This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCCTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 135.87 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 326,296 (11.1%) Reads that were too short: 18,640 (0.6%) Reads written (passing filters): 307,656 (10.4%) Total basepairs processed: 887,907,860 bp Total written (filtered): 75,465,807 bp (8.5%) === Adapter 1 === Sequence: AGCCTC; Type: regular 5'; Length: 6; Trimmed: 326296 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2019 720.2 0 2019 7 44836 720.2 0 44836 8 27090 720.2 0 27090 9 106 720.2 0 106 10 185 720.2 0 185 11 95 720.2 0 95 12 89 720.2 0 89 13 85 720.2 0 85 14 80 720.2 0 80 15 74 720.2 0 74 16 72 720.2 0 72 17 77 720.2 0 77 18 86 720.2 0 86 19 161 720.2 0 161 20 110 720.2 0 110 21 104 720.2 0 104 22 129 720.2 0 129 23 87 720.2 0 87 24 99 720.2 0 99 25 95 720.2 0 95 26 170 720.2 0 170 27 64 720.2 0 64 28 162 720.2 0 162 29 327 720.2 0 327 30 88 720.2 0 88 31 58 720.2 0 58 32 57 720.2 0 57 33 83 720.2 0 83 34 85 720.2 0 85 35 54 720.2 0 54 36 127 720.2 0 127 37 93 720.2 0 93 38 178 720.2 0 178 39 455 720.2 0 455 40 174 720.2 0 174 41 246 720.2 0 246 42 129 720.2 0 129 43 217 720.2 0 217 44 362 720.2 0 362 45 124 720.2 0 124 46 110 720.2 0 110 47 114 720.2 0 114 48 146 720.2 0 146 49 299 720.2 0 299 50 343 720.2 0 343 51 629 720.2 0 629 52 1084 720.2 0 1084 53 1195 720.2 0 1195 54 2521 720.2 0 2521 55 3756 720.2 0 3756 56 57345 720.2 0 57345 57 28824 720.2 0 28824 58 97862 720.2 0 97862 59 382 720.2 0 382 60 84 720.2 0 84 61 79 720.2 0 79 62 176 720.2 0 176 63 133 720.2 0 133 64 270 720.2 0 270 65 62 720.2 0 62 66 71 720.2 0 71 67 76 720.2 0 76 68 69 720.2 0 69 69 56 720.2 0 56 70 116 720.2 0 116 71 63 720.2 0 63 72 84 720.2 0 84 73 66 720.2 0 66 74 78 720.2 0 78 75 79 720.2 0 79 76 82 720.2 0 82 77 45 720.2 0 45 78 55 720.2 0 55 79 59 720.2 0 59 80 75 720.2 0 75 81 71 720.2 0 71 82 71 720.2 0 71 83 120 720.2 0 120 84 101 720.2 0 101 85 163 720.2 0 163 86 93 720.2 0 93 87 120 720.2 0 120 88 203 720.2 0 203 89 180 720.2 0 180 90 80 720.2 0 80 91 56 720.2 0 56 92 85 720.2 0 85 93 60 720.2 0 60 94 37 720.2 0 37 95 53 720.2 0 53 96 66 720.2 0 66 97 62 720.2 0 62 98 76 720.2 0 76 99 50 720.2 0 50 100 52 720.2 0 52 101 65 720.2 0 65 102 63 720.2 0 63 103 117 720.2 0 117 104 162 720.2 0 162 105 201 720.2 0 201 106 66 720.2 0 66 107 43 720.2 0 43 108 84 720.2 0 84 109 66 720.2 0 66 110 64 720.2 0 64 111 102 720.2 0 102 112 58 720.2 0 58 113 92 720.2 0 92 114 83 720.2 0 83 115 67 720.2 0 67 116 55 720.2 0 55 117 59 720.2 0 59 118 78 720.2 0 78 119 59 720.2 0 59 120 92 720.2 0 92 121 81 720.2 0 81 122 73 720.2 0 73 123 78 720.2 0 78 124 525 720.2 0 525 125 344 720.2 0 344 126 453 720.2 0 453 127 276 720.2 0 276 128 107 720.2 0 107 129 61 720.2 0 61 130 90 720.2 0 90 131 72 720.2 0 72 132 61 720.2 0 61 133 58 720.2 0 58 134 82 720.2 0 82 135 87 720.2 0 87 136 62 720.2 0 62 137 60 720.2 0 60 138 74 720.2 0 74 139 69 720.2 0 69 140 82 720.2 0 82 141 129 720.2 0 129 142 179 720.2 0 179 143 111 720.2 0 111 144 99 720.2 0 99 145 134 720.2 0 134 146 172 720.2 0 172 147 986 720.2 0 986 148 1318 720.2 0 1318 149 2241 720.2 0 2241 150 743 720.2 0 743 151 222 720.2 0 222 152 529 720.2 0 529 153 118 720.2 0 118 154 91 720.2 0 91 155 113 720.2 0 113 156 75 720.2 0 75 