This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGAGAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 70.20 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 385,615 (14.5%) Reads that were too short: 15,019 (0.6%) Reads written (passing filters): 370,596 (13.9%) Total basepairs processed: 800,111,879 bp Total written (filtered): 94,691,049 bp (11.8%) === Adapter 1 === Sequence: AGAGAC; Type: regular 5'; Length: 6; Trimmed: 385615 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1288 649.0 0 1288 7 92193 649.0 0 92193 8 94815 649.0 0 94815 9 78 649.0 0 78 10 95 649.0 0 95 11 46 649.0 0 46 12 59 649.0 0 59 13 32 649.0 0 32 14 6 649.0 0 6 15 2 649.0 0 2 16 6 649.0 0 6 17 9 649.0 0 9 18 7 649.0 0 7 19 11 649.0 0 11 20 17 649.0 0 17 21 35 649.0 0 35 22 26 649.0 0 26 23 21 649.0 0 21 24 6 649.0 0 6 25 14 649.0 0 14 26 6 649.0 0 6 27 16 649.0 0 16 28 61 649.0 0 61 29 100 649.0 0 100 30 24 649.0 0 24 31 5 649.0 0 5 32 15 649.0 0 15 33 61 649.0 0 61 34 63 649.0 0 63 35 38 649.0 0 38 36 43 649.0 0 43 37 26 649.0 0 26 38 12 649.0 0 12 39 29 649.0 0 29 40 28 649.0 0 28 41 11 649.0 0 11 42 59 649.0 0 59 43 53 649.0 0 53 44 46 649.0 0 46 45 62 649.0 0 62 46 73 649.0 0 73 47 67 649.0 0 67 48 29 649.0 0 29 49 66 649.0 0 66 50 53 649.0 0 53 51 81 649.0 0 81 52 299 649.0 0 299 53 47 649.0 0 47 54 52 649.0 0 52 55 85 649.0 0 85 56 36 649.0 0 36 57 18 649.0 0 18 58 26 649.0 0 26 59 61 649.0 0 61 60 195 649.0 0 195 61 113 649.0 0 113 62 335 649.0 0 335 63 87 649.0 0 87 64 158 649.0 0 158 65 510 649.0 0 510 66 371 649.0 0 371 67 821 649.0 0 821 68 142 649.0 0 142 69 123 649.0 0 123 70 72 649.0 0 72 71 156 649.0 0 156 72 310 649.0 0 310 73 333 649.0 0 333 74 466 649.0 0 466 75 935 649.0 0 935 76 1089 649.0 0 1089 77 2290 649.0 0 2290 78 4022 649.0 0 4022 79 63896 649.0 0 63896 80 33914 649.0 0 33914 81 53484 649.0 0 53484 82 425 649.0 0 425 83 214 649.0 0 214 84 110 649.0 0 110 85 62 649.0 0 62 86 76 649.0 0 76 87 88 649.0 0 88 88 67 649.0 0 67 89 38 649.0 0 38 90 42 649.0 0 42 91 48 649.0 0 48 92 44 649.0 0 44 93 59 649.0 0 59 94 62 649.0 0 62 95 44 649.0 0 44 96 39 649.0 0 39 97 44 649.0 0 44 98 39 649.0 0 39 99 55 649.0 0 55 100 58 649.0 0 58 101 53 649.0 0 53 102 58 649.0 0 58 103 26 649.0 0 26 104 41 649.0 0 41 105 46 649.0 0 46 106 38 649.0 0 38 107 29 649.0 0 29 108 34 649.0 0 34 109 49 649.0 0 49 110 53 649.0 0 53 111 62 649.0 0 62 112 52 649.0 0 52 113 67 649.0 0 67 114 62 649.0 0 62 115 89 649.0 0 89 116 211 649.0 0 211 117 171 649.0 0 171 118 710 649.0 0 710 119 214 649.0 0 214 120 207 649.0 0 207 121 108 649.0 0 108 122 113 649.0 0 113 123 163 649.0 0 163 124 81 649.0 0 81 125 139 649.0 0 139 126 111 649.0 0 111 127 140 649.0 0 140 128 71 649.0 0 71 129 106 649.0 0 106 130 204 649.0 0 204 131 146 649.0 0 146 132 273 649.0 0 273 133 101 649.0 