This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCAAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 133.78 s (45 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 280,147 (9.5%) Reads that were too short: 13,010 (0.4%) Reads written (passing filters): 267,137 (9.1%) Total basepairs processed: 887,907,860 bp Total written (filtered): 70,039,984 bp (7.9%) === Adapter 1 === Sequence: GTCAAT; Type: regular 5'; Length: 6; Trimmed: 280147 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1022 720.2 0 1022 7 66843 720.2 0 66843 8 659 720.2 0 659 9 53006 720.2 0 53006 10 93 720.2 0 93 11 78 720.2 0 78 12 88 720.2 0 88 13 94 720.2 0 94 14 57 720.2 0 57 15 76 720.2 0 76 16 120 720.2 0 120 17 100 720.2 0 100 18 74 720.2 0 74 19 89 720.2 0 89 20 110 720.2 0 110 21 95 720.2 0 95 22 96 720.2 0 96 23 119 720.2 0 119 24 78 720.2 0 78 25 108 720.2 0 108 26 119 720.2 0 119 27 128 720.2 0 128 28 177 720.2 0 177 29 221 720.2 0 221 30 161 720.2 0 161 31 198 720.2 0 198 32 1233 720.2 0 1233 33 167 720.2 0 167 34 126 720.2 0 126 35 172 720.2 0 172 36 137 720.2 0 137 37 141 720.2 0 141 38 303 720.2 0 303 39 241 720.2 0 241 40 312 720.2 0 312 41 306 720.2 0 306 42 6804 720.2 0 6804 43 12845 720.2 0 12845 44 6558 720.2 0 6558 45 136 720.2 0 136 46 102 720.2 0 102 47 121 720.2 0 121 48 280 720.2 0 280 49 90 720.2 0 90 50 183 720.2 0 183 51 164 720.2 0 164 52 200 720.2 0 200 53 300 720.2 0 300 54 151 720.2 0 151 55 177 720.2 0 177 56 115 720.2 0 115 57 189 720.2 0 189 58 189 720.2 0 189 59 196 720.2 0 196 60 340 720.2 0 340 61 549 720.2 0 549 62 612 720.2 0 612 63 1256 720.2 0 1256 64 1668 720.2 0 1668 65 34928 720.2 0 34928 66 14247 720.2 0 14247 67 47055 720.2 0 47055 68 248 720.2 0 248 69 85 720.2 0 85 70 65 720.2 0 65 71 77 720.2 0 77 72 64 720.2 0 64 73 83 720.2 0 83 74 82 720.2 0 82 75 67 720.2 0 67 76 79 720.2 0 79 77 97 720.2 0 97 78 57 720.2 0 57 79 89 720.2 0 89 80 88 720.2 0 88 81 84 720.2 0 84 82 90 720.2 0 90 83 92 720.2 0 92 84 73 720.2 0 73 85 82 720.2 0 82 86 65 720.2 0 65 87 470 720.2 0 470 88 89 720.2 0 89 89 120 720.2 0 120 90 50 720.2 0 50 91 80 720.2 0 80 92 76 720.2 0 76 93 68 720.2 0 68 94 102 720.2 0 102 95 85 720.2 0 85 96 82 720.2 0 82 97 79 720.2 0 79 98 101 720.2 0 101 99 83 720.2 0 83 100 78 720.2 0 78 101 94 720.2 0 94 102 100 720.2 0 100 103 74 720.2 0 74 104 78 720.2 0 78 105 90 720.2 0 90 106 90 720.2 0 90 107 86 720.2 0 86 108 137 720.2 0 137 109 86 720.2 0 86 110 96 720.2 0 96 111 94 720.2 0 94 112 68 720.2 0 68 113 72 720.2 0 72 114 56 720.2 0 56 115 69 720.2 0 69 116 72 720.2 0 72 117 60 720.2 0 60 118 82 720.2 0 82 119 69 720.2 0 69 120 64 720.2 0 64 121 81 720.2 0 81 122 54 720.2 0 54 123 57 720.2 0 57 124 70 720.2 0 70 125 59 720.2 0 59 126 69 720.2 0 69 127 92 720.2 0 92 128 79 720.2 0 79 129 123 720.2 0 123 130 