157 63 720.2 0 63 158 96 720.2 0 96 159 96 720.2 0 96 160 261 720.2 0 261 161 204 720.2 0 204 162 325 720.2 0 325 163 88 720.2 0 88 164 99 720.2 0 99 165 128 720.2 0 128 166 191 720.2 0 191 167 1942 720.2 0 1942 168 605 720.2 0 605 169 2260 720.2 0 2260 170 132 720.2 0 132 171 149 720.2 0 149 172 224 720.2 0 224 173 286 720.2 0 286 174 165 720.2 0 165 175 304 720.2 0 304 176 302 720.2 0 302 177 469 720.2 0 469 178 133 720.2 0 133 179 75 720.2 0 75 180 122 720.2 0 122 181 77 720.2 0 77 182 88 720.2 0 88 183 86 720.2 0 86 184 86 720.2 0 86 185 87 720.2 0 87 186 161 720.2 0 161 187 99 720.2 0 99 188 134 720.2 0 134 189 134 720.2 0 134 190 104 720.2 0 104 191 145 720.2 0 145 192 175 720.2 0 175 193 207 720.2 0 207 194 225 720.2 0 225 195 564 720.2 0 564 196 969 720.2 0 969 197 760 720.2 0 760 198 371 720.2 0 371 199 368 720.2 0 368 200 2983 720.2 0 2983 201 2164 720.2 0 2164 202 3965 720.2 0 3965 203 355 720.2 0 355 204 61 720.2 0 61 205 63 720.2 0 63 206 88 720.2 0 88 207 94 720.2 0 94 208 68 720.2 0 68 209 55 720.2 0 55 210 53 720.2 0 53 211 57 720.2 0 57 212 57 720.2 0 57 213 64 720.2 0 64 214 75 720.2 0 75 215 64 720.2 0 64 216 79 720.2 0 79 217 81 720.2 0 81 218 67 720.2 0 67 219 164 720.2 0 164 220 168 720.2 0 168 221 220 720.2 0 220 222 103 720.2 0 103 223 44 720.2 0 44 224 85 720.2 0 85 225 85 720.2 0 85 226 71 720.2 0 71 227 69 720.2 0 69 228 101 720.2 0 101 229 275 720.2 0 275 230 262 720.2 0 262 231 445 720.2 0 445 232 510 720.2 0 510 233 232 720.2 0 232 234 478 720.2 0 478 235 88 720.2 0 88 236 41 720.2 0 41 237 46 720.2 0 46 238 65 720.2 0 65 239 88 720.2 0 88 240 88 720.2 0 88 241 138 720.2 0 138 242 97 720.2 0 97 243 121 720.2 0 121 244 75 720.2 0 75 245 60 720.2 0 60 246 63 720.2 0 63 247 114 720.2 0 114 248 114 720.2 0 114 249 94 720.2 0 94 250 100 720.2 0 100 251 84 720.2 0 84 252 88 720.2 0 88 253 41 720.2 0 41 254 52 720.2 0 52 255 59 720.2 0 59 256 66 720.2 0 66 257 76 720.2 0 76 258 96 720.2 0 96 259 82 720.2 0 82 260 62 720.2 0 62 261 101 720.2 0 101 262 97 720.2 0 97 263 85 720.2 0 85 264 83 720.2 0 83 265 97 720.2 0 97 266 102 720.2 0 102 267 79 720.2 0 79 268 198 720.2 0 198 269 243 720.2 0 243 270 221 720.2 0 221 271 178 720.2 0 178 272 99 720.2 0 99 273 134 720.2 0 134 274 127 720.2 0 127 275 172 720.2 0 172 276 234 720.2 0 234 277 291 720.2 0 291 278 350 720.2 0 350 279 167 720.2 0 167 280 256 720.2 0 256 281 425 720.2 0 425 282 647 720.2 0 647 283 459 720.2 0 459 284 103 720.2 0 103 285 82 720.2 0 82 286 57 720.2 0 57 287 85 720.2 0 85 288 340 720.2 0 340 289 346 720.2 0 346 290 210 720.2 0 210 291 164 720.2 0 164 292 131 720.2 0 131 293 204 720.2 0 204 294 291 720.2 0 291 295 176 720.2 0 176 296 105 720.2 0 105 297 93 720.2 0 93 298 61 720.2 0 61 299 80 720.2 0 80 300 156 720.2 0 156 301 355 720.2 0 355 Finished in 18.81 s (61 us/read; 0.98 M reads/minute). === Summary === Total reads processed: 307,656 Reads with adapters: 46,090 (15.0%) Reads that were too short: 385 (0.1%) Reads written (passing filters): 45,705 (14.9%) Total basepairs processed: 75,465,807 bp Total written (filtered): 12,626,074 bp (16.