This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGACCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.55 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 143,555 (5.4%) Reads that were too short: 40,790 (1.5%) Reads written (passing filters): 102,765 (3.9%) Total basepairs processed: 800,111,879 bp Total written (filtered): 28,466,461 bp (3.6%) === Adapter 1 === Sequence: AGACCG; Type: regular 5'; Length: 6; Trimmed: 143555 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1483 649.0 0 1483 7 54383 649.0 0 54383 8 727 649.0 0 727 9 33122 649.0 0 33122 10 205 649.0 0 205 11 26 649.0 0 26 12 29 649.0 0 29 13 14 649.0 0 14 14 15 649.0 0 15 15 13 649.0 0 13 16 23 649.0 0 23 17 17 649.0 0 17 18 15 649.0 0 15 19 8 649.0 0 8 20 11 649.0 0 11 21 14 649.0 0 14 22 6 649.0 0 6 23 7 649.0 0 7 24 8 649.0 0 8 25 17 649.0 0 17 26 27 649.0 0 27 27 9 649.0 0 9 28 15 649.0 0 15 29 27 649.0 0 27 30 17 649.0 0 17 31 17 649.0 0 17 32 23 649.0 0 23 33 17 649.0 0 17 34 15 649.0 0 15 35 18 649.0 0 18 36 11 649.0 0 11 37 17 649.0 0 17 38 16 649.0 0 16 39 16 649.0 0 16 40 20 649.0 0 20 41 11 649.0 0 11 42 15 649.0 0 15 43 14 649.0 0 14 44 14 649.0 0 14 45 14 649.0 0 14 46 12 649.0 0 12 47 14 649.0 0 14 48 21 649.0 0 21 49 19 649.0 0 19 50 19 649.0 0 19 51 13 649.0 0 13 52 18 649.0 0 18 53 29 649.0 0 29 54 43 649.0 0 43 55 95 649.0 0 95 56 56 649.0 0 56 57 16 649.0 0 16 58 12 649.0 0 12 59 28 649.0 0 28 60 28 649.0 0 28 61 45 649.0 0 45 62 22 649.0 0 22 63 20 649.0 0 20 64 19 649.0 0 19 65 12 649.0 0 12 66 21 649.0 0 21 67 17 649.0 0 17 68 14 649.0 0 14 69 29 649.0 0 29 70 36 649.0 0 36 71 109 649.0 0 109 72 39 649.0 0 39 73 45 649.0 0 45 74 156 649.0 0 156 75 128 649.0 0 128 76 82 649.0 0 82 77 21 649.0 0 21 78 31 649.0 0 31 79 82 649.0 0 82 80 95 649.0 0 95 81 132 649.0 0 132 82 252 649.0 0 252 83 201 649.0 0 201 84 171 649.0 0 171 85 99 649.0 0 99 86 65 649.0 0 65 87 35 649.0 0 35 88 51 649.0 0 51 89 66 649.0 0 66 90 25 649.0 0 25 91 37 649.0 0 37 92 63 649.0 0 63 93 35 649.0 0 35 94 27 649.0 0 27 95 29 649.0 0 29 96 15 649.0 0 15 97 28 649.0 0 28 98 21 649.0 0 21 99 29 649.0 0 29 100 25 649.0 0 25 101 21 649.0 0 21 102 37 649.0 0 37 103 29 649.0 0 29 104 19 649.0 0 19 105 28 649.0 0 28 106 30 649.0 0 30 107 34 649.0 0 34 108 12 649.0 0 12 109 21 649.0 0 21 110 30 649.0 0 30 111 148 649.0 0 148 112 31 649.0 0 31 113 35 649.0 0 35 114 38 649.0 0 38 115 34 649.0 0 34 116 35 649.0 0 35 117 31 649.0 0 31 118 37 649.0 0 37 119 38 649.0 0 38 120 39 649.0 0 39 121 111 649.0 0 111 122 110 649.0 0 110 123 405 649.0 0 405 124 50 649.0 0 50 125 112 649.0 0 112 126 103 649.0 0 103 127 82 649.0 0 82 128 63 649.0 0 63 129 41 649.0 0 41 130 59 649.0 0 59 131 28 649.0 0 28 132 26 649.0 0 26 133 61 649.0 0 61 134 30 649.0 0 30 135 50 649.0 0 50 136 49 649.0 0 49 137 56 649.0 0 56 138 54 649.0 0 54 139 33 649.0 0 33 140 48 649.0 0 48 141 33 649.0 0 33 142 138 649.0 0 138 143 37 