This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 57.64 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 146,125 (6.5%) Reads that were too short: 78,521 (3.5%) Reads written (passing filters): 67,604 (3.0%) Total basepairs processed: 673,916,425 bp Total written (filtered): 17,326,506 bp (2.6%) === Adapter 1 === Sequence: ACAACC; Type: regular 5'; Length: 6; Trimmed: 146125 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 516 546.6 0 516 7 26833 546.6 0 26833 8 19787 546.6 0 19787 9 46 546.6 0 46 10 41 546.6 0 41 11 99 546.6 0 99 12 27 546.6 0 27 13 30 546.6 0 30 14 33 546.6 0 33 15 52 546.6 0 52 16 45 546.6 0 45 17 47 546.6 0 47 18 31 546.6 0 31 19 33 546.6 0 33 20 42 546.6 0 42 21 28 546.6 0 28 22 24 546.6 0 24 23 31 546.6 0 31 24 38 546.6 0 38 25 34 546.6 0 34 26 26 546.6 0 26 27 38 546.6 0 38 28 23 546.6 0 23 29 33 546.6 0 33 30 38 546.6 0 38 31 41 546.6 0 41 32 32 546.6 0 32 33 45 546.6 0 45 34 51 546.6 0 51 35 40 546.6 0 40 36 45 546.6 0 45 37 29 546.6 0 29 38 39 546.6 0 39 39 46 546.6 0 46 40 43 546.6 0 43 41 60 546.6 0 60 42 34 546.6 0 34 43 47 546.6 0 47 44 41 546.6 0 41 45 48 546.6 0 48 46 28 546.6 0 28 47 52 546.6 0 52 48 40 546.6 0 40 49 32 546.6 0 32 50 30 546.6 0 30 51 39 546.6 0 39 52 47 546.6 0 47 53 95 546.6 0 95 54 125 546.6 0 125 55 73 546.6 0 73 56 47 546.6 0 47 57 56 546.6 0 56 58 41 546.6 0 41 59 151 546.6 0 151 60 52 546.6 0 52 61 51 546.6 0 51 62 41 546.6 0 41 63 50 546.6 0 50 64 68 546.6 0 68 65 57 546.6 0 57 66 53 546.6 0 53 67 60 546.6 0 60 68 70 546.6 0 70 69 70 546.6 0 70 70 38 546.6 0 38 71 67 546.6 0 67 72 199 546.6 0 199 73 127 546.6 0 127 74 209 546.6 0 209 75 45 546.6 0 45 76 57 546.6 0 57 77 51 546.6 0 51 78 61 546.6 0 61 79 66 546.6 0 66 80 75 546.6 0 75 81 77 546.6 0 77 82 83 546.6 0 83 83 66 546.6 0 66 84 58 546.6 0 58 85 62 546.6 0 62 86 76 546.6 0 76 87 48 546.6 0 48 88 61 546.6 0 61 89 49 546.6 0 49 90 37 546.6 0 37 91 49 546.6 0 49 92 48 546.6 0 48 93 59 546.6 0 59 94 56 546.6 0 56 95 50 546.6 0 50 96 62 546.6 0 62 97 37 546.6 0 37 98 45 546.6 0 45 99 62 546.6 0 62 100 61 546.6 0 61 101 52 546.6 0 52 102 93 546.6 0 93 103 140 546.6 0 140 104 193 546.6 0 193 105 110 546.6 0 110 106 86 546.6 0 86 107 85 546.6 0 85 108 68 546.6 0 68 109 79 546.6 0 79 110 68 546.6 0 68 111 78 546.6 0 78 112 72 546.6 0 72 113 61 546.6 0 61 114 73 546.6 0 73 115 74 546.6 0 74 116 91 546.6 0 91 117 88 546.6 0 88 118 73 546.6 0 73 119 79 546.6 0 79 120 87 546.6 0 87 121 109 546.6 0 109 122 137 546.6 0 137 123 134 546.6 0 134 124 464 546.6 0 464 125 364 546.6 0 364 126 860 546.6 0 860 127 96 546.6 0 96 128 108 546.6 0 108 129 87 546.6 0 87 130 89 546.6 0 89 131 86 546.6 0 86 132 143 546.6 0 143 133 200 546.6 0 200 134 162 546.6 0 162 135 119 546.6 0 119 136 141 546.6 0 141 137 106 546.6 0 106 138 99 546.6 0 99 139 109 546.6 