This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTATAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 70.13 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 58,659 (2.2%) Reads that were too short: 15,411 (0.6%) Reads written (passing filters): 43,248 (1.6%) Total basepairs processed: 800,111,879 bp Total written (filtered): 10,560,647 bp (1.3%) === Adapter 1 === Sequence: CTATAA; Type: regular 5'; Length: 6; Trimmed: 58659 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 257 649.0 0 257 7 12672 649.0 0 12672 8 12817 649.0 0 12817 9 15 649.0 0 15 10 29 649.0 0 29 11 14 649.0 0 14 12 17 649.0 0 17 13 28 649.0 0 28 14 15 649.0 0 15 15 20 649.0 0 20 16 30 649.0 0 30 17 22 649.0 0 22 18 20 649.0 0 20 19 23 649.0 0 23 20 13 649.0 0 13 21 15 649.0 0 15 22 15 649.0 0 15 23 20 649.0 0 20 24 19 649.0 0 19 25 15 649.0 0 15 26 21 649.0 0 21 27 16 649.0 0 16 28 25 649.0 0 25 29 7 649.0 0 7 30 14 649.0 0 14 31 18 649.0 0 18 32 13 649.0 0 13 33 24 649.0 0 24 34 32 649.0 0 32 35 17 649.0 0 17 36 39 649.0 0 39 37 84 649.0 0 84 38 133 649.0 0 133 39 28 649.0 0 28 40 23 649.0 0 23 41 14 649.0 0 14 42 266 649.0 0 266 43 22 649.0 0 22 44 21 649.0 0 21 45 24 649.0 0 24 46 28 649.0 0 28 47 18 649.0 0 18 48 17 649.0 0 17 49 20 649.0 0 20 50 21 649.0 0 21 51 21 649.0 0 21 52 17 649.0 0 17 53 13 649.0 0 13 54 31 649.0 0 31 55 13 649.0 0 13 56 20 649.0 0 20 57 19 649.0 0 19 58 21 649.0 0 21 59 15 649.0 0 15 60 23 649.0 0 23 61 93 649.0 0 93 62 14 649.0 0 14 63 11 649.0 0 11 64 19 649.0 0 19 65 18 649.0 0 18 66 18 649.0 0 18 67 28 649.0 0 28 68 12 649.0 0 12 69 26 649.0 0 26 70 16 649.0 0 16 71 19 649.0 0 19 72 27 649.0 0 27 73 13 649.0 0 13 74 8 649.0 0 8 75 18 649.0 0 18 76 16 649.0 0 16 77 26 649.0 0 26 78 20 649.0 0 20 79 12 649.0 0 12 80 47 649.0 0 47 81 69 649.0 0 69 82 60 649.0 0 60 83 58 649.0 0 58 84 77 649.0 0 77 85 84 649.0 0 84 86 54 649.0 0 54 87 65 649.0 0 65 88 57 649.0 0 57 89 75 649.0 0 75 90 66 649.0 0 66 91 75 649.0 0 75 92 96 649.0 0 96 93 86 649.0 0 86 94 110 649.0 0 110 95 73 649.0 0 73 96 111 649.0 0 111 97 141 649.0 0 141 98 131 649.0 0 131 99 159 649.0 0 159 100 141 649.0 0 141 101 116 649.0 0 116 102 104 649.0 0 104 103 91 649.0 0 91 104 84 649.0 0 84 105 120 649.0 0 120 106 194 649.0 0 194 107 176 649.0 0 176 108 385 649.0 0 385 109 269 649.0 0 269 110 456 649.0 0 456 111 226 649.0 0 226 112 223 649.0 0 223 113 163 649.0 0 163 114 266 649.0 0 266 115 217 649.0 0 217 116 268 649.0 0 268 117 110 649.0 0 110 118 113 649.0 0 113 119 64 649.0 0 64 120 65 649.0 0 65 121 88 649.0 0 88 122 153 649.0 0 153 123 143 649.0 0 143 124 100 649.0 0 100 125 81 649.0 0 81 126 89 649.0 0 89 127 89 649.0 0 89 128 120 649.0 0 120 129 183 649.0 0 183 130 190 649.0 0 190 131 221 649.0 0 221 132 180 649.0 0 180 133 102 649.0 0 102 134 100 649.0 0 100 135 117 649.0 0 117 136 178 649.0 0 178 137 251 649.0 0 251 138 141 649.0 0 141 139 124 649.0 0 124 140 74 649.0 0 74 141 113 649.0 0 113 142 146 649.0 0 146 143 114 649.0 0 114 144 112 649.0 0 112 145 112 649.0 0 112 146 91 649.0 0 91 147 89 649.0 0 89 148 124 649.0 0 124 149 129 649.0 0 129 150 120 649.0 0 120 151 98 649.0 0 98 152 124 649.0 0 124 153 113 649.0 0 113 154 108 649.0 0 108 155 153 649.0 0 153 156 140 649.0 0 140 157 165 649.0 0 165 158 171 649.0 0 171 159 108 649.0 0 108 160 126 649.0 0 126 161 113 649.0 0 113 162 251 649.0 0 251 163 184 649.0 0 184 164 329 649.0 0 329 165 168 649.0 0 168 166 125 649.0 0 125 167 125 649.0 0 125 168 106 649.0 0 106 169 126 649.0 0 126 170 129 649.0 0 129 171 97 649.0 0 97 172 87 649.0 0 87 173 67 649.0 0 67 174 91 649.0 0 91 175 91 649.0 0 91 176 133 649.0 0 133 177 102 649.0 0 102 178 122 649.0 0 122 179 92 649.0 0 92 180 123 649.0 0 123 181 108 649.0 0 108 182 112 649.0 0 112 183 98 649.0 0 98 184 97 649.0 0 97 185 82 649.0 0 82 186 116 649.0 0 116 