This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 58.03 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 105,230 (4.7%) Reads that were too short: 40,668 (1.8%) Reads written (passing filters): 64,562 (2.9%) Total basepairs processed: 673,916,425 bp Total written (filtered): 17,090,693 bp (2.5%) === Adapter 1 === Sequence: ACAACC; Type: regular 5'; Length: 6; Trimmed: 105230 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 347 546.6 0 347 7 20730 546.6 0 20730 8 27532 546.6 0 27532 9 50 546.6 0 50 10 49 546.6 0 49 11 86 546.6 0 86 12 33 546.6 0 33 13 48 546.6 0 48 14 34 546.6 0 34 15 56 546.6 0 56 16 41 546.6 0 41 17 45 546.6 0 45 18 40 546.6 0 40 19 33 546.6 0 33 20 30 546.6 0 30 21 35 546.6 0 35 22 28 546.6 0 28 23 32 546.6 0 32 24 40 546.6 0 40 25 55 546.6 0 55 26 28 546.6 0 28 27 32 546.6 0 32 28 39 546.6 0 39 29 42 546.6 0 42 30 27 546.6 0 27 31 54 546.6 0 54 32 48 546.6 0 48 33 34 546.6 0 34 34 36 546.6 0 36 35 44 546.6 0 44 36 30 546.6 0 30 37 42 546.6 0 42 38 45 546.6 0 45 39 37 546.6 0 37 40 38 546.6 0 38 41 40 546.6 0 40 42 58 546.6 0 58 43 33 546.6 0 33 44 29 546.6 0 29 45 47 546.6 0 47 46 34 546.6 0 34 47 35 546.6 0 35 48 42 546.6 0 42 49 50 546.6 0 50 50 37 546.6 0 37 51 35 546.6 0 35 52 46 546.6 0 46 53 56 546.6 0 56 54 68 546.6 0 68 55 33 546.6 0 33 56 40 546.6 0 40 57 40 546.6 0 40 58 26 546.6 0 26 59 161 546.6 0 161 60 65 546.6 0 65 61 48 546.6 0 48 62 37 546.6 0 37 63 48 546.6 0 48 64 60 546.6 0 60 65 55 546.6 0 55 66 50 546.6 0 50 67 37 546.6 0 37 68 39 546.6 0 39 69 44 546.6 0 44 70 46 546.6 0 46 71 70 546.6 0 70 72 116 546.6 0 116 73 84 546.6 0 84 74 102 546.6 0 102 75 61 546.6 0 61 76 56 546.6 0 56 77 61 546.6 0 61 78 85 546.6 0 85 79 90 546.6 0 90 80 93 546.6 0 93 81 126 546.6 0 126 82 110 546.6 0 110 83 99 546.6 0 99 84 109 546.6 0 109 85 89 546.6 0 89 86 114 546.6 0 114 87 103 546.6 0 103 88 89 546.6 0 89 89 57 546.6 0 57 90 56 546.6 0 56 91 58 546.6 0 58 92 62 546.6 0 62 93 64 546.6 0 64 94 56 546.6 0 56 95 70 546.6 0 70 96 78 546.6 0 78 97 57 546.6 0 57 98 65 546.6 0 65 99 96 546.6 0 96 100 55 546.6 0 55 101 50 546.6 0 50 102 88 546.6 0 88 103 92 546.6 0 92 104 107 546.6 0 107 105 72 546.6 0 72 106 80 546.6 0 80 107 84 546.6 0 84 108 58 546.6 0 58 109 74 546.6 0 74 110 72 546.6 0 72 111 81 546.6 0 81 112 64 546.6 0 64 113 49 546.6 0 49 114 72 546.6 0 72 115 64 546.6 0 64 116 83 546.6 0 83 117 80 546.6 0 80 118 82 546.6 0 82 119 68 546.6 0 68 120 53 546.6 0 53 121 87 546.6 0 87 122 66 546.6 0 66 123 88 546.6 0 88 124 211 546.6 0 211 125 206 546.6 0 206 126 337 546.6 0 337 127 61 546.6 0 61 128 80 546.6 0 80 129 65 546.6 0 65 130 89 546.6 0 89 131 98 546.6 0 98 132 102 546.6 0 102 133 144 546.6 0 144 134 103 546.6 0 103 135 102 546.6 0 102 136 128 546.6 0 128 137 90 546.6 0 90 138 70 546.6 0 70 139 69 546.6 0 69 140 79 546.6 0 79 141 72 546.6 0 72 142 79 546.6 0 79 143 85 546.6 0 85 144 72 546.6 0 72 145 83 546.6 0 83 146 115 546.6 0 115 147 260 546.6 0 260 148 104 546.6 0 104 149 194 546.6 0 194 150 91 546.6 0 91 151 70 546.6 0 70 152 68 546.6 0 68 153 76 546.6 0 76 154 81 546.6 0 81 155 93 546.6 0 93 156 198 546.6 0 198 157 82 546.6 0 82 158 147 546.6 0 147 159 116 546.6 0 116 160 116 546.6 0 116 161 86 546.6 0 86 162 107 546.6 0 107 163 132 546.6 0 132 164 87 546.6 0 87 165 76 546.6 0 76 166 90 546.6 0 90 167 105 546.6 0 105 168 79 546.6 0 79 169 255 546.6 0 255 170 319 546.6 0 319 171 371 546.6 0 371 172 141 546.6 0 141 173 101 546.6 0 101 174 99 546.6 0 99 175 81 546.6 0 81 176 61 546.6 0 61 177 66 546.6 0 66 178 91 546.6 0 91 179 93 546.6 0 93 180 91 546.6 0 91 181 87 546.6 0 87 182 140 546.6 0 140 183 109 546.6 0 109 184 88 546.6 0 88 185 117 546.6 0 117 186 98 546.6 0 98 187 144 546.6 0 144 188 150 546.6 0 150 189 105 546.6 0 105 190 103 546.6 0 103 191 79 546.6 0 79 192 122 546.6 0 122 193 109 546.6 