This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGACGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 58.79 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 320,485 (14.3%) Reads that were too short: 31,861 (1.4%) Reads written (passing filters): 288,624 (12.9%) Total basepairs processed: 673,916,425 bp Total written (filtered): 82,821,181 bp (12.3%) === Adapter 1 === Sequence: CGACGT; Type: regular 5'; Length: 6; Trimmed: 320485 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 6698 546.6 0 6698 7 116726 546.6 0 116726 8 7132 546.6 0 7132 9 145011 546.6 0 145011 10 118 546.6 0 118 11 33 546.6 0 33 12 26 546.6 0 26 13 18 546.6 0 18 14 29 546.6 0 29 15 16 546.6 0 16 16 124 546.6 0 124 17 65 546.6 0 65 18 65 546.6 0 65 19 13 546.6 0 13 20 16 546.6 0 16 21 17 546.6 0 17 22 13 546.6 0 13 23 4 546.6 0 4 24 25 546.6 0 25 25 27 546.6 0 27 26 17 546.6 0 17 27 16 546.6 0 16 28 18 546.6 0 18 29 10 546.6 0 10 30 18 546.6 0 18 31 21 546.6 0 21 32 17 546.6 0 17 33 17 546.6 0 17 34 21 546.6 0 21 35 23 546.6 0 23 36 18 546.6 0 18 37 17 546.6 0 17 38 20 546.6 0 20 39 15 546.6 0 15 40 17 546.6 0 17 41 10 546.6 0 10 42 9 546.6 0 9 43 16 546.6 0 16 44 21 546.6 0 21 45 14 546.6 0 14 46 13 546.6 0 13 47 22 546.6 0 22 48 29 546.6 0 29 49 23 546.6 0 23 50 96 546.6 0 96 51 85 546.6 0 85 52 12 546.6 0 12 53 27 546.6 0 27 54 20 546.6 0 20 55 21 546.6 0 21 56 21 546.6 0 21 57 26 546.6 0 26 58 10 546.6 0 10 59 37 546.6 0 37 60 27 546.6 0 27 61 97 546.6 0 97 62 25 546.6 0 25 63 24 546.6 0 24 64 19 546.6 0 19 65 20 546.6 0 20 66 24 546.6 0 24 67 281 546.6 0 281 68 160 546.6 0 160 69 323 546.6 0 323 70 13 546.6 0 13 71 12 546.6 0 12 72 40 546.6 0 40 73 38 546.6 0 38 74 33 546.6 0 33 75 23 546.6 0 23 76 18 546.6 0 18 77 21 546.6 0 21 78 16 546.6 0 16 79 20 546.6 0 20 80 22 546.6 0 22 81 28 546.6 0 28 82 12 546.6 0 12 83 19 546.6 0 19 84 21 546.6 0 21 85 56 546.6 0 56 86 107 546.6 0 107 87 53 546.6 0 53 88 21 546.6 0 21 89 21 546.6 0 21 90 40 546.6 0 40 91 18 546.6 0 18 92 67 546.6 0 67 93 25 546.6 0 25 94 92 546.6 0 92 95 23 546.6 0 23 96 27 546.6 0 27 97 20 546.6 0 20 98 18 546.6 0 18 99 10 546.6 0 10 100 25 546.6 0 25 101 9 546.6 0 9 102 31 546.6 0 31 103 10 546.6 0 10 104 17 546.6 0 17 105 14 546.6 0 14 106 11 546.6 0 11 107 25 546.6 0 25 108 26 546.6 0 26 109 17 546.6 0 17 110 12 546.6 0 12 111 25 546.6 0 25 112 17 546.6 0 17 113 23 546.6 0 23 114 17 546.6 0 17 115 15 546.6 0 15 116 24 546.6 0 24 117 14 546.6 0 14 118 49 546.6 0 49 119 56 546.6 0 56 120 77 546.6 0 77 121 40 546.6 0 40 122 36 546.6 0 36 123 44 546.6 0 44 124 20 546.6 0 20 125 24 546.6 0 24 126 37 546.6 0 37 127 44 546.6 0 44 128 50 546.6 0 50 129 75 546.6 0 75 130 105 546.6 0 105 131 128 546.6 0 128 132 90 546.6 0 90 133 118 546.6 0 118 134 114 546.6 0 114 135 26 546.6 0 26 136 32 546.6 0 32 137 12 546.6 0 12 138 24 546.6 0 24 139 21 546.6 0 21 140 34 546.6 0 34 141 26 546.6 0 26 142 21 546.6 0 21 143 23 546.6 0 23 144 23 546.6 0 23 145 44 546.6 0 44 146 39 546.6 0 39 147 67 546.6 0 67 148 47 546.6 0 47 149 55 546.6 0 55 150 46 546.6 0 46 151 36 546.6 0 36 152 