187 81 649.0 0 81 188 119 649.0 0 119 189 116 649.0 0 116 190 134 649.0 0 134 191 126 649.0 0 126 192 115 649.0 0 115 193 89 649.0 0 89 194 118 649.0 0 118 195 99 649.0 0 99 196 97 649.0 0 97 197 85 649.0 0 85 198 105 649.0 0 105 199 77 649.0 0 77 200 120 649.0 0 120 201 140 649.0 0 140 202 221 649.0 0 221 203 159 649.0 0 159 204 137 649.0 0 137 205 116 649.0 0 116 206 108 649.0 0 108 207 123 649.0 0 123 208 166 649.0 0 166 209 199 649.0 0 199 210 194 649.0 0 194 211 135 649.0 0 135 212 74 649.0 0 74 213 95 649.0 0 95 214 90 649.0 0 90 215 97 649.0 0 97 216 113 649.0 0 113 217 106 649.0 0 106 218 111 649.0 0 111 219 97 649.0 0 97 220 99 649.0 0 99 221 101 649.0 0 101 222 152 649.0 0 152 223 197 649.0 0 197 224 155 649.0 0 155 225 93 649.0 0 93 226 108 649.0 0 108 227 109 649.0 0 109 228 89 649.0 0 89 229 97 649.0 0 97 230 103 649.0 0 103 231 68 649.0 0 68 232 113 649.0 0 113 233 87 649.0 0 87 234 93 649.0 0 93 235 104 649.0 0 104 236 107 649.0 0 107 237 130 649.0 0 130 238 111 649.0 0 111 239 104 649.0 0 104 240 142 649.0 0 142 241 150 649.0 0 150 242 240 649.0 0 240 243 170 649.0 0 170 244 243 649.0 0 243 245 239 649.0 0 239 246 207 649.0 0 207 247 108 649.0 0 108 248 96 649.0 0 96 249 97 649.0 0 97 250 108 649.0 0 108 251 106 649.0 0 106 252 169 649.0 0 169 253 374 649.0 0 374 254 307 649.0 0 307 255 341 649.0 0 341 256 115 649.0 0 115 257 147 649.0 0 147 258 572 649.0 0 572 259 368 649.0 0 368 260 331 649.0 0 331 261 130 649.0 0 130 262 135 649.0 0 135 263 235 649.0 0 235 264 477 649.0 0 477 265 225 649.0 0 225 266 199 649.0 0 199 267 73 649.0 0 73 268 76 649.0 0 76 269 131 649.0 0 131 270 132 649.0 0 132 271 326 649.0 0 326 272 168 649.0 0 168 273 287 649.0 0 287 274 132 649.0 0 132 275 211 649.0 0 211 276 116 649.0 0 116 277 128 649.0 0 128 278 95 649.0 0 95 279 79 649.0 0 79 280 87 649.0 0 87 281 133 649.0 0 133 282 127 649.0 0 127 283 165 649.0 0 165 284 142 649.0 0 142 285 139 649.0 0 139 286 122 649.0 0 122 287 76 649.0 0 76 288 102 649.0 0 102 289 129 649.0 0 129 290 162 649.0 0 162 291 160 649.0 0 160 292 172 649.0 0 172 293 109 649.0 0 109 294 87 649.0 0 87 295 97 649.0 0 97 296 128 649.0 0 128 297 152 649.0 0 152 298 178 649.0 0 178 299 132 649.0 0 132 300 116 649.0 0 116 301 150 649.0 0 150 Finished in 2.16 s (50 us/read; 1.20 M reads/minute). === Summary === Total reads processed: 43,248 Reads with adapters: 13,313 (30.8%) Reads that were too short: 7 (0.0%) Reads written (passing filters): 13,306 (30.8%) Total basepairs processed: 10,560,647 bp Total written (filtered): 3,607,554 bp (34.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 13313 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 4 0.0 3 0 0 4 19 107 0.0 3 0 104 3 20 12897 0.0 4 12688 199 8 1 1 21 45 0.0 4 3 39 2 1 22 4 0.0 4 0 0 4 23 9 0.0 4 0 0 0 8 1 24 3 0.0 4 0 0 0 0 3 26 5 0.0 4 0 0 0 0 5 37 1 0.0 4 0 0 0 0 1 40 1 0.0 4 1 46 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 1 65 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 3 0.0 4 0 0 0 0 3 127 218 0.0 4 0 0 0 0 218 128 1 0.0 4 0 0 0 0 1 129 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 0 0 1 133 1 0.0 4 0 0 0 1 143 1 0.0 4 1 156 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.47 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 13,306 Reads with adapters: 1,335 (10.0%) Reads written (passing filters): 13,306 (100.0%) Total basepairs processed: 3,607,554 bp Total written (filtered): 3,592,568 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1335 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.8% C: 2.9% G: 93.4% T: 1.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 8 207.9 0 8 4 3 52.0 0 3 9 6 0.1 0 1 5 10 190 0.0 1 8 182 11 1119 0.0 1 6 1113 12 2 0.0 1 0 2 13 2 0.0 1 0 2 19 2 0.0 1 2 151 1 0.0 1 0 1 224 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.