649.0 0 37 144 66 649.0 0 66 145 39 649.0 0 39 146 48 649.0 0 48 147 64 649.0 0 64 148 54 649.0 0 54 149 39 649.0 0 39 150 41 649.0 0 41 151 52 649.0 0 52 152 53 649.0 0 53 153 63 649.0 0 63 154 54 649.0 0 54 155 44 649.0 0 44 156 47 649.0 0 47 157 37 649.0 0 37 158 38 649.0 0 38 159 45 649.0 0 45 160 38 649.0 0 38 161 43 649.0 0 43 162 54 649.0 0 54 163 73 649.0 0 73 164 63 649.0 0 63 165 79 649.0 0 79 166 96 649.0 0 96 167 1118 649.0 0 1118 168 324 649.0 0 324 169 442 649.0 0 442 170 124 649.0 0 124 171 241 649.0 0 241 172 199 649.0 0 199 173 153 649.0 0 153 174 56 649.0 0 56 175 61 649.0 0 61 176 32 649.0 0 32 177 58 649.0 0 58 178 42 649.0 0 42 179 49 649.0 0 49 180 79 649.0 0 79 181 48 649.0 0 48 182 131 649.0 0 131 183 81 649.0 0 81 184 130 649.0 0 130 185 77 649.0 0 77 186 88 649.0 0 88 187 80 649.0 0 80 188 86 649.0 0 86 189 122 649.0 0 122 190 72 649.0 0 72 191 80 649.0 0 80 192 49 649.0 0 49 193 49 649.0 0 49 194 78 649.0 0 78 195 180 649.0 0 180 196 61 649.0 0 61 197 92 649.0 0 92 198 79 649.0 0 79 199 185 649.0 0 185 200 419 649.0 0 419 201 536 649.0 0 536 202 227 649.0 0 227 203 92 649.0 0 92 204 65 649.0 0 65 205 103 649.0 0 103 206 78 649.0 0 78 207 66 649.0 0 66 208 34 649.0 0 34 209 34 649.0 0 34 210 57 649.0 0 57 211 77 649.0 0 77 212 63 649.0 0 63 213 83 649.0 0 83 214 127 649.0 0 127 215 100 649.0 0 100 216 178 649.0 0 178 217 96 649.0 0 96 218 95 649.0 0 95 219 103 649.0 0 103 220 118 649.0 0 118 221 118 649.0 0 118 222 108 649.0 0 108 223 166 649.0 0 166 224 271 649.0 0 271 225 325 649.0 0 325 226 471 649.0 0 471 227 1122 649.0 0 1122 228 4677 649.0 0 4677 229 12156 649.0 0 12156 230 5385 649.0 0 5385 231 217 649.0 0 217 232 123 649.0 0 123 233 60 649.0 0 60 234 44 649.0 0 44 235 54 649.0 0 54 236 54 649.0 0 54 237 94 649.0 0 94 238 82 649.0 0 82 239 87 649.0 0 87 240 80 649.0 0 80 241 59 649.0 0 59 242 63 649.0 0 63 243 190 649.0 0 190 244 128 649.0 0 128 245 263 649.0 0 263 246 216 649.0 0 216 247 306 649.0 0 306 248 338 649.0 0 338 249 162 649.0 0 162 250 130 649.0 0 130 251 123 649.0 0 123 252 96 649.0 0 96 253 69 649.0 0 69 254 70 649.0 0 70 255 124 649.0 0 124 256 443 649.0 0 443 257 321 649.0 0 321 258 616 649.0 0 616 259 110 649.0 0 110 260 119 649.0 0 119 261 325 649.0 0 325 262 387 649.0 0 387 263 605 649.0 0 605 264 344 649.0 0 344 265 283 649.0 0 283 266 185 649.0 0 185 267 173 649.0 0 173 268 699 649.0 0 699 269 617 649.0 0 617 270 631 649.0 0 631 271 215 649.0 0 215 272 82 649.0 0 82 273 74 649.0 0 74 274 110 649.0 0 110 275 109 649.0 0 109 276 100 649.0 0 100 277 91 649.0 0 91 278 88 649.0 0 88 279 74 649.0 0 74 280 109 649.0 0 109 281 240 649.0 0 240 282 213 649.0 0 213 283 304 649.0 0 304 284 87 649.0 0 87 285 110 649.0 0 110 286 117 649.0 0 117 287 134 649.0 0 134 288 164 649.0 0 164 289 133 649.0 0 133 290 89 649.0 0 89 291 105 649.0 0 105 292 353 649.0 0 353 293 338 649.0 0 338 294 375 649.0 0 375 295 141 649.0 0 141 296 103 649.0 0 103 297 79 649.0 0 79 298 79 649.0 0 79 299 166 649.0 0 166 300 437 649.0 0 437 301 286 649.0 0 286 Finished in 4.91 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 102,765 Reads with adapters: 55,191 (53.7%) Reads that were too short: 69 (0.1%) Reads written (passing filters): 55,122 (53.6%) Total basepairs processed: 28,466,461 bp Total written (filtered): 15,240,336 bp (53.