This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGCGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 69.25 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 83,650 (3.1%) Reads that were too short: 20,137 (0.8%) Reads written (passing filters): 63,513 (2.4%) Total basepairs processed: 800,111,879 bp Total written (filtered): 15,919,353 bp (2.0%) === Adapter 1 === Sequence: TGGCGG; Type: regular 5'; Length: 6; Trimmed: 83650 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1955 649.0 0 1955 7 18654 649.0 0 18654 8 467 649.0 0 467 9 17812 649.0 0 17812 10 71 649.0 0 71 11 63 649.0 0 63 12 65 649.0 0 65 13 66 649.0 0 66 14 44 649.0 0 44 15 53 649.0 0 53 16 38 649.0 0 38 17 69 649.0 0 69 18 87 649.0 0 87 19 82 649.0 0 82 20 51 649.0 0 51 21 54 649.0 0 54 22 39 649.0 0 39 23 52 649.0 0 52 24 36 649.0 0 36 25 43 649.0 0 43 26 54 649.0 0 54 27 58 649.0 0 58 28 42 649.0 0 42 29 48 649.0 0 48 30 43 649.0 0 43 31 60 649.0 0 60 32 50 649.0 0 50 33 56 649.0 0 56 34 50 649.0 0 50 35 53 649.0 0 53 36 50 649.0 0 50 37 73 649.0 0 73 38 59 649.0 0 59 39 52 649.0 0 52 40 57 649.0 0 57 41 68 649.0 0 68 42 69 649.0 0 69 43 66 649.0 0 66 44 48 649.0 0 48 45 74 649.0 0 74 46 87 649.0 0 87 47 516 649.0 0 516 48 208 649.0 0 208 49 532 649.0 0 532 50 62 649.0 0 62 51 46 649.0 0 46 52 50 649.0 0 50 53 93 649.0 0 93 54 113 649.0 0 113 55 77 649.0 0 77 56 81 649.0 0 81 57 68 649.0 0 68 58 74 649.0 0 74 59 206 649.0 0 206 60 121 649.0 0 121 61 233 649.0 0 233 62 211 649.0 0 211 63 84 649.0 0 84 64 46 649.0 0 46 65 58 649.0 0 58 66 54 649.0 0 54 67 49 649.0 0 49 68 51 649.0 0 51 69 55 649.0 0 55 70 69 649.0 0 69 71 80 649.0 0 80 72 82 649.0 0 82 73 56 649.0 0 56 74 44 649.0 0 44 75 70 649.0 0 70 76 78 649.0 0 78 77 115 649.0 0 115 78 67 649.0 0 67 79 80 649.0 0 80 80 60 649.0 0 60 81 69 649.0 0 69 82 55 649.0 0 55 83 119 649.0 0 119 84 232 649.0 0 232 85 136 649.0 0 136 86 260 649.0 0 260 87 46 649.0 0 46 88 48 649.0 0 48 89 60 649.0 0 60 90 53 649.0 0 53 91 57 649.0 0 57 92 65 649.0 0 65 93 58 649.0 0 58 94 66 649.0 0 66 95 62 649.0 0 62 96 65 649.0 0 65 97 55 649.0 0 55 98 43 649.0 0 43 99 51 649.0 0 51 100 58 649.0 0 58 101 43 649.0 0 43 102 55 649.0 0 55 103 49 649.0 0 49 104 44 649.0 0 44 105 47 649.0 0 47 106 66 649.0 0 66 107 56 649.0 0 56 108 62 649.0 0 62 109 77 649.0 0 77 110 58 649.0 0 58 111 49 649.0 0 49 112 205 649.0 0 205 113 351 649.0 0 351 114 264 649.0 0 264 115 70 649.0 0 70 116 48 649.0 0 48 117 66 649.0 0 66 118 76 649.0 0 76 119 66 649.0 0 66 120 53 649.0 0 53 121 85 649.0 0 85 122 52 649.0 0 52 123 59 649.0 0 59 124 49 649.0 0 49 125 55 649.0 0 55 126 75 649.0 0 75 127 119 649.0 0 119 128 106 649.0 0 106 129 175 649.0 0 175 130 198 649.0 0 198 131 140 649.0 0 140 132 185 649.0 0 185 133 208 649.0 0 208 134 175 649.0 0 175 135 130 649.0 0 130 136 219 649.0 0 219 137 105 649.0 0 105 138 122 649.0 0 122 139 99 649.0 0 99 140 395 649.0 0 395 141 4395 649.0 0 4395 142 827 649.0 0 827 143 2572 649.0 0 2572 144 68 649.0 0 68 145 40 649.0 0 40 146 77 649.0 0 77 147 104 649.0 0 104 148 93 649.0 0 93 149 117 649.0 0 117 150 52 649.0 0 52 151 76 649.0 0 76 152 211 649.0 0 211 153 233 649.0 0 233 154 210 649.0 0 210 155 139 649.0 0 139 156 56 649.0 0 56 157 50 649.0 0 50 158 72 649.0 0 72 159 66 649.0 0 66 160 54 649.0 0 54 161 60 649.0 0 60 162 54 649.0 0 54 163 64 649.0 0 64 164 72 649.0 0 72 165 63 649.0 0 63 166 65 649.0 0 65 167 57 649.0 0 57 168 58 649.0 0 58 169 62 649.0 0 62 170 57 649.0 0 57 171 78 649.0 0 78 172 72 649.0 0 72 173 74 649.0 0 74 174 82 649.0 0 82 175 107 649.0 0 107 176 62 649.0 0 62 177 43 649.0 0 43 178 46 649.0 0 46 179 45 649.0 0 45 180 50 649.0 0 50 181 57 649.0 0 57 182 48 649.0 0 48 183 62 649.0 0 62 184 75 649.0 0 75 185 153 649.0 0 