104 720.2 0 104 131 100 720.2 0 100 132 78 720.2 0 78 133 81 720.2 0 81 134 119 720.2 0 119 135 149 720.2 0 149 136 121 720.2 0 121 137 81 720.2 0 81 138 126 720.2 0 126 139 117 720.2 0 117 140 250 720.2 0 250 141 57 720.2 0 57 142 56 720.2 0 56 143 79 720.2 0 79 144 56 720.2 0 56 145 51 720.2 0 51 146 61 720.2 0 61 147 58 720.2 0 58 148 60 720.2 0 60 149 54 720.2 0 54 150 46 720.2 0 46 151 50 720.2 0 50 152 56 720.2 0 56 153 54 720.2 0 54 154 58 720.2 0 58 155 45 720.2 0 45 156 50 720.2 0 50 157 53 720.2 0 53 158 76 720.2 0 76 159 88 720.2 0 88 160 66 720.2 0 66 161 69 720.2 0 69 162 56 720.2 0 56 163 49 720.2 0 49 164 42 720.2 0 42 165 48 720.2 0 48 166 43 720.2 0 43 167 53 720.2 0 53 168 89 720.2 0 89 169 81 720.2 0 81 170 99 720.2 0 99 171 57 720.2 0 57 172 65 720.2 0 65 173 58 720.2 0 58 174 68 720.2 0 68 175 67 720.2 0 67 176 46 720.2 0 46 177 37 720.2 0 37 178 89 720.2 0 89 179 75 720.2 0 75 180 71 720.2 0 71 181 66 720.2 0 66 182 54 720.2 0 54 183 56 720.2 0 56 184 48 720.2 0 48 185 71 720.2 0 71 186 79 720.2 0 79 187 56 720.2 0 56 188 60 720.2 0 60 189 53 720.2 0 53 190 52 720.2 0 52 191 84 720.2 0 84 192 41 720.2 0 41 193 35 720.2 0 35 194 53 720.2 0 53 195 71 720.2 0 71 196 51 720.2 0 51 197 79 720.2 0 79 198 54 720.2 0 54 199 48 720.2 0 48 200 61 720.2 0 61 201 50 720.2 0 50 202 71 720.2 0 71 203 84 720.2 0 84 204 59 720.2 0 59 205 63 720.2 0 63 206 56 720.2 0 56 207 62 720.2 0 62 208 54 720.2 0 54 209 76 720.2 0 76 210 74 720.2 0 74 211 53 720.2 0 53 212 54 720.2 0 54 213 57 720.2 0 57 214 48 720.2 0 48 215 62 720.2 0 62 216 76 720.2 0 76 217 91 720.2 0 91 218 59 720.2 0 59 219 68 720.2 0 68 220 79 720.2 0 79 221 75 720.2 0 75 222 151 720.2 0 151 223 204 720.2 0 204 224 189 720.2 0 189 225 150 720.2 0 150 226 59 720.2 0 59 227 61 720.2 0 61 228 75 720.2 0 75 229 42 720.2 0 42 230 46 720.2 0 46 231 48 720.2 0 48 232 52 720.2 0 52 233 50 720.2 0 50 234 51 720.2 0 51 235 65 720.2 0 65 236 89 720.2 0 89 237 51 720.2 0 51 238 59 720.2 0 59 239 56 720.2 0 56 240 79 720.2 0 79 241 76 720.2 0 76 242 167 720.2 0 167 243 201 720.2 0 201 244 148 720.2 0 148 245 165 720.2 0 165 246 85 720.2 0 85 247 96 720.2 0 96 248 77 720.2 0 77 249 70 720.2 0 70 250 92 720.2 0 92 251 85 720.2 0 85 252 137 720.2 0 137 253 124 720.2 0 124 254 243 720.2 0 243 255 980 720.2 0 980 256 437 720.2 0 437 257 1010 720.2 0 1010 258 132 720.2 0 132 259 94 720.2 0 94 260 630 720.2 0 630 261 1364 720.2 0 1364 262 658 720.2 0 658 263 111 720.2 0 111 264 81 720.2 0 81 265 122 720.2 0 122 266 122 720.2 0 122 267 72 720.2 0 72 268 53 720.2 0 53 269 42 720.2 0 42 270 196 720.2 0 196 271 185 720.2 0 185 272 159 720.2 0 159 273 61 720.2 0 61 274 58 720.2 0 58 275 52 720.2 0 52 276 69 720.2 0 69 277 75 720.2 0 75 278 80 720.2 0 80 279 90 720.2 0 90 280 72 720.2 0 72 281 67 720.2 