This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTCGGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 70.65 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 438,093 (16.5%) Reads that were too short: 25,924 (1.0%) Reads written (passing filters): 412,169 (15.5%) Total basepairs processed: 800,111,879 bp Total written (filtered): 96,747,722 bp (12.1%) === Adapter 1 === Sequence: TTCGGA; Type: regular 5'; Length: 6; Trimmed: 438093 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 590 649.0 0 590 7 2119 649.0 0 2119 8 36295 649.0 0 36295 9 42516 649.0 0 42516 10 61179 649.0 0 61179 11 50 649.0 0 50 12 13 649.0 0 13 13 16 649.0 0 16 14 14 649.0 0 14 15 15 649.0 0 15 16 21 649.0 0 21 17 26 649.0 0 26 18 13 649.0 0 13 19 35 649.0 0 35 20 22 649.0 0 22 21 30 649.0 0 30 22 31 649.0 0 31 23 44 649.0 0 44 24 59 649.0 0 59 25 33 649.0 0 33 26 56 649.0 0 56 27 66 649.0 0 66 28 30 649.0 0 30 29 34 649.0 0 34 30 31 649.0 0 31 31 37 649.0 0 37 32 54 649.0 0 54 33 32 649.0 0 32 34 19 649.0 0 19 35 35 649.0 0 35 36 35 649.0 0 35 37 16 649.0 0 16 38 30 649.0 0 30 39 59 649.0 0 59 40 50 649.0 0 50 41 157 649.0 0 157 42 34 649.0 0 34 43 27 649.0 0 27 44 12 649.0 0 12 45 21 649.0 0 21 46 24 649.0 0 24 47 32 649.0 0 32 48 103 649.0 0 103 49 61 649.0 0 61 50 58 649.0 0 58 51 32 649.0 0 32 52 63 649.0 0 63 53 105 649.0 0 105 54 109 649.0 0 109 55 41 649.0 0 41 56 36 649.0 0 36 57 75 649.0 0 75 58 610 649.0 0 610 59 34 649.0 0 34 60 60 649.0 0 60 61 64 649.0 0 64 62 73 649.0 0 73 63 68 649.0 0 68 64 25 649.0 0 25 65 48 649.0 0 48 66 75 649.0 0 75 67 52 649.0 0 52 68 152 649.0 0 152 69 83 649.0 0 83 70 59 649.0 0 59 71 108 649.0 0 108 72 151 649.0 0 151 73 97 649.0 0 97 74 87 649.0 0 87 75 199 649.0 0 199 76 270 649.0 0 270 77 266 649.0 0 266 78 683 649.0 0 683 79 1516 649.0 0 1516 80 345 649.0 0 345 81 488 649.0 0 488 82 1040 649.0 0 1040 83 463 649.0 0 463 84 436 649.0 0 436 85 1024 649.0 0 1024 86 1307 649.0 0 1307 87 1378 649.0 0 1378 88 926 649.0 0 926 89 1676 649.0 0 1676 90 2043 649.0 0 2043 91 3750 649.0 0 3750 92 79128 649.0 0 79128 93 42783 649.0 0 42783 94 111753 649.0 0 111753 95 775 649.0 0 775 96 28 649.0 0 28 97 42 649.0 0 42 98 28 649.0 0 28 99 28 649.0 0 28 100 14 649.0 0 14 101 24 649.0 0 24 102 28 649.0 0 28 103 16 649.0 0 16 104 16 649.0 0 16 105 13 649.0 0 13 106 15 649.0 0 15 107 19 649.0 0 19 108 22 649.0 0 22 109 37 649.0 0 37 110 29 649.0 0 29 111 31 649.0 0 31 112 64 649.0 0 64 113 19 649.0 0 19 114 24 649.0 0 24 115 28 649.0 0 28 116 45 649.0 0 45 117 80 649.0 0 80 118 39 649.0 0 39 119 19 649.0 0 19 120 13 649.0 0 13 121 13 649.0 0 13 122 10 649.0 0 10 123 13 649.0 0 13 124 20 649.0 0 20 125 25 649.0 0 25 126 16 649.0 0 16 127 35 649.0 0 35 128 21 649.0 0 21 129 23 