This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 58.54 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 98,427 (4.4%) Reads that were too short: 11,747 (0.5%) Reads written (passing filters): 86,680 (3.9%) Total basepairs processed: 673,916,425 bp Total written (filtered): 23,321,974 bp (3.5%) === Adapter 1 === Sequence: CTCATG; Type: regular 5'; Length: 6; Trimmed: 98427 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2385 546.6 0 2385 7 35840 546.6 0 35840 8 937 546.6 0 937 9 29484 546.6 0 29484 10 75 546.6 0 75 11 13 546.6 0 13 12 35 546.6 0 35 13 37 546.6 0 37 14 19 546.6 0 19 15 28 546.6 0 28 16 27 546.6 0 27 17 31 546.6 0 31 18 29 546.6 0 29 19 30 546.6 0 30 20 19 546.6 0 19 21 18 546.6 0 18 22 24 546.6 0 24 23 19 546.6 0 19 24 19 546.6 0 19 25 20 546.6 0 20 26 21 546.6 0 21 27 34 546.6 0 34 28 32 546.6 0 32 29 42 546.6 0 42 30 46 546.6 0 46 31 28 546.6 0 28 32 24 546.6 0 24 33 42 546.6 0 42 34 26 546.6 0 26 35 30 546.6 0 30 36 36 546.6 0 36 37 26 546.6 0 26 38 43 546.6 0 43 39 43 546.6 0 43 40 39 546.6 0 39 41 34 546.6 0 34 42 33 546.6 0 33 43 43 546.6 0 43 44 118 546.6 0 118 45 62 546.6 0 62 46 101 546.6 0 101 47 20 546.6 0 20 48 28 546.6 0 28 49 30 546.6 0 30 50 20 546.6 0 20 51 45 546.6 0 45 52 49 546.6 0 49 53 49 546.6 0 49 54 21 546.6 0 21 55 23 546.6 0 23 56 46 546.6 0 46 57 67 546.6 0 67 58 218 546.6 0 218 59 185 546.6 0 185 60 310 546.6 0 310 61 32 546.6 0 32 62 20 546.6 0 20 63 16 546.6 0 16 64 27 546.6 0 27 65 23 546.6 0 23 66 35 546.6 0 35 67 37 546.6 0 37 68 36 546.6 0 36 69 20 546.6 0 20 70 30 546.6 0 30 71 27 546.6 0 27 72 25 546.6 0 25 73 52 546.6 0 52 74 40 546.6 0 40 75 41 546.6 0 41 76 23 546.6 0 23 77 33 546.6 0 33 78 29 546.6 0 29 79 15 546.6 0 15 80 26 546.6 0 26 81 39 546.6 0 39 82 25 546.6 0 25 83 51 546.6 0 51 84 31 546.6 0 31 85 40 546.6 0 40 86 35 546.6 0 35 87 28 546.6 0 28 88 24 546.6 0 24 89 41 546.6 0 41 90 38 546.6 0 38 91 48 546.6 0 48 92 56 546.6 0 56 93 107 546.6 0 107 94 54 546.6 0 54 95 39 546.6 0 39 96 36 546.6 0 36 97 60 546.6 0 60 98 55 546.6 0 55 99 49 546.6 0 49 100 23 546.6 0 23 101 48 546.6 0 48 102 28 546.6 0 28 103 43 546.6 0 43 104 45 546.6 0 45 105 65 546.6 0 65 106 609 546.6 0 609 107 217 546.6 0 217 108 387 546.6 0 387 109 60 546.6 0 60 110 52 546.6 0 52 111 44 546.6 0 44 112 41 546.6 0 41 113 43 546.6 0 43 114 242 546.6 0 242 115 536 546.6 0 536 116 635 546.6 0 635 117 29 546.6 0 29 118 31 546.6 0 31 119 58 546.6 0 58 120 26 546.6 0 26 121 44 546.6 0 44 122 30 546.6 0 30 123 51 546.6 0 51 124 49 546.6 0 49 125 73 546.6 0 73 126 42 546.6 0 42 127 57 546.6 0 57 128 141 546.6 0 141 129 327 546.6 0 327 130 299 546.6 0 299 131 64 546.6 0 64 132 67 546.6 0 67 133 60 546.6 0 60 134 191 546.6 0 191 135 133 546.6 0 133 136 164 546.6 0 164 137 340 546.6 0 340 138 358 546.6 0 358 139 298 546.6 0 298 140 269 546.6 0 269 141 365 546.6 0 365 142 360 546.6 0 360 143 180 