153 186 125 649.0 0 125 187 232 649.0 0 232 188 68 649.0 0 68 189 67 649.0 0 67 190 43 649.0 0 43 191 62 649.0 0 62 192 56 649.0 0 56 193 72 649.0 0 72 194 46 649.0 0 46 195 62 649.0 0 62 196 57 649.0 0 57 197 49 649.0 0 49 198 61 649.0 0 61 199 58 649.0 0 58 200 51 649.0 0 51 201 105 649.0 0 105 202 55 649.0 0 55 203 149 649.0 0 149 204 37 649.0 0 37 205 53 649.0 0 53 206 36 649.0 0 36 207 61 649.0 0 61 208 61 649.0 0 61 209 81 649.0 0 81 210 73 649.0 0 73 211 180 649.0 0 180 212 127 649.0 0 127 213 145 649.0 0 145 214 77 649.0 0 77 215 73 649.0 0 73 216 131 649.0 0 131 217 73 649.0 0 73 218 80 649.0 0 80 219 55 649.0 0 55 220 70 649.0 0 70 221 49 649.0 0 49 222 38 649.0 0 38 223 36 649.0 0 36 224 73 649.0 0 73 225 110 649.0 0 110 226 100 649.0 0 100 227 105 649.0 0 105 228 727 649.0 0 727 229 1516 649.0 0 1516 230 432 649.0 0 432 231 102 649.0 0 102 232 109 649.0 0 109 233 44 649.0 0 44 234 59 649.0 0 59 235 215 649.0 0 215 236 151 649.0 0 151 237 268 649.0 0 268 238 130 649.0 0 130 239 166 649.0 0 166 240 84 649.0 0 84 241 58 649.0 0 58 242 49 649.0 0 49 243 69 649.0 0 69 244 42 649.0 0 42 245 81 649.0 0 81 246 100 649.0 0 100 247 128 649.0 0 128 248 128 649.0 0 128 249 79 649.0 0 79 250 116 649.0 0 116 251 75 649.0 0 75 252 125 649.0 0 125 253 85 649.0 0 85 254 78 649.0 0 78 255 217 649.0 0 217 256 399 649.0 0 399 257 515 649.0 0 515 258 269 649.0 0 269 259 90 649.0 0 90 260 69 649.0 0 69 261 87 649.0 0 87 262 57 649.0 0 57 263 48 649.0 0 48 264 58 649.0 0 58 265 45 649.0 0 45 266 64 649.0 0 64 267 44 649.0 0 44 268 46 649.0 0 46 269 40 649.0 0 40 270 73 649.0 0 73 271 52 649.0 0 52 272 64 649.0 0 64 273 309 649.0 0 309 274 389 649.0 0 389 275 321 649.0 0 321 276 60 649.0 0 60 277 47 649.0 0 47 278 40 649.0 0 40 279 59 649.0 0 59 280 81 649.0 0 81 281 93 649.0 0 93 282 81 649.0 0 81 283 118 649.0 0 118 284 133 649.0 0 133 285 206 649.0 0 206 286 114 649.0 0 114 287 168 649.0 0 168 288 129 649.0 0 129 289 85 649.0 0 85 290 94 649.0 0 94 291 85 649.0 0 85 292 153 649.0 0 153 293 189 649.0 0 189 294 124 649.0 0 124 295 128 649.0 0 128 296 188 649.0 0 188 297 211 649.0 0 211 298 2008 649.0 0 2008 299 1292 649.0 0 1292 300 3381 649.0 0 3381 301 270 649.0 0 270 Finished in 3.24 s (51 us/read; 1.18 M reads/minute). === Summary === Total reads processed: 63,513 Reads with adapters: 19,880 (31.3%) Reads that were too short: 9 (0.0%) Reads written (passing filters): 19,871 (31.3%) Total basepairs processed: 15,919,353 bp Total written (filtered): 5,431,512 bp (34.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 19880 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 15 0.0 3 0 0 13 2 19 226 0.0 3 0 218 8 20 19173 0.0 4 18675 478 17 3 21 67 0.0 4 22 43 2 22 246 0.0 4 240 3 3 23 14 0.0 4 3 0 0 10 1 24 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 119 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 118 0.0 4 0 0 0 0 118 130 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 163 1 0.0 4 1 164 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 0 3 263 1 0.0 4 0 0 0 0 1 268 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.69 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 19,871 Reads with adapters: 624 (3.1%) Reads written (passing filters): 19,871 (100.0%) Total basepairs processed: 5,431,512 bp Total written (filtered): 5,424,765 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 624 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 7.5% C: 4.2% G: 86.7% T: 1.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 52 310.5 0 52 4 2 77.6 0 2 7 1 1.2 0 1 9 13 0.1 0 1 12 10 18 0.0 1 0 18 11 504 0.0 1 25 479 12 25 0.0 1 2 23 13 5 0.0 1 1 4 15 1 0.0 1 1 16 1 0.0 1 0 1 96 1 0.0 1 0 1 243 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.