This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTATGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 136.27 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 186,287 (6.3%) Reads that were too short: 21,197 (0.7%) Reads written (passing filters): 165,090 (5.6%) Total basepairs processed: 887,907,860 bp Total written (filtered): 43,737,084 bp (4.9%) === Adapter 1 === Sequence: GTATGT; Type: regular 5'; Length: 6; Trimmed: 186287 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3039 720.2 0 3039 7 1263 720.2 0 1263 8 69971 720.2 0 69971 9 54025 720.2 0 54025 10 90 720.2 0 90 11 56 720.2 0 56 12 52 720.2 0 52 13 53 720.2 0 53 14 53 720.2 0 53 15 47 720.2 0 47 16 44 720.2 0 44 17 56 720.2 0 56 18 54 720.2 0 54 19 52 720.2 0 52 20 45 720.2 0 45 21 41 720.2 0 41 22 52 720.2 0 52 23 37 720.2 0 37 24 196 720.2 0 196 25 80 720.2 0 80 26 74 720.2 0 74 27 62 720.2 0 62 28 52 720.2 0 52 29 58 720.2 0 58 30 51 720.2 0 51 31 64 720.2 0 64 32 34 720.2 0 34 33 39 720.2 0 39 34 64 720.2 0 64 35 59 720.2 0 59 36 109 720.2 0 109 37 46 720.2 0 46 38 63 720.2 0 63 39 57 720.2 0 57 40 49 720.2 0 49 41 30 720.2 0 30 42 42 720.2 0 42 43 67 720.2 0 67 44 46 720.2 0 46 45 40 720.2 0 40 46 59 720.2 0 59 47 39 720.2 0 39 48 42 720.2 0 42 49 52 720.2 0 52 50 49 720.2 0 49 51 55 720.2 0 55 52 52 720.2 0 52 53 50 720.2 0 50 54 54 720.2 0 54 55 56 720.2 0 56 56 37 720.2 0 37 57 49 720.2 0 49 58 52 720.2 0 52 59 35 720.2 0 35 60 43 720.2 0 43 61 41 720.2 0 41 62 34 720.2 0 34 63 48 720.2 0 48 64 53 720.2 0 53 65 84 720.2 0 84 66 53 720.2 0 53 67 127 720.2 0 127 68 56 720.2 0 56 69 120 720.2 0 120 70 102 720.2 0 102 71 125 720.2 0 125 72 74 720.2 0 74 73 62 720.2 0 62 74 47 720.2 0 47 75 65 720.2 0 65 76 117 720.2 0 117 77 121 720.2 0 121 78 214 720.2 0 214 79 111 720.2 0 111 80 594 720.2 0 594 81 433 720.2 0 433 82 1126 720.2 0 1126 83 95 720.2 0 95 84 63 720.2 0 63 85 60 720.2 0 60 86 89 720.2 0 89 87 73 720.2 0 73 88 64 720.2 0 64 89 62 720.2 0 62 90 65 720.2 0 65 91 75 720.2 0 75 92 67 720.2 0 67 93 97 720.2 0 97 94 107 720.2 0 107 95 122 720.2 0 122 96 1580 720.2 0 1580 97 1464 720.2 0 1464 98 1746 720.2 0 1746 99 109 720.2 0 109 100 132 720.2 0 132 101 51 720.2 0 51 102 57 720.2 0 57 103 41 720.2 0 41 104 55 720.2 0 55 105 42 720.2 0 42 106 41 720.2 0 41 107 55 720.2 0 55 108 57 720.2 0 57 109 50 720.2 0 50 110 77 720.2 0 77 111 50 720.2 0 50 112 70 720.2 0 70 113 93 720.2 0 93 114 152 720.2 0 152 115 68 720.2 0 68 116 69 720.2 0 69 117 58 720.2 0 58 118 49 720.2 0 49 119 56 720.2 0 56 120 82 720.2 0 82 121 55 720.2 0 55 122 85 720.2 0 85 123 65 720.2 0 65 124 54 720.2 0 54 125 67 720.2 0 67 126 116 720.2 0 116 127 160 720.2 0 160 128 184 720.2 0 184 129 200 720.2 0 200 130 484 720.2 0 484 131 1040 720.2 0 1040 132 566 720.2 0 566 133 689 720.2 0 689 134 515 720.2 0 515 135 172 720.2 0 172 136 89 720.2 