0 109 194 115 546.6 0 115 195 143 546.6 0 143 196 96 546.6 0 96 197 76 546.6 0 76 198 112 546.6 0 112 199 106 546.6 0 106 200 95 546.6 0 95 201 97 546.6 0 97 202 94 546.6 0 94 203 185 546.6 0 185 204 124 546.6 0 124 205 200 546.6 0 200 206 77 546.6 0 77 207 67 546.6 0 67 208 89 546.6 0 89 209 86 546.6 0 86 210 83 546.6 0 83 211 95 546.6 0 95 212 109 546.6 0 109 213 153 546.6 0 153 214 597 546.6 0 597 215 1509 546.6 0 1509 216 845 546.6 0 845 217 287 546.6 0 287 218 128 546.6 0 128 219 156 546.6 0 156 220 97 546.6 0 97 221 74 546.6 0 74 222 117 546.6 0 117 223 147 546.6 0 147 224 178 546.6 0 178 225 394 546.6 0 394 226 1888 546.6 0 1888 227 6915 546.6 0 6915 228 2441 546.6 0 2441 229 2676 546.6 0 2676 230 894 546.6 0 894 231 2306 546.6 0 2306 232 1041 546.6 0 1041 233 423 546.6 0 423 234 76 546.6 0 76 235 75 546.6 0 75 236 73 546.6 0 73 237 163 546.6 0 163 238 137 546.6 0 137 239 161 546.6 0 161 240 99 546.6 0 99 241 114 546.6 0 114 242 82 546.6 0 82 243 65 546.6 0 65 244 112 546.6 0 112 245 89 546.6 0 89 246 87 546.6 0 87 247 105 546.6 0 105 248 178 546.6 0 178 249 166 546.6 0 166 250 129 546.6 0 129 251 82 546.6 0 82 252 88 546.6 0 88 253 89 546.6 0 89 254 99 546.6 0 99 255 111 546.6 0 111 256 108 546.6 0 108 257 142 546.6 0 142 258 93 546.6 0 93 259 136 546.6 0 136 260 141 546.6 0 141 261 212 546.6 0 212 262 134 546.6 0 134 263 191 546.6 0 191 264 819 546.6 0 819 265 1140 546.6 0 1140 266 1586 546.6 0 1586 267 821 546.6 0 821 268 653 546.6 0 653 269 292 546.6 0 292 270 465 546.6 0 465 271 134 546.6 0 134 272 101 546.6 0 101 273 204 546.6 0 204 274 331 546.6 0 331 275 270 546.6 0 270 276 244 546.6 0 244 277 132 546.6 0 132 278 168 546.6 0 168 279 177 546.6 0 177 280 183 546.6 0 183 281 224 546.6 0 224 282 141 546.6 0 141 283 110 546.6 0 110 284 130 546.6 0 130 285 134 546.6 0 134 286 165 546.6 0 165 287 171 546.6 0 171 288 156 546.6 0 156 289 124 546.6 0 124 290 143 546.6 0 143 291 655 546.6 0 655 292 615 546.6 0 615 293 1302 546.6 0 1302 294 235 546.6 0 235 295 68 546.6 0 68 296 73 546.6 0 73 297 91 546.6 0 91 298 83 546.6 0 83 299 97 546.6 0 97 300 96 546.6 0 96 301 123 546.6 0 123 Finished in 3.18 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 64,562 Reads with adapters: 28,389 (44.0%) Reads that were too short: 14 (0.0%) Reads written (passing filters): 28,375 (44.0%) Total basepairs processed: 17,090,693 bp Total written (filtered): 7,703,848 bp (45.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 28389 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 9 0.0 3 0 0 9 19 336 0.0 3 0 329 6 0 1 20 27521 0.0 4 27069 438 14 21 65 0.0 4 11 54 22 7 0.0 4 0 0 7 23 28 0.0 4 1 1 0 24 2 28 1 0.0 4 1 46 1 0.0 4 1 60 1 0.0 4 0 0 0 0 1 61 2 0.0 4 0 0 0 0 2 72 1 0.0 4 1 118 3 0.0 4 1 0 0 2 120 1 0.0 4 0 0 0 1 126 5 0.0 4 0 0 0 0 5 127 394 0.0 4 0 0 0 0 394 128 2 0.0 4 0 0 0 0 2 129 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 144 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 158 1 0.0 4 1 208 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 242 1 0.0 4 0 0 0 0 1 250 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.98 s (35 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 28,375 Reads with adapters: 2,132 (7.5%) Reads written (passing filters): 28,375 (100.0%) Total basepairs processed: 7,703,848 bp Total written (filtered): 7,678,424 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2132 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.0% C: 3.3% G: 92.9% T: 1.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 27 443.4 0 27 5 1 27.7 0 1 8 1 0.4 0 1 9 9 0.1 0 0 9 10 145 0.0 1 4 141 11 1928 0.0 1 12 1916 12 1 0.0 1 0 1 13 1 0.0 1 0 1 16 1 0.0 1 1 19 5 0.0 1 4 1 139 2 0.0 1 2 151 1 0.0 1 0 1 185 1 0.0 1 1 186 1 0.0 1 0 1 192 1 0.0 1 0 1 209 7 0.0 1 1 6 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.