This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGAATC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 70.13 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 57,075 (2.1%) Reads that were too short: 5,281 (0.2%) Reads written (passing filters): 51,794 (1.9%) Total basepairs processed: 800,111,879 bp Total written (filtered): 13,007,460 bp (1.6%) === Adapter 1 === Sequence: CGAATC; Type: regular 5'; Length: 6; Trimmed: 57075 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 994 649.0 0 994 7 13100 649.0 0 13100 8 302 649.0 0 302 9 8838 649.0 0 8838 10 22 649.0 0 22 11 17 649.0 0 17 12 14 649.0 0 14 13 18 649.0 0 18 14 14 649.0 0 14 15 17 649.0 0 17 16 15 649.0 0 15 17 26 649.0 0 26 18 19 649.0 0 19 19 19 649.0 0 19 20 18 649.0 0 18 21 11 649.0 0 11 22 16 649.0 0 16 23 28 649.0 0 28 24 30 649.0 0 30 25 20 649.0 0 20 26 13 649.0 0 13 27 36 649.0 0 36 28 49 649.0 0 49 29 31 649.0 0 31 30 38 649.0 0 38 31 17 649.0 0 17 32 18 649.0 0 18 33 57 649.0 0 57 34 84 649.0 0 84 35 124 649.0 0 124 36 25 649.0 0 25 37 42 649.0 0 42 38 26 649.0 0 26 39 25 649.0 0 25 40 36 649.0 0 36 41 26 649.0 0 26 42 31 649.0 0 31 43 46 649.0 0 46 44 80 649.0 0 80 45 62 649.0 0 62 46 38 649.0 0 38 47 32 649.0 0 32 48 50 649.0 0 50 49 45 649.0 0 45 50 103 649.0 0 103 51 96 649.0 0 96 52 161 649.0 0 161 53 237 649.0 0 237 54 3429 649.0 0 3429 55 4726 649.0 0 4726 56 4203 649.0 0 4203 57 31 649.0 0 31 58 17 649.0 0 17 59 16 649.0 0 16 60 19 649.0 0 19 61 19 649.0 0 19 62 16 649.0 0 16 63 19 649.0 0 19 64 17 649.0 0 17 65 19 649.0 0 19 66 17 649.0 0 17 67 19 649.0 0 19 68 21 649.0 0 21 69 31 649.0 0 31 70 23 649.0 0 23 71 19 649.0 0 19 72 18 649.0 0 18 73 19 649.0 0 19 74 22 649.0 0 22 75 26 649.0 0 26 76 12 649.0 0 12 77 26 649.0 0 26 78 12 649.0 0 12 79 22 649.0 0 22 80 38 649.0 0 38 81 67 649.0 0 67 82 39 649.0 0 39 83 37 649.0 0 37 84 47 649.0 0 47 85 23 649.0 0 23 86 55 649.0 0 55 87 54 649.0 0 54 88 67 649.0 0 67 89 48 649.0 0 48 90 44 649.0 0 44 91 79 649.0 0 79 92 70 649.0 0 70 93 142 649.0 0 142 94 3916 649.0 0 3916 95 998 649.0 0 998 96 2241 649.0 0 2241 97 30 649.0 0 30 98 24 649.0 0 24 99 26 649.0 0 26 100 26 649.0 0 26 101 22 649.0 0 22 102 24 649.0 0 24 103 31 649.0 0 31 104 24 649.0 0 24 105 36 649.0 0 36 106 19 649.0 0 19 107 28 649.0 0 28 108 23 649.0 0 23 109 102 649.0 0 102 110 86 649.0 0 86 111 151 649.0 0 151 112 16 649.0 0 16 113 29 649.0 0 29 114 35 649.0 0 35 115 32 649.0 0 32 116 30 649.0 0 30 117 49 649.0 0 49 118 45 649.0 0 45 119 59 649.0 0 59 120 68 649.0 0 68 121 286 649.0 0 286 122 215 649.0 0 215 123 233 649.0 0 233 124 20 649.0 0 20 125 40 649.0 0 40 126 37 649.0 0 37 127 32 649.0 0 32 128 30 649.0 0 30 129 37 649.0 0 37 130 38 649.0 0 38 131 35 649.0 0 35 132 23 649.0 0 23 133 51 649.0 0 51 134 34 649.0 0 34 135 43 649.0 0 43 136 60 649.0 0 60 137 68 649.0 0 68 138 44 649.0 0 44 139 63 649.0 0 63 140 57 649.0 0 57 141 107 649.0 0 107 142 38 649.0 0 38 143 39 649.0 0 39 144 42 649.0 0 42 145 84 649.0 0 84 146 35 649.0 0 35 147 81 649.0 0 81 148 39 649.0 0 39 149 117 649.0 0 117 150 47 649.0 0 47 151 45 649.0 0 45 152 40 649.0 0 40 153 68 649.0 0 68 154 62 649.0 0 62 155 30 649.0 0 30 156 37 649.0 0 37 157 43 649.0 0 43 158 41 649.0 0 41 159 56 649.0 0 56 160 193 649.0 0 193 161 167 649.0 0 167 162 90 649.0 0 90 163 41 649.0 0 41 164 29 649.0 0 29 165 48 649.0 0 48 166 66 649.0 0 66 167 51 649.0 0 51 168 43 649.0 0 43 169 31 649.0 0 31 170 26 649.0 0 26 171 23 649.0 0 23 172 32 649.0 0 32 173 46 649.0 0 46 174 41 649.0 0 41 175 93 649.0 0 93 176 122 649.0 0 122 177 99 649.0 0 99 178 74 649.0 0 74 179 36 649.0 0 36 180 54 649.0 0 54 181 112 649.0 0 112 182 67 649.0 0 67 183 31 649.0 0 31 184 37 649.0 0 37 185 39 649.0 0 39 186 54 