This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCGGTT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 68.98 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 354,056 (13.3%) Reads that were too short: 326,781 (12.3%) Reads written (passing filters): 27,275 (1.0%) Total basepairs processed: 800,111,879 bp Total written (filtered): 5,326,427 bp (0.7%) === Adapter 1 === Sequence: GCGGTT; Type: regular 5'; Length: 6; Trimmed: 354056 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 392 649.0 0 392 7 3358 649.0 0 3358 8 2670 649.0 0 2670 9 2180 649.0 0 2180 10 40 649.0 0 40 11 32 649.0 0 32 12 24 649.0 0 24 13 37 649.0 0 37 14 38 649.0 0 38 15 24 649.0 0 24 16 29 649.0 0 29 17 45 649.0 0 45 18 51 649.0 0 51 19 21 649.0 0 21 20 80 649.0 0 80 21 74 649.0 0 74 22 84 649.0 0 84 23 26 649.0 0 26 24 31 649.0 0 31 25 27 649.0 0 27 26 45 649.0 0 45 27 20 649.0 0 20 28 21 649.0 0 21 29 37 649.0 0 37 30 23 649.0 0 23 31 29 649.0 0 29 32 19 649.0 0 19 33 71 649.0 0 71 34 47 649.0 0 47 35 38 649.0 0 38 36 32 649.0 0 32 37 26 649.0 0 26 38 34 649.0 0 34 39 32 649.0 0 32 40 25 649.0 0 25 41 28 649.0 0 28 42 34 649.0 0 34 43 28 649.0 0 28 44 27 649.0 0 27 45 76 649.0 0 76 46 25 649.0 0 25 47 39 649.0 0 39 48 36 649.0 0 36 49 28 649.0 0 28 50 26 649.0 0 26 51 34 649.0 0 34 52 26 649.0 0 26 53 32 649.0 0 32 54 17 649.0 0 17 55 23 649.0 0 23 56 26 649.0 0 26 57 29 649.0 0 29 58 31 649.0 0 31 59 15 649.0 0 15 60 29 649.0 0 29 61 24 649.0 0 24 62 23 649.0 0 23 63 51 649.0 0 51 64 59 649.0 0 59 65 42 649.0 0 42 66 51 649.0 0 51 67 60 649.0 0 60 68 38 649.0 0 38 69 25 649.0 0 25 70 22 649.0 0 22 71 33 649.0 0 33 72 29 649.0 0 29 73 42 649.0 0 42 74 37 649.0 0 37 75 37 649.0 0 37 76 33 649.0 0 33 77 48 649.0 0 48 78 38 649.0 0 38 79 44 649.0 0 44 80 31 649.0 0 31 81 47 649.0 0 47 82 37 649.0 0 37 83 30 649.0 0 30 84 39 649.0 0 39 85 43 649.0 0 43 86 59 649.0 0 59 87 120 649.0 0 120 88 73 649.0 0 73 89 48 649.0 0 48 90 61 649.0 0 61 91 73 649.0 0 73 92 27 649.0 0 27 93 80 649.0 0 80 94 105 649.0 0 105 95 107 649.0 0 107 96 50 649.0 0 50 97 54 649.0 0 54 98 50 649.0 0 50 99 65 649.0 0 65 100 23 649.0 0 23 101 35 649.0 0 35 102 36 649.0 0 36 103 29 649.0 0 29 104 26 649.0 0 26 105 30 649.0 0 30 106 39 649.0 0 39 107 33 649.0 0 33 108 34 649.0 0 34 109 26 649.0 0 26 110 24 649.0 0 24 111 34 649.0 0 34 112 51 649.0 0 51 113 38 649.0 0 38 114 34 649.0 0 34 115 18 649.0 0 18 116 36 649.0 0 36 117 29 649.0 0 29 118 44 649.0 0 44 119 36 649.0 0 36 120 42 649.0 0 42 121 38 649.0 0 38 122 41 649.0 0 41 123 35 649.0 0 35 124 89 649.0 0 89 125 212 649.0 0 212 126 269 649.0 0 269 127 210 649.0 0 210 128 41 649.0 0 41 129 46 649.0 0 46 130 99 649.0 0 99 131 306 649.0 0 306 132 297 649.0 0 297 133 182 649.0 0 182 134 126 