This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 69.96 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 61,764 (2.3%) Reads that were too short: 9,730 (0.4%) Reads written (passing filters): 52,034 (2.0%) Total basepairs processed: 800,111,879 bp Total written (filtered): 13,104,323 bp (1.6%) === Adapter 1 === Sequence: GTAGTG; Type: regular 5'; Length: 6; Trimmed: 61764 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 497 649.0 0 497 7 17929 649.0 0 17929 8 315 649.0 0 315 9 14803 649.0 0 14803 10 37 649.0 0 37 11 15 649.0 0 15 12 15 649.0 0 15 13 26 649.0 0 26 14 13 649.0 0 13 15 16 649.0 0 16 16 36 649.0 0 36 17 15 649.0 0 15 18 17 649.0 0 17 19 13 649.0 0 13 20 27 649.0 0 27 21 11 649.0 0 11 22 24 649.0 0 24 23 26 649.0 0 26 24 19 649.0 0 19 25 33 649.0 0 33 26 104 649.0 0 104 27 34 649.0 0 34 28 131 649.0 0 131 29 81 649.0 0 81 30 46 649.0 0 46 31 95 649.0 0 95 32 16 649.0 0 16 33 25 649.0 0 25 34 30 649.0 0 30 35 28 649.0 0 28 36 24 649.0 0 24 37 22 649.0 0 22 38 35 649.0 0 35 39 24 649.0 0 24 40 17 649.0 0 17 41 12 649.0 0 12 42 39 649.0 0 39 43 25 649.0 0 25 44 36 649.0 0 36 45 20 649.0 0 20 46 21 649.0 0 21 47 19 649.0 0 19 48 13 649.0 0 13 49 38 649.0 0 38 50 22 649.0 0 22 51 37 649.0 0 37 52 39 649.0 0 39 53 27 649.0 0 27 54 21 649.0 0 21 55 38 649.0 0 38 56 42 649.0 0 42 57 27 649.0 0 27 58 29 649.0 0 29 59 31 649.0 0 31 60 26 649.0 0 26 61 17 649.0 0 17 62 30 649.0 0 30 63 38 649.0 0 38 64 119 649.0 0 119 65 37 649.0 0 37 66 29 649.0 0 29 67 51 649.0 0 51 68 79 649.0 0 79 69 762 649.0 0 762 70 261 649.0 0 261 71 655 649.0 0 655 72 50 649.0 0 50 73 50 649.0 0 50 74 20 649.0 0 20 75 40 649.0 0 40 76 35 649.0 0 35 77 145 649.0 0 145 78 36 649.0 0 36 79 103 649.0 0 103 80 30 649.0 0 30 81 24 649.0 0 24 82 51 649.0 0 51 83 52 649.0 0 52 84 33 649.0 0 33 85 22 649.0 0 22 86 35 649.0 0 35 87 35 649.0 0 35 88 44 649.0 0 44 89 42 649.0 0 42 90 57 649.0 0 57 91 37 649.0 0 37 92 39 649.0 0 39 93 48 649.0 0 48 94 59 649.0 0 59 95 34 649.0 0 34 96 69 649.0 0 69 97 98 649.0 0 98 98 48 649.0 0 48 99 64 649.0 0 64 100 50 649.0 0 50 101 60 649.0 0 60 102 39 649.0 0 39 103 23 649.0 0 23 104 41 649.0 0 41 105 32 649.0 0 32 106 19 649.0 0 19 107 30 649.0 0 30 108 23 649.0 0 23 109 37 649.0 0 37 110 75 649.0 0 75 111 41 649.0 0 41 112 83 649.0 0 83 113 47 649.0 0 47 114 132 649.0 0 132 115 235 649.0 0 235 116 248 649.0 0 248 117 26 649.0 0 26 118 36 649.0 0 36 119 15 649.0 0 15 120 27 649.0 0 27 121 31 649.0 0 31 122 26 649.0 0 26 123 41 649.0 0 41 124 38 649.0 0 38 125 43 649.0 0 43 126 46 649.0 0 46 127 35 649.0 0 35 128 63 649.0 0 63 129 44 649.0 0 44 130 44 649.0 0 44 131 46 649.0 0 46 132 66 649.0 0 66 133 74 649.0 0 74 134 124 649.0 0 124 135 87 649.0 0 87 136 77 649.0 0 77 137 215 649.0 0 215 138 289 649.0 0 289 139 1178 649.0 0 1178 140 494 649.0 0 494 141 117 649.0 0 117 142 244 649.0 0 244 143 384 649.0 0 384 144 128 649.0 0 128 145 71 649.0 0 71 146 81 649.0 0 81 147 98 649.0 0 98 148 1118 649.0 0 1118 149 1494 649.0 0 1494 150 1183 649.0 0 1183 151 1387 649.0 0 1387 152 255 649.0 0 255 153 70 649.0 0 70 154 75 649.0 0 75 155 58 649.0 0 58 156 91 649.0 0 91 157 34 649.0 0 34 158 67 649.0 0 67 159 46 649.0 0 46 160 115 649.0 0 115 161 32 649.0 0 32 162 26 649.0 0 26 163 29 649.0 0 29 164 18 649.0 0 18 165 24 649.0 0 24 166 23 649.0 0 23 167 39 649.0 0 39 168 32 649.0 0 32 169 33 649.0 0 33 170 26 649.0 0 26 171 26 649.0 0 26 172 44 649.0 0 44 173 92 649.0 0 92 174 45 649.0 0 45 175 29 649.0 0 29 176 30 649.0 0 30 177 38 649.0 0 38 178 26 649.0 0 26 179 53 649.0 0 53 180 41 649.0 0 41 181 46 649.0 0 46 182 19 649.0 0 19 183 58 649.0 0 58 184 212 649.0 0 212 185 64 649.0 0 64 186 95 649.0 0 95 187 28 649.0 0 28 188 40 649.0 0 40 189 64 649.0 0 64 