This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATGAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.84 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 138,625 (5.2%) Reads that were too short: 19,550 (0.7%) Reads written (passing filters): 119,075 (4.5%) Total basepairs processed: 800,111,879 bp Total written (filtered): 30,322,754 bp (3.8%) === Adapter 1 === Sequence: AATGAA; Type: regular 5'; Length: 6; Trimmed: 138625 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 679 649.0 0 679 7 1179 649.0 0 1179 8 41028 649.0 0 41028 9 35164 649.0 0 35164 10 75 649.0 0 75 11 49 649.0 0 49 12 32 649.0 0 32 13 61 649.0 0 61 14 29 649.0 0 29 15 29 649.0 0 29 16 27 649.0 0 27 17 49 649.0 0 49 18 38 649.0 0 38 19 24 649.0 0 24 20 35 649.0 0 35 21 36 649.0 0 36 22 43 649.0 0 43 23 40 649.0 0 40 24 37 649.0 0 37 25 38 649.0 0 38 26 34 649.0 0 34 27 39 649.0 0 39 28 57 649.0 0 57 29 35 649.0 0 35 30 30 649.0 0 30 31 63 649.0 0 63 32 80 649.0 0 80 33 39 649.0 0 39 34 32 649.0 0 32 35 47 649.0 0 47 36 120 649.0 0 120 37 26 649.0 0 26 38 44 649.0 0 44 39 50 649.0 0 50 40 50 649.0 0 50 41 97 649.0 0 97 42 158 649.0 0 158 43 72 649.0 0 72 44 107 649.0 0 107 45 139 649.0 0 139 46 63 649.0 0 63 47 130 649.0 0 130 48 37 649.0 0 37 49 48 649.0 0 48 50 83 649.0 0 83 51 54 649.0 0 54 52 38 649.0 0 38 53 34 649.0 0 34 54 24 649.0 0 24 55 34 649.0 0 34 56 35 649.0 0 35 57 61 649.0 0 61 58 53 649.0 0 53 59 55 649.0 0 55 60 49 649.0 0 49 61 38 649.0 0 38 62 60 649.0 0 60 63 43 649.0 0 43 64 43 649.0 0 43 65 154 649.0 0 154 66 105 649.0 0 105 67 52 649.0 0 52 68 91 649.0 0 91 69 72 649.0 0 72 70 113 649.0 0 113 71 124 649.0 0 124 72 192 649.0 0 192 73 190 649.0 0 190 74 979 649.0 0 979 75 438 649.0 0 438 76 431 649.0 0 431 77 455 649.0 0 455 78 279 649.0 0 279 79 2166 649.0 0 2166 80 1745 649.0 0 1745 81 2407 649.0 0 2407 82 320 649.0 0 320 83 337 649.0 0 337 84 439 649.0 0 439 85 82 649.0 0 82 86 128 649.0 0 128 87 105 649.0 0 105 88 88 649.0 0 88 89 63 649.0 0 63 90 82 649.0 0 82 91 51 649.0 0 51 92 68 649.0 0 68 93 88 649.0 0 88 94 91 649.0 0 91 95 85 649.0 0 85 96 91 649.0 0 91 97 214 649.0 0 214 98 131 649.0 0 131 99 148 649.0 0 148 100 374 649.0 0 374 101 306 649.0 0 306 102 97 649.0 0 97 103 141 649.0 0 141 104 190 649.0 0 190 105 165 649.0 0 165 106 151 649.0 0 151 107 193 649.0 0 193 108 192 649.0 0 192 109 156 649.0 0 156 110 139 649.0 0 139 111 188 649.0 0 188 112 256 649.0 0 256 113 219 649.0 0 219 114 236 649.0 0 236 115 284 649.0 0 284 116 275 649.0 0 275 117 245 649.0 0 245 118 236 649.0 0 236 119 224 649.0 0 224 120 243 649.0 0 243 121 255 649.0 0 255 122 215 649.0 0 215 123 271 649.0 0 271 124 415 649.0 0 415 125 1115 649.0 0 1115 126 560 649.0 0 560 127 945 649.0 0 945 128 257 649.0 0 257 129 384 649.0 0 384 130 295 649.0 0 295 131 187 649.0 0 187 132 186 649.0 0 186 133 142 649.0 0 142 134 188 649.0 0 188 135 185 649.0 0 185 136 264 649.0 0 264 137 442 649.0 0 442 138 474 649.0 0 474 139 469 649.0 0 469 140 275 649.0 0 275 141 697 649.0 0 697 142 402 649.0 0 402 143 835 649.0 0 835 144 151 649.0 0 151 145 169 649.0 0 169 146 158 649.0 0 158 147 178 649.0 0 178 148 144 649.0 0 144 149 158 649.0 0 158 150 210 649.0 0 210 151 152 649.0 0 152 152 193 649.0 0 193 153 111 649.0 0 111 154 128 649.0 0 128 155 120 649.0 0 120 156 144 649.0 0 144 157 162 649.0 0 162 158 475 649.0 