649.0 0 126 135 48 649.0 0 48 136 55 649.0 0 55 137 41 649.0 0 41 138 40 649.0 0 40 139 36 649.0 0 36 140 35 649.0 0 35 141 139 649.0 0 139 142 47 649.0 0 47 143 74 649.0 0 74 144 49 649.0 0 49 145 96 649.0 0 96 146 65 649.0 0 65 147 49 649.0 0 49 148 51 649.0 0 51 149 44 649.0 0 44 150 49 649.0 0 49 151 35 649.0 0 35 152 37 649.0 0 37 153 56 649.0 0 56 154 80 649.0 0 80 155 68 649.0 0 68 156 54 649.0 0 54 157 32 649.0 0 32 158 23 649.0 0 23 159 23 649.0 0 23 160 32 649.0 0 32 161 24 649.0 0 24 162 27 649.0 0 27 163 28 649.0 0 28 164 31 649.0 0 31 165 58 649.0 0 58 166 47 649.0 0 47 167 75 649.0 0 75 168 55 649.0 0 55 169 48 649.0 0 48 170 41 649.0 0 41 171 43 649.0 0 43 172 88 649.0 0 88 173 171 649.0 0 171 174 666 649.0 0 666 175 264 649.0 0 264 176 311 649.0 0 311 177 67 649.0 0 67 178 53 649.0 0 53 179 215 649.0 0 215 180 288 649.0 0 288 181 326 649.0 0 326 182 124 649.0 0 124 183 99 649.0 0 99 184 53 649.0 0 53 185 216 649.0 0 216 186 192 649.0 0 192 187 195 649.0 0 195 188 71 649.0 0 71 189 80 649.0 0 80 190 111 649.0 0 111 191 146 649.0 0 146 192 534 649.0 0 534 193 1731 649.0 0 1731 194 1039 649.0 0 1039 195 914 649.0 0 914 196 234 649.0 0 234 197 78 649.0 0 78 198 108 649.0 0 108 199 479 649.0 0 479 200 900 649.0 0 900 201 693 649.0 0 693 202 387 649.0 0 387 203 79 649.0 0 79 204 49 649.0 0 49 205 77 649.0 0 77 206 143 649.0 0 143 207 157 649.0 0 157 208 106 649.0 0 106 209 62 649.0 0 62 210 96 649.0 0 96 211 72 649.0 0 72 212 100 649.0 0 100 213 134 649.0 0 134 214 99 649.0 0 99 215 137 649.0 0 137 216 105 649.0 0 105 217 115 649.0 0 115 218 145 649.0 0 145 219 358 649.0 0 358 220 262 649.0 0 262 221 329 649.0 0 329 222 397 649.0 0 397 223 336 649.0 0 336 224 479 649.0 0 479 225 316 649.0 0 316 226 316 649.0 0 316 227 561 649.0 0 561 228 417 649.0 0 417 229 599 649.0 0 599 230 811 649.0 0 811 231 1551 649.0 0 1551 232 16523 649.0 0 16523 233 7417 649.0 0 7417 234 15586 649.0 0 15586 235 1165 649.0 0 1165 236 1519 649.0 0 1519 237 23015 649.0 0 23015 238 14645 649.0 0 14645 239 23088 649.0 0 23088 240 1571 649.0 0 1571 241 4258 649.0 0 4258 242 741 649.0 0 741 243 1911 649.0 0 1911 244 1133 649.0 0 1133 245 1544 649.0 0 1544 246 460 649.0 0 460 247 764 649.0 0 764 248 669 649.0 0 669 249 1118 649.0 0 1118 250 1544 649.0 0 1544 251 2121 649.0 0 2121 252 10413 649.0 0 10413 253 3451 649.0 0 3451 254 4498 649.0 0 4498 255 1742 649.0 0 1742 256 2920 649.0 0 2920 257 49577 649.0 0 49577 258 35900 649.0 0 35900 259 65431 649.0 0 65431 260 18523 649.0 0 18523 261 170 649.0 0 170 262 108 649.0 0 108 263 60 649.0 0 60 264 84 649.0 0 84 265 53 649.0 0 53 266 95 649.0 0 95 267 64 649.0 0 64 268 167 649.0 0 167 269 68 649.0 0 68 270 135 649.0 0 135 271 68 649.0 0 68 272 46 649.0 0 46 273 63 649.0 0 63 