546.6 0 180 144 62 546.6 0 62 145 52 546.6 0 52 146 49 546.6 0 49 147 62 546.6 0 62 148 546 546.6 0 546 149 649 546.6 0 649 150 1778 546.6 0 1778 151 40 546.6 0 40 152 34 546.6 0 34 153 45 546.6 0 45 154 62 546.6 0 62 155 328 546.6 0 328 156 105 546.6 0 105 157 244 546.6 0 244 158 59 546.6 0 59 159 32 546.6 0 32 160 28 546.6 0 28 161 70 546.6 0 70 162 106 546.6 0 106 163 571 546.6 0 571 164 164 546.6 0 164 165 267 546.6 0 267 166 49 546.6 0 49 167 34 546.6 0 34 168 40 546.6 0 40 169 31 546.6 0 31 170 25 546.6 0 25 171 22 546.6 0 22 172 32 546.6 0 32 173 25 546.6 0 25 174 22 546.6 0 22 175 31 546.6 0 31 176 25 546.6 0 25 177 24 546.6 0 24 178 29 546.6 0 29 179 50 546.6 0 50 180 134 546.6 0 134 181 102 546.6 0 102 182 63 546.6 0 63 183 56 546.6 0 56 184 57 546.6 0 57 185 51 546.6 0 51 186 48 546.6 0 48 187 28 546.6 0 28 188 24 546.6 0 24 189 49 546.6 0 49 190 43 546.6 0 43 191 53 546.6 0 53 192 56 546.6 0 56 193 48 546.6 0 48 194 31 546.6 0 31 195 30 546.6 0 30 196 37 546.6 0 37 197 32 546.6 0 32 198 61 546.6 0 61 199 44 546.6 0 44 200 29 546.6 0 29 201 42 546.6 0 42 202 113 546.6 0 113 203 96 546.6 0 96 204 102 546.6 0 102 205 33 546.6 0 33 206 32 546.6 0 32 207 39 546.6 0 39 208 38 546.6 0 38 209 48 546.6 0 48 210 50 546.6 0 50 211 34 546.6 0 34 212 35 546.6 0 35 213 54 546.6 0 54 214 78 546.6 0 78 215 85 546.6 0 85 216 76 546.6 0 76 217 67 546.6 0 67 218 46 546.6 0 46 219 27 546.6 0 27 220 31 546.6 0 31 221 34 546.6 0 34 222 48 546.6 0 48 223 34 546.6 0 34 224 48 546.6 0 48 225 31 546.6 0 31 226 41 546.6 0 41 227 49 546.6 0 49 228 46 546.6 0 46 229 43 546.6 0 43 230 38 546.6 0 38 231 56 546.6 0 56 232 76 546.6 0 76 233 110 546.6 0 110 234 146 546.6 0 146 235 105 546.6 0 105 236 58 546.6 0 58 237 36 546.6 0 36 238 42 546.6 0 42 239 33 546.6 0 33 240 51 546.6 0 51 241 38 546.6 0 38 242 57 546.6 0 57 243 61 546.6 0 61 244 64 546.6 0 64 245 48 546.6 0 48 246 34 546.6 0 34 247 58 546.6 0 58 248 84 546.6 0 84 249 40 546.6 0 40 250 34 546.6 0 34 251 68 546.6 0 68 252 69 546.6 0 69 253 94 546.6 0 94 254 81 546.6 0 81 255 134 546.6 0 134 256 60 546.6 0 60 257 79 546.6 0 79 258 56 546.6 0 56 259 50 546.6 0 50 260 70 546.6 0 70 261 92 546.6 0 92 262 112 546.6 0 112 263 233 546.6 0 233 264 66 546.6 0 66 265 59 546.6 0 59 266 79 546.6 0 79 267 96 546.6 0 96 268 45 546.6 0 45 269 107 546.6 0 107 270 124 546.6 0 124 271 632 546.6 0 632 272 300 546.6 0 300 273 469 546.6 0 469 274 65 546.6 0 65 275 58 546.6 0 58 276 59 546.6 0 59 277 56 546.6 0 56 278 118 546.6 0 118 279 169 546.6 0 169 280 144 546.6 0 144 281 64 546.6 0 64 282 61 546.6 0 61 283 93 546.6 0 93 284 100 546.6 0 100 285 85 546.6 0 85 286 55 546.6 0 55 287 58 546.6 0 58 288 59 546.6 0 59 289 68 546.6 0 68 290 104 546.6 0 104 291 100 546.6 0 100 292 191 546.6 0 191 293 151 546.6 0 151 294 401 546.6 0 401 295 1358 546.6 0 1358 296 1069 546.6 0 1069 297 874 546.6 0 874 298 149 546.6 0 149 299 44 546.6 0 44 300 59 546.6 0 59 301 33 546.6 0 33 Finished in 3.95 s (46 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 86,680 Reads with adapters: 37,883 (43.7%) Reads that were too short: 96 (0.1%) Reads written (passing filters): 37,787 (43.6%) Total basepairs processed: 23,321,974 bp Total written (filtered): 10,430,791 bp (44.