This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACACAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.57 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 293,793 (11.1%) Reads that were too short: 67,862 (2.6%) Reads written (passing filters): 225,931 (8.5%) Total basepairs processed: 800,111,879 bp Total written (filtered): 58,984,607 bp (7.4%) === Adapter 1 === Sequence: ACACAA; Type: regular 5'; Length: 6; Trimmed: 293793 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1376 649.0 0 1376 7 87477 649.0 0 87477 8 1052 649.0 0 1052 9 78822 649.0 0 78822 10 73 649.0 0 73 11 23 649.0 0 23 12 14 649.0 0 14 13 11 649.0 0 11 14 10 649.0 0 10 15 29 649.0 0 29 16 18 649.0 0 18 17 17 649.0 0 17 18 13 649.0 0 13 19 16 649.0 0 16 20 16 649.0 0 16 21 19 649.0 0 19 22 23 649.0 0 23 23 18 649.0 0 18 24 11 649.0 0 11 25 12 649.0 0 12 26 11 649.0 0 11 27 31 649.0 0 31 28 30 649.0 0 30 29 20 649.0 0 20 30 22 649.0 0 22 31 21 649.0 0 21 32 22 649.0 0 22 33 25 649.0 0 25 34 41 649.0 0 41 35 13 649.0 0 13 36 25 649.0 0 25 37 22 649.0 0 22 38 28 649.0 0 28 39 32 649.0 0 32 40 37 649.0 0 37 41 290 649.0 0 290 42 181 649.0 0 181 43 222 649.0 0 222 44 33 649.0 0 33 45 36 649.0 0 36 46 41 649.0 0 41 47 32 649.0 0 32 48 35 649.0 0 35 49 22 649.0 0 22 50 53 649.0 0 53 51 208 649.0 0 208 52 45 649.0 0 45 53 17 649.0 0 17 54 38 649.0 0 38 55 33 649.0 0 33 56 651 649.0 0 651 57 30 649.0 0 30 58 44 649.0 0 44 59 29 649.0 0 29 60 35 649.0 0 35 61 20 649.0 0 20 62 38 649.0 0 38 63 36 649.0 0 36 64 25 649.0 0 25 65 18 649.0 0 18 66 37 649.0 0 37 67 84 649.0 0 84 68 181 649.0 0 181 69 24 649.0 0 24 70 59 649.0 0 59 71 70 649.0 0 70 72 84 649.0 0 84 73 85 649.0 0 85 74 144 649.0 0 144 75 82 649.0 0 82 76 95 649.0 0 95 77 163 649.0 0 163 78 188 649.0 0 188 79 336 649.0 0 336 80 420 649.0 0 420 81 368 649.0 0 368 82 515 649.0 0 515 83 536 649.0 0 536 84 537 649.0 0 537 85 412 649.0 0 412 86 270 649.0 0 270 87 221 649.0 0 221 88 162 649.0 0 162 89 177 649.0 0 177 90 163 649.0 0 163 91 299 649.0 0 299 92 237 649.0 0 237 93 267 649.0 0 267 94 212 649.0 0 212 95 207 649.0 0 207 96 178 649.0 0 178 97 309 649.0 0 309 98 174 649.0 0 174 99 144 649.0 0 144 100 133 649.0 0 133 101 165 649.0 0 165 102 244 649.0 0 244 103 270 649.0 0 270 104 368 649.0 0 368 105 527 649.0 0 527 106 542 649.0 0 542 107 582 649.0 0 582 108 421 649.0 0 421 109 417 649.0 0 417 110 377 649.0 0 377 111 479 649.0 0 479 112 448 649.0 0 448 113 572 649.0 0 572 114 513 649.0 0 513 115 531 649.0 0 531 116 460 649.0 0 460 117 380 649.0 0 380 118 576 649.0 0 576 119 517 649.0 0 517 120 604 649.0 0 604 121 429 649.0 0 429 122 465 649.0 0 465 123 411 649.0 0 411 124 526 649.0 0 526 125 642 649.0 0 642 126 594 649.0 0 594 127 393 649.0 0 393 128 423 649.0 0 423 129 331 649.0 0 331 130 421 649.0 0 421 131 387 649.0 0 387 132 494 649.0 0 494 133 1964 649.0 0 1964 134 1995 649.0 0 1995 135 1864 649.0 0 1864 136 458 649.0 0 458 137 357 649.0 0 357 138 373 649.0 0 373 139 345 649.0 0 345 140 475 649.0 0 475 141 325 649.0 0 325 142 352 649.0 0 352 143 395 649.0 0 395 144 374 649.0 0 374 