This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTCGGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 71.83 s (27 us/read; 2.22 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 482,851 (18.2%) Reads that were too short: 30,247 (1.1%) Reads written (passing filters): 452,604 (17.0%) Total basepairs processed: 800,111,879 bp Total written (filtered): 104,199,123 bp (13.0%) === Adapter 1 === Sequence: TTCGGA; Type: regular 5'; Length: 6; Trimmed: 482851 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 840 649.0 0 840 7 2103 649.0 0 2103 8 37165 649.0 0 37165 9 37951 649.0 0 37951 10 52898 649.0 0 52898 11 67 649.0 0 67 12 28 649.0 0 28 13 17 649.0 0 17 14 11 649.0 0 11 15 27 649.0 0 27 16 34 649.0 0 34 17 40 649.0 0 40 18 19 649.0 0 19 19 32 649.0 0 32 20 21 649.0 0 21 21 23 649.0 0 23 22 29 649.0 0 29 23 61 649.0 0 61 24 71 649.0 0 71 25 41 649.0 0 41 26 71 649.0 0 71 27 57 649.0 0 57 28 30 649.0 0 30 29 30 649.0 0 30 30 33 649.0 0 33 31 46 649.0 0 46 32 60 649.0 0 60 33 27 649.0 0 27 34 20 649.0 0 20 35 23 649.0 0 23 36 24 649.0 0 24 37 12 649.0 0 12 38 37 649.0 0 37 39 86 649.0 0 86 40 104 649.0 0 104 41 170 649.0 0 170 42 58 649.0 0 58 43 23 649.0 0 23 44 28 649.0 0 28 45 22 649.0 0 22 46 17 649.0 0 17 47 34 649.0 0 34 48 63 649.0 0 63 49 62 649.0 0 62 50 49 649.0 0 49 51 87 649.0 0 87 52 162 649.0 0 162 53 154 649.0 0 154 54 112 649.0 0 112 55 40 649.0 0 40 56 38 649.0 0 38 57 94 649.0 0 94 58 637 649.0 0 637 59 52 649.0 0 52 60 63 649.0 0 63 61 39 649.0 0 39 62 60 649.0 0 60 63 76 649.0 0 76 64 42 649.0 0 42 65 52 649.0 0 52 66 93 649.0 0 93 67 67 649.0 0 67 68 153 649.0 0 153 69 64 649.0 0 64 70 73 649.0 0 73 71 110 649.0 0 110 72 167 649.0 0 167 73 96 649.0 0 96 74 121 649.0 0 121 75 208 649.0 0 208 76 290 649.0 0 290 77 356 649.0 0 356 78 759 649.0 0 759 79 1635 649.0 0 1635 80 432 649.0 0 432 81 588 649.0 0 588 82 1235 649.0 0 1235 83 582 649.0 0 582 84 570 649.0 0 570 85 1084 649.0 0 1084 86 1503 649.0 0 1503 87 1762 649.0 0 1762 88 1189 649.0 0 1189 89 2236 649.0 0 2236 90 2988 649.0 0 2988 91 5368 649.0 0 5368 92 99703 649.0 0 99703 93 52236 649.0 0 52236 94 127710 649.0 0 127710 95 910 649.0 0 910 96 52 649.0 0 52 97 53 649.0 0 53 98 50 649.0 0 50 99 37 649.0 0 37 100 24 649.0 0 24 101 13 649.0 0 13 102 26 649.0 0 26 103 19 649.0 0 19 104 19 649.0 0 19 105 22 649.0 0 22 106 18 649.0 0 18 107 22 649.0 0 22 108 32 649.0 0 32 109 23 649.0 0 23 110 48 649.0 0 48 111 36 649.0 0 36 112 91 649.0 0 