This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATGAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 69.63 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 149,277 (5.6%) Reads that were too short: 23,706 (0.9%) Reads written (passing filters): 125,571 (4.7%) Total basepairs processed: 800,111,879 bp Total written (filtered): 31,673,063 bp (4.0%) === Adapter 1 === Sequence: AATGAA; Type: regular 5'; Length: 6; Trimmed: 149277 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 473 649.0 0 473 7 767 649.0 0 767 8 38111 649.0 0 38111 9 40772 649.0 0 40772 10 84 649.0 0 84 11 64 649.0 0 64 12 51 649.0 0 51 13 57 649.0 0 57 14 45 649.0 0 45 15 47 649.0 0 47 16 47 649.0 0 47 17 54 649.0 0 54 18 46 649.0 0 46 19 57 649.0 0 57 20 45 649.0 0 45 21 56 649.0 0 56 22 59 649.0 0 59 23 74 649.0 0 74 24 47 649.0 0 47 25 66 649.0 0 66 26 78 649.0 0 78 27 62 649.0 0 62 28 70 649.0 0 70 29 64 649.0 0 64 30 55 649.0 0 55 31 64 649.0 0 64 32 71 649.0 0 71 33 35 649.0 0 35 34 48 649.0 0 48 35 71 649.0 0 71 36 177 649.0 0 177 37 59 649.0 0 59 38 194 649.0 0 194 39 65 649.0 0 65 40 69 649.0 0 69 41 109 649.0 0 109 42 160 649.0 0 160 43 98 649.0 0 98 44 160 649.0 0 160 45 133 649.0 0 133 46 84 649.0 0 84 47 100 649.0 0 100 48 66 649.0 0 66 49 65 649.0 0 65 50 102 649.0 0 102 51 76 649.0 0 76 52 50 649.0 0 50 53 40 649.0 0 40 54 51 649.0 0 51 55 44 649.0 0 44 56 58 649.0 0 58 57 112 649.0 0 112 58 83 649.0 0 83 59 62 649.0 0 62 60 69 649.0 0 69 61 48 649.0 0 48 62 65 649.0 0 65 63 93 649.0 0 93 64 67 649.0 0 67 65 200 649.0 0 200 66 157 649.0 0 157 67 87 649.0 0 87 68 114 649.0 0 114 69 92 649.0 0 92 70 126 649.0 0 126 71 112 649.0 0 112 72 105 649.0 0 105 73 89 649.0 0 89 74 112 649.0 0 112 75 86 649.0 0 86 76 109 649.0 0 109 77 152 649.0 0 152 78 194 649.0 0 194 79 2352 649.0 0 2352 80 1951 649.0 0 1951 81 2567 649.0 0 2567 82 391 649.0 0 391 83 347 649.0 0 347 84 638 649.0 0 638 85 209 649.0 0 209 86 198 649.0 0 198 87 171 649.0 0 171 88 331 649.0 0 331 89 166 649.0 0 166 90 206 649.0 0 206 91 243 649.0 0 243 92 213 649.0 0 213 93 174 649.0 0 174 94 170 649.0 0 170 95 213 649.0 0 213 96 191 649.0 0 191 97 233 649.0 0 233 98 275 649.0 0 275 99 240 649.0 0 240 100 191 649.0 0 191 101 206 649.0 0 206 102 253 649.0 0 253 103 219 649.0 0 219 104 203 649.0 0 203 105 169 649.0 0 169 106 176 649.0 0 176 107 223 649.0 0 223 108 283 649.0 0 283 109 222 649.0 0 222 110 227 649.0 0 227 111 197 649.0 0 197 112 212 649.0 0 212 113 190 649.0 0 190 114 251 649.0 0 251 115 286 649.0 0 286 116 285 649.0 0 285 117 315 649.0 0 315 118 269 649.0 0 269 119 236 649.0 0 236 120 274 649.0 0 274 121 261 649.0 0 261 122 223 649.0 0 223 123 265 649.0 0 265 124 375 649.0 0 375 125 731 649.0 0 731 126 414 649.0 0 414 127 709 649.0 0 709 128 287 649.0 0 287 129 376 649.0 0 376 130 316 649.0 0 316 131 252 649.0 0 252 132 216 649.0 0 216 133 179 649.0 0 179 134 218 649.0 0 218 135 224 649.0 0 