This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTATAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 70.66 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 60,472 (2.3%) Reads that were too short: 16,162 (0.6%) Reads written (passing filters): 44,310 (1.7%) Total basepairs processed: 800,111,879 bp Total written (filtered): 10,749,350 bp (1.3%) === Adapter 1 === Sequence: CTATAA; Type: regular 5'; Length: 6; Trimmed: 60472 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 457 649.0 0 457 7 13090 649.0 0 13090 8 11462 649.0 0 11462 9 45 649.0 0 45 10 38 649.0 0 38 11 36 649.0 0 36 12 22 649.0 0 22 13 37 649.0 0 37 14 45 649.0 0 45 15 47 649.0 0 47 16 53 649.0 0 53 17 39 649.0 0 39 18 37 649.0 0 37 19 44 649.0 0 44 20 28 649.0 0 28 21 25 649.0 0 25 22 26 649.0 0 26 23 35 649.0 0 35 24 36 649.0 0 36 25 42 649.0 0 42 26 115 649.0 0 115 27 95 649.0 0 95 28 86 649.0 0 86 29 30 649.0 0 30 30 28 649.0 0 28 31 32 649.0 0 32 32 42 649.0 0 42 33 43 649.0 0 43 34 55 649.0 0 55 35 13 649.0 0 13 36 57 649.0 0 57 37 101 649.0 0 101 38 159 649.0 0 159 39 38 649.0 0 38 40 32 649.0 0 32 41 30 649.0 0 30 42 45 649.0 0 45 43 26 649.0 0 26 44 27 649.0 0 27 45 32 649.0 0 32 46 28 649.0 0 28 47 28 649.0 0 28 48 17 649.0 0 17 49 38 649.0 0 38 50 31 649.0 0 31 51 34 649.0 0 34 52 25 649.0 0 25 53 25 649.0 0 25 54 43 649.0 0 43 55 23 649.0 0 23 56 32 649.0 0 32 57 18 649.0 0 18 58 40 649.0 0 40 59 54 649.0 0 54 60 28 649.0 0 28 61 122 649.0 0 122 62 24 649.0 0 24 63 29 649.0 0 29 64 26 649.0 0 26 65 27 649.0 0 27 66 27 649.0 0 27 67 29 649.0 0 29 68 18 649.0 0 18 69 39 649.0 0 39 70 27 649.0 0 27 71 34 649.0 0 34 72 26 649.0 0 26 73 19 649.0 0 19 74 32 649.0 0 32 75 160 649.0 0 160 76 81 649.0 0 81 77 145 649.0 0 145 78 57 649.0 0 57 79 76 649.0 0 76 80 50 649.0 0 50 81 76 649.0 0 76 82 45 649.0 0 45 83 55 649.0 0 55 84 57 649.0 0 57 85 50 649.0 0 50 86 42 649.0 0 42 87 40 649.0 0 40 88 42 649.0 0 42 89 31 649.0 0 31 90 35 649.0 0 35 91 43 649.0 0 43 92 45 649.0 0 45 93 53 649.0 0 53 94 87 649.0 0 87 95 52 649.0 0 52 96 167 649.0 0 167 97 111 649.0 0 111 98 95 649.0 0 95 99 200 649.0 0 200 100 344 649.0 0 344 101 169 649.0 0 169 102 152 649.0 0 152 103 125 649.0 0 125 104 108 649.0 0 108 105 150 649.0 0 150 106 185 649.0 0 185 107 153 649.0 0 153 108 586 649.0 0 586 109 300 649.0 0 300 110 457 649.0 0 457 111 178 649.0 0 178 112 180 649.0 0 180 113 138 649.0 0 138 114 219 649.0 0 219 115 198 649.0 0 198 116 229 649.0 0 229 117 128 649.0 0 128 118 89 649.0 0 89 119 86 649.0 0 86 120 67 649.0 0 67 121 91 649.0 0 91 122 155 649.0 0 155 123 147 649.0 0 147 124 81 649.0 0 81 125 91 649.0 0 91 126 109 649.0 0 109 127 111 649.0 0 111 128 134 649.0 0 134 129 176 649.0 0 176 130 243 649.0 0 243 131 220 649.0 0 220 132 202 649.0 0 202 133 109 649.0 0 109 134 126 649.0 0 126 135 149 649.0 0 149 136 192 649.0 0 192 137 241 649.0 0 241 138 118 649.0 0 118 139 129 649.0 0 129 140 107 649.0 0 107 141 130 649.0 0 130 142 158 649.0 0 158 143 120 649.0 0 120 144 151 649.0 0 151 145 127 649.0 0 127 146 110 649.0 0 110 147 124 649.0 0 124 148 128 649.0 0 128 149 121 649.0 0 121 150 108 649.0 0 108 151 96 649.0 0 96 152 97 649.0 0 97 153 104 649.0 0 104 154 113 649.0 0 113 155 156 649.0 0 156 156 167 649.0 0 167 157 148 649.0 0 148 158 156 649.0 0 156 159 123 649.0 0 123 160 101 649.0 0 101 161 123 649.0 0 123 162 260 649.0 0 260 163 141 649.0 0 141 164 328 649.0 0 328 165 144 649.0 0 144 166 117 649.0 0 117 167 148 649.0 0 148 168 116 649.0 0 116 169 142 649.0 0 142 170 129 649.0 0 129 171 127 649.0 0 127 172 102 649.0 0 102 173 93 649.0 0 93 174 80 649.0 0 80 175 94 649.0 0 94 176 102 649.0 0 102 177 96 649.0 0 96 178 127 649.0 0 127 179 119 649.0 0 119 180 111 649.0 0 111 181 97 649.0 0 97 182 89 649.0 0 89 183 114 649.0 0 114 184 112 649.0 0 112 185 103 649.0 0 103 186 104 649.0 0 104 187 107 649.0 0 107 188 115 649.0 0 115 189 107 649.0 0 107 190 154 649.0 0 154 191 109 649.0 0 109 192 114 649.0 0 114 