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 46090 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 19 0.0 3 0 0 11 8 16 351 0.0 3 0 280 49 22 17 44718 0.0 3 42922 1484 249 63 18 112 0.0 3 8 86 10 8 19 42 0.0 3 0 1 24 17 20 263 0.0 3 2 1 2 258 22 4 0.0 3 0 0 0 4 23 7 0.0 3 1 0 0 6 25 2 0.0 3 0 0 0 2 26 3 0.0 3 0 0 0 3 27 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 32 2 0.0 3 0 0 0 2 33 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 36 2 0.0 3 0 0 0 2 44 4 0.0 3 0 0 0 4 45 2 0.0 3 0 0 0 2 46 3 0.0 3 0 0 0 3 47 5 0.0 3 0 0 0 5 48 3 0.0 3 0 0 0 3 49 2 0.0 3 0 0 0 2 50 8 0.0 3 0 0 0 8 53 19 0.0 3 0 0 0 19 55 4 0.0 3 0 0 0 4 56 4 0.0 3 0 0 0 4 57 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 65 7 0.0 3 0 0 0 7 66 1 0.0 3 0 0 0 1 67 6 0.0 3 0 0 0 6 68 4 0.0 3 0 0 0 4 69 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 81 3 0.0 3 0 0 0 3 82 2 0.0 3 0 0 0 2 83 1 0.0 3 0 0 0 1 86 3 0.0 3 0 0 0 3 88 4 0.0 3 0 0 0 4 89 4 0.0 3 0 0 0 4 91 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 98 2 0.0 3 0 0 0 2 100 1 0.0 3 0 0 0 1 101 2 0.0 3 0 0 0 2 102 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 109 2 0.0 3 0 0 0 2 110 1 0.0 3 0 0 0 1 111 3 0.0 3 0 0 0 3 112 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 115 2 0.0 3 0 1 0 1 116 5 0.0 3 0 0 0 5 117 3 0.0 3 0 0 0 3 118 2 0.0 3 0 0 0 2 119 7 0.0 3 0 0 0 7 120 1 0.0 3 0 0 0 1 121 3 0.0 3 1 0 0 2 122 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 125 2 0.0 3 0 0 0 2 126 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 128 3 0.0 3 0 0 0 3 131 1 0.0 3 0 0 0 1 132 2 0.0 3 0 0 0 2 134 1 0.0 3 0 0 0 1 135 2 0.0 3 0 0 0 2 136 10 0.0 3 0 0 0 10 138 3 0.0 3 0 0 0 3 139 5 0.0 3 0 0 0 5 140 2 0.0 3 0 0 0 2 141 2 0.0 3 0 0 0 2 142 8 0.0 3 0 0 0 8 143 2 0.0 3 0 0 0 2 144 2 0.0 3 0 1 0 1 145 97 0.0 3 0 0 6 91 148 2 0.0 3 0 0 0 2 151 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 162 4 0.0 3 0 0 0 4 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 165 6 0.0 3 0 0 1 5 167 3 0.0 3 0 0 0 3 168 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 172 2 0.0 3 0 0 0 2 173 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 183 2 0.0 3 0 0 0 2 184 2 0.0 3 0 0 0 2 187 2 0.0 3 0 0 0 2 188 3 0.0 3 0 0 0 3 190 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 3 0.0 3 0 0 0 3 194 2 0.0 3 1 0 0 1 195 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 219 61 0.0 3 0 0 0 61 220 1 0.0 3 0 0 0 1 221 22 0.0 3 0 0 0 22 222 4 0.0 3 0 0 0 4 223 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 228 3 0.0 3 0 0 0 3 229 59 0.0 3 0 0 0 59 230 12 0.0 3 0 0 0 12 231 9 0.0 3 0 0 1 8 232 13 0.0 3 0 0 0 13 233 4 0.0 3 0 0 0 4 234 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 239 5 0.0 3 0 0 0 5 242 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 246 2 0.0 3 0 0 0 2 247 1 0.0 3 1 253 1 0.0 3 0 0 1 260 1 0.0 3 0 0 0 1 262 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 4 0.0 3 0 0 0 4 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.50 s (55 us/read; 1.10 M reads/minute). === Summary === Total reads processed: 45,705 Reads with adapters: 210 (0.5%) Reads written (passing filters): 45,705 (100.0%) Total basepairs processed: 12,626,074 bp Total written (filtered): 12,625,428 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 210 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 18.1% C: 26.2% G: 46.2% T: 9.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 199 714.1 0 199 4 8 178.5 0 8 5 1 44.6 0 1 6 2 11.2 0 2