This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 71.89 s (27 us/read; 2.22 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 176,279 (6.6%) Reads that were too short: 30,078 (1.1%) Reads written (passing filters): 146,201 (5.5%) Total basepairs processed: 800,111,879 bp Total written (filtered): 34,143,304 bp (4.3%) === Adapter 1 === Sequence: GTAACA; Type: regular 5'; Length: 6; Trimmed: 176279 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1890 649.0 0 1890 7 544 649.0 0 544 8 21113 649.0 0 21113 9 18916 649.0 0 18916 10 52 649.0 0 52 11 30 649.0 0 30 12 30 649.0 0 30 13 23 649.0 0 23 14 39 649.0 0 39 15 31 649.0 0 31 16 36 649.0 0 36 17 28 649.0 0 28 18 41 649.0 0 41 19 41 649.0 0 41 20 26 649.0 0 26 21 18 649.0 0 18 22 27 649.0 0 27 23 45 649.0 0 45 24 63 649.0 0 63 25 39 649.0 0 39 26 41 649.0 0 41 27 28 649.0 0 28 28 16 649.0 0 16 29 27 649.0 0 27 30 38 649.0 0 38 31 35 649.0 0 35 32 38 649.0 0 38 33 32 649.0 0 32 34 38 649.0 0 38 35 46 649.0 0 46 36 33 649.0 0 33 37 40 649.0 0 40 38 26 649.0 0 26 39 53 649.0 0 53 40 31 649.0 0 31 41 37 649.0 0 37 42 95 649.0 0 95 43 57 649.0 0 57 44 39 649.0 0 39 45 35 649.0 0 35 46 33 649.0 0 33 47 36 649.0 0 36 48 37 649.0 0 37 49 28 649.0 0 28 50 45 649.0 0 45 51 82 649.0 0 82 52 191 649.0 0 191 53 87 649.0 0 87 54 2405 649.0 0 2405 55 96 649.0 0 96 56 64 649.0 0 64 57 113 649.0 0 113 58 70 649.0 0 70 59 71 649.0 0 71 60 81 649.0 0 81 61 65 649.0 0 65 62 131 649.0 0 131 63 168 649.0 0 168 64 238 649.0 0 238 65 495 649.0 0 495 66 515 649.0 0 515 67 995 649.0 0 995 68 1534 649.0 0 1534 69 20135 649.0 0 20135 70 29841 649.0 0 29841 71 12634 649.0 0 12634 72 80 649.0 0 80 73 34 649.0 0 34 74 36 649.0 0 36 75 91 649.0 0 91 76 99 649.0 0 99 77 211 649.0 0 211 78 60 649.0 0 60 79 71 649.0 0 71 80 333 649.0 0 333 81 281 649.0 0 281 82 188 649.0 0 188 83 75 649.0 0 75 84 61 649.0 0 61 85 33 649.0 0 33 86 61 649.0 0 61 87 85 649.0 0 85 88 61 649.0 0 61 89 74 649.0 0 74 90 160 649.0 0 160 91 96 649.0 0 96 92 75 649.0 0 75 93 111 649.0 0 111 94 69 649.0 0 69 95 94 649.0 0 94 96 345 649.0 0 345 97 151 649.0 0 151 98 88 649.0 0 88 99 138 649.0 0 138 100 736 649.0 0 736 101 777 649.0 0 777 102 2221 649.0 0 2221 103 202 649.0 0 202 104 114 649.0 0 114 105 79 649.0 0 79 106 67 649.0 0 67 107 67 649.0 0 67 108 102 649.0 0 102 109 55 649.0 0 55 110 91 649.0 0 91 111 88 649.0 0 88 112 77 649.0 0 77 113 114 649.0 0 114 114 87 649.0 0 87 115 138 649.0 0 138 116 103 649.0 0 103 117 133 649.0 0 133 118 176 649.0 0 176 119 138 649.0 0 138 120 102 649.0 0 102 121 124 649.0 0 124 122 148 649.0 0 148 123 180 649.0 0 180 124 130 649.0 0 130 125 125 649.0 0 125 126 159 649.0 0 159 127 134 649.0 0 134 128 135 649.0 0 135 129 133 649.0 0 133 130 200 649.0 0 200 131 285 649.0 0 285 132 1538 649.0 0 1538 133 584 649.0 0 584 134 997 649.0 0 997 135 138 649.0 0 138 136 573 649.0 0 573 137 467 649.0 0 467 138 513 649.0 0 513 139 186 649.0 0 186 140 163 649.0 0 163 141 365 649.0 0 365 142 99 649.0 0 99 143 144 649.0 0 144 144 139 649.0 0 139 145 123 649.0 0 123 146 125 649.0 0 125 147 153 649.0 0 153 148 154 649.0 0 154 149 131 649.0 0 131 150 144 649.0 0 144 151 127 649.0 0 127 152 370 649.0 0 370 153 177 649.0 0 177 154 256 649.0 0 256 155 138 649.0 0 138 156 145 649.0 0 145 157 252 649.0 0 252 158 237 649.0 0 237 159 233 649.0 0 233 160 