This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TACTTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 70.06 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 213,968 (8.0%) Reads that were too short: 30,477 (1.1%) Reads written (passing filters): 183,491 (6.9%) Total basepairs processed: 800,111,879 bp Total written (filtered): 46,649,376 bp (5.8%) === Adapter 1 === Sequence: TACTTC; Type: regular 5'; Length: 6; Trimmed: 213968 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 8566 649.0 0 8566 7 61528 649.0 0 61528 8 736 649.0 0 736 9 59414 649.0 0 59414 10 43 649.0 0 43 11 27 649.0 0 27 12 37 649.0 0 37 13 12 649.0 0 12 14 20 649.0 0 20 15 14 649.0 0 14 16 30 649.0 0 30 17 36 649.0 0 36 18 33 649.0 0 33 19 32 649.0 0 32 20 21 649.0 0 21 21 25 649.0 0 25 22 27 649.0 0 27 23 26 649.0 0 26 24 18 649.0 0 18 25 32 649.0 0 32 26 32 649.0 0 32 27 31 649.0 0 31 28 29 649.0 0 29 29 21 649.0 0 21 30 21 649.0 0 21 31 47 649.0 0 47 32 20 649.0 0 20 33 22 649.0 0 22 34 24 649.0 0 24 35 23 649.0 0 23 36 23 649.0 0 23 37 25 649.0 0 25 38 32 649.0 0 32 39 35 649.0 0 35 40 43 649.0 0 43 41 27 649.0 0 27 42 26 649.0 0 26 43 38 649.0 0 38 44 33 649.0 0 33 45 98 649.0 0 98 46 40 649.0 0 40 47 37 649.0 0 37 48 25 649.0 0 25 49 29 649.0 0 29 50 37 649.0 0 37 51 35 649.0 0 35 52 28 649.0 0 28 53 26 649.0 0 26 54 24 649.0 0 24 55 22 649.0 0 22 56 71 649.0 0 71 57 82 649.0 0 82 58 73 649.0 0 73 59 44 649.0 0 44 60 37 649.0 0 37 61 29 649.0 0 29 62 37 649.0 0 37 63 42 649.0 0 42 64 33 649.0 0 33 65 28 649.0 0 28 66 38 649.0 0 38 67 50 649.0 0 50 68 39 649.0 0 39 69 30 649.0 0 30 70 31 649.0 0 31 71 39 649.0 0 39 72 46 649.0 0 46 73 67 649.0 0 67 74 24 649.0 0 24 75 30 649.0 0 30 76 32 649.0 0 32 77 36 649.0 0 36 78 39 649.0 0 39 79 38 649.0 0 38 80 31 649.0 0 31 81 49 649.0 0 49 82 60 649.0 0 60 83 76 649.0 0 76 84 50 649.0 0 50 85 82 649.0 0 82 86 109 649.0 0 109 87 364 649.0 0 364 88 175 649.0 0 175 89 256 649.0 0 256 90 160 649.0 0 160 91 241 649.0 0 241 92 58 649.0 0 58 93 52 649.0 0 52 94 129 649.0 0 129 95 89 649.0 0 89 96 38 649.0 0 38 97 81 649.0 0 81 98 72 649.0 0 72 99 52 649.0 0 52 100 68 649.0 0 68 101 87 649.0 0 87 102 120 649.0 0 120 103 344 649.0 0 344 104 346 649.0 0 346 105 324 649.0 0 324 106 161 649.0 0 161 107 104 649.0 0 104 108 87 649.0 0 87 109 87 649.0 0 87 110 47 649.0 0 47 111 55 649.0 0 55 112 53 649.0 0 53 113 74 649.0 0 74 114 69 649.0 0 69 115 56 649.0 0 56 116 65 649.0 0 65 117 46 649.0 0 46 118 58 649.0 0 58 119 58 649.0 0 58 120 76 649.0 0 76 121 105 649.0 0 105 122 99 649.0 0 99 123 88 649.0 0 88 124 54 649.0 0 54 125 102 649.0 0 102 126 128 649.0 0 128 127 134 649.0 0 134 128 113 649.0 0 113 129 125 649.0 0 125 130 140 649.0 0 140 131 120 649.0 0 120 132 149 649.0 0 149 133 210 649.0 0 210 134 154 649.0 