This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCAAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 134.20 s (45 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 257,743 (8.7%) Reads that were too short: 17,153 (0.6%) Reads written (passing filters): 240,590 (8.2%) Total basepairs processed: 887,907,860 bp Total written (filtered): 63,609,280 bp (7.2%) === Adapter 1 === Sequence: GTCAAT; Type: regular 5'; Length: 6; Trimmed: 257743 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 989 720.2 0 989 7 51394 720.2 0 51394 8 996 720.2 0 996 9 63906 720.2 0 63906 10 98 720.2 0 98 11 42 720.2 0 42 12 67 720.2 0 67 13 54 720.2 0 54 14 44 720.2 0 44 15 55 720.2 0 55 16 68 720.2 0 68 17 63 720.2 0 63 18 66 720.2 0 66 19 44 720.2 0 44 20 59 720.2 0 59 21 72 720.2 0 72 22 50 720.2 0 50 23 63 720.2 0 63 24 63 720.2 0 63 25 69 720.2 0 69 26 63 720.2 0 63 27 82 720.2 0 82 28 121 720.2 0 121 29 191 720.2 0 191 30 118 720.2 0 118 31 166 720.2 0 166 32 1123 720.2 0 1123 33 148 720.2 0 148 34 94 720.2 0 94 35 123 720.2 0 123 36 107 720.2 0 107 37 149 720.2 0 149 38 282 720.2 0 282 39 250 720.2 0 250 40 316 720.2 0 316 41 399 720.2 0 399 42 7130 720.2 0 7130 43 13288 720.2 0 13288 44 6806 720.2 0 6806 45 93 720.2 0 93 46 63 720.2 0 63 47 97 720.2 0 97 48 226 720.2 0 226 49 67 720.2 0 67 50 123 720.2 0 123 51 119 720.2 0 119 52 120 720.2 0 120 53 196 720.2 0 196 54 125 720.2 0 125 55 132 720.2 0 132 56 59 720.2 0 59 57 110 720.2 0 110 58 145 720.2 0 145 59 186 720.2 0 186 60 291 720.2 0 291 61 493 720.2 0 493 62 516 720.2 0 516 63 1060 720.2 0 1060 64 1485 720.2 0 1485 65 28100 720.2 0 28100 66 11512 720.2 0 11512 67 37475 720.2 0 37475 68 191 720.2 0 191 69 58 720.2 0 58 70 43 720.2 0 43 71 79 720.2 0 79 72 46 720.2 0 46 73 51 720.2 0 51 74 41 720.2 0 41 75 42 720.2 0 42 76 75 720.2 0 75 77 100 720.2 0 100 78 56 720.2 0 56 79 31 720.2 0 31 80 46 720.2 0 46 81 55 720.2 0 55 82 41 720.2 0 41 83 55 720.2 0 55 84 48 720.2 0 48 85 50 720.2 0 50 86 45 720.2 0 45 87 517 720.2 0 517 88 70 720.2 0 70 89 89 720.2 0 89 90 62 720.2 0 62 91 47 720.2 0 47 92 42 720.2 0 42 93 62 720.2 0 62 94 63 720.2 0 63 95 64 720.2 0 64 96 34 720.2 0 34 97 48 720.2 0 48 98 84 720.2 0 84 99 61 720.2 0 61 100 64 720.2 0 64 101 90 720.2 0 90 102 83 720.2 0 83 103 41 720.2 0 41 104 52 720.2 0 52 105 33 720.2 0 33 106 46 720.2 0 46 107 54 720.2 0 54 108 81 720.2 0 81 109 39 720.2 0 39 110 63 720.2 0 63 111 70 720.2 0 70 112 80 720.2 0 80 113 43 720.2 0 43 114 51 720.2 0 51 115 38 720.2 0 38 116 38 720.2 0 38 117 42 720.2 0 42 118 74 720.2 0 74 119 57 720.2 0 57 120 50 720.2 0 50 121 52 720.2 0 52 122 35 720.2 0 35 123 47 720.2 0 47 124 69 720.2 0 69 125 42 720.2 0 42 126 55 720.2 0 55 127 50 720.2 0 50 128 86 720.2 0 86 129 77 720.2 0 77 130 70 720.2 0 70 131 73 720.2 0 73 132 49 720.2 0 49 133 72 720.2 0 72 134 213 720.2 0 213 135 267 720.2 0 267 136 241 720.2 0 241 137 110 720.2 0 110 138 150 720.2 0 150 139 111 720.2 0 111 