224 136 331 649.0 0 331 137 464 649.0 0 464 138 542 649.0 0 542 139 443 649.0 0 443 140 283 649.0 0 283 141 694 649.0 0 694 142 515 649.0 0 515 143 912 649.0 0 912 144 216 649.0 0 216 145 176 649.0 0 176 146 171 649.0 0 171 147 181 649.0 0 181 148 183 649.0 0 183 149 183 649.0 0 183 150 245 649.0 0 245 151 227 649.0 0 227 152 212 649.0 0 212 153 179 649.0 0 179 154 169 649.0 0 169 155 176 649.0 0 176 156 207 649.0 0 207 157 179 649.0 0 179 158 566 649.0 0 566 159 406 649.0 0 406 160 424 649.0 0 424 161 175 649.0 0 175 162 182 649.0 0 182 163 155 649.0 0 155 164 277 649.0 0 277 165 200 649.0 0 200 166 179 649.0 0 179 167 165 649.0 0 165 168 183 649.0 0 183 169 158 649.0 0 158 170 203 649.0 0 203 171 204 649.0 0 204 172 186 649.0 0 186 173 151 649.0 0 151 174 135 649.0 0 135 175 184 649.0 0 184 176 150 649.0 0 150 177 198 649.0 0 198 178 197 649.0 0 197 179 190 649.0 0 190 180 173 649.0 0 173 181 255 649.0 0 255 182 1057 649.0 0 1057 183 1173 649.0 0 1173 184 870 649.0 0 870 185 187 649.0 0 187 186 132 649.0 0 132 187 166 649.0 0 166 188 181 649.0 0 181 189 171 649.0 0 171 190 152 649.0 0 152 191 191 649.0 0 191 192 136 649.0 0 136 193 145 649.0 0 145 194 156 649.0 0 156 195 188 649.0 0 188 196 169 649.0 0 169 197 200 649.0 0 200 198 188 649.0 0 188 199 184 649.0 0 184 200 204 649.0 0 204 201 191 649.0 0 191 202 173 649.0 0 173 203 198 649.0 0 198 204 176 649.0 0 176 205 197 649.0 0 197 206 203 649.0 0 203 207 213 649.0 0 213 208 213 649.0 0 213 209 202 649.0 0 202 210 191 649.0 0 191 211 201 649.0 0 201 212 211 649.0 0 211 213 153 649.0 0 153 214 153 649.0 0 153 215 186 649.0 0 186 216 151 649.0 0 151 217 178 649.0 0 178 218 151 649.0 0 151 219 168 649.0 0 168 220 140 649.0 0 140 221 189 649.0 0 189 222 167 649.0 0 167 223 174 649.0 0 174 224 205 649.0 0 205 225 204 649.0 0 204 226 241 649.0 0 241 227 221 649.0 0 221 228 166 649.0 0 166 229 152 649.0 0 152 230 141 649.0 0 141 231 204 649.0 0 204 232 146 649.0 0 146 233 154 649.0 0 154 234 121 649.0 0 121 235 173 649.0 0 173 236 199 649.0 0 199 237 333 649.0 0 333 238 369 649.0 0 369 239 256 649.0 0 256 240 228 649.0 0 228 241 175 649.0 0 175 242 170 649.0 0 170 243 165 649.0 0 165 244 125 649.0 0 125 245 157 649.0 0 157 246 163 649.0 0 163 247 162 649.0 0 162 248 169 649.0 0 169 249 177 649.0 0 177 250 211 649.0 0 211 251 207 649.0 0 207 252 253 649.0 0 253 253 1631 649.0 0 1631 254 2547 649.0 0 2547 255 1325 649.0 0 1325 256 1158 649.0 0 1158 257 199 649.0 0 199 258 117 649.0 0 117 259 168 649.0 0 168 260 146 649.0 0 146 261 166 649.0 0 166 262 160 649.0 0 160 263 162 649.0 0 162 264 231 649.0 0 231 265 199 649.0 0 199 266 239 649.0 0 239 267 99 649.0 0 99 268 142 649.0 0 142 269 159 649.0 0 159 270 122 649.0 0 122 271 137 649.0 0 137 272 141 649.0 0 141 273 149 649.0 0 149 274 188 649.0 0 188 275 191 649.0 0 191 276 172 649.0 0 172 277 215 649.0 0 215 278 157 649.0 0 157 279 155 649.0 0 155 280 151 649.0 0 151 