This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 57.91 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 89,485 (4.0%) Reads that were too short: 5,464 (0.2%) Reads written (passing filters): 84,021 (3.8%) Total basepairs processed: 673,916,425 bp Total written (filtered): 22,988,448 bp (3.4%) === Adapter 1 === Sequence: CTCATG; Type: regular 5'; Length: 6; Trimmed: 89485 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1783 546.6 0 1783 7 31508 546.6 0 31508 8 946 546.6 0 946 9 36048 546.6 0 36048 10 46 546.6 0 46 11 15 546.6 0 15 12 24 546.6 0 24 13 12 546.6 0 12 14 8 546.6 0 8 15 15 546.6 0 15 16 18 546.6 0 18 17 20 546.6 0 20 18 22 546.6 0 22 19 13 546.6 0 13 20 10 546.6 0 10 21 15 546.6 0 15 22 18 546.6 0 18 23 10 546.6 0 10 24 18 546.6 0 18 25 8 546.6 0 8 26 12 546.6 0 12 27 18 546.6 0 18 28 18 546.6 0 18 29 13 546.6 0 13 30 20 546.6 0 20 31 26 546.6 0 26 32 17 546.6 0 17 33 26 546.6 0 26 34 14 546.6 0 14 35 14 546.6 0 14 36 24 546.6 0 24 37 10 546.6 0 10 38 25 546.6 0 25 39 14 546.6 0 14 40 27 546.6 0 27 41 16 546.6 0 16 42 17 546.6 0 17 43 34 546.6 0 34 44 131 546.6 0 131 45 53 546.6 0 53 46 64 546.6 0 64 47 17 546.6 0 17 48 23 546.6 0 23 49 19 546.6 0 19 50 17 546.6 0 17 51 21 546.6 0 21 52 24 546.6 0 24 53 16 546.6 0 16 54 27 546.6 0 27 55 16 546.6 0 16 56 27 546.6 0 27 57 20 546.6 0 20 58 50 546.6 0 50 59 43 546.6 0 43 60 33 546.6 0 33 61 19 546.6 0 19 62 12 546.6 0 12 63 19 546.6 0 19 64 19 546.6 0 19 65 28 546.6 0 28 66 17 546.6 0 17 67 17 546.6 0 17 68 16 546.6 0 16 69 21 546.6 0 21 70 14 546.6 0 14 71 13 546.6 0 13 72 18 546.6 0 18 73 24 546.6 0 24 74 19 546.6 0 19 75 22 546.6 0 22 76 21 546.6 0 21 77 20 546.6 0 20 78 14 546.6 0 14 79 8 546.6 0 8 80 28 546.6 0 28 81 23 546.6 0 23 82 22 546.6 0 22 83 31 546.6 0 31 84 19 546.6 0 19 85 21 546.6 0 21 86 26 546.6 0 26 87 19 546.6 0 19 88 24 546.6 0 24 89 32 546.6 0 32 90 27 546.6 0 27 91 39 546.6 0 39 92 35 546.6 0 35 93 47 546.6 0 47 94 39 546.6 0 39 95 27 546.6 0 27 96 26 546.6 0 26 97 35 546.6 0 35 98 36 546.6 0 36 99 24 546.6 0 24 100 26 546.6 0 26 101 26 546.6 0 26 102 29 546.6 0 29 103 32 546.6 0 32 104 40 546.6 0 40 105 64 546.6 0 64 106 612 546.6 0 612 107 188 546.6 0 188 108 385 546.6 0 385 109 48 546.6 0 48 110 25 546.6 0 25 111 41 546.6 0 41 112 33 546.6 0 33 113 37 546.6 0 37 114 252 546.6 0 252 115 601 546.6 0 601 116 753 546.6 0 753 117 21 546.6 0 21 118 15 546.6 0 15 119 29 546.6 0 29 120 32 546.6 0 32 121 39 546.6 0 39 122 28 546.6 0 28 123 28 546.6 0 28 124 25 546.6 0 25 125 28 546.6 0 28 126 25 546.6 0 25 127 46 546.6 0 46 128 68 546.6 0 68 129 134 546.6 0 134 130 115 546.6 0 115 131 35 546.6 0 35 132 61 546.6 0 61 133 49 546.6 0 49 134 162 546.6 0 162 135 139 546.6 0 139 136 133 546.6 0 133 137 316 546.6 0 316 138 363 546.6 0 363 139 216 546.6 0 216 140 229 546.6 0 229 141 278 546.6 0 278 142 221 546.6 0 221 143 99 546.6 0 99 144 41 546.6 0 41 145 38 546.6 0 38 146 43 546.6 0 43 147 37 546.6 0 37 148 589 546.6 0 589 149 612 546.6 0 612 150 1940 546.6 0 1940 151 36 546.6 0 36 152 18 546.6 0 18 153 31 546.6 0 31 154 25 546.6 0 25 155 149 546.6 0 149 156 52 546.6 0 52 157 175 546.6 0 175 158 55 546.6 0 55 159 16 546.6 0 16 160 22 546.6 0 22 161 35 546.6 0 35 162 67 546.6 0 67 163 261 546.6 0 261 164 82 546.6 0 82 165 163 546.6 0 163 166 26 546.6 0 26 167 16 546.6 0 16 168 20 546.6 0 20 169 23 546.6 0 23 170 20 546.6 0 20 171 11 546.6 0 11 172 11 546.6 0 11 173 20 546.6 0 20 174 20 546.6 0 20 175 21 546.6 0 21 176 23 546.6 0 23 177 27 546.6 0 27 178 22 546.6 0 22 179 29 546.6 0 29 180 64 546.6 0 64 181 80 546.6 0 80 182 39 546.6 0 39 183 31 546.6 0 31 184 27 546.6 0 27 185 25 546.6 0 25 186 27 546.6 0 27 187 17 546.6 0 17 188 