148 546.6 0 148 153 279 546.6 0 279 154 326 546.6 0 326 155 118 546.6 0 118 156 25 546.6 0 25 157 34 546.6 0 34 158 147 546.6 0 147 159 88 546.6 0 88 160 139 546.6 0 139 161 52 546.6 0 52 162 42 546.6 0 42 163 139 546.6 0 139 164 160 546.6 0 160 165 340 546.6 0 340 166 157 546.6 0 157 167 110 546.6 0 110 168 60 546.6 0 60 169 57 546.6 0 57 170 28 546.6 0 28 171 26 546.6 0 26 172 45 546.6 0 45 173 65 546.6 0 65 174 78 546.6 0 78 175 90 546.6 0 90 176 67 546.6 0 67 177 86 546.6 0 86 178 88 546.6 0 88 179 94 546.6 0 94 180 148 546.6 0 148 181 108 546.6 0 108 182 94 546.6 0 94 183 358 546.6 0 358 184 338 546.6 0 338 185 727 546.6 0 727 186 408 546.6 0 408 187 94 546.6 0 94 188 54 546.6 0 54 189 50 546.6 0 50 190 49 546.6 0 49 191 61 546.6 0 61 192 245 546.6 0 245 193 338 546.6 0 338 194 443 546.6 0 443 195 178 546.6 0 178 196 51 546.6 0 51 197 39 546.6 0 39 198 158 546.6 0 158 199 200 546.6 0 200 200 342 546.6 0 342 201 151 546.6 0 151 202 144 546.6 0 144 203 94 546.6 0 94 204 119 546.6 0 119 205 91 546.6 0 91 206 113 546.6 0 113 207 147 546.6 0 147 208 106 546.6 0 106 209 91 546.6 0 91 210 84 546.6 0 84 211 78 546.6 0 78 212 66 546.6 0 66 213 65 546.6 0 65 214 100 546.6 0 100 215 132 546.6 0 132 216 170 546.6 0 170 217 374 546.6 0 374 218 718 546.6 0 718 219 8546 546.6 0 8546 220 2477 546.6 0 2477 221 6808 546.6 0 6808 222 174 546.6 0 174 223 71 546.6 0 71 224 85 546.6 0 85 225 46 546.6 0 46 226 36 546.6 0 36 227 31 546.6 0 31 228 38 546.6 0 38 229 47 546.6 0 47 230 78 546.6 0 78 231 59 546.6 0 59 232 61 546.6 0 61 233 61 546.6 0 61 234 75 546.6 0 75 235 95 546.6 0 95 236 69 546.6 0 69 237 93 546.6 0 93 238 105 546.6 0 105 239 100 546.6 0 100 240 137 546.6 0 137 241 67 546.6 0 67 242 51 546.6 0 51 243 43 546.6 0 43 244 54 546.6 0 54 245 46 546.6 0 46 246 203 546.6 0 203 247 79 546.6 0 79 248 245 546.6 0 245 249 323 546.6 0 323 250 616 546.6 0 616 251 704 546.6 0 704 252 193 546.6 0 193 253 210 546.6 0 210 254 210 546.6 0 210 255 98 546.6 0 98 256 103 546.6 0 103 257 81 546.6 0 81 258 76 546.6 0 76 259 44 546.6 0 44 260 60 546.6 0 60 261 48 546.6 0 48 262 62 546.6 0 62 263 71 546.6 0 71 264 44 546.6 0 44 265 55 546.6 0 55 266 73 546.6 0 73 267 153 546.6 0 153 268 105 546.6 0 105 269 78 546.6 0 78 270 71 546.6 0 71 271 72 546.6 0 72 272 155 546.6 0 155 273 152 546.6 0 152 274 245 546.6 0 245 275 92 546.6 0 92 276 49 546.6 0 49 277 86 546.6 0 86 278 184 546.6 0 184 279 709 546.6 0 709 280 830 546.6 0 830 281 584 546.6 0 584 282 188 546.6 0 188 283 108 546.6 0 108 284 128 546.6 0 128 285 82 546.6 0 82 286 89 546.6 0 89 287 102 546.6 0 102 288 139 546.6 0 139 289 106 546.6 0 106 290 79 546.6 0 79 291 100 546.6 0 100 292 172 546.6 0 172 293 167 546.6 0 167 294 191 546.6 0 191 295 120 546.6 0 120 296 104 546.6 0 104 297 60 546.6 0 60 298 87 546.6 0 87 299 119 546.6 0 119 300 152 546.6 0 152 301 160 546.6 0 160 Finished in 12.54 s (43 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 288,624 Reads with adapters: 153,554 (53.2%) Reads that were too short: 196 (0.1%) Reads written (passing filters): 153,358 (53.1%) Total basepairs processed: 82,821,181 bp Total written (filtered): 42,164,372 bp (50.