0 89 137 133 720.2 0 133 138 361 720.2 0 361 139 137 720.2 0 137 140 81 720.2 0 81 141 73 720.2 0 73 142 66 720.2 0 66 143 73 720.2 0 73 144 88 720.2 0 88 145 88 720.2 0 88 146 85 720.2 0 85 147 139 720.2 0 139 148 1102 720.2 0 1102 149 2098 720.2 0 2098 150 818 720.2 0 818 151 47 720.2 0 47 152 43 720.2 0 43 153 71 720.2 0 71 154 74 720.2 0 74 155 64 720.2 0 64 156 65 720.2 0 65 157 61 720.2 0 61 158 90 720.2 0 90 159 87 720.2 0 87 160 69 720.2 0 69 161 75 720.2 0 75 162 65 720.2 0 65 163 51 720.2 0 51 164 60 720.2 0 60 165 62 720.2 0 62 166 59 720.2 0 59 167 49 720.2 0 49 168 45 720.2 0 45 169 61 720.2 0 61 170 63 720.2 0 63 171 51 720.2 0 51 172 54 720.2 0 54 173 68 720.2 0 68 174 90 720.2 0 90 175 85 720.2 0 85 176 78 720.2 0 78 177 84 720.2 0 84 178 73 720.2 0 73 179 73 720.2 0 73 180 82 720.2 0 82 181 82 720.2 0 82 182 84 720.2 0 84 183 90 720.2 0 90 184 125 720.2 0 125 185 178 720.2 0 178 186 224 720.2 0 224 187 1355 720.2 0 1355 188 5002 720.2 0 5002 189 2083 720.2 0 2083 190 1004 720.2 0 1004 191 74 720.2 0 74 192 83 720.2 0 83 193 29 720.2 0 29 194 61 720.2 0 61 195 96 720.2 0 96 196 81 720.2 0 81 197 53 720.2 0 53 198 70 720.2 0 70 199 52 720.2 0 52 200 52 720.2 0 52 201 44 720.2 0 44 202 48 720.2 0 48 203 76 720.2 0 76 204 64 720.2 0 64 205 90 720.2 0 90 206 86 720.2 0 86 207 104 720.2 0 104 208 107 720.2 0 107 209 537 720.2 0 537 210 990 720.2 0 990 211 906 720.2 0 906 212 242 720.2 0 242 213 66 720.2 0 66 214 75 720.2 0 75 215 66 720.2 0 66 216 47 720.2 0 47 217 64 720.2 0 64 218 62 720.2 0 62 219 72 720.2 0 72 220 58 720.2 0 58 221 79 720.2 0 79 222 63 720.2 0 63 223 107 720.2 0 107 224 117 720.2 0 117 225 112 720.2 0 112 226 410 720.2 0 410 227 677 720.2 0 677 228 361 720.2 0 361 229 201 720.2 0 201 230 165 720.2 0 165 231 292 720.2 0 292 232 162 720.2 0 162 233 91 720.2 0 91 234 183 720.2 0 183 235 186 720.2 0 186 236 162 720.2 0 162 237 132 720.2 0 132 238 88 720.2 0 88 239 101 720.2 0 101 240 287 720.2 0 287 241 480 720.2 0 480 242 467 720.2 0 467 243 393 720.2 0 393 244 269 720.2 0 269 245 208 720.2 0 208 246 444 720.2 0 444 247 165 720.2 0 165 248 107 720.2 0 107 249 224 720.2 0 224 250 226 720.2 0 226 251 240 720.2 0 240 252 188 720.2 0 188 253 561 720.2 0 561 254 983 720.2 0 983 255 858 720.2 0 858 256 361 720.2 0 361 257 227 720.2 0 227 258 126 720.2 0 126 259 116 720.2 0 116 260 367 720.2 0 367 261 377 720.2 0 377 262 406 720.2 0 406 263 109 720.2 0 109 264 74 720.2 0 74 265 131 720.2 0 131 266 254 720.2 0 254 267 277 720.2 0 277 268 158 720.2 0 158 269 177 720.2 0 177 270 618 720.2 0 618 271 413 720.2 0 413 272 453 720.2 0 453 273 428 720.2 0 428 274 431 720.2 0 431 275 236 720.2 0 236 276 57 720.2 0 57 277 39 720.2 0 39 278 43 720.2 0 43 279 89 720.2 0 89 280 64 720.2 0 64 281 96 720.2 0 96 282 68 720.2 0 68 283 135 720.2 0 135 284 143 720.2 0 143 285 108 720.2 0 108 