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 37883 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 17 0.0 3 0 0 8 9 16 309 0.0 3 0 258 36 15 17 35368 0.0 3 33257 1759 284 68 18 118 0.0 3 9 78 6 25 19 106 0.0 3 5 14 53 34 20 1645 0.0 3 1407 66 12 160 21 4 0.0 3 0 2 0 2 22 2 0.0 3 0 0 0 2 23 5 0.0 3 0 0 0 5 27 1 0.0 3 0 0 0 1 30 9 0.0 3 0 0 0 9 31 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 43 65 0.0 3 0 0 0 65 45 1 0.0 3 0 0 0 1 46 13 0.0 3 0 0 1 12 48 2 0.0 3 0 0 0 2 49 3 0.0 3 0 0 0 3 50 1 0.0 3 0 0 0 1 51 2 0.0 3 0 0 0 2 54 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 63 2 0.0 3 0 0 0 2 64 6 0.0 3 0 0 0 6 65 2 0.0 3 0 0 0 2 66 2 0.0 3 1 0 0 1 68 2 0.0 3 0 0 0 2 69 30 0.0 3 0 0 0 30 70 24 0.0 3 0 0 0 24 71 1 0.0 3 0 0 0 1 72 4 0.0 3 0 0 0 4 73 5 0.0 3 0 0 1 4 77 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 113 12 0.0 3 0 0 0 12 114 2 0.0 3 0 0 0 2 115 1 0.0 3 0 0 0 1 116 2 0.0 3 0 0 0 2 123 2 0.0 3 0 0 0 2 124 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 132 3 0.0 3 0 1 1 1 133 1 0.0 3 0 0 0 1 134 3 0.0 3 0 0 0 3 136 1 0.0 3 0 0 0 1 139 4 0.0 3 0 0 0 4 142 1 0.0 3 0 0 0 1 144 3 0.0 3 0 0 0 3 145 2 0.0 3 0 0 0 2 151 1 0.0 3 1 152 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 156 3 0.0 3 0 0 0 3 161 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 164 2 0.0 3 0 0 0 2 165 4 0.0 3 0 0 0 4 168 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 1 184 2 0.0 3 0 0 0 2 187 1 0.0 3 0 0 0 1 189 2 0.0 3 0 0 0 2 191 1 0.0 3 0 0 0 1 192 3 0.0 3 0 0 0 3 193 1 0.0 3 0 0 0 1 194 2 0.0 3 0 0 0 2 195 1 0.0 3 0 0 1 200 1 0.0 3 0 0 0 1 205 4 0.0 3 0 0 0 4 209 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 217 5 0.0 3 0 0 1 4 225 2 0.0 3 0 0 0 2 238 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 253 1 0.0 3 0 0 0 1 257 1 0.0 3 0 0 0 1 261 1 0.0 3 0 0 0 1 275 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 7 0.0 3 0 0 0 7 280 2 0.0 3 0 0 0 2 281 6 0.0 3 0 0 0 6 282 5 0.0 3 0 0 0 5 283 2 0.0 3 0 0 0 2 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.33 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 37,787 Reads with adapters: 1,090 (2.9%) Reads written (passing filters): 37,787 (100.0%) Total basepairs processed: 10,430,791 bp Total written (filtered): 10,427,501 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1090 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 52.4% C: 28.3% G: 11.1% T: 8.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1076 590.4 0 1076 4 10 147.6 0 10 5 2 36.9 0 2 6 2 9.2 0 2