This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGAGAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.98 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 367,328 (13.8%) Reads that were too short: 20,298 (0.8%) Reads written (passing filters): 347,030 (13.1%) Total basepairs processed: 800,111,879 bp Total written (filtered): 88,855,553 bp (11.1%) === Adapter 1 === Sequence: AGAGAC; Type: regular 5'; Length: 6; Trimmed: 367328 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2440 649.0 0 2440 7 96153 649.0 0 96153 8 85014 649.0 0 85014 9 86 649.0 0 86 10 268 649.0 0 268 11 44 649.0 0 44 12 26 649.0 0 26 13 40 649.0 0 40 14 30 649.0 0 30 15 3 649.0 0 3 16 5 649.0 0 5 17 7 649.0 0 7 18 6 649.0 0 6 19 15 649.0 0 15 20 15 649.0 0 15 21 25 649.0 0 25 22 24 649.0 0 24 23 22 649.0 0 22 24 16 649.0 0 16 25 21 649.0 0 21 26 15 649.0 0 15 27 15 649.0 0 15 28 49 649.0 0 49 29 102 649.0 0 102 30 28 649.0 0 28 31 16 649.0 0 16 32 16 649.0 0 16 33 64 649.0 0 64 34 64 649.0 0 64 35 48 649.0 0 48 36 57 649.0 0 57 37 28 649.0 0 28 38 15 649.0 0 15 39 16 649.0 0 16 40 18 649.0 0 18 41 15 649.0 0 15 42 50 649.0 0 50 43 44 649.0 0 44 44 52 649.0 0 52 45 37 649.0 0 37 46 62 649.0 0 62 47 43 649.0 0 43 48 35 649.0 0 35 49 58 649.0 0 58 50 48 649.0 0 48 51 69 649.0 0 69 52 224 649.0 0 224 53 81 649.0 0 81 54 73 649.0 0 73 55 103 649.0 0 103 56 32 649.0 0 32 57 15 649.0 0 15 58 12 649.0 0 12 59 49 649.0 0 49 60 178 649.0 0 178 61 127 649.0 0 127 62 355 649.0 0 355 63 173 649.0 0 173 64 232 649.0 0 232 65 887 649.0 0 887 66 676 649.0 0 676 67 1556 649.0 0 1556 68 142 649.0 0 142 69 113 649.0 0 113 70 76 649.0 0 76 71 125 649.0 0 125 72 449 649.0 0 449 73 350 649.0 0 350 74 671 649.0 0 671 75 987 649.0 0 987 76 1034 649.0 0 1034 77 2395 649.0 0 2395 78 3993 649.0 0 3993 79 53528 649.0 0 53528 80 26893 649.0 0 26893 81 44669 649.0 0 44669 82 514 649.0 0 514 83 373 649.0 0 373 84 103 649.0 0 103 85 90 649.0 0 90 86 117 649.0 0 117 87 141 649.0 0 141 88 79 649.0 0 79 89 64 649.0 0 64 90 128 649.0 0 128 91 32 649.0 0 32 92 87 649.0 0 87 93 72 649.0 0 72 94 50 649.0 0 50 95 94 649.0 0 94 96 65 649.0 0 65 97 30 649.0 0 30 98 31 649.0 0 31 99 47 649.0 0 47 100 33 649.0 0 33 101 50 649.0 0 50 102 57 649.0 0 57 103 51 649.0 0 51 104 40 649.0 0 40 105 33 649.0 0 33 106 33 649.0 0 33 107 22 649.0 0 22 108 35 649.0 0 35 109 34 649.0 0 34 110 45 649.0 0 45 111 68 649.0 0 68 112 67 649.0 0 67 113 88 649.0 0 88 114 95 649.0 0 95 115 121 649.0 0 121 116 409 649.0 0 409 117 315 649.0 0 315 118 779 649.0 0 779 119 223 649.0 0 223 120 200 649.0 0 200 121 180 649.0 0 180 122 146 649.0 0 146 123 227 649.0 0 227 124 168 649.0 0 168 125 243 649.0 0 243 126 264 649.0 0 264 127 243 649.0 0 