145 359 649.0 0 359 146 471 649.0 0 471 147 369 649.0 0 369 148 354 649.0 0 354 149 359 649.0 0 359 150 348 649.0 0 348 151 328 649.0 0 328 152 353 649.0 0 353 153 316 649.0 0 316 154 423 649.0 0 423 155 347 649.0 0 347 156 364 649.0 0 364 157 368 649.0 0 368 158 391 649.0 0 391 159 491 649.0 0 491 160 487 649.0 0 487 161 503 649.0 0 503 162 916 649.0 0 916 163 865 649.0 0 865 164 880 649.0 0 880 165 427 649.0 0 427 166 355 649.0 0 355 167 321 649.0 0 321 168 331 649.0 0 331 169 321 649.0 0 321 170 351 649.0 0 351 171 386 649.0 0 386 172 465 649.0 0 465 173 393 649.0 0 393 174 336 649.0 0 336 175 274 649.0 0 274 176 277 649.0 0 277 177 283 649.0 0 283 178 345 649.0 0 345 179 430 649.0 0 430 180 389 649.0 0 389 181 299 649.0 0 299 182 398 649.0 0 398 183 566 649.0 0 566 184 411 649.0 0 411 185 457 649.0 0 457 186 338 649.0 0 338 187 405 649.0 0 405 188 354 649.0 0 354 189 434 649.0 0 434 190 434 649.0 0 434 191 442 649.0 0 442 192 475 649.0 0 475 193 376 649.0 0 376 194 324 649.0 0 324 195 227 649.0 0 227 196 313 649.0 0 313 197 282 649.0 0 282 198 329 649.0 0 329 199 348 649.0 0 348 200 364 649.0 0 364 201 378 649.0 0 378 202 368 649.0 0 368 203 372 649.0 0 372 204 295 649.0 0 295 205 360 649.0 0 360 206 333 649.0 0 333 207 433 649.0 0 433 208 448 649.0 0 448 209 481 649.0 0 481 210 482 649.0 0 482 211 975 649.0 0 975 212 1806 649.0 0 1806 213 5727 649.0 0 5727 214 6360 649.0 0 6360 215 10945 649.0 0 10945 216 658 649.0 0 658 217 1246 649.0 0 1246 218 513 649.0 0 513 219 533 649.0 0 533 220 373 649.0 0 373 221 371 649.0 0 371 222 399 649.0 0 399 223 393 649.0 0 393 224 408 649.0 0 408 225 567 649.0 0 567 226 742 649.0 0 742 227 811 649.0 0 811 228 484 649.0 0 484 229 635 649.0 0 635 230 488 649.0 0 488 231 537 649.0 0 537 232 338 649.0 0 338 233 305 649.0 0 305 234 316 649.0 0 316 235 326 649.0 0 326 236 313 649.0 0 313 237 353 649.0 0 353 238 338 649.0 0 338 239 291 649.0 0 291 240 272 649.0 0 272 241 349 649.0 0 349 242 365 649.0 0 365 243 314 649.0 0 314 244 277 649.0 0 277 245 306 649.0 0 306 246 346 649.0 0 346 247 386 649.0 0 386 248 400 649.0 0 400 249 476 649.0 0 476 250 683 649.0 0 683 251 798 649.0 0 798 252 774 649.0 0 774 253 512 649.0 0 512 254 610 649.0 0 610 255 437 649.0 0 437 256 390 649.0 0 390 257 387 649.0 0 387 258 370 649.0 0 370 259 385 649.0 0 385 260 339 649.0 0 339 261 300 649.0 0 300 262 343 649.0 0 343 263 327 649.0 0 327 264 399 649.0 0 399 265 467 649.0 0 467 266 421 649.0 0 421 267 378 649.0 0 378 268 406 649.0 0 406 269 407 649.0 0 407 270 442 649.0 0 442 271 441 649.0 0 441 272 350 649.0 0 350 273 377 649.0 0 377 274 443 649.0 0 443 275 428 649.0 0 428 276 466 649.0 0 466 277 478 649.0 0 478 278 540 649.0 0 540 279 480 649.0 0 480 280 424 649.0 0 424 281 449 649.0 0 449 282 525 649.0 0 525 283 462 649.0 0 462 284 518 649.0 0 518 285 440 649.0 0 440 286 446 649.0 0 446 287 441 649.0 0 441 288 388 649.0 0 388 289 521 649.0 0 521 290 490 649.0 0 490 291 483 649.0 0 483 292 395 649.0 0 395 293 341 649.0 0 341 294 385 649.0 0 385 295 380 649.0 0 380 296 391 649.0 0 391 297 386 649.0 0 386 298 367 649.0 0 367 299 349 649.0 0 349 300 390 649.0 0 390 301 699 649.0 0 699 Finished in 10.92 s (48 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 225,931 Reads with adapters: 89,116 (39.4%) Reads that were too short: 74 (0.0%) Reads written (passing filters): 89,042 (39.4%) Total basepairs processed: 58,984,607 bp Total written (filtered): 24,259,407 bp (41.