This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCTCCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.95 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 151,846 (5.7%) Reads that were too short: 16,227 (0.6%) Reads written (passing filters): 135,619 (5.1%) Total basepairs processed: 800,111,879 bp Total written (filtered): 29,702,342 bp (3.7%) === Adapter 1 === Sequence: GCTCCG; Type: regular 5'; Length: 6; Trimmed: 151846 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2457 649.0 0 2457 7 30916 649.0 0 30916 8 1526 649.0 0 1526 9 27934 649.0 0 27934 10 72 649.0 0 72 11 25 649.0 0 25 12 27 649.0 0 27 13 31 649.0 0 31 14 18 649.0 0 18 15 34 649.0 0 34 16 17 649.0 0 17 17 18 649.0 0 18 18 30 649.0 0 30 19 22 649.0 0 22 20 26 649.0 0 26 21 15 649.0 0 15 22 18 649.0 0 18 23 38 649.0 0 38 24 21 649.0 0 21 25 21 649.0 0 21 26 21 649.0 0 21 27 33 649.0 0 33 28 15 649.0 0 15 29 43 649.0 0 43 30 45 649.0 0 45 31 24 649.0 0 24 32 27 649.0 0 27 33 32 649.0 0 32 34 32 649.0 0 32 35 26 649.0 0 26 36 23 649.0 0 23 37 37 649.0 0 37 38 36 649.0 0 36 39 35 649.0 0 35 40 130 649.0 0 130 41 28 649.0 0 28 42 47 649.0 0 47 43 16 649.0 0 16 44 25 649.0 0 25 45 27 649.0 0 27 46 36 649.0 0 36 47 51 649.0 0 51 48 34 649.0 0 34 49 39 649.0 0 39 50 57 649.0 0 57 51 33 649.0 0 33 52 28 649.0 0 28 53 24 649.0 0 24 54 24 649.0 0 24 55 97 649.0 0 97 56 73 649.0 0 73 57 79 649.0 0 79 58 38 649.0 0 38 59 40 649.0 0 40 60 55 649.0 0 55 61 29 649.0 0 29 62 24 649.0 0 24 63 28 649.0 0 28 64 27 649.0 0 27 65 52 649.0 0 52 66 98 649.0 0 98 67 36 649.0 0 36 68 60 649.0 0 60 69 51 649.0 0 51 70 40 649.0 0 40 71 60 649.0 0 60 72 33 649.0 0 33 73 46 649.0 0 46 74 80 649.0 0 80 75 72 649.0 0 72 76 33 649.0 0 33 77 22 649.0 0 22 78 20 649.0 0 20 79 31 649.0 0 31 80 20 649.0 0 20 81 35 649.0 0 35 82 56 649.0 0 56 83 73 649.0 0 73 84 54 649.0 0 54 85 38 649.0 0 38 86 32 649.0 0 32 87 29 649.0 0 29 88 36 649.0 0 36 89 45 649.0 0 45 90 66 649.0 0 66 91 130 649.0 0 130 92 52 649.0 0 52 93 54 649.0 0 54 94 31 649.0 0 31 95 53 649.0 0 53 96 26 649.0 0 26 97 43 649.0 0 43 98 26 649.0 0 26 99 26 649.0 0 26 100 30 649.0 0 30 101 36 649.0 0 36 102 63 649.0 0 63 103 286 649.0 0 286 104 77 649.0 0 77 105 626 649.0 0 626 106 328 649.0 0 328 107 360 649.0 0 360 108 30 649.0 0 30 109 27 649.0 0 27 110 36 649.0 0 36 111 56 649.0 0 56 112 226 649.0 0 226 113 93 649.0 0 93 114 257 649.0 0 257 115 68 649.0 0 68 116 86 649.0 0 86 117 41 649.0 0 41 118 42 649.0 0 42 119 40 649.0 0 40 120 36 649.0 0 36 121 50 649.0 0 50 122 38 649.0 0 38 123 47 649.0 0 47 124 39 649.0 0 39 125 49 649.0 0 49 126 66 649.0 0 66 127 97 649.0 0 97 128 120 649.0 0 120 129 210 649.0 0 210 130 118 649.0 0 118 131 102 649.0 0 102 132 129 649.0 0 129 133 166 649.0 0 166 134 376 649.0 0 376 135 117 649.0 0 117 136 158 649.0 0 158 137 272 649.0 0 272 138 113 649.0 0 113 139 134 649.0 0 134 140 212 649.0 0 212 141 199 649.0 0 199 142 309 649.0 0 309 143 329 649.0 0 329 144 476 649.0 0 476 145 592 649.0 0 592 146 1008 649.0 0 1008 147 20261 649.0 0 20261 148 9237 649.0 0 9237 149 