This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCGGTT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.06 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 380,120 (14.3%) Reads that were too short: 352,634 (13.3%) Reads written (passing filters): 27,486 (1.0%) Total basepairs processed: 800,111,879 bp Total written (filtered): 5,508,177 bp (0.7%) === Adapter 1 === Sequence: GCGGTT; Type: regular 5'; Length: 6; Trimmed: 380120 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 364 649.0 0 364 7 3119 649.0 0 3119 8 2634 649.0 0 2634 9 2530 649.0 0 2530 10 53 649.0 0 53 11 49 649.0 0 49 12 37 649.0 0 37 13 50 649.0 0 50 14 52 649.0 0 52 15 33 649.0 0 33 16 54 649.0 0 54 17 59 649.0 0 59 18 30 649.0 0 30 19 53 649.0 0 53 20 74 649.0 0 74 21 51 649.0 0 51 22 53 649.0 0 53 23 41 649.0 0 41 24 47 649.0 0 47 25 42 649.0 0 42 26 40 649.0 0 40 27 37 649.0 0 37 28 37 649.0 0 37 29 54 649.0 0 54 30 39 649.0 0 39 31 33 649.0 0 33 32 17 649.0 0 17 33 42 649.0 0 42 34 36 649.0 0 36 35 44 649.0 0 44 36 54 649.0 0 54 37 37 649.0 0 37 38 42 649.0 0 42 39 36 649.0 0 36 40 35 649.0 0 35 41 35 649.0 0 35 42 41 649.0 0 41 43 30 649.0 0 30 44 33 649.0 0 33 45 33 649.0 0 33 46 48 649.0 0 48 47 59 649.0 0 59 48 53 649.0 0 53 49 33 649.0 0 33 50 45 649.0 0 45 51 49 649.0 0 49 52 35 649.0 0 35 53 61 649.0 0 61 54 37 649.0 0 37 55 45 649.0 0 45 56 46 649.0 0 46 57 55 649.0 0 55 58 38 649.0 0 38 59 35 649.0 0 35 60 42 649.0 0 42 61 52 649.0 0 52 62 41 649.0 0 41 63 66 649.0 0 66 64 58 649.0 0 58 65 35 649.0 0 35 66 63 649.0 0 63 67 64 649.0 0 64 68 39 649.0 0 39 69 53 649.0 0 53 70 30 649.0 0 30 71 54 649.0 0 54 72 27 649.0 0 27 73 52 649.0 0 52 74 47 649.0 0 47 75 58 649.0 0 58 76 44 649.0 0 44 77 54 649.0 0 54 78 64 649.0 0 64 79 61 649.0 0 61 80 37 649.0 0 37 81 71 649.0 0 71 82 60 649.0 0 60 83 44 649.0 0 44 84 37 649.0 0 37 85 52 649.0 0 52 86 52 649.0 0 52 87 49 649.0 0 49 88 63 649.0 0 63 89 71 649.0 0 71 90 68 649.0 0 68 91 62 649.0 0 62 92 56 649.0 0 56 93 67 649.0 0 67 94 75 649.0 0 75 95 39 649.0 0 39 96 42 649.0 0 42 97 56 649.0 0 56 98 52 649.0 0 52 99 62 649.0 0 62 100 32 649.0 0 32 101 44 649.0 0 44 102 81 649.0 0 81 103 45 649.0 0 45 104 54 649.0 0 54 105 49 649.0 0 49 106 59 649.0 0 59 107 44 649.0 0 44 108 49 649.0 0 49 109 38 649.0 0 38 110 46 649.0 0 46 111 48 649.0 0 48 112 41 649.0 0 41 113 41 649.0 0 41 114 43 649.0 0 43 115 53 649.0 0 53 116 46 649.0 0 46 117 60 649.0 0 60 118 50 649.0 0 50 119 42 649.0 0 42 120 45 649.0 0 45 121 69 649.0 0 69 122 67 649.0 0 67 123 53 649.0 0 53 124 83 649.0 0 83 125 184 649.0 0 184 126 211 649.0 0 211 127 116 649.0 0 116 128 62 649.0 0 62 129 58 649.0 0 58 130 84 649.0 0 84 131 318 649.0 0 318 132 265 649.0 0 265 133 202 649.0 0 202 134 104 649.0 0 104 135 62 649.0 0 62 136 70 649.0 0 70 137 69 649.0 0 69 138 66 649.0 0 66 139 56 649.0 0 56 140 59 649.0 0 59 141 136 649.0 0 136 142 59 649.0 0 59 143 105 649.0 0 105 144 67 649.0 0 67 145 95 649.0 0 95 146 56 649.0 0 56 147 75 649.0 0 75 148 72 649.0 0 72 149 68 649.0 0 68 150 73 649.0 0 73 151 87 649.0 0 87 152 76 649.0 0 76 153 97 649.0 0 97 154 90 649.0 0 90 155 106 649.0 0 106 156 84 649.0 0 84 157 89 649.0 0 89 158 64 649.0 0 64 159 68 649.0 0 68 160 75 649.0 0 75 161 72 649.0 0 72 162 72 649.0 0 72 163 83 649.0 0 83 164 80 649.0 0 80 165 81 649.0 0 81 166 95 649.0 0 95 167 128 649.0 0 128 168 125 649.0 0 125 169 95 649.0 0 95 170 86 649.0 0 86 171 77 649.0 0 77 172 128 649.0 0 128 173 211 649.0 0 211 174 424 649.0 0 424 175 259 649.0 0 259 176 144 649.0 0 144 177 86 649.0 0 86 178 84 649.0 0 84 179 161 649.0 0 161 180 169 649.0 0 169 181 204 649.0 0 204 182 140 649.0 0 140 183 139 649.0 0 139 184 102 649.0 0 102 185 261 649.0 0 261 186 246 649.0 0 246 187 255 649.0 0 255 188 120 649.0 0 120 189 128 