281 151 649.0 0 151 282 189 649.0 0 189 283 125 649.0 0 125 284 174 649.0 0 174 285 134 649.0 0 134 286 138 649.0 0 138 287 163 649.0 0 163 288 162 649.0 0 162 289 216 649.0 0 216 290 253 649.0 0 253 291 180 649.0 0 180 292 129 649.0 0 129 293 117 649.0 0 117 294 151 649.0 0 151 295 123 649.0 0 123 296 158 649.0 0 158 297 169 649.0 0 169 298 152 649.0 0 152 299 170 649.0 0 170 300 150 649.0 0 150 301 241 649.0 0 241 Finished in 6.25 s (50 us/read; 1.21 M reads/minute). === Summary === Total reads processed: 125,571 Reads with adapters: 42,637 (34.0%) Reads that were too short: 40 (0.0%) Reads written (passing filters): 42,597 (33.9%) Total basepairs processed: 31,673,063 bp Total written (filtered): 11,553,390 bp (36.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 42637 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 4 0.0 3 0 0 0 0 4 18 13 0.0 3 0 0 13 19 526 0.0 3 0 517 6 1 2 20 41586 0.0 4 40731 806 41 7 1 21 92 0.0 4 20 69 3 22 4 0.0 4 2 0 2 23 37 0.0 4 1 1 0 31 4 24 5 0.0 4 2 0 0 0 3 27 1 0.0 4 0 0 0 0 1 30 1 0.0 4 0 0 0 0 1 33 1 0.0 4 0 0 0 0 1 39 1 0.0 4 1 45 1 0.0 4 0 0 0 0 1 49 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 57 2 0.0 4 0 0 0 0 2 61 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 0 1 77 2 0.0 4 0 0 0 1 1 78 1 0.0 4 0 0 0 1 81 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 1 95 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 111 2 0.0 4 0 0 0 0 2 115 1 0.0 4 0 0 0 0 1 117 2 0.0 4 0 0 0 0 2 119 1 0.0 4 0 0 0 0 1 124 1 0.0 4 1 125 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 302 0.0 4 0 0 0 0 302 129 1 0.0 4 0 0 0 0 1 130 4 0.0 4 0 0 0 0 4 132 1 0.0 4 0 0 0 0 1 135 3 0.0 4 0 0 0 0 3 147 2 0.0 4 0 0 0 0 2 153 2 0.0 4 0 0 0 0 2 158 1 0.0 4 0 1 161 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 0 0 0 1 163 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 184 1 0.0 4 0 0 0 1 193 1 0.0 4 0 0 0 0 1 194 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 223 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 0 3 229 1 0.0 4 0 0 0 0 1 231 1 0.0 4 0 0 0 0 1 241 1 0.0 4 1 263 1 0.0 4 0 0 0 0 1 289 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.55 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 42,597 Reads with adapters: 7,932 (18.6%) Reads written (passing filters): 42,597 (100.0%) Total basepairs processed: 11,553,390 bp Total written (filtered): 11,463,632 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 7932 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.0% C: 3.3% G: 94.6% T: 1.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 10 665.6 0 10 4 3 166.4 0 3 5 1 41.6 0 1 6 1 10.4 0 1 8 3 0.6 0 3 9 5320 0.2 0 162 5158 10 1995 0.0 1 65 1930 11 506 0.0 1 3 503 12 17 0.0 1 0 17 13 1 0.0 1 0 1 14 1 0.0 1 0 1 80 1 0.0 1 1 120 1 0.0 1 1 150 2 0.0 1 0 2 208 4 0.0 1 2 2 222 1 0.0 1 1 223 60 0.0 1 58 2 224 5 0.0 1 4 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.