This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCTCCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 69.75 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 142,235 (5.4%) Reads that were too short: 8,909 (0.3%) Reads written (passing filters): 133,326 (5.0%) Total basepairs processed: 800,111,879 bp Total written (filtered): 29,562,383 bp (3.7%) === Adapter 1 === Sequence: GCTCCG; Type: regular 5'; Length: 6; Trimmed: 142235 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2228 649.0 0 2228 7 29224 649.0 0 29224 8 1256 649.0 0 1256 9 32554 649.0 0 32554 10 54 649.0 0 54 11 19 649.0 0 19 12 15 649.0 0 15 13 9 649.0 0 9 14 10 649.0 0 10 15 24 649.0 0 24 16 12 649.0 0 12 17 13 649.0 0 13 18 15 649.0 0 15 19 13 649.0 0 13 20 9 649.0 0 9 21 17 649.0 0 17 22 14 649.0 0 14 23 10 649.0 0 10 24 13 649.0 0 13 25 9 649.0 0 9 26 15 649.0 0 15 27 16 649.0 0 16 28 16 649.0 0 16 29 12 649.0 0 12 30 19 649.0 0 19 31 15 649.0 0 15 32 18 649.0 0 18 33 13 649.0 0 13 34 20 649.0 0 20 35 17 649.0 0 17 36 16 649.0 0 16 37 23 649.0 0 23 38 20 649.0 0 20 39 13 649.0 0 13 40 35 649.0 0 35 41 20 649.0 0 20 42 18 649.0 0 18 43 15 649.0 0 15 44 10 649.0 0 10 45 8 649.0 0 8 46 22 649.0 0 22 47 35 649.0 0 35 48 24 649.0 0 24 49 20 649.0 0 20 50 51 649.0 0 51 51 24 649.0 0 24 52 20 649.0 0 20 53 13 649.0 0 13 54 13 649.0 0 13 55 40 649.0 0 40 56 49 649.0 0 49 57 31 649.0 0 31 58 23 649.0 0 23 59 23 649.0 0 23 60 16 649.0 0 16 61 13 649.0 0 13 62 12 649.0 0 12 63 14 649.0 0 14 64 12 649.0 0 12 65 33 649.0 0 33 66 60 649.0 0 60 67 14 649.0 0 14 68 15 649.0 0 15 69 25 649.0 0 25 70 15 649.0 0 15 71 43 649.0 0 43 72 24 649.0 0 24 73 18 649.0 0 18 74 56 649.0 0 56 75 42 649.0 0 42 76 21 649.0 0 21 77 20 649.0 0 20 78 18 649.0 0 18 79 28 649.0 0 28 80 23 649.0 0 23 81 37 649.0 0 37 82 46 649.0 0 46 83 24 649.0 0 24 84 19 649.0 0 19 85 26 649.0 0 26 86 16 649.0 0 16 87 31 649.0 0 31 88 23 649.0 0 23 89 29 649.0 0 29 90 34 649.0 0 34 91 48 649.0 0 48 92 18 649.0 0 18 93 32 649.0 0 32 94 27 649.0 0 27 95 23 649.0 0 23 96 25 649.0 0 25 97 21 649.0 0 21 98 18 649.0 0 18 99 12 649.0 0 12 100 15 649.0 0 15 101 32 649.0 0 32 102 38 649.0 0 38 103 265 649.0 0 265 104 38 649.0 0 38 105 249 649.0 0 249 106 164 649.0 0 164 107 179 649.0 0 179 108 23 649.0 0 23 109 27 649.0 0 27 110 35 649.0 0 35 111 29 649.0 0 29 112 68 649.0 0 68 113 59 649.0 0 59 114 130 649.0 0 130 115 41 649.0 0 41 116 58 649.0 0 58 117 43 649.0 0 43 118 23 649.0 0 23 119 27 649.0 0 27 120 33 649.0 0 33 121 36 649.0 0 36 122 29 649.0 0 29 123 64 649.0 0 64 124 35 649.0 0 35 125 29 649.0 0 29 126 36 649.0 0 36 127 69 649.0 0 69 128 102 649.0 0 102 129 154 649.0 0 154 130 106 649.0 0 106 