243 128 117 649.0 0 117 129 156 649.0 0 156 130 260 649.0 0 260 131 283 649.0 0 283 132 372 649.0 0 372 133 160 649.0 0 160 134 132 649.0 0 132 135 344 649.0 0 344 136 107 649.0 0 107 137 261 649.0 0 261 138 128 649.0 0 128 139 159 649.0 0 159 140 386 649.0 0 386 141 240 649.0 0 240 142 330 649.0 0 330 143 152 649.0 0 152 144 272 649.0 0 272 145 223 649.0 0 223 146 198 649.0 0 198 147 231 649.0 0 231 148 183 649.0 0 183 149 212 649.0 0 212 150 852 649.0 0 852 151 579 649.0 0 579 152 1972 649.0 0 1972 153 219 649.0 0 219 154 122 649.0 0 122 155 107 649.0 0 107 156 99 649.0 0 99 157 115 649.0 0 115 158 129 649.0 0 129 159 146 649.0 0 146 160 102 649.0 0 102 161 95 649.0 0 95 162 99 649.0 0 99 163 111 649.0 0 111 164 75 649.0 0 75 165 114 649.0 0 114 166 115 649.0 0 115 167 154 649.0 0 154 168 135 649.0 0 135 169 150 649.0 0 150 170 184 649.0 0 184 171 166 649.0 0 166 172 234 649.0 0 234 173 378 649.0 0 378 174 244 649.0 0 244 175 102 649.0 0 102 176 91 649.0 0 91 177 109 649.0 0 109 178 108 649.0 0 108 179 120 649.0 0 120 180 110 649.0 0 110 181 111 649.0 0 111 182 129 649.0 0 129 183 106 649.0 0 106 184 120 649.0 0 120 185 115 649.0 0 115 186 217 649.0 0 217 187 181 649.0 0 181 188 138 649.0 0 138 189 116 649.0 0 116 190 102 649.0 0 102 191 127 649.0 0 127 192 122 649.0 0 122 193 142 649.0 0 142 194 123 649.0 0 123 195 132 649.0 0 132 196 103 649.0 0 103 197 148 649.0 0 148 198 156 649.0 0 156 199 257 649.0 0 257 200 131 649.0 0 131 201 104 649.0 0 104 202 119 649.0 0 119 203 98 649.0 0 98 204 119 649.0 0 119 205 209 649.0 0 209 206 129 649.0 0 129 207 90 649.0 0 90 208 109 649.0 0 109 209 104 649.0 0 104 210 158 649.0 0 158 211 105 649.0 0 105 212 135 649.0 0 135 213 125 649.0 0 125 214 125 649.0 0 125 215 145 649.0 0 145 216 171 649.0 0 171 217 669 649.0 0 669 218 542 649.0 0 542 219 453 649.0 0 453 220 147 649.0 0 147 221 166 649.0 0 166 222 203 649.0 0 203 223 227 649.0 0 227 224 207 649.0 0 207 225 180 649.0 0 180 226 705 649.0 0 705 227 624 649.0 0 624 228 1847 649.0 0 1847 229 201 649.0 0 201 230 140 649.0 0 140 231 99 649.0 0 99 232 117 649.0 0 117 233 96 649.0 0 96 234 82 649.0 0 82 235 95 649.0 0 95 236 104 649.0 0 104 237 94 649.0 0 94 238 125 649.0 0 125 239 133 649.0 0 133 240 111 649.0 0 111 241 145 649.0 0 145 242 122 649.0 0 122 243 144 649.0 0 144 244 164 649.0 0 164 245 137 649.0 0 137 246 225 649.0 0 225 247 203 649.0 0 203 248 172 649.0 0 172 249 182 649.0 0 182 250 178 649.0 0 178 251 164 649.0 0 164 252 174 649.0 0 174 253 229 649.0 0 229 254 125 649.0 0 125 255 138 649.0 0 138 256 142 649.0 0 142 257 141 649.0 0 141 258 101 649.0 0 101 259 102 649.0 0 102 260 112 649.0 0 112 261 120 649.0 0 120 262 157 649.0 0 157 263 169 649.0 0 169 264 