This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTTGCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 135.78 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 49,195 (1.7%) Reads that were too short: 6,828 (0.2%) Reads written (passing filters): 42,367 (1.4%) Total basepairs processed: 887,907,860 bp Total written (filtered): 11,366,473 bp (1.3%) === Adapter 1 === Sequence: GTTGCC; Type: regular 5'; Length: 6; Trimmed: 49195 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 621 720.2 0 621 7 14454 720.2 0 14454 8 16904 720.2 0 16904 9 87 720.2 0 87 10 69 720.2 0 69 11 39 720.2 0 39 12 26 720.2 0 26 13 19 720.2 0 19 14 49 720.2 0 49 15 34 720.2 0 34 16 34 720.2 0 34 17 28 720.2 0 28 18 19 720.2 0 19 19 27 720.2 0 27 20 27 720.2 0 27 21 29 720.2 0 29 22 23 720.2 0 23 23 23 720.2 0 23 24 29 720.2 0 29 25 14 720.2 0 14 26 16 720.2 0 16 27 18 720.2 0 18 28 26 720.2 0 26 29 30 720.2 0 30 30 29 720.2 0 29 31 21 720.2 0 21 32 27 720.2 0 27 33 15 720.2 0 15 34 24 720.2 0 24 35 12 720.2 0 12 36 25 720.2 0 25 37 28 720.2 0 28 38 34 720.2 0 34 39 24 720.2 0 24 40 37 720.2 0 37 41 29 720.2 0 29 42 20 720.2 0 20 43 40 720.2 0 40 44 23 720.2 0 23 45 17 720.2 0 17 46 17 720.2 0 17 47 30 720.2 0 30 48 29 720.2 0 29 49 43 720.2 0 43 50 23 720.2 0 23 51 24 720.2 0 24 52 42 720.2 0 42 53 25 720.2 0 25 54 28 720.2 0 28 55 33 720.2 0 33 56 33 720.2 0 33 57 25 720.2 0 25 58 30 720.2 0 30 59 32 720.2 0 32 60 39 720.2 0 39 61 31 720.2 0 31 62 23 720.2 0 23 63 32 720.2 0 32 64 22 720.2 0 22 65 31 720.2 0 31 66 26 720.2 0 26 67 49 720.2 0 49 68 31 720.2 0 31 69 307 720.2 0 307 70 171 720.2 0 171 71 496 720.2 0 496 72 161 720.2 0 161 73 88 720.2 0 88 74 398 720.2 0 398 75 54 720.2 0 54 76 28 720.2 0 28 77 29 720.2 0 29 78 24 720.2 0 24 79 27 720.2 0 27 80 40 720.2 0 40 81 54 720.2 0 54 82 47 720.2 0 47 83 103 720.2 0 103 84 341 720.2 0 341 85 255 720.2 0 255 86 370 720.2 0 370 87 21 720.2 0 21 88 40 720.2 0 40 89 24 720.2 0 24 90 26 720.2 0 26 91 47 720.2 0 47 92 26 720.2 0 26 93 17 720.2 0 17 94 48 720.2 0 48 95 23 720.2 0 23 96 21 720.2 0 21 97 30 720.2 0 30 98 26 720.2 0 26 99 46 720.2 0 46 100 24 720.2 0 24 101 26 720.2 0 26 102 38 720.2 0 38 103 13 720.2 0 13 104 22 720.2 0 22 105 30 720.2 0 30 106 33 720.2 0 33 107 30 720.2 0 30 108 30 720.2 0 30 109 27 720.2 0 27 110 67 720.2 0 67 111 408 720.2 0 408 112 119 720.2 0 119 113 28 720.2 0 28 114 29 720.2 0 29 115 14 720.2 0 14 116 32 720.2 0 32 117 26 720.2 0 26 118 23 720.2 0 23 119 27 720.2 0 27 120 31 720.2 0 31 121 25 720.2 0 25 122 17 720.2 0 17 123 21 720.2 0 21 124 31 720.2 0 31 125 29 720.2 0 29 126 31 720.2 0 31 127 21 720.2 0 21 128 37 720.2 0 37 129 21 720.2 0 21 130 20 720.2 0 20 131 87 720.2 0 87 132 30 720.2 0 30 133 23 720.2 0 23 134 35 720.2 0 35 135 30 720.2 0 30 136 21 720.2 0 21 137 42 720.2 0 42 138 38 720.2 0 38 139 31 720.2 0 31 140 28 720.2 0 28 141 24 720.2 0 24 142 32 720.2 0 32 143 25 720.2 0 25 144 32 720.2 0 32 145 41 720.2 0 41 146 64 720.2 0 64 147 57 720.2 0 57 148 94 720.2 0 94 149 138 720.2 0 138 150 181 720.2 0 181 151 40 720.2 0 40 152 22 720.2 0 22 153 40 720.2 0 40 154 30 720.2 0 30 155 25 720.2 0 25 156 39 720.2 0 39 157 65 720.2 0 65 158 77 720.2 0 77 159 48 720.2 0 48 160 24 720.2 0 24 161 45 720.2 0 45 162 27 720.2 0 27 163 18 720.2 0 18 164 22 720.2 0 22 165 12 720.2 0 12 166 19 720.2 0 19 167 31 720.2 0 31 168 129 720.2 0 129 169 40 720.2 0 40 170 103 720.2 0 103 171 32 720.2 0 32 172 32 720.2 0 32 173 21 720.2 0 21 174 30 720.2 0 30 175 31 720.2 0 31 176 85 720.2 0 85 177 99 720.2 0 99 178 188 720.2 0 188 179 25 720.2 0 25 180 34 720.2 0 34 181 28 720.2 0 28 182 102 720.2 0 102 183 28 720.2 0 28 184 269 720.2 0 269 185 30 720.2 0 30 186 27 720.2 0 27 187 40 720.2 0 40 