649.0 0 54 187 32 649.0 0 32 188 56 649.0 0 56 189 39 649.0 0 39 190 30 649.0 0 30 191 36 649.0 0 36 192 36 649.0 0 36 193 58 649.0 0 58 194 59 649.0 0 59 195 35 649.0 0 35 196 43 649.0 0 43 197 36 649.0 0 36 198 30 649.0 0 30 199 32 649.0 0 32 200 42 649.0 0 42 201 24 649.0 0 24 202 35 649.0 0 35 203 46 649.0 0 46 204 174 649.0 0 174 205 42 649.0 0 42 206 31 649.0 0 31 207 32 649.0 0 32 208 37 649.0 0 37 209 36 649.0 0 36 210 36 649.0 0 36 211 36 649.0 0 36 212 32 649.0 0 32 213 48 649.0 0 48 214 49 649.0 0 49 215 38 649.0 0 38 216 37 649.0 0 37 217 32 649.0 0 32 218 33 649.0 0 33 219 48 649.0 0 48 220 49 649.0 0 49 221 52 649.0 0 52 222 40 649.0 0 40 223 39 649.0 0 39 224 40 649.0 0 40 225 35 649.0 0 35 226 48 649.0 0 48 227 51 649.0 0 51 228 53 649.0 0 53 229 53 649.0 0 53 230 42 649.0 0 42 231 47 649.0 0 47 232 46 649.0 0 46 233 35 649.0 0 35 234 41 649.0 0 41 235 31 649.0 0 31 236 48 649.0 0 48 237 70 649.0 0 70 238 56 649.0 0 56 239 68 649.0 0 68 240 40 649.0 0 40 241 35 649.0 0 35 242 48 649.0 0 48 243 40 649.0 0 40 244 36 649.0 0 36 245 30 649.0 0 30 246 34 649.0 0 34 247 44 649.0 0 44 248 50 649.0 0 50 249 48 649.0 0 48 250 45 649.0 0 45 251 33 649.0 0 33 252 51 649.0 0 51 253 41 649.0 0 41 254 56 649.0 0 56 255 69 649.0 0 69 256 58 649.0 0 58 257 66 649.0 0 66 258 42 649.0 0 42 259 39 649.0 0 39 260 44 649.0 0 44 261 60 649.0 0 60 262 125 649.0 0 125 263 106 649.0 0 106 264 104 649.0 0 104 265 44 649.0 0 44 266 36 649.0 0 36 267 49 649.0 0 49 268 43 649.0 0 43 269 35 649.0 0 35 270 54 649.0 0 54 271 39 649.0 0 39 272 32 649.0 0 32 273 82 649.0 0 82 274 79 649.0 0 79 275 75 649.0 0 75 276 51 649.0 0 51 277 33 649.0 0 33 278 67 649.0 0 67 279 71 649.0 0 71 280 104 649.0 0 104 281 55 649.0 0 55 282 65 649.0 0 65 283 95 649.0 0 95 284 73 649.0 0 73 285 43 649.0 0 43 286 27 649.0 0 27 287 45 649.0 0 45 288 54 649.0 0 54 289 48 649.0 0 48 290 51 649.0 0 51 291 96 649.0 0 96 292 92 649.0 0 92 293 65 649.0 0 65 294 59 649.0 0 59 295 45 649.0 0 45 296 59 649.0 0 59 297 49 649.0 0 49 298 42 649.0 0 42 299 61 649.0 0 61 300 90 649.0 0 90 301 63 649.0 0 63 Finished in 2.62 s (51 us/read; 1.19 M reads/minute). === Summary === Total reads processed: 51,794 Reads with adapters: 10,353 (20.0%) Reads that were too short: 11 (0.0%) Reads written (passing filters): 10,342 (20.0%) Total basepairs processed: 13,007,460 bp Total written (filtered): 2,815,884 bp (21.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 10353 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 1 0.0 3 0 0 1 19 120 0.0 3 0 117 1 2 20 10023 0.0 4 9850 162 11 21 24 0.0 4 5 19 22 5 0.0 4 4 0 0 0 1 23 7 0.0 4 0 0 0 6 1 35 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 77 2 0.0 4 0 0 0 1 1 101 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 67 0.0 4 0 0 0 0 67 129 33 0.0 4 0 0 0 0 33 130 2 0.0 4 0 0 0 0 2 132 1 0.0 4 0 0 0 0 1 135 53 0.0 4 0 0 0 0 53 148 2 0.0 4 0 0 0 0 2 162 1 0.0 4 0 0 0 0 1 171 1 0.0 4 0 0 0 0 1 179 1 0.0 4 1 228 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.35 s (34 us/read; 1.77 M reads/minute). === Summary === Total reads processed: 10,342 Reads with adapters: 285 (2.8%) Reads written (passing filters): 10,342 (100.0%) Total basepairs processed: 2,815,884 bp Total written (filtered): 2,811,807 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 285 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 8.4% C: 7.4% G: 82.8% T: 1.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 21 161.6 0 21 4 3 40.4 0 3 9 1 0.0 0 0 1 10 7 0.0 1 0 7 11 225 0.0 1 1 224 12 14 0.0 1 0 14 13 1 0.0 1 0 1 20 6 0.0 1 6 94 1 0.0 1 1 113 1 0.0 1 1 139 1 0.0 1 1 143 1 0.0 1 1 174 1 0.0 1 1 225 1 0.0 1 1 259 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.