0 475 159 323 649.0 0 323 160 346 649.0 0 346 161 157 649.0 0 157 162 114 649.0 0 114 163 157 649.0 0 157 164 186 649.0 0 186 165 159 649.0 0 159 166 155 649.0 0 155 167 118 649.0 0 118 168 120 649.0 0 120 169 144 649.0 0 144 170 131 649.0 0 131 171 126 649.0 0 126 172 133 649.0 0 133 173 112 649.0 0 112 174 117 649.0 0 117 175 101 649.0 0 101 176 108 649.0 0 108 177 144 649.0 0 144 178 164 649.0 0 164 179 140 649.0 0 140 180 131 649.0 0 131 181 210 649.0 0 210 182 1301 649.0 0 1301 183 1137 649.0 0 1137 184 846 649.0 0 846 185 157 649.0 0 157 186 100 649.0 0 100 187 121 649.0 0 121 188 121 649.0 0 121 189 131 649.0 0 131 190 102 649.0 0 102 191 104 649.0 0 104 192 97 649.0 0 97 193 114 649.0 0 114 194 107 649.0 0 107 195 113 649.0 0 113 196 119 649.0 0 119 197 112 649.0 0 112 198 131 649.0 0 131 199 101 649.0 0 101 200 133 649.0 0 133 201 114 649.0 0 114 202 120 649.0 0 120 203 133 649.0 0 133 204 136 649.0 0 136 205 142 649.0 0 142 206 182 649.0 0 182 207 145 649.0 0 145 208 192 649.0 0 192 209 152 649.0 0 152 210 150 649.0 0 150 211 178 649.0 0 178 212 157 649.0 0 157 213 101 649.0 0 101 214 126 649.0 0 126 215 114 649.0 0 114 216 119 649.0 0 119 217 101 649.0 0 101 218 130 649.0 0 130 219 119 649.0 0 119 220 133 649.0 0 133 221 132 649.0 0 132 222 113 649.0 0 113 223 124 649.0 0 124 224 142 649.0 0 142 225 173 649.0 0 173 226 191 649.0 0 191 227 195 649.0 0 195 228 120 649.0 0 120 229 105 649.0 0 105 230 102 649.0 0 102 231 171 649.0 0 171 232 149 649.0 0 149 233 129 649.0 0 129 234 116 649.0 0 116 235 127 649.0 0 127 236 151 649.0 0 151 237 404 649.0 0 404 238 480 649.0 0 480 239 325 649.0 0 325 240 288 649.0 0 288 241 143 649.0 0 143 242 140 649.0 0 140 243 101 649.0 0 101 244 97 649.0 0 97 245 112 649.0 0 112 246 126 649.0 0 126 247 106 649.0 0 106 248 126 649.0 0 126 249 153 649.0 0 153 250 158 649.0 0 158 251 131 649.0 0 131 252 189 649.0 0 189 253 1082 649.0 0 1082 254 1387 649.0 0 1387 255 849 649.0 0 849 256 585 649.0 0 585 257 156 649.0 0 156 258 122 649.0 0 122 259 149 649.0 0 149 260 135 649.0 0 135 261 146 649.0 0 146 262 131 649.0 0 131 263 151 649.0 0 151 264 385 649.0 0 385 265 251 649.0 0 251 266 371 649.0 0 371 267 83 649.0 0 83 268 94 649.0 0 94 269 133 649.0 0 133 270 114 649.0 0 114 271 120 649.0 0 120 272 137 649.0 0 137 273 145 649.0 0 145 274 146 649.0 0 146 275 215 649.0 0 215 276 175 649.0 0 175 277 224 649.0 0 224 278 199 649.0 0 199 279 139 649.0 0 139 280 138 649.0 0 138 281 153 649.0 0 153 282 205 649.0 0 205 283 150 649.0 0 150 284 146 649.0 0 146 285 125 649.0 0 125 286 147 649.0 0 147 287 113 649.0 0 113 288 236 649.0 0 236 289 284 649.0 0 284 290 332 649.0 0 332 291 203 649.0 0 203 292 98 649.0 0 98 293 133 649.0 0 133 294 107 649.0 0 107 295 128 649.0 0 128 296 163 649.0 0 163 297 186 649.0 0 186 298 163 649.0 0 163 299 171 649.0 0 171 300 137 649.0 0 137 301 259 649.0 0 259 Finished in 5.50 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 119,075 Reads with adapters: 41,250 (34.6%) Reads that were too short: 214 (0.2%) Reads written (passing filters): 41,036 (34.5%) Total basepairs processed: 30,322,754 bp Total written (filtered): 11,278,155 bp (37.