This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCAGAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 71.38 s (27 us/read; 2.23 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 146,764 (5.5%) Reads that were too short: 15,741 (0.6%) Reads written (passing filters): 131,023 (4.9%) Total basepairs processed: 800,111,879 bp Total written (filtered): 36,956,871 bp (4.6%) === Adapter 1 === Sequence: TCAGAG; Type: regular 5'; Length: 6; Trimmed: 146764 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 5377 649.0 0 5377 7 1221 649.0 0 1221 8 52843 649.0 0 52843 9 58102 649.0 0 58102 10 51 649.0 0 51 11 5 649.0 0 5 12 2 649.0 0 2 13 5 649.0 0 5 14 4 649.0 0 4 15 1 649.0 0 1 16 4 649.0 0 4 17 3 649.0 0 3 18 3 649.0 0 3 19 3 649.0 0 3 20 2 649.0 0 2 21 2 649.0 0 2 22 4 649.0 0 4 23 4 649.0 0 4 24 4 649.0 0 4 25 3 649.0 0 3 26 5 649.0 0 5 27 13 649.0 0 13 28 5 649.0 0 5 29 3 649.0 0 3 30 4 649.0 0 4 31 18 649.0 0 18 32 50 649.0 0 50 33 18 649.0 0 18 34 21 649.0 0 21 35 25 649.0 0 25 36 12 649.0 0 12 37 7 649.0 0 7 38 21 649.0 0 21 39 40 649.0 0 40 40 48 649.0 0 48 41 36 649.0 0 36 42 39 649.0 0 39 43 33 649.0 0 33 44 20 649.0 0 20 45 28 649.0 0 28 46 31 649.0 0 31 47 21 649.0 0 21 48 23 649.0 0 23 49 24 649.0 0 24 50 16 649.0 0 16 51 17 649.0 0 17 52 31 649.0 0 31 53 19 649.0 0 19 54 19 649.0 0 19 55 17 649.0 0 17 56 26 649.0 0 26 57 31 649.0 0 31 58 201 649.0 0 201 59 144 649.0 0 144 60 784 649.0 0 784 61 642 649.0 0 642 62 803 649.0 0 803 63 16 649.0 0 16 64 12 649.0 0 12 65 18 649.0 0 18 66 235 649.0 0 235 67 180 649.0 0 180 68 61 649.0 0 61 69 15 649.0 0 15 70 26 649.0 0 26 71 37 649.0 0 37 72 19 649.0 0 19 73 18 649.0 0 18 74 17 649.0 0 17 75 11 649.0 0 11 76 27 649.0 0 27 77 216 649.0 0 216 78 93 649.0 0 93 79 98 649.0 0 98 80 15 649.0 0 15 81 61 649.0 0 61 82 86 649.0 0 86 83 37 649.0 0 37 84 57 649.0 0 57 85 182 649.0 0 182 86 25 649.0 0 25 87 11 649.0 0 11 88 34 649.0 0 34 89 59 649.0 0 59 90 32 649.0 0 32 91 34 649.0 0 34 92 46 649.0 0 46 93 61 649.0 0 61 94 42 649.0 0 42 95 39 649.0 0 39 96 33 649.0 0 33 97 52 649.0 0 52 98 67 649.0 0 67 99 39 649.0 0 39 100 36 649.0 0 36 101 26 649.0 0 26 102 25 649.0 0 25 103 34 649.0 0 34 104 39 649.0 0 39 105 27 649.0 0 27 106 20 649.0 0 20 107 35 649.0 0 35 108 50 649.0 0 50 109 38 649.0 0 38 110 30 649.0 0 30 111 172 649.0 0 172 112 93 649.0 0 93 113 131 649.0 0 131 114 767 649.0 0 767 115 223 649.0 0 223 116 1262 649.0 0 1262 117 177 649.0 0 177 118 103 649.0 0 103 119 31 649.0 0 31 120 21 649.0 0 21 121 19 649.0 0 19 122 48 649.0 0 48 123 83 649.0 0 83 