649.0 0 128 190 132 649.0 0 132 191 141 649.0 0 141 192 421 649.0 0 421 193 1617 649.0 0 1617 194 765 649.0 0 765 195 750 649.0 0 750 196 286 649.0 0 286 197 117 649.0 0 117 198 120 649.0 0 120 199 320 649.0 0 320 200 370 649.0 0 370 201 344 649.0 0 344 202 255 649.0 0 255 203 120 649.0 0 120 204 98 649.0 0 98 205 140 649.0 0 140 206 160 649.0 0 160 207 174 649.0 0 174 208 144 649.0 0 144 209 125 649.0 0 125 210 126 649.0 0 126 211 134 649.0 0 134 212 147 649.0 0 147 213 206 649.0 0 206 214 161 649.0 0 161 215 208 649.0 0 208 216 195 649.0 0 195 217 175 649.0 0 175 218 245 649.0 0 245 219 442 649.0 0 442 220 284 649.0 0 284 221 273 649.0 0 273 222 443 649.0 0 443 223 390 649.0 0 390 224 586 649.0 0 586 225 424 649.0 0 424 226 425 649.0 0 425 227 642 649.0 0 642 228 541 649.0 0 541 229 697 649.0 0 697 230 813 649.0 0 813 231 1597 649.0 0 1597 232 19734 649.0 0 19734 233 8935 649.0 0 8935 234 18990 649.0 0 18990 235 1174 649.0 0 1174 236 1573 649.0 0 1573 237 25743 649.0 0 25743 238 17136 649.0 0 17136 239 24996 649.0 0 24996 240 1442 649.0 0 1442 241 5043 649.0 0 5043 242 803 649.0 0 803 243 2437 649.0 0 2437 244 1319 649.0 0 1319 245 2105 649.0 0 2105 246 580 649.0 0 580 247 836 649.0 0 836 248 802 649.0 0 802 249 1407 649.0 0 1407 250 1714 649.0 0 1714 251 2658 649.0 0 2658 252 12656 649.0 0 12656 253 4183 649.0 0 4183 254 5355 649.0 0 5355 255 1543 649.0 0 1543 256 2560 649.0 0 2560 257 44897 649.0 0 44897 258 36577 649.0 0 36577 259 61743 649.0 0 61743 260 26469 649.0 0 26469 261 180 649.0 0 180 262 110 649.0 0 110 263 84 649.0 0 84 264 104 649.0 0 104 265 89 649.0 0 89 266 118 649.0 0 118 267 126 649.0 0 126 268 178 649.0 0 178 269 100 649.0 0 100 270 147 649.0 0 147 271 119 649.0 0 119 272 107 649.0 0 107 273 88 649.0 0 88 274 98 649.0 0 98 275 87 649.0 0 87 276 87 649.0 0 87 277 106 649.0 0 106 278 95 649.0 0 95 279 104 649.0 0 104 280 109 649.0 0 109 281 125 649.0 0 125 282 155 649.0 0 155 283 135 649.0 0 135 284 108 649.0 0 108 285 118 649.0 0 118 286 113 649.0 0 113 287 94 649.0 0 94 288 104 649.0 0 104 289 194 649.0 0 194 290 283 649.0 0 283 291 634 649.0 0 634 292 783 649.0 0 783 293 493 649.0 0 493 294 306 649.0 0 306 295 167 649.0 0 167 296 141 649.0 0 141 297 120 649.0 0 120 298 114 649.0 0 114 299 107 649.0 0 107 300 127 649.0 0 127 301 197 649.0 0 197 Finished in 1.20 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 27,486 Reads with adapters: 2,750 (10.0%) Reads that were too short: 15 (0.1%) Reads written (passing filters): 2,735 (10.0%) Total basepairs processed: 5,508,177 bp Total written (filtered): 740,284 bp (13.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 2750 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 16 0.0 3 0 0 0 0 16 18 5 0.0 3 0 0 5 19 63 0.0 3 0 59 4 20 2601 0.0 4 2536 62 2 1 21 7 0.0 4 1 5 0 0 1 22 3 0.0 4 3 23 4 0.0 4 0 0 0 3 1 27 1 0.0 4 0 1 40 1 0.0 4 0 0 0 0 1 42 1 0.0 4 0 0 0 0 1 43 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 129 32 0.0 4 0 0 0 0 32 130 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 231 1 0.0 4 0 0 0 0 1 250 2 0.0 4 0 2 251 2 0.0 4 1 0 0 0 1 252 1 0.0 4 0 1 261 1 0.0 4 0 0 0 0 1 269 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.09 s (33 us/read; 1.82 M reads/minute). === Summary === Total reads processed: 2,735 Reads with adapters: 310 (11.3%) Reads written (passing filters): 2,735 (100.0%) Total basepairs processed: 740,284 bp Total written (filtered): 736,248 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 310 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.6% C: 3.9% G: 92.9% T: 0.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 42.7 0 1 9 105 0.0 0 4 101 10 172 0.0 1 5 167 11 26 0.0 1 1 25 18 1 0.0 1 1 172 1 0.0 1 1 223 4 0.0 1 4 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.