0 67 282 56 720.2 0 56 283 56 720.2 0 56 284 52 720.2 0 52 285 46 720.2 0 46 286 48 720.2 0 48 287 44 720.2 0 44 288 54 720.2 0 54 289 46 720.2 0 46 290 54 720.2 0 54 291 86 720.2 0 86 292 93 720.2 0 93 293 72 720.2 0 72 294 86 720.2 0 86 295 41 720.2 0 41 296 52 720.2 0 52 297 55 720.2 0 55 298 73 720.2 0 73 299 88 720.2 0 88 300 89 720.2 0 89 301 83 720.2 0 83 Finished in 18.48 s (69 us/read; 0.87 M reads/minute). === Summary === Total reads processed: 267,137 Reads with adapters: 69,334 (26.0%) Reads that were too short: 60 (0.0%) Reads written (passing filters): 69,274 (25.9%) Total basepairs processed: 70,039,984 bp Total written (filtered): 18,739,011 bp (26.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 69334 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 21 0.0 3 0 0 21 19 717 0.0 3 0 689 24 3 1 20 66137 0.0 4 65008 1100 25 1 3 21 126 0.0 4 23 99 2 1 1 22 6 0.0 4 0 0 3 1 2 23 51 0.0 4 0 1 0 38 12 24 3 0.0 4 1 0 0 1 1 25 1 0.0 4 0 0 0 0 1 26 2 0.0 4 1 0 0 1 27 2 0.0 4 0 0 0 0 2 35 1 0.0 4 0 0 0 0 1 47 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 0 1 74 2 0.0 4 0 0 0 0 2 83 2 0.0 4 0 0 0 0 2 91 1 0.0 4 0 0 0 0 1 92 17 0.0 4 0 0 0 0 17 107 42 0.0 4 0 0 0 0 42 113 24 0.0 4 0 0 0 0 24 114 1 0.0 4 0 0 0 1 115 3 0.0 4 0 0 0 0 3 117 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 125 4 0.0 4 0 0 0 0 4 126 24 0.0 4 0 0 0 0 24 127 2073 0.0 4 0 0 0 0 2073 128 8 0.0 4 0 0 0 0 8 129 2 0.0 4 0 0 0 0 2 130 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 1 155 1 0.0 4 0 0 0 0 1 167 1 0.0 4 0 0 0 0 1 168 1 0.0 4 0 0 0 0 1 185 1 0.0 4 0 0 0 0 1 193 25 0.0 4 0 0 0 1 24 194 13 0.0 4 0 0 0 0 13 195 4 0.0 4 1 0 0 0 3 196 1 0.0 4 1 203 1 0.0 4 0 0 0 0 1 220 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 229 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 237 2 0.0 4 0 1 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.64 s (53 us/read; 1.14 M reads/minute). === Summary === Total reads processed: 69,274 Reads with adapters: 907 (1.3%) Reads written (passing filters): 69,274 (100.0%) Total basepairs processed: 18,739,011 bp Total written (filtered): 18,719,914 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 907 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 17.6% C: 7.5% G: 71.1% T: 3.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 111 1082.4 0 111 4 12 270.6 0 12 5 2 67.7 0 2 6 2 16.9 0 2 9 5 0.3 0 2 3 10 10 0.1 1 0 10 11 553 0.0 1 2 551 12 99 0.0 1 4 95 15 4 0.0 1 0 4 20 12 0.0 1 9 3 21 10 0.0 1 8 2 23 1 0.0 1 0 1 24 34 0.0 1 31 3 25 1 0.0 1 1 29 1 0.0 1 1 33 1 0.0 1 0 1 41 1 0.0 1 1 78 1 0.0 1 1 79 2 0.0 1 2 108 1 0.0 1 1 111 1 0.0 1 0 1 133 1 0.0 1 1 152 7 0.0 1 0 7 171 1 0.0 1 1 179 1 0.0 1 0 1 210 4 0.0 1 2 2 225 16 0.0 1 16 226 1 0.0 1 1 259 1 0.0 1 1 261 10 0.0 1 8 2 264 1 0.0 1 1