21296 649.0 0 21296 150 408 649.0 0 408 151 98 649.0 0 98 152 409 649.0 0 409 153 110 649.0 0 110 154 416 649.0 0 416 155 214 649.0 0 214 156 166 649.0 0 166 157 49 649.0 0 49 158 32 649.0 0 32 159 56 649.0 0 56 160 47 649.0 0 47 161 38 649.0 0 38 162 38 649.0 0 38 163 48 649.0 0 48 164 44 649.0 0 44 165 49 649.0 0 49 166 36 649.0 0 36 167 51 649.0 0 51 168 148 649.0 0 148 169 130 649.0 0 130 170 160 649.0 0 160 171 174 649.0 0 174 172 271 649.0 0 271 173 132 649.0 0 132 174 158 649.0 0 158 175 81 649.0 0 81 176 27 649.0 0 27 177 115 649.0 0 115 178 64 649.0 0 64 179 71 649.0 0 71 180 38 649.0 0 38 181 33 649.0 0 33 182 67 649.0 0 67 183 70 649.0 0 70 184 74 649.0 0 74 185 59 649.0 0 59 186 133 649.0 0 133 187 128 649.0 0 128 188 1003 649.0 0 1003 189 695 649.0 0 695 190 643 649.0 0 643 191 152 649.0 0 152 192 56 649.0 0 56 193 35 649.0 0 35 194 37 649.0 0 37 195 49 649.0 0 49 196 112 649.0 0 112 197 113 649.0 0 113 198 115 649.0 0 115 199 261 649.0 0 261 200 400 649.0 0 400 201 1899 649.0 0 1899 202 1770 649.0 0 1770 203 1365 649.0 0 1365 204 486 649.0 0 486 205 104 649.0 0 104 206 64 649.0 0 64 207 42 649.0 0 42 208 114 649.0 0 114 209 44 649.0 0 44 210 56 649.0 0 56 211 29 649.0 0 29 212 22 649.0 0 22 213 21 649.0 0 21 214 40 649.0 0 40 215 50 649.0 0 50 216 43 649.0 0 43 217 37 649.0 0 37 218 24 649.0 0 24 219 46 649.0 0 46 220 41 649.0 0 41 221 68 649.0 0 68 222 60 649.0 0 60 223 81 649.0 0 81 224 84 649.0 0 84 225 98 649.0 0 98 226 157 649.0 0 157 227 178 649.0 0 178 228 171 649.0 0 171 229 236 649.0 0 236 230 115 649.0 0 115 231 71 649.0 0 71 232 47 649.0 0 47 233 45 649.0 0 45 234 42 649.0 0 42 235 53 649.0 0 53 236 73 649.0 0 73 237 48 649.0 0 48 238 62 649.0 0 62 239 44 649.0 0 44 240 34 649.0 0 34 241 29 649.0 0 29 242 35 649.0 0 35 243 78 649.0 0 78 244 74 649.0 0 74 245 70 649.0 0 70 246 44 649.0 0 44 247 88 649.0 0 88 248 95 649.0 0 95 249 105 649.0 0 105 250 120 649.0 0 120 251 113 649.0 0 113 252 98 649.0 0 98 253 71 649.0 0 71 254 90 649.0 0 90 255 24 649.0 0 24 256 57 649.0 0 57 257 80 649.0 0 80 258 180 649.0 0 180 259 150 649.0 0 150 260 142 649.0 0 142 261 90 649.0 0 90 262 84 649.0 0 84 263 60 649.0 0 60 264 78 649.0 0 78 265 62 649.0 0 62 266 69 649.0 0 69 267 60 649.0 0 60 268 74 649.0 0 74 269 556 649.0 0 556 270 275 649.0 0 275 271 227 649.0 0 227 272 89 649.0 0 89 273 94 649.0 0 94 274 112 649.0 0 112 275 199 649.0 0 199 276 160 649.0 0 160 277 125 649.0 0 125 278 77 649.0 0 77 279 94 649.0 0 94 280 135 649.0 0 135 281 96 649.0 0 96 282 350 649.0 0 350 283 208 649.0 0 208 284 168 649.0 0 168 285 71 649.0 0 71 286 147 649.0 0 147 287 94 649.0 0 94 288 167 649.0 0 167 289 167 649.0 0 167 290 102 649.0 0 102 291 474 649.0 0 474 292 487 649.0 0 487 293 565 649.0 0 565 294 847 649.0 0 847 295 621 649.0 0 621 296 486 649.0 0 486 297 185 649.0 0 185 298 59 649.0 0 59 299 94 649.0 0 94 300 111 649.0 0 111 301 100 649.0 0 100 Finished in 5.62 s (41 us/read; 1.45 M reads/minute). === Summary === Total reads processed: 135,619 Reads with adapters: 33,361 (24.6%) Reads that were too short: 231 (0.2%) Reads written (passing filters): 33,130 (24.4%) Total basepairs processed: 29,702,342 bp Total written (filtered): 9,111,394 bp (30.