162 649.0 0 162 265 149 649.0 0 149 266 214 649.0 0 214 267 142 649.0 0 142 268 160 649.0 0 160 269 121 649.0 0 121 270 132 649.0 0 132 271 186 649.0 0 186 272 183 649.0 0 183 273 168 649.0 0 168 274 241 649.0 0 241 275 239 649.0 0 239 276 216 649.0 0 216 277 185 649.0 0 185 278 180 649.0 0 180 279 286 649.0 0 286 280 224 649.0 0 224 281 270 649.0 0 270 282 187 649.0 0 187 283 152 649.0 0 152 284 129 649.0 0 129 285 127 649.0 0 127 286 184 649.0 0 184 287 191 649.0 0 191 288 186 649.0 0 186 289 192 649.0 0 192 290 242 649.0 0 242 291 240 649.0 0 240 292 261 649.0 0 261 293 209 649.0 0 209 294 203 649.0 0 203 295 159 649.0 0 159 296 166 649.0 0 166 297 181 649.0 0 181 298 246 649.0 0 246 299 219 649.0 0 219 300 333 649.0 0 333 301 179 649.0 0 179 Finished in 14.53 s (42 us/read; 1.43 M reads/minute). === Summary === Total reads processed: 347,030 Reads with adapters: 93,903 (27.1%) Reads that were too short: 445 (0.1%) Reads written (passing filters): 93,458 (26.9%) Total basepairs processed: 88,855,553 bp Total written (filtered): 25,819,403 bp (29.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 93903 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 11 0.0 2 0 0 0 11 15 37 0.0 3 0 0 23 14 16 752 0.0 3 0 647 60 45 17 91657 0.0 3 86839 4035 623 160 18 187 0.0 3 10 151 12 14 19 229 0.0 3 2 1 124 102 20 141 0.0 3 0 2 0 139 21 2 0.0 3 1 0 0 1 22 4 0.0 3 0 0 0 4 23 5 0.0 3 0 0 0 5 24 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 26 13 0.0 3 0 0 1 12 27 1 0.0 3 0 0 0 1 28 5 0.0 3 1 0 0 4 29 4 0.0 3 0 1 0 3 31 10 0.0 3 0 0 0 10 32 1 0.0 3 1 34 1 0.0 3 1 35 2 0.0 3 0 0 0 2 37 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 41 73 0.0 3 0 0 1 72 44 13 0.0 3 0 0 0 13 46 2 0.0 3 0 0 0 2 47 9 0.0 3 0 0 1 8 48 3 0.0 3 1 0 0 2 49 6 0.0 3 0 0 1 5 50 1 0.0 3 0 0 0 1 51 2 0.0 3 0 0 0 2 52 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 55 3 0.0 3 0 0 0 3 56 4 0.0 3 0 0 0 4 57 9 0.0 3 0 0 0 9 58 5 0.0 3 0 0 0 5 59 3 0.0 3 0 0 0 3 62 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 65 2 0.0 3 0 1 0 1 66 2 0.0 3 0 0 0 2 67 7 0.0 3 0 0 1 6 70 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 72 4 0.0 3 0 0 0 4 73 2 0.0 3 0 0 0 2 74 2 0.0 3 0 0 0 2 75 9 0.0 3 0 0 0 9 76 16 0.0 3 0 0 0 16 77 1 0.0 3 0 0 0 1 78 2 0.0 3 0 1 0 1 79 4 0.0 3 0 0 0 4 80 1 0.0 3 0 0 0 1 81 3 0.0 3 0 0 0 3 82 1 0.0 3 0 0 0 1 83 2 0.0 3 0 0 0 2 85 1 0.0 3 0 0 0 1 86 3 0.0 3 0 0 0 3 87 3 0.0 3 0 0 0 3 88 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 1 94 1 0.0 3 0 0 0 1 97 1 0.0 3 0 1 98 1 0.0 3 0 0 0 1 99 2 0.0 3 0 0 0 2 100 1 0.0 3 0 0 0 1 101 13 0.0 3 0 0 0 13 102 1 0.0 3 0 0 0 1 104 2 0.0 3 0 0 0 2 106 28 0.0 3 0 0 1 27 107 18 0.0 3 0 0 0 18 108 6 0.0 3 0 0 0 6 