17 546.6 0 17 189 45 546.6 0 45 190 23 546.6 0 23 191 24 546.6 0 24 192 33 546.6 0 33 193 31 546.6 0 31 194 14 546.6 0 14 195 15 546.6 0 15 196 19 546.6 0 19 197 23 546.6 0 23 198 63 546.6 0 63 199 26 546.6 0 26 200 23 546.6 0 23 201 29 546.6 0 29 202 70 546.6 0 70 203 71 546.6 0 71 204 60 546.6 0 60 205 23 546.6 0 23 206 9 546.6 0 9 207 18 546.6 0 18 208 21 546.6 0 21 209 49 546.6 0 49 210 25 546.6 0 25 211 21 546.6 0 21 212 20 546.6 0 20 213 23 546.6 0 23 214 43 546.6 0 43 215 37 546.6 0 37 216 37 546.6 0 37 217 33 546.6 0 33 218 32 546.6 0 32 219 20 546.6 0 20 220 27 546.6 0 27 221 32 546.6 0 32 222 32 546.6 0 32 223 27 546.6 0 27 224 39 546.6 0 39 225 25 546.6 0 25 226 17 546.6 0 17 227 18 546.6 0 18 228 26 546.6 0 26 229 28 546.6 0 28 230 20 546.6 0 20 231 28 546.6 0 28 232 37 546.6 0 37 233 39 546.6 0 39 234 57 546.6 0 57 235 58 546.6 0 58 236 31 546.6 0 31 237 40 546.6 0 40 238 39 546.6 0 39 239 25 546.6 0 25 240 39 546.6 0 39 241 29 546.6 0 29 242 29 546.6 0 29 243 29 546.6 0 29 244 43 546.6 0 43 245 32 546.6 0 32 246 34 546.6 0 34 247 34 546.6 0 34 248 98 546.6 0 98 249 40 546.6 0 40 250 32 546.6 0 32 251 39 546.6 0 39 252 35 546.6 0 35 253 64 546.6 0 64 254 49 546.6 0 49 255 48 546.6 0 48 256 36 546.6 0 36 257 32 546.6 0 32 258 28 546.6 0 28 259 33 546.6 0 33 260 41 546.6 0 41 261 41 546.6 0 41 262 54 546.6 0 54 263 101 546.6 0 101 264 39 546.6 0 39 265 27 546.6 0 27 266 33 546.6 0 33 267 59 546.6 0 59 268 39 546.6 0 39 269 48 546.6 0 48 270 56 546.6 0 56 271 174 546.6 0 174 272 119 546.6 0 119 273 182 546.6 0 182 274 51 546.6 0 51 275 74 546.6 0 74 276 53 546.6 0 53 277 43 546.6 0 43 278 59 546.6 0 59 279 71 546.6 0 71 280 68 546.6 0 68 281 38 546.6 0 38 282 32 546.6 0 32 283 55 546.6 0 55 284 39 546.6 0 39 285 57 546.6 0 57 286 29 546.6 0 29 287 37 546.6 0 37 288 43 546.6 0 43 289 46 546.6 0 46 290 57 546.6 0 57 291 58 546.6 0 58 292 89 546.6 0 89 293 71 546.6 0 71 294 138 546.6 0 138 295 415 546.6 0 415 296 340 546.6 0 340 297 249 546.6 0 249 298 62 546.6 0 62 299 42 546.6 0 42 300 45 546.6 0 45 301 30 546.6 0 30 Finished in 4.24 s (50 us/read; 1.19 M reads/minute). === Summary === Total reads processed: 84,021 Reads with adapters: 37,966 (45.2%) Reads that were too short: 3 (0.0%) Reads written (passing filters): 37,963 (45.2%) Total basepairs processed: 22,988,448 bp Total written (filtered): 10,275,276 bp (44.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 37966 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 0 2 18 15 0.0 3 0 0 13 2 19 379 0.0 3 0 374 3 1 1 20 36939 0.0 4 36257 641 34 6 1 21 93 0.0 4 12 77 4 22 6 0.0 4 0 0 6 23 26 0.0 4 0 0 0 24 2 24 1 0.0 4 1 41 1 0.0 4 1 68 1 0.0 4 0 0 0 0 1 80 1 0.0 4 1 91 1 0.0 4 1 125 2 0.0 4 0 0 0 0 2 126 2 0.0 4 0 0 0 0 2 127 490 0.0 4 0 0 0 0 490 128 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 203 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.35 s (36 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 37,963 Reads with adapters: 7,548 (19.9%) Reads written (passing filters): 37,963 (100.0%) Total basepairs processed: 10,275,276 bp Total written (filtered): 10,165,418 bp (98.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 7548 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.8% C: 2.2% G: 94.0% T: 1.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 27 593.2 0 27 4 6 148.3 0 6 5 1 37.1 0 1 6 6 9.3 0 6 9 4730 0.1 0 124 4606 10 2414 0.0 1 87 2327 11 187 0.0 1 2 185 12 11 0.0 1 0 11 13 3 0.0 1 1 2 101 1 0.0 1 1 150 3 0.0 1 0 3 168 1 0.0 1 1 208 7 0.0 1 5 2 210 2 0.0 1 0 2 211 1 0.0 1 0 1 215 1 0.0 1 1 223 1 0.0 1 1 257 141 0.0 1 135 6 258 3 0.0 1 3 259 1 0.0 1 1 260 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.