188 649.0 0 188 161 163 649.0 0 163 162 163 649.0 0 163 163 221 649.0 0 221 164 180 649.0 0 180 165 202 649.0 0 202 166 172 649.0 0 172 167 717 649.0 0 717 168 444 649.0 0 444 169 186 649.0 0 186 170 215 649.0 0 215 171 167 649.0 0 167 172 214 649.0 0 214 173 136 649.0 0 136 174 155 649.0 0 155 175 163 649.0 0 163 176 326 649.0 0 326 177 936 649.0 0 936 178 660 649.0 0 660 179 1012 649.0 0 1012 180 399 649.0 0 399 181 252 649.0 0 252 182 529 649.0 0 529 183 494 649.0 0 494 184 702 649.0 0 702 185 331 649.0 0 331 186 282 649.0 0 282 187 230 649.0 0 230 188 172 649.0 0 172 189 188 649.0 0 188 190 215 649.0 0 215 191 171 649.0 0 171 192 291 649.0 0 291 193 132 649.0 0 132 194 177 649.0 0 177 195 175 649.0 0 175 196 171 649.0 0 171 197 395 649.0 0 395 198 282 649.0 0 282 199 294 649.0 0 294 200 161 649.0 0 161 201 179 649.0 0 179 202 709 649.0 0 709 203 755 649.0 0 755 204 822 649.0 0 822 205 641 649.0 0 641 206 151 649.0 0 151 207 133 649.0 0 133 208 131 649.0 0 131 209 174 649.0 0 174 210 199 649.0 0 199 211 256 649.0 0 256 212 238 649.0 0 238 213 241 649.0 0 241 214 162 649.0 0 162 215 157 649.0 0 157 216 195 649.0 0 195 217 276 649.0 0 276 218 205 649.0 0 205 219 217 649.0 0 217 220 147 649.0 0 147 221 147 649.0 0 147 222 358 649.0 0 358 223 571 649.0 0 571 224 595 649.0 0 595 225 446 649.0 0 446 226 198 649.0 0 198 227 197 649.0 0 197 228 309 649.0 0 309 229 230 649.0 0 230 230 149 649.0 0 149 231 116 649.0 0 116 232 129 649.0 0 129 233 168 649.0 0 168 234 154 649.0 0 154 235 119 649.0 0 119 236 138 649.0 0 138 237 176 649.0 0 176 238 231 649.0 0 231 239 232 649.0 0 232 240 179 649.0 0 179 241 199 649.0 0 199 242 481 649.0 0 481 243 579 649.0 0 579 244 707 649.0 0 707 245 432 649.0 0 432 246 179 649.0 0 179 247 155 649.0 0 155 248 136 649.0 0 136 249 142 649.0 0 142 250 203 649.0 0 203 251 865 649.0 0 865 252 366 649.0 0 366 253 664 649.0 0 664 254 179 649.0 0 179 255 343 649.0 0 343 256 334 649.0 0 334 257 303 649.0 0 303 258 227 649.0 0 227 259 256 649.0 0 256 260 225 649.0 0 225 261 196 649.0 0 196 262 202 649.0 0 202 263 249 649.0 0 249 264 263 649.0 0 263 265 466 649.0 0 466 266 328 649.0 0 328 267 253 649.0 0 253 268 176 649.0 0 176 269 295 649.0 0 295 270 325 649.0 0 325 271 553 649.0 0 553 272 394 649.0 0 394 273 404 649.0 0 404 274 347 649.0 0 347 275 289 649.0 0 289 276 408 649.0 0 408 277 459 649.0 0 459 278 483 649.0 0 483 279 340 649.0 0 340 280 194 649.0 0 194 281 201 649.0 0 201 282 181 649.0 0 181 283 253 649.0 0 253 284 357 649.0 0 357 285 320 649.0 0 320 286 273 649.0 0 273 287 236 649.0 0 236 288 205 649.0 0 205 289 161 649.0 0 161 290 198 649.0 0 198 291 377 649.0 0 377 292 558 649.0 0 558 293 344 649.0 0 344 294 414 649.0 0 414 295 192 649.0 0 192 296 177 649.0 0 177 297 198 649.0 0 198 298 222 649.0 0 222 299 286 649.0 0 286 300 300 649.0 0 300 301 375 649.0 0 375 Finished in 6.16 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 146,201 Reads with adapters: 23,139 (15.8%) Reads that were too short: 226 (0.2%) Reads written (passing filters): 22,913 (15.7%) Total basepairs processed: 34,143,304 bp Total written (filtered): 6,268,197 bp (18.