649.0 0 23 130 26 649.0 0 26 131 18 649.0 0 18 132 24 649.0 0 24 133 16 649.0 0 16 134 17 649.0 0 17 135 19 649.0 0 19 136 22 649.0 0 22 137 41 649.0 0 41 138 30 649.0 0 30 139 12 649.0 0 12 140 14 649.0 0 14 141 19 649.0 0 19 142 20 649.0 0 20 143 23 649.0 0 23 144 18 649.0 0 18 145 29 649.0 0 29 146 18 649.0 0 18 147 31 649.0 0 31 148 29 649.0 0 29 149 17 649.0 0 17 150 21 649.0 0 21 151 26 649.0 0 26 152 16 649.0 0 16 153 20 649.0 0 20 154 25 649.0 0 25 155 11 649.0 0 11 156 22 649.0 0 22 157 28 649.0 0 28 158 31 649.0 0 31 159 23 649.0 0 23 160 17 649.0 0 17 161 27 649.0 0 27 162 16 649.0 0 16 163 24 649.0 0 24 164 18 649.0 0 18 165 19 649.0 0 19 166 19 649.0 0 19 167 25 649.0 0 25 168 24 649.0 0 24 169 19 649.0 0 19 170 15 649.0 0 15 171 37 649.0 0 37 172 28 649.0 0 28 173 30 649.0 0 30 174 51 649.0 0 51 175 42 649.0 0 42 176 78 649.0 0 78 177 241 649.0 0 241 178 1316 649.0 0 1316 179 1834 649.0 0 1834 180 812 649.0 0 812 181 24 649.0 0 24 182 22 649.0 0 22 183 34 649.0 0 34 184 33 649.0 0 33 185 35 649.0 0 35 186 47 649.0 0 47 187 80 649.0 0 80 188 137 649.0 0 137 189 63 649.0 0 63 190 127 649.0 0 127 191 160 649.0 0 160 192 80 649.0 0 80 193 54 649.0 0 54 194 112 649.0 0 112 195 145 649.0 0 145 196 123 649.0 0 123 197 118 649.0 0 118 198 145 649.0 0 145 199 202 649.0 0 202 200 283 649.0 0 283 201 5100 649.0 0 5100 202 3895 649.0 0 3895 203 7702 649.0 0 7702 204 208 649.0 0 208 205 492 649.0 0 492 206 740 649.0 0 740 207 852 649.0 0 852 208 227 649.0 0 227 209 174 649.0 0 174 210 128 649.0 0 128 211 75 649.0 0 75 212 71 649.0 0 71 213 35 649.0 0 35 214 41 649.0 0 41 215 64 649.0 0 64 216 64 649.0 0 64 217 78 649.0 0 78 218 79 649.0 0 79 219 26 649.0 0 26 220 48 649.0 0 48 221 224 649.0 0 224 222 224 649.0 0 224 223 364 649.0 0 364 224 148 649.0 0 148 225 266 649.0 0 266 226 448 649.0 0 448 227 662 649.0 0 662 228 539 649.0 0 539 229 569 649.0 0 569 230 20 649.0 0 20 231 10 649.0 0 10 232 21 649.0 0 21 233 27 649.0 0 27 234 17 649.0 0 17 235 34 649.0 0 34 236 58 649.0 0 58 237 55 649.0 0 55 238 45 649.0 0 45 239 22 649.0 0 22 240 10 649.0 0 10 241 27 649.0 0 27 242 18 649.0 0 18 243 31 649.0 0 31 244 24 649.0 0 24 245 33 649.0 0 33 246 35 649.0 0 35 247 37 649.0 0 37 248 31 649.0 0 31 249 42 649.0 0 42 250 23 649.0 0 23 251 29 649.0 0 29 252 19 649.0 0 19 253 26 649.0 0 26 254 18 649.0 0 18 255 23 649.0 0 23 256 29 649.0 0 29 257 48 649.0 0 48 258 98 649.0 0 98 259 605 649.0 0 605 260 727 649.0 0 727 261 719 649.0 0 719 262 334 649.0 0 334 263 321 649.0 0 321 264 164 649.0 0 164 265 26 649.0 0 26 266 28 649.0 0 28 267 27 649.0 0 27 268 19 649.0 0 19 269 61 649.0 0 61 270 95 649.0 0 95 271 122 649.0 0 122 272 91 649.0 0 91 273 44 649.0 0 44 274 39 649.0 0 39 275 18 649.0 0 18 276 74 