0 101 134 140 649.0 0 140 135 297 649.0 0 297 136 87 649.0 0 87 137 212 649.0 0 212 138 81 649.0 0 81 139 114 649.0 0 114 140 342 649.0 0 342 141 215 649.0 0 215 142 278 649.0 0 278 143 117 649.0 0 117 144 186 649.0 0 186 145 136 649.0 0 136 146 118 649.0 0 118 147 146 649.0 0 146 148 129 649.0 0 129 149 167 649.0 0 167 150 622 649.0 0 622 151 440 649.0 0 440 152 1652 649.0 0 1652 153 120 649.0 0 120 154 89 649.0 0 89 155 71 649.0 0 71 156 91 649.0 0 91 157 87 649.0 0 87 158 103 649.0 0 103 159 111 649.0 0 111 160 77 649.0 0 77 161 63 649.0 0 63 162 59 649.0 0 59 163 66 649.0 0 66 164 65 649.0 0 65 165 82 649.0 0 82 166 89 649.0 0 89 167 120 649.0 0 120 168 83 649.0 0 83 169 114 649.0 0 114 170 140 649.0 0 140 171 119 649.0 0 119 172 169 649.0 0 169 173 323 649.0 0 323 174 175 649.0 0 175 175 69 649.0 0 69 176 68 649.0 0 68 177 74 649.0 0 74 178 97 649.0 0 97 179 86 649.0 0 86 180 70 649.0 0 70 181 78 649.0 0 78 182 75 649.0 0 75 183 82 649.0 0 82 184 94 649.0 0 94 185 90 649.0 0 90 186 167 649.0 0 167 187 116 649.0 0 116 188 134 649.0 0 134 189 84 649.0 0 84 190 63 649.0 0 63 191 95 649.0 0 95 192 69 649.0 0 69 193 89 649.0 0 89 194 97 649.0 0 97 195 106 649.0 0 106 196 85 649.0 0 85 197 118 649.0 0 118 198 126 649.0 0 126 199 296 649.0 0 296 200 100 649.0 0 100 201 92 649.0 0 92 202 67 649.0 0 67 203 75 649.0 0 75 204 95 649.0 0 95 205 176 649.0 0 176 206 150 649.0 0 150 207 63 649.0 0 63 208 70 649.0 0 70 209 95 649.0 0 95 210 125 649.0 0 125 211 98 649.0 0 98 212 108 649.0 0 108 213 105 649.0 0 105 214 110 649.0 0 110 215 81 649.0 0 81 216 140 649.0 0 140 217 782 649.0 0 782 218 613 649.0 0 613 219 475 649.0 0 475 220 146 649.0 0 146 221 107 649.0 0 107 222 156 649.0 0 156 223 149 649.0 0 149 224 123 649.0 0 123 225 131 649.0 0 131 226 574 649.0 0 574 227 432 649.0 0 432 228 2168 649.0 0 2168 229 92 649.0 0 92 230 85 649.0 0 85 231 86 649.0 0 86 232 112 649.0 0 112 233 77 649.0 0 77 234 63 649.0 0 63 235 57 649.0 0 57 236 74 649.0 0 74 237 80 649.0 0 80 238 85 649.0 0 85 239 112 649.0 0 112 240 105 649.0 0 105 241 98 649.0 0 98 242 81 649.0 0 81 243 81 649.0 0 81 244 89 649.0 0 89 245 98 649.0 0 98 246 108 649.0 0 108 247 90 649.0 0 90 248 97 649.0 0 97 249 92 649.0 0 92 250 93 649.0 0 93 251 110 649.0 0 110 252 114 649.0 0 114 253 180 649.0 0 180 254 82 649.0 0 82 255 74 649.0 0 74 256 80 649.0 0 80 257 122 649.0 0 122 258 84 649.0 0 84 259 69 649.0 0 69 260 69 649.0 0 69 261 85 649.0 0 85 262 86 649.0 0 86 263 91 649.0 0 91 264 71 649.0 0 71 265 93 649.0 0 93 266 113 649.0 0 113 267 63 649.0 0 63 268 101 649.0 0 101 269 61 649.0 0 61 270 127 649.0 0 127 271 179 649.0 0 179 272 96 649.0 0 96 273 129 649.0 0 129 274 163 649.0 0 163 275 223 649.0 0 223 276 116 649.0 0 116 277 92 649.0 0 92 278 86 649.0 0 86 279 135 649.0 0 135 280 108 649.0 0 108 