140 247 720.2 0 247 141 47 720.2 0 47 142 32 720.2 0 32 143 55 720.2 0 55 144 45 720.2 0 45 145 38 720.2 0 38 146 43 720.2 0 43 147 46 720.2 0 46 148 85 720.2 0 85 149 40 720.2 0 40 150 37 720.2 0 37 151 36 720.2 0 36 152 33 720.2 0 33 153 44 720.2 0 44 154 51 720.2 0 51 155 33 720.2 0 33 156 34 720.2 0 34 157 49 720.2 0 49 158 66 720.2 0 66 159 61 720.2 0 61 160 48 720.2 0 48 161 36 720.2 0 36 162 28 720.2 0 28 163 38 720.2 0 38 164 33 720.2 0 33 165 53 720.2 0 53 166 24 720.2 0 24 167 30 720.2 0 30 168 59 720.2 0 59 169 66 720.2 0 66 170 75 720.2 0 75 171 27 720.2 0 27 172 45 720.2 0 45 173 36 720.2 0 36 174 46 720.2 0 46 175 40 720.2 0 40 176 26 720.2 0 26 177 36 720.2 0 36 178 65 720.2 0 65 179 85 720.2 0 85 180 78 720.2 0 78 181 55 720.2 0 55 182 36 720.2 0 36 183 52 720.2 0 52 184 52 720.2 0 52 185 59 720.2 0 59 186 79 720.2 0 79 187 35 720.2 0 35 188 32 720.2 0 32 189 46 720.2 0 46 190 34 720.2 0 34 191 35 720.2 0 35 192 34 720.2 0 34 193 30 720.2 0 30 194 30 720.2 0 30 195 29 720.2 0 29 196 49 720.2 0 49 197 68 720.2 0 68 198 77 720.2 0 77 199 67 720.2 0 67 200 61 720.2 0 61 201 69 720.2 0 69 202 120 720.2 0 120 203 147 720.2 0 147 204 75 720.2 0 75 205 56 720.2 0 56 206 43 720.2 0 43 207 48 720.2 0 48 208 34 720.2 0 34 209 60 720.2 0 60 210 49 720.2 0 49 211 47 720.2 0 47 212 48 720.2 0 48 213 60 720.2 0 60 214 36 720.2 0 36 215 62 720.2 0 62 216 57 720.2 0 57 217 77 720.2 0 77 218 70 720.2 0 70 219 92 720.2 0 92 220 110 720.2 0 110 221 72 720.2 0 72 222 327 720.2 0 327 223 402 720.2 0 402 224 510 720.2 0 510 225 408 720.2 0 408 226 52 720.2 0 52 227 48 720.2 0 48 228 59 720.2 0 59 229 48 720.2 0 48 230 44 720.2 0 44 231 46 720.2 0 46 232 34 720.2 0 34 233 41 720.2 0 41 234 42 720.2 0 42 235 54 720.2 0 54 236 61 720.2 0 61 237 62 720.2 0 62 238 58 720.2 0 58 239 64 720.2 0 64 240 83 720.2 0 83 241 82 720.2 0 82 242 260 720.2 0 260 243 331 720.2 0 331 244 254 720.2 0 254 245 165 720.2 0 165 246 47 720.2 0 47 247 85 720.2 0 85 248 50 720.2 0 50 249 48 720.2 0 48 250 83 720.2 0 83 251 117 720.2 0 117 252 170 720.2 0 170 253 200 720.2 0 200 254 489 720.2 0 489 255 1670 720.2 0 1670 256 811 720.2 0 811 257 1583 720.2 0 1583 258 218 720.2 0 218 259 111 720.2 0 111 260 765 720.2 0 765 261 1344 720.2 0 1344 262 739 720.2 0 739 263 119 720.2 0 119 264 83 720.2 0 83 265 121 720.2 0 121 266 154 720.2 0 154 267 88 720.2 0 88 268 51 720.2 0 51 269 63 720.2 0 63 270 314 720.2 0 314 271 427 720.2 0 427 272 351 720.2 0 351 273 68 720.2 0 68 274 73 720.2 0 73 275 39 720.2 0 39 276 69 720.2 0 69 277 138 720.2 0 138 278 151 720.2 0 151 279 118 720.2 0 118 280 55 720.2 0 55 281 41 720.2 0 41 282 43 720.2 0 43 283 47 720.2 0 47 284 52 720.2 0 52 285 33 720.2 0 33 286 37 720.2 0 37 287 49 720.2 0 49 288 42 720.2 0 42 289 38 720.2 0 38 290 57 720.2 0 57 291 83 720.2 0 83 292 93 720.2 0 93 293 81 720.2 0 81 294 89 720.2 0 89 295 39 720.2 0 39 296 51 720.2 0 51 297 62 720.2 0 62 298 51 720.2 0 51 299 77 720.2 0 77 300 96 720.2 0 96 301 82 720.2 0 82 Finished in 15.90 s (66 us/read; 0.91 M reads/minute). === Summary === Total reads processed: 240,590 Reads with adapters: 65,767 (27.3%) Reads that were too short: 189 (0.1%) Reads written (passing filters): 65,578 (27.3%) Total basepairs processed: 63,609,280 bp Total written (filtered): 18,021,795 bp (28.