This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTTGCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 134.15 s (45 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 56,288 (1.9%) Reads that were too short: 11,977 (0.4%) Reads written (passing filters): 44,311 (1.5%) Total basepairs processed: 887,907,860 bp Total written (filtered): 11,536,686 bp (1.3%) === Adapter 1 === Sequence: GTTGCC; Type: regular 5'; Length: 6; Trimmed: 56288 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 601 720.2 0 601 7 16815 720.2 0 16815 8 13369 720.2 0 13369 9 223 720.2 0 223 10 330 720.2 0 330 11 27 720.2 0 27 12 41 720.2 0 41 13 40 720.2 0 40 14 36 720.2 0 36 15 27 720.2 0 27 16 28 720.2 0 28 17 15 720.2 0 15 18 18 720.2 0 18 19 32 720.2 0 32 20 29 720.2 0 29 21 48 720.2 0 48 22 33 720.2 0 33 23 21 720.2 0 21 24 29 720.2 0 29 25 27 720.2 0 27 26 11 720.2 0 11 27 21 720.2 0 21 28 20 720.2 0 20 29 16 720.2 0 16 30 30 720.2 0 30 31 19 720.2 0 19 32 29 720.2 0 29 33 32 720.2 0 32 34 32 720.2 0 32 35 25 720.2 0 25 36 29 720.2 0 29 37 38 720.2 0 38 38 117 720.2 0 117 39 34 720.2 0 34 40 55 720.2 0 55 41 35 720.2 0 35 42 29 720.2 0 29 43 32 720.2 0 32 44 25 720.2 0 25 45 20 720.2 0 20 46 41 720.2 0 41 47 23 720.2 0 23 48 160 720.2 0 160 49 77 720.2 0 77 50 27 720.2 0 27 51 23 720.2 0 23 52 49 720.2 0 49 53 39 720.2 0 39 54 16 720.2 0 16 55 36 720.2 0 36 56 18 720.2 0 18 57 22 720.2 0 22 58 36 720.2 0 36 59 30 720.2 0 30 60 48 720.2 0 48 61 53 720.2 0 53 62 29 720.2 0 29 63 23 720.2 0 23 64 33 720.2 0 33 65 30 720.2 0 30 66 34 720.2 0 34 67 30 720.2 0 30 68 35 720.2 0 35 69 247 720.2 0 247 70 140 720.2 0 140 71 406 720.2 0 406 72 166 720.2 0 166 73 92 720.2 0 92 74 324 720.2 0 324 75 31 720.2 0 31 76 29 720.2 0 29 77 36 720.2 0 36 78 20 720.2 0 20 79 49 720.2 0 49 80 50 720.2 0 50 81 47 720.2 0 47 82 84 720.2 0 84 83 136 720.2 0 136 84 367 720.2 0 367 85 256 720.2 0 256 86 314 720.2 0 314 87 29 720.2 0 29 88 37 720.2 0 37 89 33 720.2 0 33 90 75 720.2 0 75 91 76 720.2 0 76 92 29 720.2 0 29 93 25 720.2 0 25 94 49 720.2 0 49 95 25 720.2 0 25 96 27 720.2 0 27 97 32 720.2 0 32 98 30 720.2 0 30 99 44 720.2 0 44 100 31 720.2 0 31 101 42 720.2 0 42 102 40 720.2 0 40 103 27 720.2 0 27 104 24 720.2 0 24 105 26 720.2 0 26 106 26 720.2 0 26 107 28 720.2 0 28 108 38 720.2 0 38 109 27 720.2 0 27 110 97 720.2 0 97 111 499 720.2 0 499 112 158 720.2 0 158 113 42 720.2 0 42 114 30 720.2 0 30 115 22 720.2 0 22 116 22 720.2 0 22 117 28 720.2 0 28 118 14 720.2 0 14 119 25 720.2 0 25 120 33 720.2 0 33 121 29 720.2 0 29 122 27 720.2 0 27 123 32 720.2 0 32 124 44 720.2 0 44 125 39 720.2 0 39 126 26 720.2 0 26 127 21 720.2 0 21 128 36 720.2 0 36 129 33 720.2 0 33 130 29 720.2 0 29 131 133 720.2 0 133 132 60 720.2 0 60 133 39 720.2 0 39 134 32 720.2 0 32 135 30 720.2 0 30 136 43 720.2 0 43 137 48 720.2 0 48 138 39 720.2 0 39 139 32 720.2 0 32 140 26 720.2 0 26 141 30 720.2 0 30 142 47 720.2 0 47 143 46 720.2 0 46 144 40 720.2 0 40 145 82 720.2 0 82 146 103 720.2 0 103 147 163 720.2 0 163 148 126 720.2 0 126 149 158 720.2 0 158 150 161 720.2 0 161 151 91 720.2 0 91 152 60 720.2 0 60 153 60 720.2 0 60 154 32 720.2 0 32 155 46 720.2 0 46 156 59 720.2 0 59 157 96 720.2 0 96 158 99 720.2 0 99 159 53 720.2 0 53 160 30 720.2 0 30 161 49 720.2 0 49 162 29 720.2 0 29 163 36 720.2 0 36 164 53 720.2 0 53 165 30 720.2 0 30 166 29 720.2 0 29 167 37 720.2 0 37 168 135 720.2 0 135 169 76 720.2 0 76 170 103 720.2 0 103 171 59 720.2 0 59 172 27 720.2 0 27 173 37 720.2 0 37 174 31 720.2 0 31 175 40 720.2 0 40 176 245 720.2 0 245 177 175 720.2 0 175 178 419 720.2 0 419 179 36 720.2 0 36 180 20 720.2 0 20 181 33 720.2 0 33 182 101 720.2 0 101 183 45 720.2 0 45 184 203 720.2 0 203 185 32 720.2 0 32 186 51 720.2 0 51 187 113 720.2 0 113 188 139 720.2 0 139 189 97 720.2 0 97 190 86 720.2 0 86 191 45 720.2 0 45 192 50 720.2 0 50 193 33 720.2 0 33 194 58 720.2 0 58 195 96 720.2 0 96 196 108 720.2 0 108 197 222 720.2 0 222 198 439 720.2 0 439 199 255 720.2 0 255 200 82 720.2 0 82 201 