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 23139 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 70 0.0 3 0 0 14 56 16 255 0.0 3 0 220 24 11 17 20571 0.0 3 19339 1039 156 37 18 71 0.0 3 16 43 7 5 19 75 0.0 3 2 14 35 24 20 1585 0.0 3 1426 80 23 56 21 2 0.0 3 0 1 0 1 22 4 0.0 3 0 0 1 3 23 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 30 3 0.0 3 0 0 0 3 31 1 0.0 3 0 0 0 1 32 6 0.0 3 0 0 0 6 34 19 0.0 3 0 0 0 19 35 2 0.0 3 0 0 0 2 37 9 0.0 3 0 0 0 9 38 2 0.0 3 0 0 0 2 39 2 0.0 3 0 0 0 2 40 7 0.0 3 0 0 0 7 41 2 0.0 3 0 0 0 2 42 3 0.0 3 0 0 0 3 43 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 47 2 0.0 3 0 0 0 2 48 1 0.0 3 0 0 0 1 49 3 0.0 3 0 0 0 3 52 1 0.0 3 0 0 0 1 53 2 0.0 3 0 0 0 2 55 40 0.0 3 0 0 0 40 56 3 0.0 3 0 0 0 3 57 2 0.0 3 0 0 0 2 58 7 0.0 3 0 0 0 7 59 2 0.0 3 0 0 0 2 60 27 0.0 3 0 0 0 27 61 5 0.0 3 0 0 0 5 62 2 0.0 3 0 0 0 2 63 2 0.0 3 0 0 0 2 64 4 0.0 3 1 0 0 3 65 1 0.0 3 1 66 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 71 2 0.0 3 0 0 0 2 72 2 0.0 3 0 0 0 2 73 2 0.0 3 0 0 0 2 74 2 0.0 3 0 0 0 2 75 3 0.0 3 0 0 0 3 76 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 81 2 0.0 3 0 0 0 2 82 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 84 2 0.0 3 0 0 1 1 85 6 0.0 3 0 0 1 5 86 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 90 3 0.0 3 0 0 0 3 91 84 0.0 3 0 0 1 83 92 3 0.0 3 0 0 0 3 94 21 0.0 3 0 0 1 20 95 4 0.0 3 0 0 0 4 96 2 0.0 3 0 0 0 2 98 3 0.0 3 0 0 0 3 99 2 0.0 3 0 0 0 2 100 1 0.0 3 0 0 0 1 101 12 0.0 3 0 0 1 11 102 1 0.0 3 0 0 0 1 103 2 0.0 3 0 0 0 2 104 7 0.0 3 0 0 0 7 106 4 0.0 3 0 0 0 4 107 1 0.0 3 0 0 0 1 108 2 0.0 3 0 0 0 2 109 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 111 2 0.0 3 0 0 0 2 114 1 0.0 3 0 0 0 1 115 2 0.0 3 0 0 0 2 116 1 0.0 3 0 0 0 1 117 5 0.0 3 0 0 0 5 119 3 0.0 3 0 0 0 3 120 2 0.0 3 0 0 0 2 121 3 0.0 3 0 0 0 3 122 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 124 9 0.0 3 0 0 0 9 125 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 130 3 0.0 3 0 0 0 3 132 1 0.0 3 0 0 1 135 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 142 2 0.0 3 0 0 0 2 143 2 0.0 3 0 0 0 2 144 1 0.0 3 0 0 0 1 146 2 0.0 3 0 0 0 2 147 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 150 5 0.0 3 0 0 0 5 151 4 0.0 3 0 0 0 4 154 2 0.0 3 0 0 0 2 156 7 0.0 3 0 0 0 7 158 1 0.0 3 0 0 0 1 159 7 0.0 3 0 0 0 7 164 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 168 2 0.0 3 0 0 0 2 171 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 182 2 0.0 3 0 0 0 2 183 3 0.0 3 0 0 0 3 188 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 6 0.0 3 0 0 1 5 193 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 207 2 0.0 3 0 0 0 2 214 1 0.0 3 0 0 0 1 217 12 0.0 3 0 0 0 12 218 2 0.0 3 0 0 0 2 219 12 0.0 3 0 0 0 12 220 3 0.0 3 0 0 0 3 221 1 0.0 3 0 0 0 1 226 2 0.0 3 0 0 1 1 234 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 1 278 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 280 4 0.0 3 0 0 0 4 281 3 0.0 3 0 0 0 3 282 3 0.0 3 0 0 0 3 283 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.80 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 22,913 Reads with adapters: 531 (2.3%) Reads written (passing filters): 22,913 (100.0%) Total basepairs processed: 6,268,197 bp Total written (filtered): 6,266,571 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 531 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 25.6% C: 11.5% G: 58.2% T: 4.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 506 358.0 0 506 4 20 89.5 0 20 5 3 22.4 0 3 6 1 5.6 0 1 7 1 1.4 0 1