This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCAGAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 70.12 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 153,402 (5.8%) Reads that were too short: 21,149 (0.8%) Reads written (passing filters): 132,253 (5.0%) Total basepairs processed: 800,111,879 bp Total written (filtered): 36,845,410 bp (4.6%) === Adapter 1 === Sequence: TCAGAG; Type: regular 5'; Length: 6; Trimmed: 153402 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 5125 649.0 0 5125 7 1631 649.0 0 1631 8 58647 649.0 0 58647 9 48458 649.0 0 48458 10 104 649.0 0 104 11 11 649.0 0 11 12 6 649.0 0 6 13 7 649.0 0 7 14 4 649.0 0 4 15 8 649.0 0 8 16 5 649.0 0 5 17 8 649.0 0 8 18 13 649.0 0 13 19 5 649.0 0 5 20 7 649.0 0 7 21 8 649.0 0 8 22 4 649.0 0 4 23 1 649.0 0 1 24 6 649.0 0 6 25 14 649.0 0 14 26 28 649.0 0 28 27 39 649.0 0 39 28 31 649.0 0 31 29 8 649.0 0 8 30 8 649.0 0 8 31 19 649.0 0 19 32 37 649.0 0 37 33 22 649.0 0 22 34 22 649.0 0 22 35 15 649.0 0 15 36 11 649.0 0 11 37 9 649.0 0 9 38 22 649.0 0 22 39 32 649.0 0 32 40 49 649.0 0 49 41 36 649.0 0 36 42 44 649.0 0 44 43 49 649.0 0 49 44 21 649.0 0 21 45 13 649.0 0 13 46 24 649.0 0 24 47 17 649.0 0 17 48 26 649.0 0 26 49 19 649.0 0 19 50 17 649.0 0 17 51 16 649.0 0 16 52 56 649.0 0 56 53 37 649.0 0 37 54 23 649.0 0 23 55 19 649.0 0 19 56 25 649.0 0 25 57 38 649.0 0 38 58 191 649.0 0 191 59 224 649.0 0 224 60 1453 649.0 0 1453 61 1178 649.0 0 1178 62 945 649.0 0 945 63 24 649.0 0 24 64 28 649.0 0 28 65 30 649.0 0 30 66 267 649.0 0 267 67 163 649.0 0 163 68 88 649.0 0 88 69 17 649.0 0 17 70 24 649.0 0 24 71 26 649.0 0 26 72 27 649.0 0 27 73 25 649.0 0 25 74 23 649.0 0 23 75 27 649.0 0 27 76 56 649.0 0 56 77 287 649.0 0 287 78 121 649.0 0 121 79 155 649.0 0 155 80 21 649.0 0 21 81 75 649.0 0 75 82 101 649.0 0 101 83 61 649.0 0 61 84 238 649.0 0 238 85 226 649.0 0 226 86 27 649.0 0 27 87 38 649.0 0 38 88 39 649.0 0 39 89 44 649.0 0 44 90 28 649.0 0 28 91 105 649.0 0 105 92 43 649.0 0 43 93 113 649.0 0 113 94 89 649.0 0 89 95 53 649.0 0 53 96 32 649.0 0 32 97 64 649.0 0 64 98 53 649.0 0 53 99 42 649.0 0 42 100 28 649.0 0 28 101 19 649.0 0 19 102 19 649.0 0 19 103 22 649.0 0 22 104 40 649.0 0 40 105 25 649.0 0 25 106 25 649.0 0 25 107 39 649.0 0 39 108 54 649.0 0 54 109 33 649.0 0 33 110 27 649.0 0 27 111 225 649.0 0 225 112 114 649.0 0 114 113 182 649.0 0 182 114 1055 649.0 0 1055 115 430 649.0 0 430 116 1681 649.0 0 1681 117 179 649.0 0 179 118 115 649.0 0 115 119 48 649.0 0 48 120 30 649.0 0 30 121 26 649.0 0 26 122 74 649.0 0 74 123 68 649.0 0 68 124 64 649.0 0 64 125 55 649.0 0 55 126 112 649.0 0 112 127 50 649.0 0 50 128 35 649.0 0 35 129 45 649.0 0 45 130 53 649.0 0 53 131 70 649.0 0 70 132 43 649.0 0 43 133 67 649.0 0 67 134 29 649.0 0 29 135 51 649.0 0 51 136 61 649.0 0 61 137 83 649.0 0 83 138 46 649.0 0 46 139 91 649.0 0 91 140 68 649.0 0 68 141 49 649.0 0 49 142 34 649.0 0 34 143 104 649.0 0 104 144 89 649.0 0 89 145 182 649.0 0 182 146 39 649.0 0 39 147 69 649.0 0 69 148 186 649.0 0 186 149 65 649.0 0 65 150 60 649.0 0 60 151 36 649.0 0 36 152 41 649.0 