649.0 0 74 277 91 649.0 0 91 278 63 649.0 0 63 279 56 649.0 0 56 280 105 649.0 0 105 281 96 649.0 0 96 282 82 649.0 0 82 283 65 649.0 0 65 284 100 649.0 0 100 285 420 649.0 0 420 286 256 649.0 0 256 287 206 649.0 0 206 288 156 649.0 0 156 289 24 649.0 0 24 290 23 649.0 0 23 291 39 649.0 0 39 292 25 649.0 0 25 293 46 649.0 0 46 294 32 649.0 0 32 295 33 649.0 0 33 296 92 649.0 0 92 297 580 649.0 0 580 298 202 649.0 0 202 299 216 649.0 0 216 300 35 649.0 0 35 301 14 649.0 0 14 Finished in 18.02 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 412,169 Reads with adapters: 37,844 (9.2%) Reads that were too short: 41 (0.0%) Reads written (passing filters): 37,803 (9.2%) Total basepairs processed: 96,747,722 bp Total written (filtered): 10,141,443 bp (10.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 37844 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 19 0.0 3 0 0 18 1 19 680 0.0 3 0 666 13 1 20 35643 0.0 4 34909 704 25 4 1 21 68 0.0 4 11 54 3 22 8 0.0 4 1 0 5 2 23 25 0.0 4 1 0 0 22 2 24 1 0.0 4 0 0 0 1 29 1 0.0 4 1 32 1 0.0 4 1 34 1 0.0 4 1 39 1 0.0 4 1 42 2 0.0 4 0 0 0 0 2 43 4 0.0 4 0 0 0 0 4 45 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 67 1 0.0 4 0 0 0 0 1 68 20 0.0 4 0 0 0 0 20 78 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 94 2 0.0 4 0 0 0 0 2 109 1 0.0 4 0 0 0 0 1 120 1 0.0 4 1 121 2 0.0 4 0 0 0 1 1 124 1 0.0 4 0 0 0 0 1 125 3 0.0 4 0 0 0 0 3 126 11 0.0 4 0 0 0 0 11 127 1305 0.0 4 0 0 0 0 1305 128 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 134 1 0.0 4 1 136 3 0.0 4 0 0 0 0 3 138 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 149 2 0.0 4 0 0 0 0 2 150 4 0.0 4 0 0 0 0 4 151 1 0.0 4 0 0 0 0 1 155 1 0.0 4 0 0 0 0 1 161 1 0.0 4 1 167 1 0.0 4 1 168 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 0 0 0 1 173 1 0.0 4 0 0 0 0 1 197 3 0.0 4 0 0 0 0 3 198 2 0.0 4 0 0 0 0 2 200 1 0.0 4 1 204 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 0 3 229 2 0.0 4 0 0 0 0 2 232 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 251 1 0.0 4 0 0 1 254 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.32 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 37,803 Reads with adapters: 4,152 (11.0%) Reads written (passing filters): 37,803 (100.0%) Total basepairs processed: 10,141,443 bp Total written (filtered): 10,100,229 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 4152 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.3% C: 3.1% G: 93.7% T: 1.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 12 590.7 0 12 4 5 147.7 0 5 5 2 36.9 0 2 6 1 9.2 0 1 9 1534 0.1 0 51 1483 10 1830 0.0 1 68 1762 11 757 0.0 1 6 751 12 5 0.0 1 0 5 13 3 0.0 1 0 3 152 1 0.0 1 0 1 223 1 0.0 1 1 235 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.