0 109 140 98 546.6 0 98 141 90 546.6 0 90 142 77 546.6 0 77 143 87 546.6 0 87 144 96 546.6 0 96 145 104 546.6 0 104 146 177 546.6 0 177 147 295 546.6 0 295 148 158 546.6 0 158 149 234 546.6 0 234 150 154 546.6 0 154 151 103 546.6 0 103 152 123 546.6 0 123 153 130 546.6 0 130 154 117 546.6 0 117 155 121 546.6 0 121 156 197 546.6 0 197 157 147 546.6 0 147 158 151 546.6 0 151 159 110 546.6 0 110 160 142 546.6 0 142 161 144 546.6 0 144 162 195 546.6 0 195 163 202 546.6 0 202 164 135 546.6 0 135 165 116 546.6 0 116 166 123 546.6 0 123 167 160 546.6 0 160 168 151 546.6 0 151 169 408 546.6 0 408 170 464 546.6 0 464 171 511 546.6 0 511 172 248 546.6 0 248 173 221 546.6 0 221 174 146 546.6 0 146 175 134 546.6 0 134 176 107 546.6 0 107 177 93 546.6 0 93 178 103 546.6 0 103 179 129 546.6 0 129 180 176 546.6 0 176 181 183 546.6 0 183 182 241 546.6 0 241 183 172 546.6 0 172 184 151 546.6 0 151 185 184 546.6 0 184 186 137 546.6 0 137 187 231 546.6 0 231 188 156 546.6 0 156 189 171 546.6 0 171 190 128 546.6 0 128 191 95 546.6 0 95 192 134 546.6 0 134 193 151 546.6 0 151 194 144 546.6 0 144 195 176 546.6 0 176 196 88 546.6 0 88 197 119 546.6 0 119 198 116 546.6 0 116 199 139 546.6 0 139 200 146 546.6 0 146 201 117 546.6 0 117 202 138 546.6 0 138 203 256 546.6 0 256 204 189 546.6 0 189 205 288 546.6 0 288 206 117 546.6 0 117 207 89 546.6 0 89 208 88 546.6 0 88 209 122 546.6 0 122 210 111 546.6 0 111 211 136 546.6 0 136 212 130 546.6 0 130 213 307 546.6 0 307 214 1100 546.6 0 1100 215 2872 546.6 0 2872 216 1625 546.6 0 1625 217 565 546.6 0 565 218 266 546.6 0 266 219 308 546.6 0 308 220 147 546.6 0 147 221 162 546.6 0 162 222 164 546.6 0 164 223 263 546.6 0 263 224 288 546.6 0 288 225 779 546.6 0 779 226 3510 546.6 0 3510 227 12016 546.6 0 12016 228 5423 546.6 0 5423 229 4295 546.6 0 4295 230 1087 546.6 0 1087 231 1978 546.6 0 1978 232 995 546.6 0 995 233 430 546.6 0 430 234 116 546.6 0 116 235 114 546.6 0 114 236 142 546.6 0 142 237 405 546.6 0 405 238 303 546.6 0 303 239 336 546.6 0 336 240 160 546.6 0 160 241 125 546.6 0 125 242 105 546.6 0 105 243 92 546.6 0 92 244 133 546.6 0 133 245 151 546.6 0 151 246 148 546.6 0 148 247 147 546.6 0 147 248 224 546.6 0 224 249 207 546.6 0 207 250 171 546.6 0 171 251 96 546.6 0 96 252 107 546.6 0 107 253 109 546.6 0 109 254 148 546.6 0 148 255 184 546.6 0 184 256 242 546.6 0 242 257 379 546.6 0 379 258 269 546.6 0 269 259 292 546.6 0 292 260 246 546.6 0 246 261 483 546.6 0 483 262 296 546.6 0 296 263 488 546.6 0 488 264 2376 546.6 0 2376 265 3093 546.6 0 3093 266 4111 546.6 0 4111 267 2557 546.6 0 2557 268 1692 546.6 0 1692 269 752 546.6 0 752 270 1071 546.6 0 1071 271 268 546.6 0 268 272 266 546.6 0 266 273 483 546.6 0 483 274 637 546.6 0 637 275 521 546.6 0 521 276 411 546.6 0 411 277 179 546.6 0 179 278 285 546.6 0 285 279 357 546.6 