0 41 153 37 649.0 0 37 154 44 649.0 0 44 155 36 649.0 0 36 156 63 649.0 0 63 157 48 649.0 0 48 158 36 649.0 0 36 159 52 649.0 0 52 160 57 649.0 0 57 161 82 649.0 0 82 162 50 649.0 0 50 163 65 649.0 0 65 164 72 649.0 0 72 165 168 649.0 0 168 166 110 649.0 0 110 167 152 649.0 0 152 168 41 649.0 0 41 169 64 649.0 0 64 170 55 649.0 0 55 171 65 649.0 0 65 172 46 649.0 0 46 173 46 649.0 0 46 174 42 649.0 0 42 175 37 649.0 0 37 176 62 649.0 0 62 177 59 649.0 0 59 178 61 649.0 0 61 179 172 649.0 0 172 180 127 649.0 0 127 181 121 649.0 0 121 182 54 649.0 0 54 183 50 649.0 0 50 184 101 649.0 0 101 185 121 649.0 0 121 186 92 649.0 0 92 187 64 649.0 0 64 188 74 649.0 0 74 189 168 649.0 0 168 190 139 649.0 0 139 191 287 649.0 0 287 192 166 649.0 0 166 193 92 649.0 0 92 194 55 649.0 0 55 195 60 649.0 0 60 196 63 649.0 0 63 197 61 649.0 0 61 198 75 649.0 0 75 199 48 649.0 0 48 200 54 649.0 0 54 201 49 649.0 0 49 202 48 649.0 0 48 203 170 649.0 0 170 204 155 649.0 0 155 205 116 649.0 0 116 206 56 649.0 0 56 207 86 649.0 0 86 208 175 649.0 0 175 209 98 649.0 0 98 210 148 649.0 0 148 211 53 649.0 0 53 212 143 649.0 0 143 213 91 649.0 0 91 214 112 649.0 0 112 215 69 649.0 0 69 216 117 649.0 0 117 217 147 649.0 0 147 218 142 649.0 0 142 219 81 649.0 0 81 220 61 649.0 0 61 221 63 649.0 0 63 222 67 649.0 0 67 223 69 649.0 0 69 224 122 649.0 0 122 225 176 649.0 0 176 226 136 649.0 0 136 227 87 649.0 0 87 228 74 649.0 0 74 229 81 649.0 0 81 230 51 649.0 0 51 231 96 649.0 0 96 232 115 649.0 0 115 233 109 649.0 0 109 234 107 649.0 0 107 235 145 649.0 0 145 236 340 649.0 0 340 237 470 649.0 0 470 238 357 649.0 0 357 239 280 649.0 0 280 240 79 649.0 0 79 241 83 649.0 0 83 242 125 649.0 0 125 243 117 649.0 0 117 244 240 649.0 0 240 245 3099 649.0 0 3099 246 1502 649.0 0 1502 247 3407 649.0 0 3407 248 115 649.0 0 115 249 72 649.0 0 72 250 64 649.0 0 64 251 69 649.0 0 69 252 65 649.0 0 65 253 83 649.0 0 83 254 124 649.0 0 124 255 93 649.0 0 93 256 89 649.0 0 89 257 68 649.0 0 68 258 83 649.0 0 83 259 105 649.0 0 105 260 195 649.0 0 195 261 306 649.0 0 306 262 167 649.0 0 167 263 135 649.0 0 135 264 149 649.0 0 149 265 512 649.0 0 512 266 334 649.0 0 334 267 291 649.0 0 291 268 98 649.0 0 98 269 68 649.0 0 68 270 251 649.0 0 251 271 220 649.0 0 220 272 356 649.0 0 356 273 143 649.0 0 143 274 66 649.0 0 66 275 123 649.0 0 123 276 147 649.0 0 147 277 127 649.0 0 127 278 121 649.0 0 121 279 178 649.0 0 178 280 142 649.0 0 142 281 129 649.0 0 129 282 104 649.0 0 104 283 71 649.0 0 71 284 97 649.0 0 97 285 122 649.0 0 122 286 151 649.0 0 151 287 109 649.0 0 109 288 87 649.0 0 87 289 71 649.0 0 71 290 73 649.0 0 73 291 82 649.0 0 82 292 101 649.0 0 101 293 135 649.0 0 135 294 140 649.0 0 140 295 97 649.0 0 97 296 111 649.0 0 111 297 134 649.0 0 134 298 147 649.0 0 147 299 122 649.0 0 122 300 153 649.0 0 153 301 89 649.0 0 89 Finished in 6.24 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 132,253 Reads with adapters: 58,656 (44.4%) Reads that were too short: 162 (0.1%) Reads written (passing filters): 58,494 (44.2%) Total basepairs processed: 36,845,410 bp Total written (filtered): 16,130,963 bp (43.