188 49 720.2 0 49 189 68 720.2 0 68 190 49 720.2 0 49 191 31 720.2 0 31 192 35 720.2 0 35 193 20 720.2 0 20 194 35 720.2 0 35 195 39 720.2 0 39 196 62 720.2 0 62 197 81 720.2 0 81 198 201 720.2 0 201 199 155 720.2 0 155 200 38 720.2 0 38 201 28 720.2 0 28 202 111 720.2 0 111 203 338 720.2 0 338 204 149 720.2 0 149 205 193 720.2 0 193 206 68 720.2 0 68 207 43 720.2 0 43 208 30 720.2 0 30 209 40 720.2 0 40 210 49 720.2 0 49 211 46 720.2 0 46 212 36 720.2 0 36 213 62 720.2 0 62 214 70 720.2 0 70 215 53 720.2 0 53 216 28 720.2 0 28 217 44 720.2 0 44 218 49 720.2 0 49 219 57 720.2 0 57 220 26 720.2 0 26 221 28 720.2 0 28 222 73 720.2 0 73 223 323 720.2 0 323 224 666 720.2 0 666 225 306 720.2 0 306 226 28 720.2 0 28 227 23 720.2 0 23 228 26 720.2 0 26 229 26 720.2 0 26 230 49 720.2 0 49 231 28 720.2 0 28 232 27 720.2 0 27 233 36 720.2 0 36 234 26 720.2 0 26 235 23 720.2 0 23 236 29 720.2 0 29 237 40 720.2 0 40 238 45 720.2 0 45 239 40 720.2 0 40 240 48 720.2 0 48 241 37 720.2 0 37 242 32 720.2 0 32 243 28 720.2 0 28 244 39 720.2 0 39 245 53 720.2 0 53 246 30 720.2 0 30 247 61 720.2 0 61 248 43 720.2 0 43 249 36 720.2 0 36 250 41 720.2 0 41 251 49 720.2 0 49 252 28 720.2 0 28 253 35 720.2 0 35 254 42 720.2 0 42 255 27 720.2 0 27 256 25 720.2 0 25 257 36 720.2 0 36 258 96 720.2 0 96 259 146 720.2 0 146 260 198 720.2 0 198 261 65 720.2 0 65 262 34 720.2 0 34 263 35 720.2 0 35 264 31 720.2 0 31 265 25 720.2 0 25 266 25 720.2 0 25 267 26 720.2 0 26 268 33 720.2 0 33 269 25 720.2 0 25 270 40 720.2 0 40 271 88 720.2 0 88 272 98 720.2 0 98 273 79 720.2 0 79 274 29 720.2 0 29 275 30 720.2 0 30 276 45 720.2 0 45 277 36 720.2 0 36 278 38 720.2 0 38 279 32 720.2 0 32 280 28 720.2 0 28 281 39 720.2 0 39 282 55 720.2 0 55 283 77 720.2 0 77 284 96 720.2 0 96 285 66 720.2 0 66 286 56 720.2 0 56 287 53 720.2 0 53 288 39 720.2 0 39 289 50 720.2 0 50 290 39 720.2 0 39 291 35 720.2 0 35 292 34 720.2 0 34 293 179 720.2 0 179 294 43 720.2 0 43 295 38 720.2 0 38 296 37 720.2 0 37 297 98 720.2 0 98 298 161 720.2 0 161 299 200 720.2 0 200 300 87 720.2 0 87 301 40 720.2 0 40 Finished in 3.26 s (77 us/read; 0.78 M reads/minute). === Summary === Total reads processed: 42,367 Reads with adapters: 17,806 (42.0%) Reads that were too short: 8 (0.0%) Reads written (passing filters): 17,798 (42.0%) Total basepairs processed: 11,366,473 bp Total written (filtered): 4,838,613 bp (42.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 17806 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 2 18 12 0.0 3 0 0 12 19 263 0.0 3 0 260 2 1 20 17259 0.0 4 16960 288 11 21 41 0.0 4 14 25 2 22 6 0.0 4 0 0 6 23 14 0.0 4 0 0 0 13 1 24 1 0.0 4 1 38 1 0.0 4 0 1 39 1 0.0 4 0 0 0 0 1 47 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 106 1 0.0 4 1 108 9 0.0 4 0 0 0 0 9 125 3 0.0 4 0 0 0 0 3 127 183 0.0 4 0 0 0 0 183 140 1 0.0 4 0 0 0 0 1 186 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 244 1 0.0 4 1 256 1 0.0 4 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.97 s (55 us/read; 1.10 M reads/minute). === Summary === Total reads processed: 17,798 Reads with adapters: 1,445 (8.1%) Reads written (passing filters): 17,798 (100.0%) Total basepairs processed: 4,838,613 bp Total written (filtered): 4,815,441 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1445 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 32.0% C: 2.3% G: 64.1% T: 1.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 15 278.1 0 15 4 6 69.5 0 6 9 44 0.1 0 1 43 10 89 0.0 1 3 86 11 792 0.0 1 4 788 12 2 0.0 1 0 2 14 1 0.0 1 0 1 18 1 0.0 1 0 1 19 420 0.0 1 339 81 20 21 0.0 1 18 3 21 9 0.0 1 8 1 22 1 0.0 1 1 23 9 0.0 1 8 1 34 1 0.0 1 1 106 1 0.0 1 0 1 114 28 0.0 1 26 2 115 1 0.0 1 1 159 2 0.0 1 2 205 1 0.0 1 1 261 1 0.0 1 1