0 154 135 180 649.0 0 180 136 99 649.0 0 99 137 104 649.0 0 104 138 138 649.0 0 138 139 132 649.0 0 132 140 391 649.0 0 391 141 240 649.0 0 240 142 304 649.0 0 304 143 269 649.0 0 269 144 261 649.0 0 261 145 376 649.0 0 376 146 3799 649.0 0 3799 147 4932 649.0 0 4932 148 4645 649.0 0 4645 149 575 649.0 0 575 150 6876 649.0 0 6876 151 8688 649.0 0 8688 152 6571 649.0 0 6571 153 83 649.0 0 83 154 87 649.0 0 87 155 107 649.0 0 107 156 795 649.0 0 795 157 310 649.0 0 310 158 358 649.0 0 358 159 114 649.0 0 114 160 147 649.0 0 147 161 216 649.0 0 216 162 139 649.0 0 139 163 65 649.0 0 65 164 61 649.0 0 61 165 75 649.0 0 75 166 80 649.0 0 80 167 73 649.0 0 73 168 84 649.0 0 84 169 79 649.0 0 79 170 64 649.0 0 64 171 60 649.0 0 60 172 37 649.0 0 37 173 68 649.0 0 68 174 66 649.0 0 66 175 283 649.0 0 283 176 117 649.0 0 117 177 216 649.0 0 216 178 67 649.0 0 67 179 58 649.0 0 58 180 61 649.0 0 61 181 158 649.0 0 158 182 127 649.0 0 127 183 112 649.0 0 112 184 99 649.0 0 99 185 105 649.0 0 105 186 163 649.0 0 163 187 127 649.0 0 127 188 94 649.0 0 94 189 68 649.0 0 68 190 62 649.0 0 62 191 57 649.0 0 57 192 84 649.0 0 84 193 71 649.0 0 71 194 80 649.0 0 80 195 78 649.0 0 78 196 74 649.0 0 74 197 70 649.0 0 70 198 74 649.0 0 74 199 81 649.0 0 81 200 91 649.0 0 91 201 91 649.0 0 91 202 115 649.0 0 115 203 166 649.0 0 166 204 144 649.0 0 144 205 105 649.0 0 105 206 176 649.0 0 176 207 132 649.0 0 132 208 249 649.0 0 249 209 154 649.0 0 154 210 96 649.0 0 96 211 144 649.0 0 144 212 417 649.0 0 417 213 182 649.0 0 182 214 177 649.0 0 177 215 116 649.0 0 116 216 98 649.0 0 98 217 63 649.0 0 63 218 75 649.0 0 75 219 98 649.0 0 98 220 246 649.0 0 246 221 189 649.0 0 189 222 223 649.0 0 223 223 122 649.0 0 122 224 109 649.0 0 109 225 139 649.0 0 139 226 159 649.0 0 159 227 119 649.0 0 119 228 85 649.0 0 85 229 94 649.0 0 94 230 96 649.0 0 96 231 121 649.0 0 121 232 109 649.0 0 109 233 122 649.0 0 122 234 224 649.0 0 224 235 143 649.0 0 143 236 123 649.0 0 123 237 153 649.0 0 153 238 116 649.0 0 116 239 126 649.0 0 126 240 113 649.0 0 113 241 134 649.0 0 134 242 99 649.0 0 99 243 151 649.0 0 151 244 115 649.0 0 115 245 116 649.0 0 116 246 121 649.0 0 121 247 101 649.0 0 101 248 126 649.0 0 126 249 140 649.0 0 140 250 113 649.0 0 113 251 164 649.0 0 164 252 173 649.0 0 173 253 212 649.0 0 212 254 336 649.0 0 336 255 1298 649.0 0 1298 256 4060 649.0 0 4060 257 5106 649.0 0 5106 258 4269 649.0 0 4269 259 1532 649.0 0 1532 260 186 649.0 0 186 261 165 649.0 0 165 262 251 649.0 0 251 263 261 649.0 0 261 264 160 649.0 0 160 265 119 649.0 0 119 266 97 649.0 0 97 267 136 649.0 0 136 268 192 649.0 0 192 269 128 649.0 0 128 270 85 649.0 0 85 271 87 649.0 0 87 272 94 649.0 0 94 273 104 649.0 0 104 274 102 649.0 0 102 275 125 649.0 0 125 276 87 649.0 0 87 277 94 649.0 