281 150 649.0 0 150 282 108 649.0 0 108 283 78 649.0 0 78 284 84 649.0 0 84 285 71 649.0 0 71 286 117 649.0 0 117 287 112 649.0 0 112 288 83 649.0 0 83 289 101 649.0 0 101 290 144 649.0 0 144 291 139 649.0 0 139 292 141 649.0 0 141 293 110 649.0 0 110 294 97 649.0 0 97 295 102 649.0 0 102 296 95 649.0 0 95 297 136 649.0 0 136 298 151 649.0 0 151 299 136 649.0 0 136 300 177 649.0 0 177 301 116 649.0 0 116 Finished in 17.18 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 370,596 Reads with adapters: 100,316 (27.1%) Reads that were too short: 59 (0.0%) Reads written (passing filters): 100,257 (27.1%) Total basepairs processed: 94,691,049 bp Total written (filtered): 27,272,350 bp (28.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 100316 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 0 3 18 32 0.0 3 0 0 30 2 19 870 0.0 3 0 839 27 2 2 20 95071 0.0 4 93381 1641 46 3 21 239 0.0 4 39 195 5 22 3027 0.0 4 2948 63 16 23 101 0.0 4 2 4 0 76 19 25 2 0.0 4 0 0 0 2 27 1 0.0 4 1 29 2 0.0 4 0 0 0 0 2 42 1 0.0 4 0 0 0 0 1 43 1 0.0 4 0 0 0 0 1 50 1 0.0 4 1 55 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 68 1 0.0 4 0 1 91 2 0.0 4 0 0 0 0 2 93 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 125 4 0.0 4 0 0 0 0 4 126 8 0.0 4 0 0 0 0 8 127 883 0.0 4 0 0 0 0 883 128 6 0.0 4 0 0 0 0 6 129 3 0.0 4 0 1 0 0 2 131 1 0.0 4 0 0 0 0 1 141 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 1 153 2 0.0 4 0 0 0 0 2 170 1 0.0 4 0 0 0 0 1 174 1 0.0 4 0 0 0 0 1 181 1 0.0 4 0 0 0 0 1 184 1 0.0 4 0 0 0 0 1 187 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 1 202 1 0.0 4 0 0 0 0 1 205 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 1 218 2 0.0 4 0 0 0 0 2 228 18 0.0 4 0 0 0 1 17 229 6 0.0 4 0 0 0 1 5 230 1 0.0 4 0 0 0 1 235 2 0.0 4 0 0 0 0 2 262 2 0.0 4 0 0 0 0 2 263 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.53 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 100,257 Reads with adapters: 6,534 (6.5%) Reads written (passing filters): 100,257 (100.0%) Total basepairs processed: 27,272,350 bp Total written (filtered): 27,198,406 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 6534 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.5% C: 2.9% G: 92.4% T: 2.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 78 1566.5 0 78 4 16 391.6 0 16 5 3 97.9 0 3 6 24 24.5 0 24 9 255 0.4 0 8 247 10 1268 0.1 1 47 1221 11 4801 0.0 1 28 4773 12 15 0.0 1 1 14 13 1 0.0 1 0 1 14 5 0.0 1 0 5 15 2 0.0 1 0 2 18 15 0.0 1 12 3 19 3 0.0 1 3 21 3 0.0 1 2 1 22 12 0.0 1 10 2 23 1 0.0 1 1 35 1 0.0 1 0 1 42 1 0.0 1 0 1 50 1 0.0 1 1 71 1 0.0 1 1 83 1 0.0 1 1 84 1 0.0 1 1 92 1 0.0 1 1 151 2 0.0 1 0 2 156 16 0.0 1 14 2 170 1 0.0 1 0 1 171 1 0.0 1 1 221 1 0.0 1 0 1 223 2 0.0 1 2 229 1 0.0 1 1 239 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.