109 12 0.0 3 0 0 0 12 110 5 0.0 3 0 0 0 5 111 1 0.0 3 0 0 0 1 112 7 0.0 3 0 0 0 7 113 2 0.0 3 0 0 0 2 114 1 0.0 3 0 0 0 1 116 2 0.0 3 0 0 1 1 117 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 120 4 0.0 3 0 0 1 3 121 1 0.0 3 0 0 0 1 122 5 0.0 3 0 0 2 3 123 1 0.0 3 0 0 0 1 124 2 0.0 3 0 0 0 2 125 27 0.0 3 0 0 2 25 126 4 0.0 3 0 0 1 3 127 17 0.0 3 0 0 0 17 128 6 0.0 3 0 0 0 6 130 3 0.0 3 0 0 0 3 131 2 0.0 3 0 0 0 2 132 4 0.0 3 0 0 0 4 133 24 0.0 3 1 0 1 22 134 6 0.0 3 0 0 0 6 135 1 0.0 3 0 0 0 1 136 3 0.0 3 1 0 0 2 138 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 140 4 0.0 3 0 0 0 4 141 1 0.0 3 0 0 0 1 142 3 0.0 3 0 0 0 3 144 19 0.0 3 0 0 0 19 145 5 0.0 3 0 0 1 4 146 14 0.0 3 0 0 1 13 149 36 0.0 3 0 0 0 36 150 6 0.0 3 0 0 1 5 151 1 0.0 3 0 0 0 1 152 2 0.0 3 0 0 0 2 153 2 0.0 3 0 0 0 2 154 2 0.0 3 0 0 0 2 155 1 0.0 3 0 0 0 1 156 3 0.0 3 0 0 0 3 157 4 0.0 3 0 0 0 4 158 2 0.0 3 0 0 0 2 159 1 0.0 3 0 0 0 1 161 8 0.0 3 0 0 1 7 162 2 0.0 3 0 0 0 2 164 11 0.0 3 0 0 0 11 165 3 0.0 3 0 0 0 3 166 4 0.0 3 0 0 0 4 167 2 0.0 3 0 0 0 2 168 2 0.0 3 0 0 0 2 169 5 0.0 3 0 0 0 5 170 2 0.0 3 0 0 0 2 171 5 0.0 3 0 0 0 5 172 3 0.0 3 0 0 0 3 173 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 182 4 0.0 3 0 0 0 4 183 5 0.0 3 0 0 0 5 184 2 0.0 3 0 0 0 2 185 2 0.0 3 0 0 1 1 186 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 190 2 0.0 3 0 0 0 2 192 4 0.0 3 0 0 0 4 197 2 0.0 3 0 0 0 2 200 1 0.0 3 0 0 0 1 203 3 0.0 3 0 0 1 2 204 1 0.0 3 0 0 0 1 205 4 0.0 3 0 0 0 4 207 22 0.0 3 0 0 0 22 208 21 0.0 3 0 0 0 21 209 34 0.0 3 0 0 2 32 210 33 0.0 3 0 0 1 32 211 11 0.0 3 0 0 1 10 214 1 0.0 3 0 0 0 1 216 2 0.0 3 0 0 0 2 218 1 0.0 3 0 0 0 1 219 10 0.0 3 0 0 0 10 221 3 0.0 3 0 0 0 3 222 3 0.0 3 0 0 0 3 223 3 0.0 3 0 0 0 3 224 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 226 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 236 2 0.0 3 0 0 0 2 238 2 0.0 3 0 0 0 2 249 1 0.0 3 0 0 0 1 250 1 0.0 3 0 0 1 260 1 0.0 3 0 0 0 1 262 1 0.0 3 0 0 0 1 266 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 10 0.0 3 0 0 0 10 280 7 0.0 3 0 0 0 7 281 17 0.0 3 0 0 1 16 282 8 0.0 3 0 0 0 8 283 4 0.0 3 0 0 0 4 284 3 0.0 3 0 0 0 3 286 2 0.0 3 0 0 0 2 287 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.29 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 93,458 Reads with adapters: 1,078 (1.2%) Reads written (passing filters): 93,458 (100.0%) Total basepairs processed: 25,819,403 bp Total written (filtered): 25,816,116 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1078 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 20.1% C: 26.3% G: 41.7% T: 11.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1041 1460.3 0 1041 4 24 365.1 0 24 5 10 91.3 0 10 6 3 22.8 0 3