131 81 649.0 0 81 132 88 649.0 0 88 133 180 649.0 0 180 134 374 649.0 0 374 135 118 649.0 0 118 136 112 649.0 0 112 137 253 649.0 0 253 138 96 649.0 0 96 139 108 649.0 0 108 140 252 649.0 0 252 141 202 649.0 0 202 142 257 649.0 0 257 143 305 649.0 0 305 144 414 649.0 0 414 145 470 649.0 0 470 146 768 649.0 0 768 147 20534 649.0 0 20534 148 9385 649.0 0 9385 149 22317 649.0 0 22317 150 371 649.0 0 371 151 94 649.0 0 94 152 342 649.0 0 342 153 48 649.0 0 48 154 297 649.0 0 297 155 164 649.0 0 164 156 124 649.0 0 124 157 19 649.0 0 19 158 23 649.0 0 23 159 28 649.0 0 28 160 21 649.0 0 21 161 28 649.0 0 28 162 28 649.0 0 28 163 32 649.0 0 32 164 19 649.0 0 19 165 26 649.0 0 26 166 32 649.0 0 32 167 36 649.0 0 36 168 99 649.0 0 99 169 75 649.0 0 75 170 123 649.0 0 123 171 192 649.0 0 192 172 167 649.0 0 167 173 61 649.0 0 61 174 113 649.0 0 113 175 43 649.0 0 43 176 14 649.0 0 14 177 35 649.0 0 35 178 40 649.0 0 40 179 35 649.0 0 35 180 21 649.0 0 21 181 18 649.0 0 18 182 34 649.0 0 34 183 39 649.0 0 39 184 46 649.0 0 46 185 35 649.0 0 35 186 64 649.0 0 64 187 67 649.0 0 67 188 762 649.0 0 762 189 481 649.0 0 481 190 523 649.0 0 523 191 110 649.0 0 110 192 22 649.0 0 22 193 23 649.0 0 23 194 18 649.0 0 18 195 26 649.0 0 26 196 39 649.0 0 39 197 77 649.0 0 77 198 71 649.0 0 71 199 135 649.0 0 135 200 264 649.0 0 264 201 1896 649.0 0 1896 202 1843 649.0 0 1843 203 1400 649.0 0 1400 204 398 649.0 0 398 205 42 649.0 0 42 206 20 649.0 0 20 207 12 649.0 0 12 208 34 649.0 0 34 209 16 649.0 0 16 210 33 649.0 0 33 211 15 649.0 0 15 212 27 649.0 0 27 213 18 649.0 0 18 214 20 649.0 0 20 215 27 649.0 0 27 216 21 649.0 0 21 217 18 649.0 0 18 218 13 649.0 0 13 219 23 649.0 0 23 220 16 649.0 0 16 221 33 649.0 0 33 222 45 649.0 0 45 223 19 649.0 0 19 224 29 649.0 0 29 225 40 649.0 0 40 226 88 649.0 0 88 227 163 649.0 0 163 228 137 649.0 0 137 229 129 649.0 0 129 230 69 649.0 0 69 231 24 649.0 0 24 232 31 649.0 0 31 233 18 649.0 0 18 234 30 649.0 0 30 235 27 649.0 0 27 236 49 649.0 0 49 237 31 649.0 0 31 238 55 649.0 0 55 239 36 649.0 0 36 240 22 649.0 0 22 241 31 649.0 0 31 242 24 649.0 0 24 243 26 649.0 0 26 244 27 649.0 0 27 245 36 649.0 0 36 246 19 649.0 0 19 247 32 649.0 0 32 248 33 649.0 0 33 249 40 649.0 0 40 250 45 649.0 0 45 251 45 649.0 0 45 252 44 649.0 0 44 253 17 649.0 0 17 254 40 649.0 0 40 255 22 649.0 0 22 256 34 649.0 0 34 257 54 649.0 0 54 258 103 649.0 0 103 259 116 649.0 0 116 260 50 649.0 0 50 261 35 649.0 0 35 262 25 649.0 0 25 263 21 649.0 0 21 264 26 649.0 0 26 265 21 649.0 0 21 266 20 649.0 0 20 267 35 649.0 0 35 268 30 649.0 0 30 269 226 649.0 0 226 270 104 649.0 0 104 271 78 649.0 0 78 272 30 649.0 0 30 273 45 649.0 0 45 274 64 