190 58 649.0 0 58 191 106 649.0 0 106 192 58 649.0 0 58 193 59 649.0 0 59 194 50 649.0 0 50 195 35 649.0 0 35 196 42 649.0 0 42 197 33 649.0 0 33 198 36 649.0 0 36 199 32 649.0 0 32 200 32 649.0 0 32 201 26 649.0 0 26 202 36 649.0 0 36 203 37 649.0 0 37 204 31 649.0 0 31 205 31 649.0 0 31 206 43 649.0 0 43 207 44 649.0 0 44 208 46 649.0 0 46 209 79 649.0 0 79 210 78 649.0 0 78 211 71 649.0 0 71 212 43 649.0 0 43 213 36 649.0 0 36 214 41 649.0 0 41 215 44 649.0 0 44 216 40 649.0 0 40 217 35 649.0 0 35 218 23 649.0 0 23 219 36 649.0 0 36 220 40 649.0 0 40 221 28 649.0 0 28 222 48 649.0 0 48 223 38 649.0 0 38 224 26 649.0 0 26 225 28 649.0 0 28 226 27 649.0 0 27 227 27 649.0 0 27 228 39 649.0 0 39 229 24 649.0 0 24 230 34 649.0 0 34 231 30 649.0 0 30 232 41 649.0 0 41 233 103 649.0 0 103 234 112 649.0 0 112 235 142 649.0 0 142 236 68 649.0 0 68 237 64 649.0 0 64 238 59 649.0 0 59 239 59 649.0 0 59 240 64 649.0 0 64 241 46 649.0 0 46 242 41 649.0 0 41 243 79 649.0 0 79 244 69 649.0 0 69 245 64 649.0 0 64 246 45 649.0 0 45 247 90 649.0 0 90 248 94 649.0 0 94 249 92 649.0 0 92 250 84 649.0 0 84 251 65 649.0 0 65 252 64 649.0 0 64 253 271 649.0 0 271 254 192 649.0 0 192 255 377 649.0 0 377 256 62 649.0 0 62 257 42 649.0 0 42 258 58 649.0 0 58 259 43 649.0 0 43 260 49 649.0 0 49 261 49 649.0 0 49 262 53 649.0 0 53 263 33 649.0 0 33 264 53 649.0 0 53 265 38 649.0 0 38 266 47 649.0 0 47 267 46 649.0 0 46 268 240 649.0 0 240 269 81 649.0 0 81 270 107 649.0 0 107 271 53 649.0 0 53 272 90 649.0 0 90 273 177 649.0 0 177 274 180 649.0 0 180 275 126 649.0 0 126 276 89 649.0 0 89 277 48 649.0 0 48 278 94 649.0 0 94 279 81 649.0 0 81 280 133 649.0 0 133 281 84 649.0 0 84 282 59 649.0 0 59 283 63 649.0 0 63 284 68 649.0 0 68 285 87 649.0 0 87 286 96 649.0 0 96 287 96 649.0 0 96 288 154 649.0 0 154 289 307 649.0 0 307 290 114 649.0 0 114 291 98 649.0 0 98 292 205 649.0 0 205 293 125 649.0 0 125 294 134 649.0 0 134 295 221 649.0 0 221 296 193 649.0 0 193 297 242 649.0 0 242 298 280 649.0 0 280 299 367 649.0 0 367 300 411 649.0 0 411 301 686 649.0 0 686 Finished in 2.66 s (51 us/read; 1.17 M reads/minute). === Summary === Total reads processed: 52,034 Reads with adapters: 18,532 (35.6%) Reads that were too short: 16 (0.0%) Reads written (passing filters): 18,516 (35.6%) Total basepairs processed: 13,104,323 bp Total written (filtered): 5,038,981 bp (38.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 18532 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 1 0.0 3 0 0 0 1 18 8 0.0 3 0 0 7 1 19 224 0.0 3 0 219 2 2 1 20 17850 0.0 4 17513 326 8 2 1 21 97 0.0 4 68 28 1 22 4 0.0 4 2 0 1 1 23 11 0.0 4 0 0 0 11 24 1 0.0 4 0 0 0 0 1 57 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 85 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 108 4 0.0 4 0 0 0 0 4 109 1 0.0 4 0 1 125 1 0.0 4 0 0 0 0 1 126 5 0.0 4 0 0 0 0 5 127 308 0.0 4 0 0 0 0 308 128 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 1 147 1 0.0 4 0 0 0 0 1 180 1 0.0 4 0 1 181 1 0.0 4 1 189 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 257 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.62 s (33 us/read; 1.79 M reads/minute). === Summary === Total reads processed: 18,516 Reads with adapters: 463 (2.5%) Reads written (passing filters): 18,516 (100.0%) Total basepairs processed: 5,038,981 bp Total written (filtered): 5,033,558 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 463 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 7.1% C: 4.5% G: 87.7% T: 0.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 29 289.3 0 29 4 2 72.3 0 2 9 1 0.1 0 1 10 4 0.0 1 0 4 11 402 0.0 1 2 400 12 20 0.0 1 0 20 15 2 0.0 1 0 2 152 1 0.0 1 0 1 210 1 0.0 1 1 225 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.