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 33361 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 8 0.0 3 0 0 5 3 16 256 0.0 3 0 206 27 23 17 32096 0.0 3 30358 1438 237 63 18 107 0.0 3 15 77 7 8 19 216 0.0 3 5 1 77 133 20 111 0.0 3 0 0 0 111 21 1 0.0 3 0 0 0 1 22 17 0.0 3 0 0 0 17 23 1 0.0 3 0 0 0 1 24 1 0.0 3 0 0 0 1 26 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 31 4 0.0 3 0 0 0 4 32 2 0.0 3 0 0 0 2 35 2 0.0 3 1 0 0 1 41 2 0.0 3 0 0 0 2 42 3 0.0 3 0 0 0 3 43 13 0.0 3 0 0 0 13 44 1 0.0 3 0 0 0 1 45 171 0.0 3 0 0 0 171 46 9 0.0 3 0 0 0 9 47 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 52 3 0.0 3 1 0 0 2 54 1 0.0 3 1 57 4 0.0 3 0 0 0 4 59 3 0.0 3 0 0 1 2 60 2 0.0 3 0 0 0 2 61 2 0.0 3 0 0 0 2 62 1 0.0 3 0 0 0 1 64 4 0.0 3 0 0 0 4 67 2 0.0 3 0 1 0 1 68 1 0.0 3 0 0 0 1 70 22 0.0 3 0 0 0 22 71 1 0.0 3 0 0 0 1 72 2 0.0 3 0 0 0 2 73 1 0.0 3 0 0 0 1 74 1 0.0 3 1 76 1 0.0 3 0 0 0 1 79 94 0.0 3 0 0 2 92 80 9 0.0 3 0 0 0 9 82 3 0.0 3 0 0 0 3 83 1 0.0 3 0 0 0 1 86 10 0.0 3 0 0 0 10 87 16 0.0 3 0 0 1 15 88 1 0.0 3 0 0 0 1 89 2 0.0 3 0 0 0 2 90 2 0.0 3 0 0 1 1 91 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 98 2 0.0 3 0 0 0 2 100 2 0.0 3 0 0 0 2 101 3 0.0 3 0 0 0 3 103 1 0.0 3 0 0 0 1 105 2 0.0 3 1 0 0 1 106 1 0.0 3 0 0 1 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 112 3 0.0 3 0 0 0 3 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 119 3 0.0 3 0 0 0 3 121 2 0.0 3 0 0 0 2 122 5 0.0 3 0 0 0 5 127 1 0.0 3 0 0 0 1 131 3 0.0 3 0 0 0 3 132 4 0.0 3 0 0 0 4 133 7 0.0 3 0 0 0 7 134 1 0.0 3 0 0 0 1 135 3 0.0 3 0 0 1 2 136 2 0.0 3 0 0 0 2 137 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 139 2 0.0 3 0 0 0 2 140 2 0.0 3 0 0 0 2 143 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 152 1 0.0 3 1 156 11 0.0 3 0 0 0 11 159 3 0.0 3 0 0 0 3 165 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 172 2 0.0 3 0 0 0 2 173 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 188 2 0.0 3 0 0 0 2 189 2 0.0 3 0 0 0 2 190 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 194 4 0.0 3 0 0 0 4 195 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 1 205 2 0.0 3 0 0 0 2 216 6 0.0 3 0 0 0 6 220 1 0.0 3 0 0 0 1 221 3 0.0 3 0 0 0 3 225 2 0.0 3 0 0 0 2 229 1 0.0 3 0 0 0 1 249 1 0.0 3 0 0 0 1 270 2 0.0 3 0 0 0 2 279 3 0.0 3 0 0 0 3 280 3 0.0 3 0 0 0 3 281 7 0.0 3 0 0 0 7 282 2 0.0 3 0 0 0 2 283 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.16 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 33,130 Reads with adapters: 509 (1.5%) Reads written (passing filters): 33,130 (100.0%) Total basepairs processed: 9,111,394 bp Total written (filtered): 9,109,833 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 509 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 14.7% C: 45.4% G: 29.7% T: 10.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 497 517.7 0 497 4 4 129.4 0 4 5 5 32.4 0 5 6 1 8.1 0 1 11 1 0.0 1 0 1 12 1 0.0 1 0 1