124 60 649.0 0 60 125 38 649.0 0 38 126 110 649.0 0 110 127 49 649.0 0 49 128 28 649.0 0 28 129 32 649.0 0 32 130 38 649.0 0 38 131 48 649.0 0 48 132 35 649.0 0 35 133 43 649.0 0 43 134 20 649.0 0 20 135 31 649.0 0 31 136 42 649.0 0 42 137 56 649.0 0 56 138 35 649.0 0 35 139 101 649.0 0 101 140 75 649.0 0 75 141 37 649.0 0 37 142 24 649.0 0 24 143 89 649.0 0 89 144 43 649.0 0 43 145 180 649.0 0 180 146 39 649.0 0 39 147 31 649.0 0 31 148 233 649.0 0 233 149 46 649.0 0 46 150 28 649.0 0 28 151 33 649.0 0 33 152 28 649.0 0 28 153 27 649.0 0 27 154 35 649.0 0 35 155 35 649.0 0 35 156 68 649.0 0 68 157 21 649.0 0 21 158 24 649.0 0 24 159 27 649.0 0 27 160 23 649.0 0 23 161 63 649.0 0 63 162 28 649.0 0 28 163 26 649.0 0 26 164 38 649.0 0 38 165 115 649.0 0 115 166 74 649.0 0 74 167 95 649.0 0 95 168 32 649.0 0 32 169 72 649.0 0 72 170 45 649.0 0 45 171 42 649.0 0 42 172 18 649.0 0 18 173 30 649.0 0 30 174 29 649.0 0 29 175 34 649.0 0 34 176 51 649.0 0 51 177 69 649.0 0 69 178 37 649.0 0 37 179 154 649.0 0 154 180 67 649.0 0 67 181 121 649.0 0 121 182 34 649.0 0 34 183 31 649.0 0 31 184 51 649.0 0 51 185 68 649.0 0 68 186 51 649.0 0 51 187 37 649.0 0 37 188 57 649.0 0 57 189 96 649.0 0 96 190 112 649.0 0 112 191 218 649.0 0 218 192 155 649.0 0 155 193 104 649.0 0 104 194 44 649.0 0 44 195 34 649.0 0 34 196 35 649.0 0 35 197 37 649.0 0 37 198 49 649.0 0 49 199 34 649.0 0 34 200 50 649.0 0 50 201 35 649.0 0 35 202 48 649.0 0 48 203 141 649.0 0 141 204 175 649.0 0 175 205 93 649.0 0 93 206 30 649.0 0 30 207 42 649.0 0 42 208 79 649.0 0 79 209 36 649.0 0 36 210 71 649.0 0 71 211 31 649.0 0 31 212 119 649.0 0 119 213 56 649.0 0 56 214 98 649.0 0 98 215 34 649.0 0 34 216 61 649.0 0 61 217 74 649.0 0 74 218 73 649.0 0 73 219 54 649.0 0 54 220 39 649.0 0 39 221 42 649.0 0 42 222 33 649.0 0 33 223 40 649.0 0 40 224 71 649.0 0 71 225 82 649.0 0 82 226 65 649.0 0 65 227 52 649.0 0 52 228 43 649.0 0 43 229 51 649.0 0 51 230 44 649.0 0 44 231 60 649.0 0 60 232 94 649.0 0 94 233 51 649.0 0 51 234 80 649.0 0 80 235 100 649.0 0 100 236 206 649.0 0 206 237 246 649.0 0 246 238 204 649.0 0 204 239 183 649.0 0 183 240 73 649.0 0 73 241 73 649.0 0 73 242 98 649.0 0 98 243 98 649.0 0 98 244 159 649.0 0 159 245 2637 649.0 0 2637 246 1379 649.0 0 1379 247 3390 649.0 0 3390 248 93 649.0 0 93 249 50 649.0 0 50 250 44 649.0 0 44 251 29 649.0 0 29 252 40 649.0 0 40 253 46 649.0 0 46 254 48 649.0 0 48 255 55 649.0 0 55 256 55 649.0 0 55 257 39 649.0 0 39 258 39 649.0 0 39 259 60 649.0 0 60 260 175 649.0 0 175 261 221 649.0 0 221 262 115 649.0 0 115 263 