274 45 649.0 0 45 275 58 649.0 0 58 276 77 649.0 0 77 277 37 649.0 0 37 278 39 649.0 0 39 279 60 649.0 0 60 280 71 649.0 0 71 281 108 649.0 0 108 282 114 649.0 0 114 283 72 649.0 0 72 284 61 649.0 0 61 285 84 649.0 0 84 286 85 649.0 0 85 287 129 649.0 0 129 288 124 649.0 0 124 289 161 649.0 0 161 290 252 649.0 0 252 291 454 649.0 0 454 292 383 649.0 0 383 293 270 649.0 0 270 294 186 649.0 0 186 295 155 649.0 0 155 296 106 649.0 0 106 297 46 649.0 0 46 298 49 649.0 0 49 299 76 649.0 0 76 300 88 649.0 0 88 301 168 649.0 0 168 Finished in 1.10 s (40 us/read; 1.49 M reads/minute). === Summary === Total reads processed: 27,275 Reads with adapters: 6,251 (22.9%) Reads that were too short: 64 (0.2%) Reads written (passing filters): 6,187 (22.7%) Total basepairs processed: 5,326,427 bp Total written (filtered): 1,686,113 bp (31.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 6251 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 26 0.0 3 0 22 3 1 17 2512 0.0 3 2369 124 15 4 18 86 0.0 3 0 40 3 43 19 3446 0.0 3 3229 184 27 6 20 20 0.0 3 3 7 2 8 21 6 0.0 3 0 0 5 1 22 12 0.0 3 0 0 0 12 23 1 0.0 3 0 0 0 1 24 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 33 1 0.0 3 1 34 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 37 2 0.0 3 0 0 0 2 38 3 0.0 3 0 0 0 3 43 4 0.0 3 0 0 0 4 47 13 0.0 3 0 0 0 13 48 15 0.0 3 0 0 0 15 49 1 0.0 3 0 0 0 1 50 7 0.0 3 0 0 0 7 51 5 0.0 3 0 0 0 5 52 5 0.0 3 0 0 0 5 53 1 0.0 3 0 0 0 1 59 2 0.0 3 0 0 0 2 60 14 0.0 3 0 0 0 14 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 73 2 0.0 3 0 0 0 2 74 3 0.0 3 0 0 0 3 82 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 84 3 0.0 3 0 0 1 2 91 2 0.0 3 0 0 0 2 93 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 96 3 0.0 3 0 0 0 3 98 3 0.0 3 0 0 0 3 99 2 0.0 3 0 0 0 2 101 1 0.0 3 0 0 0 1 103 2 0.0 3 0 0 0 2 108 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 122 2 0.0 3 1 0 0 1 125 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 144 2 0.0 3 0 0 0 2 149 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 162 4 0.0 3 0 0 0 4 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 1 170 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 1 238 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 246 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.22 s (36 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 6,187 Reads with adapters: 177 (2.9%) Reads written (passing filters): 6,187 (100.0%) Total basepairs processed: 1,686,113 bp Total written (filtered): 1,685,577 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 177 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 53.7% C: 26.6% G: 14.1% T: 5.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 173 96.7 0 173 4 3 24.2 0 3 5 1 6.0 0 1