91 113 29 649.0 0 29 114 59 649.0 0 59 115 57 649.0 0 57 116 66 649.0 0 66 117 98 649.0 0 98 118 35 649.0 0 35 119 17 649.0 0 17 120 14 649.0 0 14 121 10 649.0 0 10 122 9 649.0 0 9 123 17 649.0 0 17 124 12 649.0 0 12 125 25 649.0 0 25 126 21 649.0 0 21 127 50 649.0 0 50 128 33 649.0 0 33 129 57 649.0 0 57 130 47 649.0 0 47 131 44 649.0 0 44 132 27 649.0 0 27 133 29 649.0 0 29 134 29 649.0 0 29 135 22 649.0 0 22 136 25 649.0 0 25 137 38 649.0 0 38 138 30 649.0 0 30 139 31 649.0 0 31 140 19 649.0 0 19 141 18 649.0 0 18 142 29 649.0 0 29 143 48 649.0 0 48 144 42 649.0 0 42 145 66 649.0 0 66 146 17 649.0 0 17 147 43 649.0 0 43 148 26 649.0 0 26 149 19 649.0 0 19 150 14 649.0 0 14 151 29 649.0 0 29 152 12 649.0 0 12 153 17 649.0 0 17 154 22 649.0 0 22 155 17 649.0 0 17 156 25 649.0 0 25 157 33 649.0 0 33 158 73 649.0 0 73 159 28 649.0 0 28 160 30 649.0 0 30 161 24 649.0 0 24 162 15 649.0 0 15 163 19 649.0 0 19 164 23 649.0 0 23 165 26 649.0 0 26 166 32 649.0 0 32 167 15 649.0 0 15 168 19 649.0 0 19 169 17 649.0 0 17 170 21 649.0 0 21 171 26 649.0 0 26 172 36 649.0 0 36 173 31 649.0 0 31 174 46 649.0 0 46 175 39 649.0 0 39 176 71 649.0 0 71 177 228 649.0 0 228 178 1035 649.0 0 1035 179 1567 649.0 0 1567 180 591 649.0 0 591 181 33 649.0 0 33 182 23 649.0 0 23 183 36 649.0 0 36 184 35 649.0 0 35 185 35 649.0 0 35 186 52 649.0 0 52 187 72 649.0 0 72 188 165 649.0 0 165 189 68 649.0 0 68 190 117 649.0 0 117 191 133 649.0 0 133 192 90 649.0 0 90 193 46 649.0 0 46 194 96 649.0 0 96 195 119 649.0 0 119 196 150 649.0 0 150 197 161 649.0 0 161 198 183 649.0 0 183 199 234 649.0 0 234 200 399 649.0 0 399 201 5673 649.0 0 5673 202 4173 649.0 0 4173 203 7890 649.0 0 7890 204 223 649.0 0 223 205 495 649.0 0 495 206 777 649.0 0 777 207 891 649.0 0 891 208 303 649.0 0 303 209 226 649.0 0 226 210 134 649.0 0 134 211 61 649.0 0 61 212 72 649.0 0 72 213 55 649.0 0 55 214 51 649.0 0 51 215 107 649.0 0 107 216 106 649.0 0 106 217 107 649.0 0 107 218 79 649.0 0 79 219 43 649.0 0 43 220 52 649.0 0 52 221 207 649.0 0 207 222 361 649.0 0 361 223 551 649.0 0 551 224 253 649.0 0 253 225 485 649.0 0 485 226 689 649.0 0 689 227 867 649.0 0 867 228 657 649.0 0 657 229 513 649.0 0 513 230 19 649.0 0 19 231 28 649.0 0 28 232 33 649.0 0 33 233 27 649.0 0 27 234 17 649.0 0 17 235 30 649.0 0 30 236 82 649.0 0 82 237 69 649.0 0 69 238 70 649.0 0 70 239 23 649.0 0 23 240 15 649.0 0 15 241 16 649.0 0 16 242 17 649.0 0 17 243 37 649.0 0 37 244 36 649.0 0 36 245 36 649.0 