649.0 0 64 275 76 649.0 0 76 276 60 649.0 0 60 277 69 649.0 0 69 278 40 649.0 0 40 279 31 649.0 0 31 280 46 649.0 0 46 281 31 649.0 0 31 282 152 649.0 0 152 283 84 649.0 0 84 284 67 649.0 0 67 285 45 649.0 0 45 286 64 649.0 0 64 287 40 649.0 0 40 288 86 649.0 0 86 289 67 649.0 0 67 290 33 649.0 0 33 291 180 649.0 0 180 292 150 649.0 0 150 293 218 649.0 0 218 294 270 649.0 0 270 295 131 649.0 0 131 296 100 649.0 0 100 297 66 649.0 0 66 298 18 649.0 0 18 299 39 649.0 0 39 300 39 649.0 0 39 301 38 649.0 0 38 Finished in 5.90 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 133,326 Reads with adapters: 34,880 (26.2%) Reads that were too short: 31 (0.0%) Reads written (passing filters): 34,849 (26.1%) Total basepairs processed: 29,562,383 bp Total written (filtered): 9,423,811 bp (31.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 34880 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 15 0.0 3 0 0 14 1 19 448 0.0 3 0 435 11 1 1 20 33731 0.0 4 33021 685 21 2 2 21 59 0.0 4 15 41 2 0 1 22 19 0.0 4 12 0 5 0 2 23 21 0.0 4 1 0 0 20 24 2 0.0 4 1 0 0 0 1 25 1 0.0 4 1 27 2 0.0 4 2 35 2 0.0 4 0 0 0 0 2 40 1 0.0 4 1 55 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 0 1 78 2 0.0 4 0 0 0 0 2 85 1 0.0 4 0 1 89 5 0.0 4 0 0 0 0 5 95 1 0.0 4 0 0 0 0 1 110 1 0.0 4 1 125 2 0.0 4 0 0 0 0 2 126 6 0.0 4 0 0 0 0 6 127 533 0.0 4 0 0 0 0 533 128 3 0.0 4 0 0 0 0 3 130 1 0.0 4 0 0 0 0 1 134 1 0.0 4 1 142 1 0.0 4 1 148 1 0.0 4 0 1 163 1 0.0 4 0 0 0 0 1 195 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 0 3 229 1 0.0 4 0 0 0 0 1 255 1 0.0 4 0 1 257 2 0.0 4 2 258 1 0.0 4 0 1 264 3 0.0 4 0 0 0 0 3 275 1 0.0 4 0 0 0 0 1 276 1 0.0 4 0 0 0 0 1 281 2 0.0 4 0 0 0 0 2 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.24 s (36 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 34,849 Reads with adapters: 4,303 (12.3%) Reads written (passing filters): 34,849 (100.0%) Total basepairs processed: 9,423,811 bp Total written (filtered): 9,340,208 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 4303 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.6% C: 3.7% G: 92.7% T: 1.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 39 544.5 0 39 4 14 136.1 0 14 5 1 34.0 0 1 6 1 8.5 0 1 9 1100 0.1 0 39 1061 10 2316 0.0 1 80 2236 11 628 0.0 1 4 624 12 3 0.0 1 0 3 13 2 0.0 1 0 2 18 6 0.0 1 5 1 19 1 0.0 1 0 1 21 1 0.0 1 0 1 22 4 0.0 1 3 1 25 1 0.0 1 0 1 115 2 0.0 1 1 1 146 1 0.0 1 1 150 1 0.0 1 0 1 169 1 0.0 1 1 207 7 0.0 1 6 1 208 1 0.0 1 0 1 226 1 0.0 1 1 232 8 0.0 1 7 1 233 1 0.0 1 1 234 2 0.0 1 2 235 144 0.0 1 129 15 236 10 0.0 1 10 237 2 0.0 1 1 1 238 1 0.0 1 1 268 1 0.0 1 1 271 3 0.0 1 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.