43 720.2 0 43 202 139 720.2 0 139 203 396 720.2 0 396 204 394 720.2 0 394 205 345 720.2 0 345 206 196 720.2 0 196 207 46 720.2 0 46 208 42 720.2 0 42 209 59 720.2 0 59 210 80 720.2 0 80 211 74 720.2 0 74 212 118 720.2 0 118 213 211 720.2 0 211 214 225 720.2 0 225 215 180 720.2 0 180 216 73 720.2 0 73 217 61 720.2 0 61 218 83 720.2 0 83 219 106 720.2 0 106 220 49 720.2 0 49 221 40 720.2 0 40 222 158 720.2 0 158 223 671 720.2 0 671 224 1082 720.2 0 1082 225 476 720.2 0 476 226 42 720.2 0 42 227 24 720.2 0 24 228 48 720.2 0 48 229 37 720.2 0 37 230 44 720.2 0 44 231 29 720.2 0 29 232 26 720.2 0 26 233 37 720.2 0 37 234 41 720.2 0 41 235 55 720.2 0 55 236 46 720.2 0 46 237 62 720.2 0 62 238 64 720.2 0 64 239 65 720.2 0 65 240 91 720.2 0 91 241 60 720.2 0 60 242 86 720.2 0 86 243 66 720.2 0 66 244 91 720.2 0 91 245 119 720.2 0 119 246 90 720.2 0 90 247 68 720.2 0 68 248 55 720.2 0 55 249 51 720.2 0 51 250 47 720.2 0 47 251 62 720.2 0 62 252 35 720.2 0 35 253 38 720.2 0 38 254 46 720.2 0 46 255 47 720.2 0 47 256 44 720.2 0 44 257 70 720.2 0 70 258 198 720.2 0 198 259 318 720.2 0 318 260 279 720.2 0 279 261 89 720.2 0 89 262 63 720.2 0 63 263 73 720.2 0 73 264 39 720.2 0 39 265 34 720.2 0 34 266 36 720.2 0 36 267 26 720.2 0 26 268 45 720.2 0 45 269 51 720.2 0 51 270 66 720.2 0 66 271 174 720.2 0 174 272 151 720.2 0 151 273 153 720.2 0 153 274 48 720.2 0 48 275 47 720.2 0 47 276 63 720.2 0 63 277 55 720.2 0 55 278 86 720.2 0 86 279 60 720.2 0 60 280 41 720.2 0 41 281 69 720.2 0 69 282 131 720.2 0 131 283 132 720.2 0 132 284 178 720.2 0 178 285 136 720.2 0 136 286 118 720.2 0 118 287 133 720.2 0 133 288 89 720.2 0 89 289 48 720.2 0 48 290 48 720.2 0 48 291 55 720.2 0 55 292 66 720.2 0 66 293 121 720.2 0 121 294 61 720.2 0 61 295 55 720.2 0 55 296 90 720.2 0 90 297 207 720.2 0 207 298 294 720.2 0 294 299 403 720.2 0 403 300 161 720.2 0 161 301 97 720.2 0 97 Finished in 3.14 s (71 us/read; 0.85 M reads/minute). === Summary === Total reads processed: 44,311 Reads with adapters: 16,730 (37.8%) Reads that were too short: 54 (0.1%) Reads written (passing filters): 16,676 (37.6%) Total basepairs processed: 11,536,686 bp Total written (filtered): 4,607,245 bp (39.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 16730 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 3 0.0 3 0 0 1 2 16 158 0.0 3 0 131 19 8 17 16286 0.0 3 15372 771 112 31 18 48 0.0 3 10 35 3 19 30 0.0 3 0 1 17 12 20 72 0.0 3 1 1 0 70 25 2 0.0 3 0 0 0 2 29 2 0.0 3 0 0 0 2 32 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 1 40 2 0.0 3 0 0 0 2 41 21 0.0 3 0 0 0 21 42 1 0.0 3 0 0 0 1 44 3 0.0 3 0 0 0 3 47 1 0.0 3 0 0 0 1 57 2 0.0 3 0 0 0 2 58 4 0.0 3 1 1 0 2 60 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 1 78 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 81 11 0.0 3 0 0 0 11 82 1 0.0 3 0 0 0 1 84 2 0.0 3 0 0 0 2 86 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 94 4 0.0 3 0 0 0 4 95 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 110 2 0.0 3 0 0 0 2 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 120 4 0.0 3 0 0 0 4 124 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 133 2 0.0 3 0 0 0 2 137 3 0.0 3 0 0 0 3 140 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 163 2 0.0 3 0 0 0 2 164 2 0.0 3 0 0 0 2 169 1 0.0 3 0 0 0 1 182 2 0.0 3 0 0 0 2 183 2 0.0 3 0 0 0 2 185 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 255 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 281 2 0.0 3 0 1 0 1 283 1 0.0 3 0 0 0 1 284 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.97 s (58 us/read; 1.03 M reads/minute). === Summary === Total reads processed: 16,676 Reads with adapters: 82 (0.5%) Reads written (passing filters): 16,676 (100.0%) Total basepairs processed: 4,607,245 bp Total written (filtered): 4,606,991 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 82 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 11.0% C: 65.9% G: 19.5% T: 3.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 78 260.6 0 78 5 4 16.3 0 4