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 41250 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 5 0.0 2 0 0 0 5 15 27 0.0 3 0 0 17 10 16 395 0.0 3 0 345 30 20 17 40004 0.0 3 37950 1741 257 56 18 123 0.0 3 9 89 10 15 19 81 0.0 3 1 1 46 33 20 91 0.0 3 1 0 0 90 21 6 0.0 3 2 0 0 4 22 2 0.0 3 0 0 2 24 3 0.0 3 1 0 0 2 25 7 0.0 3 0 0 0 7 28 9 0.0 3 0 0 0 9 31 4 0.0 3 0 0 0 4 34 2 0.0 3 0 0 0 2 35 2 0.0 3 0 0 0 2 39 1 0.0 3 0 0 0 1 40 2 0.0 3 0 0 0 2 41 29 0.0 3 0 0 0 29 44 12 0.0 3 0 0 0 12 46 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 51 16 0.0 3 0 0 0 16 52 11 0.0 3 0 0 0 11 53 1 0.0 3 0 0 0 1 54 4 0.0 3 0 0 0 4 55 1 0.0 3 0 0 0 1 57 2 0.0 3 0 0 0 2 58 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 63 2 0.0 3 0 0 0 2 64 1 0.0 3 0 0 0 1 65 84 0.0 3 0 0 1 83 67 20 0.0 3 0 0 0 20 68 3 0.0 3 0 0 0 3 69 1 0.0 3 0 0 0 1 72 2 0.0 3 0 0 0 2 76 1 0.0 3 0 0 0 1 77 1 0.0 3 1 78 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 81 2 0.0 3 0 0 0 2 83 1 0.0 3 0 0 0 1 84 3 0.0 3 0 0 1 2 86 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 90 5 0.0 3 1 0 0 4 91 1 0.0 3 0 0 0 1 92 3 0.0 3 0 0 0 3 93 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 100 4 0.0 3 0 0 1 3 101 5 0.0 3 0 0 1 4 102 1 0.0 3 0 0 0 1 103 2 0.0 3 0 0 0 2 104 2 0.0 3 0 0 0 2 105 1 0.0 3 0 0 0 1 106 2 0.0 3 0 0 0 2 107 3 0.0 3 0 0 0 3 109 5 0.0 3 0 0 0 5 110 1 0.0 3 0 0 0 1 112 15 0.0 3 0 0 2 13 113 6 0.0 3 0 0 0 6 114 1 0.0 3 0 0 0 1 115 2 0.0 3 0 0 0 2 117 2 0.0 3 0 0 0 2 118 2 0.0 3 0 0 0 2 119 2 0.0 3 0 0 0 2 120 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 123 6 0.0 3 0 0 0 6 124 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 126 2 0.0 3 0 0 0 2 127 2 0.0 3 0 0 0 2 128 1 0.0 3 0 0 0 1 129 4 0.0 3 0 0 0 4 130 3 0.0 3 0 0 0 3 132 2 0.0 3 0 0 0 2 133 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 136 10 0.0 3 0 0 0 10 138 1 0.0 3 0 0 0 1 143 2 0.0 3 0 0 0 2 144 2 0.0 3 0 0 0 2 145 14 0.0 3 0 0 0 14 146 2 0.0 3 0 0 0 2 147 5 0.0 3 0 0 1 4 150 1 0.0 3 0 0 0 1 154 2 0.0 3 0 0 0 2 155 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 172 12 0.0 3 0 0 0 12 173 2 0.0 3 0 0 0 2 175 4 0.0 3 0 0 0 4 179 1 0.0 3 0 0 0 1 182 2 0.0 3 0 0 0 2 183 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 190 2 0.0 3 0 0 0 2 191 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 194 6 0.0 3 0 0 0 6 195 2 0.0 3 0 0 0 2 203 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 205 3 0.0 3 0 0 0 3 206 3 0.0 3 0 0 0 3 210 1 0.0 3 0 0 0 1 213 5 0.0 3 0 0 0 5 214 8 0.0 3 0 0 1 7 215 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 39 0.0 3 0 0 5 34 218 3 0.0 3 0 0 0 3 221 1 0.0 3 0 0 0 1 226 1 0.0 3 0 0 1 229 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 238 2 0.0 3 0 0 0 2 244 1 0.0 3 0 0 0 1 256 1 0.0 3 0 0 0 1 266 1 0.0 3 0 0 0 1 268 1 0.0 3 0 0 0 1 270 2 0.0 3 0 0 0 2 278 2 0.0 3 0 0 0 2 279 2 0.0 3 0 0 0 2 280 7 0.0 3 0 0 0 7 281 1 0.0 3 0 0 0 1 282 4 0.0 3 0 0 0 4 284 1 0.0 3 0 0 0 1 287 2 0.0 3 0 0 0 2 289 1 0.0 3 0 0 0 1 292 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.44 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 41,036 Reads with adapters: 845 (2.1%) Reads written (passing filters): 41,036 (100.0%) Total basepairs processed: 11,278,155 bp Total written (filtered): 11,275,510 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 845 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 41.3% C: 12.1% G: 41.7% T: 5.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 753 641.2 0 753 4 80 160.3 0 80 5 11 40.1 0 11 11 1 0.0 1 0 1