720.2 0 39 206 40 720.2 0 40 207 44 720.2 0 44 208 38 720.2 0 38 209 37 720.2 0 37 210 40 720.2 0 40 211 39 720.2 0 39 212 27 720.2 0 27 213 38 720.2 0 38 214 33 720.2 0 33 215 54 720.2 0 54 216 88 720.2 0 88 217 79 720.2 0 79 218 54 720.2 0 54 219 45 720.2 0 45 220 145 720.2 0 145 221 266 720.2 0 266 222 200 720.2 0 200 223 207 720.2 0 207 224 40 720.2 0 40 225 40 720.2 0 40 226 42 720.2 0 42 227 28 720.2 0 28 228 53 720.2 0 53 229 41 720.2 0 41 230 46 720.2 0 46 231 34 720.2 0 34 232 39 720.2 0 39 233 36 720.2 0 36 234 32 720.2 0 32 235 42 720.2 0 42 236 64 720.2 0 64 237 234 720.2 0 234 238 321 720.2 0 321 239 341 720.2 0 341 240 86 720.2 0 86 241 94 720.2 0 94 242 126 720.2 0 126 243 213 720.2 0 213 244 110 720.2 0 110 245 165 720.2 0 165 246 122 720.2 0 122 247 50 720.2 0 50 248 45 720.2 0 45 249 76 720.2 0 76 250 72 720.2 0 72 251 120 720.2 0 120 252 154 720.2 0 154 253 141 720.2 0 141 254 151 720.2 0 151 255 153 720.2 0 153 256 212 720.2 0 212 257 82 720.2 0 82 258 82 720.2 0 82 259 108 720.2 0 108 260 78 720.2 0 78 261 86 720.2 0 86 262 1048 720.2 0 1048 263 821 720.2 0 821 264 889 720.2 0 889 265 65 720.2 0 65 266 35 720.2 0 35 267 39 720.2 0 39 268 49 720.2 0 49 269 78 720.2 0 78 270 70 720.2 0 70 271 78 720.2 0 78 272 56 720.2 0 56 273 67 720.2 0 67 274 55 720.2 0 55 275 77 720.2 0 77 276 80 720.2 0 80 277 62 720.2 0 62 278 65 720.2 0 65 279 77 720.2 0 77 280 106 720.2 0 106 281 74 720.2 0 74 282 103 720.2 0 103 283 59 720.2 0 59 284 61 720.2 0 61 285 69 720.2 0 69 286 57 720.2 0 57 287 50 720.2 0 50 288 66 720.2 0 66 289 132 720.2 0 132 290 96 720.2 0 96 291 115 720.2 0 115 292 129 720.2 0 129 293 751 720.2 0 751 294 1467 720.2 0 1467 295 455 720.2 0 455 296 69 720.2 0 69 297 52 720.2 0 52 298 38 720.2 0 38 299 77 720.2 0 77 300 138 720.2 0 138 301 81 720.2 0 81 Finished in 12.00 s (74 us/read; 0.81 M reads/minute). === Summary === Total reads processed: 161,584 Reads with adapters: 83,135 (51.5%) Reads that were too short: 27 (0.0%) Reads written (passing filters): 83,108 (51.4%) Total basepairs processed: 45,497,713 bp Total written (filtered): 22,582,243 bp (49.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 83135 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 2 0.0 3 0 0 0 1 1 18 23 0.0 3 0 0 23 19 892 0.0 3 0 879 9 2 2 20 81678 0.0 4 80435 1207 35 1 21 181 0.0 4 28 146 7 22 13 0.0 4 0 1 10 2 23 59 0.0 4 0 0 0 57 2 27 3 0.0 4 0 0 0 0 3 35 1 0.0 4 0 0 0 0 1 39 1 0.0 4 1 74 1 0.0 4 0 0 0 0 1 83 3 0.0 4 0 0 0 0 3 126 3 0.0 4 0 0 0 0 3 127 240 0.0 4 0 0 0 0 240 153 4 0.0 4 0 0 0 0 4 154 2 0.0 4 1 1 178 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 219 1 0.0 4 0 1 222 1 0.0 4 0 1 226 1 0.0 4 1 228 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 232 3 0.0 4 0 0 0 0 3 233 2 0.0 4 0 0 0 0 2 234 3 0.0 4 0 0 0 0 3 235 2 0.0 4 0 0 0 0 2 238 1 0.0 4 0 0 1 255 1 0.0 4 0 0 0 0 1 266 1 0.0 4 0 0 0 1 272 2 0.0 4 0 0 1 0 1 274 1 0.0 4 0 0 1 275 1 0.0 4 0 0 0 0 1 278 1 0.0 4 0 0 0 1 280 1 0.0 4 0 0 0 0 1 284 1 0.0 4 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.44 s (53 us/read; 1.12 M reads/minute). === Summary === Total reads processed: 83,108 Reads with adapters: 6,995 (8.4%) Reads written (passing filters): 83,108 (100.0%) Total basepairs processed: 22,582,243 bp Total written (filtered): 22,496,467 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 6995 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.8% C: 2.9% G: 92.5% T: 1.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 17 1298.6 0 17 4 13 324.6 0 13 5 4 81.2 0 4 6 2 20.3 0 2 7 1 5.1 0 1 9 805 0.3 0 15 790 10 5738 0.1 1 148 5590 11 238 0.0 1 2 236 12 1 0.0 1 0 1 13 4 0.0 1 0 4 18 9 0.0 1 7 2 19 31 0.0 1 20 11 22 36 0.0 1 32 4 23 6 0.0 1 5 1 41 1 0.0 1 1 50 1 0.0 1 1 58 1 0.0 1 1 76 1 0.0 1 1 80 2 0.0 1 1 1 93 1 0.0 1 1 104 2 0.0 1 1 1 105 1 0.0 1 1 120 1 0.0 1 1 126 1 0.0 1 1 127 1 0.0 1 1 150 4 0.0 1 0 4 156 9 0.0 1 9 157 1 0.0 1 1 169 1 0.0 1 1 177 2 0.0 1 2 189 1 0.0 1 1 207 1 0.0 1 0 1 208 42 0.0 1 8 34 209 2 0.0 1 1 1 210 1 0.0 1 1 220 1 0.0 1 1 223 1 0.0 1 1 227 1 0.0 1 1 228 2 0.0 1 2 239 8 0.0 1 8 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.