0 36 246 84 649.0 0 84 247 54 649.0 0 54 248 24 649.0 0 24 249 32 649.0 0 32 250 26 649.0 0 26 251 32 649.0 0 32 252 17 649.0 0 17 253 28 649.0 0 28 254 24 649.0 0 24 255 29 649.0 0 29 256 33 649.0 0 33 257 56 649.0 0 56 258 143 649.0 0 143 259 614 649.0 0 614 260 820 649.0 0 820 261 608 649.0 0 608 262 252 649.0 0 252 263 242 649.0 0 242 264 151 649.0 0 151 265 64 649.0 0 64 266 36 649.0 0 36 267 55 649.0 0 55 268 35 649.0 0 35 269 170 649.0 0 170 270 252 649.0 0 252 271 229 649.0 0 229 272 251 649.0 0 251 273 69 649.0 0 69 274 75 649.0 0 75 275 36 649.0 0 36 276 63 649.0 0 63 277 97 649.0 0 97 278 84 649.0 0 84 279 80 649.0 0 80 280 147 649.0 0 147 281 148 649.0 0 148 282 141 649.0 0 141 283 100 649.0 0 100 284 127 649.0 0 127 285 588 649.0 0 588 286 402 649.0 0 402 287 308 649.0 0 308 288 224 649.0 0 224 289 36 649.0 0 36 290 41 649.0 0 41 291 34 649.0 0 34 292 28 649.0 0 28 293 55 649.0 0 55 294 50 649.0 0 50 295 58 649.0 0 58 296 127 649.0 0 127 297 922 649.0 0 922 298 411 649.0 0 411 299 380 649.0 0 380 300 38 649.0 0 38 301 34 649.0 0 34 Finished in 17.30 s (38 us/read; 1.57 M reads/minute). === Summary === Total reads processed: 452,604 Reads with adapters: 41,513 (9.2%) Reads that were too short: 740 (0.2%) Reads written (passing filters): 40,773 (9.0%) Total basepairs processed: 104,199,123 bp Total written (filtered): 10,317,098 bp (9.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 41513 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 21 0.0 3 0 0 14 7 16 256 0.0 3 0 221 20 15 17 34509 0.0 3 32476 1696 294 43 18 111 0.0 3 9 83 11 8 19 111 0.0 3 3 0 58 50 20 80 0.0 3 6 1 0 73 23 3 0.0 3 0 0 0 3 25 1 0.0 3 1 26 1 0.0 3 1 28 4 0.0 3 0 0 1 3 29 1 0.0 3 0 0 0 1 30 13 0.0 3 0 0 0 13 31 5 0.0 3 0 0 0 5 32 12 0.0 3 0 0 1 11 42 1 0.0 3 0 0 0 1 45 2 0.0 3 0 0 0 2 47 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 51 2 0.0 3 0 0 0 2 52 6 0.0 3 0 0 0 6 53 1 0.0 3 0 0 0 1 55 5 0.0 3 0 0 0 5 56 2 0.0 3 0 0 0 2 64 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 74 172 0.0 3 0 0 0 172 75 9 0.0 3 0 0 0 9 76 15 0.0 3 0 0 0 15 77 40 0.0 3 0 0 0 40 78 3 0.0 3 0 0 0 3 79 31 0.0 3 0 0 0 31 80 6 0.0 3 0 0 0 6 81 3 0.0 3 0 0 1 2 82 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 85 2 0.0 3 0 0 0 2 89 1 0.0 3 0 1 90 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 98 50 0.0 3 0 0 1 49 99 3900 0.0 3 0 0 34 3866 100 822 0.0 3 0 0 7 815 101 5 0.0 3 0 0 0 5 102 355 0.0 3 0 0 13 342 103 152 0.0 3 0 0 5 147 105 