193 100 649.0 0 100 194 95 649.0 0 95 195 100 649.0 0 100 196 105 649.0 0 105 197 97 649.0 0 97 198 86 649.0 0 86 199 111 649.0 0 111 200 149 649.0 0 149 201 165 649.0 0 165 202 193 649.0 0 193 203 166 649.0 0 166 204 157 649.0 0 157 205 115 649.0 0 115 206 114 649.0 0 114 207 134 649.0 0 134 208 206 649.0 0 206 209 207 649.0 0 207 210 208 649.0 0 208 211 127 649.0 0 127 212 85 649.0 0 85 213 104 649.0 0 104 214 81 649.0 0 81 215 105 649.0 0 105 216 123 649.0 0 123 217 101 649.0 0 101 218 88 649.0 0 88 219 83 649.0 0 83 220 113 649.0 0 113 221 136 649.0 0 136 222 189 649.0 0 189 223 196 649.0 0 196 224 154 649.0 0 154 225 117 649.0 0 117 226 129 649.0 0 129 227 108 649.0 0 108 228 96 649.0 0 96 229 103 649.0 0 103 230 90 649.0 0 90 231 90 649.0 0 90 232 113 649.0 0 113 233 77 649.0 0 77 234 81 649.0 0 81 235 116 649.0 0 116 236 110 649.0 0 110 237 119 649.0 0 119 238 114 649.0 0 114 239 109 649.0 0 109 240 176 649.0 0 176 241 134 649.0 0 134 242 199 649.0 0 199 243 156 649.0 0 156 244 278 649.0 0 278 245 244 649.0 0 244 246 212 649.0 0 212 247 113 649.0 0 113 248 89 649.0 0 89 249 107 649.0 0 107 250 95 649.0 0 95 251 85 649.0 0 85 252 157 649.0 0 157 253 426 649.0 0 426 254 353 649.0 0 353 255 424 649.0 0 424 256 121 649.0 0 121 257 155 649.0 0 155 258 520 649.0 0 520 259 369 649.0 0 369 260 293 649.0 0 293 261 133 649.0 0 133 262 131 649.0 0 131 263 278 649.0 0 278 264 357 649.0 0 357 265 225 649.0 0 225 266 129 649.0 0 129 267 74 649.0 0 74 268 75 649.0 0 75 269 169 649.0 0 169 270 161 649.0 0 161 271 396 649.0 0 396 272 178 649.0 0 178 273 298 649.0 0 298 274 149 649.0 0 149 275 192 649.0 0 192 276 171 649.0 0 171 277 120 649.0 0 120 278 123 649.0 0 123 279 118 649.0 0 118 280 91 649.0 0 91 281 137 649.0 0 137 282 138 649.0 0 138 283 173 649.0 0 173 284 218 649.0 0 218 285 175 649.0 0 175 286 157 649.0 0 157 287 120 649.0 0 120 288 101 649.0 0 101 289 118 649.0 0 118 290 167 649.0 0 167 291 179 649.0 0 179 292 200 649.0 0 200 293 122 649.0 0 122 294 94 649.0 0 94 295 110 649.0 0 110 296 134 649.0 0 134 297 154 649.0 0 154 298 153 649.0 0 153 299 130 649.0 0 130 300 153 649.0 0 153 301 198 649.0 0 198 Finished in 2.00 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 44,310 Reads with adapters: 13,323 (30.1%) Reads that were too short: 58 (0.1%) Reads written (passing filters): 13,265 (29.9%) Total basepairs processed: 10,749,350 bp Total written (filtered): 3,651,881 bp (34.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 13323 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 12 0.0 3 0 0 8 4 16 168 0.0 3 0 138 20 10 17 12853 0.0 3 12229 521 80 23 18 32 0.0 3 4 22 2 4 19 35 0.0 3 0 1 24 10 20 22 0.0 3 0 0 0 22 21 1 0.0 3 0 0 0 1 23 1 0.0 3 0 0 0 1 24 3 0.0 3 0 0 0 3 28 1 0.0 3 0 0 0 1 29 9 0.0 3 0 0 0 9 31 1 0.0 3 0 0 0 1 34 3 0.0 3 0 0 0 3 37 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 58 2 0.0 3 0 0 0 2 60 100 0.0 3 0 0 2 98 61 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 67 2 0.0 3 0 0 0 2 69 2 0.0 3 0 0 0 2 74 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 81 2 0.0 3 0 0 0 2 83 1 0.0 3 0 0 0 1 84 2 0.0 3 0 0 0 2 85 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 98 1 0.0 3 1 102 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 1 171 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 217 25 0.0 3 0 0 1 24 218 2 0.0 3 0 0 0 2 221 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 2 0.0 3 0 0 0 2 281 2 0.0 3 0 0 0 2 282 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.48 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 13,265 Reads with adapters: 144 (1.1%) Reads written (passing filters): 13,265 (100.0%) Total basepairs processed: 3,651,881 bp Total written (filtered): 3,651,439 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 144 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 29.2% C: 45.8% G: 19.4% T: 5.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 136 207.3 0 136 4 7 51.8 0 7 6 1 3.2 0 1