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 65767 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 8 0.0 2 0 0 0 8 15 43 0.0 3 0 0 29 14 16 721 0.0 3 0 648 61 12 17 64231 0.0 3 60936 2811 406 78 18 183 0.0 3 3 138 16 26 19 100 0.0 3 0 0 55 45 20 199 0.0 3 2 0 0 197 34 1 0.0 3 0 0 0 1 55 3 0.0 3 0 0 0 3 57 5 0.0 3 0 0 0 5 58 3 0.0 3 0 0 0 3 59 1 0.0 3 0 0 0 1 60 3 0.0 3 0 0 0 3 61 5 0.0 3 0 0 0 5 64 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 70 2 0.0 3 0 0 0 2 71 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 81 2 0.0 3 0 0 0 2 82 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 91 2 0.0 3 0 0 0 2 92 2 0.0 3 0 0 0 2 98 1 0.0 3 0 0 0 1 101 3 0.0 3 0 0 0 3 104 1 0.0 3 0 0 1 106 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 108 3 0.0 3 0 0 0 3 121 1 0.0 3 0 0 0 1 122 1 0.0 3 1 124 1 0.0 3 0 0 0 1 126 1 0.0 3 1 128 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 143 5 0.0 3 0 0 0 5 144 16 0.0 3 0 0 0 16 146 2 0.0 3 0 0 0 2 147 5 0.0 3 0 0 0 5 149 3 0.0 3 0 0 0 3 150 4 0.0 3 0 0 0 4 153 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 1 159 5 0.0 3 0 0 0 5 164 1 0.0 3 0 0 0 1 165 2 0.0 3 0 0 0 2 167 11 0.0 3 0 0 0 11 168 4 0.0 3 0 0 0 4 174 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 1 191 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 193 5 0.0 3 0 0 0 5 194 11 0.0 3 0 0 2 9 195 4 0.0 3 0 1 0 3 200 3 0.0 3 0 0 0 3 201 1 0.0 3 0 0 0 1 204 5 0.0 3 0 0 0 5 205 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 221 3 0.0 3 0 0 0 3 222 1 0.0 3 0 0 0 1 223 4 0.0 3 0 0 0 4 224 2 0.0 3 0 0 0 2 225 1 0.0 3 0 0 0 1 226 2 0.0 3 0 0 0 2 230 2 0.0 3 0 0 0 2 232 1 0.0 3 0 0 0 1 233 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 242 1 0.0 3 0 0 0 1 246 2 0.0 3 0 0 0 2 250 1 0.0 3 0 0 0 1 253 1 0.0 3 0 0 0 1 254 1 0.0 3 0 0 0 1 261 5 0.0 3 0 0 0 5 262 78 0.0 3 0 0 6 72 263 1 0.0 3 0 0 0 1 264 1 0.0 3 0 0 0 1 266 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 280 2 0.0 3 0 0 0 2 281 7 0.0 3 0 0 0 7 282 4 0.0 3 0 0 0 4 283 2 0.0 3 0 0 0 2 284 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.58 s (55 us/read; 1.10 M reads/minute). === Summary === Total reads processed: 65,578 Reads with adapters: 1,663 (2.5%) Reads written (passing filters): 65,578 (100.0%) Total basepairs processed: 18,021,795 bp Total written (filtered): 18,016,672 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1663 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 50.5% C: 16.0% G: 31.5% T: 2.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1536 1024.7 0 1536 4 123 256.2 0 123 5 1 64.0 0 1 6 3 16.0 0 3