4 0.0 3 0 0 0 4 106 1 0.0 3 0 0 0 1 107 3 0.0 3 0 0 0 3 108 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 122 6 0.0 3 0 0 0 6 123 10 0.0 3 0 0 1 9 124 3 0.0 3 0 0 0 3 125 29 0.0 3 0 0 0 29 126 7 0.0 3 0 0 1 6 127 1 0.0 3 0 0 0 1 132 3 0.0 3 0 0 0 3 134 11 0.0 3 0 0 0 11 135 9 0.0 3 0 0 1 8 137 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 144 4 0.0 3 3 0 1 146 2 0.0 3 0 0 0 2 147 3 0.0 3 0 0 0 3 149 2 0.0 3 0 0 0 2 150 49 0.0 3 0 0 1 48 151 20 0.0 3 0 0 0 20 152 3 0.0 3 0 0 0 3 153 6 0.0 3 0 0 0 6 154 1 0.0 3 0 0 0 1 156 3 0.0 3 0 0 0 3 158 3 0.0 3 0 0 0 3 159 36 0.0 3 0 0 0 36 160 20 0.0 3 0 0 2 18 161 3 0.0 3 0 0 1 2 162 3 0.0 3 0 0 0 3 163 1 0.0 3 0 0 0 1 164 3 0.0 3 0 0 0 3 166 1 0.0 3 0 0 0 1 169 3 0.0 3 0 0 1 2 170 1 0.0 3 0 0 0 1 171 4 0.0 3 0 0 0 4 172 3 0.0 3 0 0 0 3 174 6 0.0 3 0 0 0 6 175 10 0.0 3 0 0 0 10 176 7 0.0 3 0 0 0 7 177 3 0.0 3 0 0 0 3 178 2 0.0 3 0 0 0 2 181 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 188 2 0.0 3 0 0 0 2 189 5 0.0 3 0 0 0 5 190 9 0.0 3 0 0 0 9 191 42 0.0 3 0 0 0 42 192 5 0.0 3 0 0 0 5 193 2 0.0 3 0 0 0 2 194 4 0.0 3 0 0 1 3 195 1 0.0 3 0 0 0 1 199 8 0.0 3 0 0 1 7 200 200 0.0 3 0 0 7 193 201 68 0.0 3 0 0 2 66 202 28 0.0 3 0 0 1 27 203 2 0.0 3 0 0 0 2 204 3 0.0 3 0 0 0 3 205 2 0.0 3 0 0 0 2 207 2 0.0 3 0 0 0 2 208 1 0.0 3 0 0 0 1 209 5 0.0 3 0 0 0 5 210 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 213 3 0.0 3 0 0 0 3 215 2 0.0 3 0 0 0 2 217 8 0.0 3 0 0 1 7 218 2 0.0 3 0 0 0 2 224 1 0.0 3 0 0 0 1 225 2 0.0 3 0 0 0 2 239 6 0.0 3 0 0 0 6 240 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 0 1 251 1 0.0 3 0 0 0 1 267 2 0.0 3 0 0 0 2 268 1 0.0 3 0 0 0 1 271 2 0.0 3 0 0 0 2 274 1 0.0 3 0 0 0 1 275 2 0.0 3 0 0 0 2 276 7 0.0 3 0 0 0 7 277 5 0.0 3 0 0 0 5 278 16 0.0 3 0 0 0 16 279 9 0.0 3 0 0 0 9 280 4 0.0 3 0 0 0 4 281 7 0.0 3 0 0 0 7 283 3 0.0 3 0 0 0 3 285 1 0.0 3 0 0 0 1 286 1 0.0 3 0 0 0 1 289 2 0.0 3 0 0 0 2 291 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.36 s (33 us/read; 1.80 M reads/minute). === Summary === Total reads processed: 40,773 Reads with adapters: 372 (0.9%) Reads written (passing filters): 40,773 (100.0%) Total basepairs processed: 10,317,098 bp Total written (filtered): 10,315,938 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 372 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 31.5% C: 8.1% G: 53.5% T: 7.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 336 637.1 0 336 4 29 159.3 0 29 5 6 39.8 0 6 6 1 10.0 0 1