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 89116 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 4 0.0 3 0 0 0 3 1 18 37 0.0 3 0 0 34 1 2 19 1454 0.0 3 0 1412 38 1 3 20 86047 0.0 4 84440 1553 51 2 1 21 160 0.0 4 37 121 2 22 16 0.0 4 5 1 9 0 1 23 45 0.0 4 0 0 0 43 2 24 1 0.0 4 0 0 0 0 1 25 1 0.0 4 0 0 0 0 1 26 1 0.0 4 0 1 29 2 0.0 4 2 30 1 0.0 4 0 0 0 0 1 35 1 0.0 4 0 0 0 0 1 36 1 0.0 4 0 0 0 0 1 38 2 0.0 4 0 0 0 0 2 47 2 0.0 4 0 0 0 0 2 51 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 1 71 3 0.0 4 0 0 0 0 3 72 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 83 3 0.0 4 0 0 0 0 3 85 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 92 2 0.0 4 0 0 0 0 2 93 1 0.0 4 0 1 94 2 0.0 4 1 0 0 0 1 96 2 0.0 4 0 0 0 0 2 98 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 114 2 0.0 4 0 0 0 1 1 115 1 0.0 4 0 0 0 0 1 119 1 0.0 4 0 0 0 0 1 124 2 0.0 4 1 0 1 125 5 0.0 4 0 0 0 0 5 126 12 0.0 4 0 0 0 0 12 127 1234 0.0 4 0 0 0 0 1234 130 2 0.0 4 0 0 0 0 2 131 1 0.0 4 0 0 0 0 1 133 2 0.0 4 0 0 0 0 2 135 1 0.0 4 0 0 0 0 1 142 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 1 152 1 0.0 4 0 0 0 0 1 159 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 1 170 2 0.0 4 1 0 0 0 1 172 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 184 1 0.0 4 0 0 0 0 1 186 1 0.0 4 0 0 0 0 1 188 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 0 0 0 1 194 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 221 3 0.0 4 0 0 0 0 3 228 4 0.0 4 0 0 0 0 4 229 3 0.0 4 0 0 0 0 3 231 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 235 2 0.0 4 1 0 0 0 1 243 1 0.0 4 0 1 252 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 289 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.04 s (34 us/read; 1.76 M reads/minute). === Summary === Total reads processed: 89,042 Reads with adapters: 2,202 (2.5%) Reads written (passing filters): 89,042 (100.0%) Total basepairs processed: 24,259,407 bp Total written (filtered): 24,222,047 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2202 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 59.7% C: 5.0% G: 33.4% T: 2.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 187 1391.3 0 187 4 19 347.8 0 19 5 4 87.0 0 4 6 4 21.7 0 4 7 1 5.4 0 1 8 1 1.4 0 1 9 2 0.3 0 1 1 10 25 0.1 1 1 24 11 635 0.0 1 7 628 12 111 0.0 1 5 106 13 7 0.0 1 0 7 15 2 0.0 1 0 2 16 1 0.0 1 0 1 19 6 0.0 1 4 2 20 1069 0.0 1 854 215 21 54 0.0 1 45 9 22 3 0.0 1 2 1 23 3 0.0 1 2 1 24 28 0.0 1 23 5 25 10 0.0 1 5 5 35 1 0.0 1 1 39 1 0.0 1 1 64 1 0.0 1 1 72 1 0.0 1 1 80 1 0.0 1 1 92 1 0.0 1 1 94 1 0.0 1 1 95 2 0.0 1 2 107 1 0.0 1 0 1 109 1 0.0 1 1 125 1 0.0 1 1 149 1 0.0 1 1 150 1 0.0 1 1 152 7 0.0 1 0 7 158 1 0.0 1 1 210 7 0.0 1 3 4 259 1 0.0 1 1