286 60 720.2 0 60 287 54 720.2 0 54 288 48 720.2 0 48 289 52 720.2 0 52 290 60 720.2 0 60 291 46 720.2 0 46 292 64 720.2 0 64 293 73 720.2 0 73 294 78 720.2 0 78 295 75 720.2 0 75 296 78 720.2 0 78 297 55 720.2 0 55 298 80 720.2 0 80 299 73 720.2 0 73 300 60 720.2 0 60 301 111 720.2 0 111 Finished in 11.60 s (70 us/read; 0.85 M reads/minute). === Summary === Total reads processed: 165,090 Reads with adapters: 72,134 (43.7%) Reads that were too short: 88 (0.1%) Reads written (passing filters): 72,046 (43.6%) Total basepairs processed: 43,737,084 bp Total written (filtered): 19,864,512 bp (45.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 72134 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 13 0.0 2 0 0 0 13 15 40 0.0 3 0 0 26 14 16 819 0.0 3 0 742 58 19 17 68368 0.0 3 64992 2860 421 95 18 207 0.0 3 9 154 21 23 19 151 0.0 3 17 10 84 40 20 2297 0.0 3 1942 118 36 201 21 7 0.0 3 0 7 22 1 0.0 3 0 0 0 1 23 9 0.0 3 1 0 0 8 26 1 0.0 3 0 0 0 1 30 1 0.0 3 1 34 9 0.0 3 1 0 0 8 37 3 0.0 3 0 0 0 3 38 3 0.0 3 0 0 0 3 42 1 0.0 3 0 0 0 1 44 5 0.0 3 0 0 0 5 47 1 0.0 3 0 0 0 1 51 3 0.0 3 0 0 0 3 58 1 0.0 3 0 0 0 1 59 20 0.0 3 0 0 0 20 61 3 0.0 3 0 0 0 3 62 2 0.0 3 0 0 0 2 63 2 0.0 3 0 0 0 2 65 3 0.0 3 0 0 0 3 68 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 70 49 0.0 3 0 0 0 49 72 1 0.0 3 0 0 0 1 73 4 0.0 3 0 0 1 3 76 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 1 82 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 1 111 2 0.0 3 0 0 0 2 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 116 2 0.0 3 0 0 0 2 121 2 0.0 3 0 0 0 2 127 2 0.0 3 0 0 0 2 130 4 0.0 3 0 0 0 4 140 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 160 3 0.0 3 0 0 0 3 163 2 0.0 3 0 0 0 2 164 2 0.0 3 0 1 0 1 169 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 171 2 0.0 3 0 0 0 2 172 2 0.0 3 0 0 0 2 174 2 0.0 3 0 0 0 2 183 2 0.0 3 0 0 0 2 184 1 0.0 3 0 0 0 1 187 1 0.0 3 0 1 191 1 0.0 3 0 0 0 1 193 2 0.0 3 0 0 0 2 194 18 0.0 3 0 0 2 16 195 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 1 206 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 2 0.0 3 0 0 0 2 221 4 0.0 3 0 0 0 4 226 1 0.0 3 0 0 0 1 238 2 0.0 3 0 0 0 2 269 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 3 0.0 3 0 0 0 3 280 3 0.0 3 0 0 0 3 281 7 0.0 3 0 0 0 7 282 6 0.0 3 0 0 0 6 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.04 s (56 us/read; 1.07 M reads/minute). === Summary === Total reads processed: 72,046 Reads with adapters: 874 (1.2%) Reads written (passing filters): 72,046 (100.0%) Total basepairs processed: 19,864,512 bp Total written (filtered): 19,861,765 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 874 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 62.0% C: 12.0% G: 22.7% T: 3.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 757 1125.7 0 757 4 110 281.4 0 110 5 6 70.4 0 6 6 1 17.6 0 1