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 58656 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 10 0.0 2 0 0 0 10 15 26 0.0 3 0 0 18 8 16 510 0.0 3 0 441 54 15 17 57459 0.0 3 54405 2546 426 82 18 146 0.0 3 11 106 11 18 19 120 0.0 3 4 0 73 43 20 121 0.0 3 0 0 0 121 21 1 0.0 3 0 0 0 1 22 1 0.0 3 0 0 0 1 23 1 0.0 3 0 0 0 1 24 3 0.0 3 2 0 0 1 26 4 0.0 3 1 0 0 3 28 4 0.0 3 1 0 0 3 29 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 31 2 0.0 3 0 0 0 2 33 1 0.0 3 0 0 0 1 37 2 0.0 3 0 0 0 2 38 1 0.0 3 0 0 0 1 44 2 0.0 3 0 0 0 2 45 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 52 1 0.0 3 0 1 53 2 0.0 3 0 0 0 2 54 2 0.0 3 1 0 0 1 55 1 0.0 3 0 0 0 1 57 3 0.0 3 0 0 0 3 58 1 0.0 3 0 0 1 59 1 0.0 3 0 0 0 1 60 3 0.0 3 0 0 0 3 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 1 68 1 0.0 3 0 0 1 73 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 77 10 0.0 3 0 0 0 10 78 13 0.0 3 0 0 0 13 80 3 0.0 3 1 0 0 2 81 7 0.0 3 0 0 0 7 83 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 88 2 0.0 3 0 0 0 2 90 1 0.0 3 0 0 0 1 102 2 0.0 3 0 0 0 2 103 3 0.0 3 0 0 0 3 104 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 107 2 0.0 3 0 0 0 2 109 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 116 3 0.0 3 0 0 0 3 119 1 0.0 3 0 0 0 1 120 2 0.0 3 0 0 0 2 121 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 128 3 0.0 3 0 0 0 3 134 4 0.0 3 0 0 0 4 136 3 0.0 3 1 0 0 2 137 2 0.0 3 0 0 0 2 138 2 0.0 3 0 0 0 2 139 3 0.0 3 0 0 0 3 140 1 0.0 3 0 0 0 1 141 3 0.0 3 0 0 2 1 143 1 0.0 3 0 0 0 1 148 41 0.0 3 0 0 0 41 152 1 0.0 3 0 0 1 154 3 0.0 3 0 0 0 3 155 1 0.0 3 0 0 0 1 156 2 0.0 3 0 0 0 2 157 2 0.0 3 0 0 0 2 158 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 169 2 0.0 3 0 0 0 2 170 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 173 2 0.0 3 0 0 0 2 177 1 0.0 3 0 0 0 1 181 2 0.0 3 0 0 0 2 182 4 0.0 3 0 0 0 4 183 2 0.0 3 0 0 0 2 184 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 187 2 0.0 3 0 0 0 2 190 1 0.0 3 0 0 0 1 192 5 0.0 3 0 0 0 5 193 1 0.0 3 0 0 0 1 194 4 0.0 3 0 0 0 4 196 1 0.0 3 0 0 1 201 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 1 221 1 0.0 3 0 0 0 1 226 1 0.0 3 0 0 0 1 227 2 0.0 3 0 0 1 1 228 1 0.0 3 0 0 0 1 229 2 0.0 3 0 0 0 2 233 1 0.0 3 0 0 0 1 234 2 0.0 3 0 0 0 2 235 1 0.0 3 0 0 0 1 238 2 0.0 3 0 0 0 2 239 1 0.0 3 0 0 0 1 269 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 279 7 0.0 3 0 0 0 7 280 4 0.0 3 0 0 0 4 281 7 0.0 3 0 0 0 7 282 7 0.0 3 0 0 1 6 283 1 0.0 3 0 0 0 1 284 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 291 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.10 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 58,494 Reads with adapters: 2,169 (3.7%) Reads written (passing filters): 58,494 (100.0%) Total basepairs processed: 16,130,963 bp Total written (filtered): 16,124,378 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 2169 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 69.2% C: 16.5% G: 11.1% T: 3.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2105 914.0 0 2105 4 50 228.5 0 50 5 14 57.1 0 14