0 94 278 103 649.0 0 103 279 173 649.0 0 173 280 396 649.0 0 396 281 410 649.0 0 410 282 328 649.0 0 328 283 209 649.0 0 209 284 169 649.0 0 169 285 184 649.0 0 184 286 133 649.0 0 133 287 115 649.0 0 115 288 165 649.0 0 165 289 156 649.0 0 156 290 109 649.0 0 109 291 65 649.0 0 65 292 92 649.0 0 92 293 141 649.0 0 141 294 178 649.0 0 178 295 137 649.0 0 137 296 185 649.0 0 185 297 131 649.0 0 131 298 119 649.0 0 119 299 95 649.0 0 95 300 71 649.0 0 71 301 136 649.0 0 136 Finished in 8.73 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 183,491 Reads with adapters: 69,924 (38.1%) Reads that were too short: 29 (0.0%) Reads written (passing filters): 69,895 (38.1%) Total basepairs processed: 46,649,376 bp Total written (filtered): 19,095,999 bp (40.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 69924 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 20 0.0 3 0 0 18 1 1 19 666 0.0 3 0 651 14 1 20 68189 0.0 4 66859 1278 50 2 21 150 0.0 4 29 120 1 22 15 0.0 4 1 5 8 0 1 23 298 0.0 4 251 7 0 34 6 24 1 0.0 4 0 0 0 0 1 25 1 0.0 4 1 27 1 0.0 4 0 0 0 1 28 36 0.0 4 0 0 0 1 35 32 1 0.0 4 1 35 1 0.0 4 1 37 1 0.0 4 0 0 0 0 1 51 1 0.0 4 1 62 2 0.0 4 0 0 0 1 1 65 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 8 0.0 4 0 0 0 0 8 127 500 0.0 4 0 0 0 0 500 128 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 1 152 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 212 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 223 1 0.0 4 0 0 0 0 1 227 2 0.0 4 0 0 0 0 2 228 6 0.0 4 0 0 0 0 6 229 1 0.0 4 0 0 0 0 1 230 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 255 2 0.0 4 0 0 0 0 2 271 1 0.0 4 0 0 0 0 1 280 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 284 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.39 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 69,895 Reads with adapters: 918 (1.3%) Reads written (passing filters): 69,895 (100.0%) Total basepairs processed: 19,095,999 bp Total written (filtered): 19,048,963 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 918 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 24.4% C: 8.0% G: 54.0% T: 11.9% none/other: 1.7% Overview of removed sequences length count expect max.err error counts 3 113 1092.1 0 113 4 14 273.0 0 14 5 37 68.3 0 37 6 2 17.1 0 2 8 1 1.1 0 1 9 17 0.3 0 0 17 10 32 0.1 1 2 30 11 362 0.0 1 5 357 12 82 0.0 1 2 80 13 4 0.0 1 0 4 15 2 0.0 1 0 2 20 49 0.0 1 42 7 21 19 0.0 1 16 3 24 21 0.0 1 16 5 25 2 0.0 1 2 26 1 0.0 1 1 37 1 0.0 1 0 1 47 1 0.0 1 0 1 79 1 0.0 1 1 95 2 0.0 1 2 108 1 0.0 1 1 125 1 0.0 1 0 1 151 1 0.0 1 0 1 152 7 0.0 1 0 7 153 3 0.0 1 0 3 186 5 0.0 1 5 187 2 0.0 1 0 2 210 19 0.0 1 4 15 211 2 0.0 1 0 2 225 2 0.0 1 2 230 1 0.0 1 0 1 268 3 0.0 1 3 269 1 0.0 1 1 271 6 0.0 1 6 273 75 0.0 1 71 4 274 24 0.0 1 21 3 275 2 0.0 1 2