99 649.0 0 99 264 83 649.0 0 83 265 346 649.0 0 346 266 241 649.0 0 241 267 182 649.0 0 182 268 42 649.0 0 42 269 61 649.0 0 61 270 115 649.0 0 115 271 111 649.0 0 111 272 139 649.0 0 139 273 65 649.0 0 65 274 40 649.0 0 40 275 122 649.0 0 122 276 131 649.0 0 131 277 96 649.0 0 96 278 64 649.0 0 64 279 88 649.0 0 88 280 68 649.0 0 68 281 51 649.0 0 51 282 43 649.0 0 43 283 35 649.0 0 35 284 55 649.0 0 55 285 50 649.0 0 50 286 93 649.0 0 93 287 67 649.0 0 67 288 77 649.0 0 77 289 43 649.0 0 43 290 67 649.0 0 67 291 81 649.0 0 81 292 68 649.0 0 68 293 112 649.0 0 112 294 117 649.0 0 117 295 79 649.0 0 79 296 76 649.0 0 76 297 108 649.0 0 108 298 74 649.0 0 74 299 92 649.0 0 92 300 77 649.0 0 77 301 66 649.0 0 66 Finished in 6.74 s (51 us/read; 1.17 M reads/minute). === Summary === Total reads processed: 131,023 Reads with adapters: 67,527 (51.5%) Reads that were too short: 17 (0.0%) Reads written (passing filters): 67,510 (51.5%) Total basepairs processed: 36,956,871 bp Total written (filtered): 18,121,884 bp (49.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 67527 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 0 2 18 21 0.0 3 0 0 20 0 1 19 811 0.0 3 0 801 10 20 59417 0.0 4 58259 1112 43 3 21 191 0.0 4 40 146 5 22 4647 0.0 4 4559 74 13 0 1 23 63 0.0 4 6 9 0 39 9 24 5 0.0 4 1 0 3 1 25 4 0.0 4 0 0 0 4 30 1 0.0 4 1 42 2 0.0 4 1 0 0 0 1 46 1 0.0 4 1 94 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 3 0.0 4 0 0 0 0 3 126 27 0.0 4 0 0 0 0 27 127 2304 0.0 4 0 0 0 0 2304 128 4 0.0 4 0 0 0 0 4 130 1 0.0 4 0 0 0 0 1 135 1 0.0 4 1 140 1 0.0 4 0 1 146 1 0.0 4 0 1 153 2 0.0 4 0 0 0 0 2 181 1 0.0 4 0 0 0 0 1 201 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 229 2 0.0 4 0 0 0 0 2 234 1 0.0 4 0 0 0 0 1 249 2 0.0 4 2 262 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.40 s (36 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 67,510 Reads with adapters: 11,943 (17.7%) Reads written (passing filters): 67,510 (100.0%) Total basepairs processed: 18,121,884 bp Total written (filtered): 18,002,937 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 11943 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.0% C: 2.2% G: 95.3% T: 1.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 17 1054.8 0 17 4 12 263.7 0 12 5 4 65.9 0 4 6 7 16.5 0 7 9 7546 0.3 0 226 7320 10 3574 0.1 1 114 3460 11 722 0.0 1 10 712 12 24 0.0 1 3 21 13 5 0.0 1 0 5 168 1 0.0 1 1 169 2 0.0 1 1 1 171 1 0.0 1 1 208 5 0.0 1 3 2 209 1 0.0 1 0 1 223 19 0.0 1 18 1 224 2 0.0 1 2 226 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.