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 55191 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 23 0.0 3 0 0 16 7 16 546 0.0 3 0 456 61 29 17 53918 0.0 3 51247 2231 361 79 18 161 0.0 3 18 115 19 9 19 105 0.0 3 0 0 62 43 20 151 0.0 3 1 0 0 150 21 1 0.0 3 0 0 0 1 22 2 0.0 3 1 0 0 1 24 13 0.0 3 0 0 0 13 27 2 0.0 3 0 0 0 2 28 2 0.0 3 0 0 0 2 29 1 0.0 3 0 0 0 1 31 108 0.0 3 0 0 0 108 34 1 0.0 3 0 0 0 1 39 2 0.0 3 0 0 0 2 43 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 46 3 0.0 3 0 0 0 3 47 1 0.0 3 0 0 0 1 50 3 0.0 3 0 0 0 3 52 1 0.0 3 0 0 0 1 53 3 0.0 3 0 0 1 2 54 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 65 3 0.0 3 0 0 0 3 69 2 0.0 3 0 0 0 2 70 4 0.0 3 0 0 0 4 71 10 0.0 3 0 0 0 10 74 3 0.0 3 0 0 1 2 80 2 0.0 3 0 0 0 2 81 2 0.0 3 0 0 0 2 82 1 0.0 3 0 0 0 1 84 2 0.0 3 0 0 0 2 86 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 88 2 0.0 3 0 0 0 2 90 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 101 3 0.0 3 1 0 1 1 104 1 0.0 3 0 0 1 105 1 0.0 3 0 0 1 108 4 0.0 3 0 0 0 4 109 1 0.0 3 0 0 0 1 114 2 0.0 3 0 0 0 2 116 1 0.0 3 0 0 0 1 117 2 0.0 3 0 0 0 2 119 2 0.0 3 0 0 0 2 120 1 0.0 3 0 0 0 1 121 3 0.0 3 0 0 0 3 123 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 131 2 0.0 3 0 0 0 2 133 2 0.0 3 0 0 0 2 136 1 0.0 3 0 0 0 1 137 2 0.0 3 0 0 0 2 139 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 145 2 0.0 3 0 0 0 2 147 1 0.0 3 0 0 0 1 150 2 0.0 3 0 0 0 2 156 2 0.0 3 0 0 0 2 157 1 0.0 3 0 0 0 1 161 2 0.0 3 0 0 0 2 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 182 2 0.0 3 0 0 0 2 183 1 0.0 3 0 0 0 1 184 2 0.0 3 0 0 0 2 192 3 0.0 3 0 0 0 3 193 1 0.0 3 0 0 0 1 194 2 0.0 3 0 0 0 2 202 1 0.0 3 0 0 0 1 217 6 0.0 3 0 0 0 6 218 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 233 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 1 268 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 272 1 0.0 3 0 0 0 1 279 4 0.0 3 0 0 0 4 280 4 0.0 3 0 0 0 4 281 2 0.0 3 0 0 0 2 282 4 0.0 3 0 0 0 4 286 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.93 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 55,122 Reads with adapters: 845 (1.5%) Reads written (passing filters): 55,122 (100.0%) Total basepairs processed: 15,240,336 bp Total written (filtered): 15,237,721 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 845 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 31.4% C: 28.3% G: 25.0% T: 15.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 776 861.3 0 776 4 60 215.3 0 60 5 7 53.8 0 7 6 2 13.5 0 2