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 153554 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 21 0.0 2 0 0 0 21 15 104 0.0 3 0 0 73 31 16 1184 0.0 3 0 999 126 59 17 150629 0.0 3 141212 7728 1381 308 18 569 0.0 3 26 368 39 136 19 337 0.0 3 2 0 178 157 20 394 0.0 3 1 1 1 391 23 3 0.0 3 1 0 1 1 25 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 34 4 0.0 3 0 0 0 4 37 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 39 5 0.0 3 0 0 0 5 42 2 0.0 3 0 0 0 2 44 7 0.0 3 0 0 0 7 48 2 0.0 3 0 0 0 2 53 4 0.0 3 0 0 0 4 54 13 0.0 3 0 0 0 13 55 2 0.0 3 0 0 0 2 56 1 0.0 3 0 0 0 1 57 2 0.0 3 0 0 0 2 59 4 0.0 3 0 0 0 4 60 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 1 63 2 0.0 3 0 0 0 2 66 1 0.0 3 0 0 0 1 68 2 0.0 3 0 0 0 2 76 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 1 92 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 95 2 0.0 3 0 0 0 2 96 1 0.0 3 0 0 0 1 99 7 0.0 3 0 0 0 7 100 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 104 7 0.0 3 0 1 0 6 105 2 0.0 3 0 0 0 2 106 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 108 2 0.0 3 0 0 0 2 109 25 0.0 3 0 0 0 25 110 1 0.0 3 0 0 0 1 112 3 0.0 3 0 0 0 3 114 2 0.0 3 0 0 0 2 115 2 0.0 3 0 0 0 2 116 3 0.0 3 0 0 0 3 117 1 0.0 3 0 0 0 1 118 1 0.0 3 1 120 4 0.0 3 0 0 0 4 123 6 0.0 3 0 0 0 6 124 2 0.0 3 0 0 0 2 129 1 0.0 3 0 0 0 1 130 2 0.0 3 0 0 0 2 134 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 141 2 0.0 3 0 0 0 2 146 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 151 2 0.0 3 0 0 0 2 154 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 165 3 0.0 3 0 0 0 3 166 1 0.0 3 0 0 0 1 167 2 0.0 3 0 0 0 2 168 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 183 5 0.0 3 0 0 0 5 184 1 0.0 3 0 0 0 1 185 2 0.0 3 0 0 1 1 186 1 0.0 3 0 0 1 187 1 0.0 3 0 0 0 1 192 12 0.0 3 0 0 0 12 194 7 0.0 3 0 0 0 7 195 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 205 3 0.0 3 0 0 0 3 206 3 0.0 3 0 0 1 2 213 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 20 0.0 3 0 0 2 18 219 1 0.0 3 0 0 0 1 221 3 0.0 3 0 0 0 3 222 3 0.0 3 0 0 0 3 226 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 240 2 0.0 3 0 0 0 2 243 1 0.0 3 0 0 0 1 246 1 0.0 3 0 0 0 1 261 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 0 1 277 1 0.0 3 0 0 1 278 1 0.0 3 0 0 0 1 279 7 0.0 3 0 0 0 7 280 10 0.0 3 0 0 0 10 281 17 0.0 3 0 0 0 17 282 19 0.0 3 0 0 3 16 283 9 0.0 3 0 0 0 9 287 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 289 1 0.0 3 0 0 0 1 294 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 5.31 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 153,358 Reads with adapters: 4,836 (3.2%) Reads written (passing filters): 153,358 (100.0%) Total basepairs processed: 42,164,372 bp Total written (filtered): 42,149,709 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 4836 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 63.3% C: 15.9% G: 17.8% T: 2.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 4686 2396.2 0 4686 4 145 599.1 0 145 5 5 149.8 0 5