0 357 280 463 546.6 0 463 281 491 546.6 0 491 282 245 546.6 0 245 283 159 546.6 0 159 284 219 546.6 0 219 285 236 546.6 0 236 286 311 546.6 0 311 287 363 546.6 0 363 288 297 546.6 0 297 289 243 546.6 0 243 290 297 546.6 0 297 291 2212 546.6 0 2212 292 1731 546.6 0 1731 293 3831 546.6 0 3831 294 444 546.6 0 444 295 143 546.6 0 143 296 141 546.6 0 141 297 189 546.6 0 189 298 144 546.6 0 144 299 169 546.6 0 169 300 159 546.6 0 159 301 313 546.6 0 313 Finished in 3.00 s (44 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 67,604 Reads with adapters: 26,755 (39.6%) Reads that were too short: 71 (0.1%) Reads written (passing filters): 26,684 (39.5%) Total basepairs processed: 17,326,506 bp Total written (filtered): 7,372,757 bp (42.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 26755 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 7 0.0 3 0 0 6 1 16 242 0.0 3 0 204 29 9 17 26051 0.0 3 24715 1090 193 53 18 60 0.0 3 3 46 8 3 19 32 0.0 3 0 0 9 23 20 181 0.0 3 0 0 0 181 21 1 0.0 3 0 0 0 1 22 5 0.0 3 0 0 0 5 23 4 0.0 3 0 0 0 4 24 1 0.0 3 0 0 0 1 25 2 0.0 3 1 0 0 1 26 5 0.0 3 1 0 1 3 30 4 0.0 3 0 0 0 4 33 3 0.0 3 0 0 0 3 35 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 46 2 0.0 3 0 0 1 1 49 2 0.0 3 0 0 0 2 50 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 1 64 1 0.0 3 0 0 0 1 67 40 0.0 3 0 0 0 40 69 1 0.0 3 0 0 0 1 70 8 0.0 3 0 0 0 8 73 1 0.0 3 0 0 0 1 74 2 0.0 3 0 0 0 2 76 1 0.0 3 0 0 0 1 77 3 0.0 3 0 0 0 3 79 2 0.0 3 0 0 0 2 83 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 89 4 0.0 3 0 0 0 4 91 1 0.0 3 0 0 0 1 92 2 0.0 3 0 0 0 2 93 2 0.0 3 0 0 1 1 94 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 98 3 0.0 3 0 0 0 3 101 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 1 110 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 117 2 0.0 3 0 1 0 1 118 2 0.0 3 0 0 0 2 119 1 0.0 3 1 120 1 0.0 3 0 0 0 1 121 3 0.0 3 0 0 0 3 122 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 162 2 0.0 3 0 0 0 2 165 1 0.0 3 0 0 0 1 168 2 0.0 3 0 0 0 2 170 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 183 3 0.0 3 0 0 0 3 186 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 191 2 0.0 3 0 0 0 2 192 5 0.0 3 0 0 0 5 197 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 281 6 0.0 3 0 0 0 6 282 3 0.0 3 0 0 0 3 284 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.95 s (36 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 26,684 Reads with adapters: 180 (0.7%) Reads written (passing filters): 26,684 (100.0%) Total basepairs processed: 7,372,757 bp Total written (filtered): 7,372,214 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 180 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 30.6% C: 38.9% G: 17.8% T: 12.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 178 416.9 0 178 4 1 104.2 0 1 5 1 26.1 0 1