This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 70.94 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 168,139 (6.3%) Reads that were too short: 19,599 (0.7%) Reads written (passing filters): 148,540 (5.6%) Total basepairs processed: 800,111,879 bp Total written (filtered): 35,387,976 bp (4.4%) === Adapter 1 === Sequence: GTAACA; Type: regular 5'; Length: 6; Trimmed: 168139 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1788 649.0 0 1788 7 448 649.0 0 448 8 20518 649.0 0 20518 9 20903 649.0 0 20903 10 42 649.0 0 42 11 38 649.0 0 38 12 42 649.0 0 42 13 44 649.0 0 44 14 40 649.0 0 40 15 44 649.0 0 44 16 36 649.0 0 36 17 31 649.0 0 31 18 47 649.0 0 47 19 36 649.0 0 36 20 35 649.0 0 35 21 38 649.0 0 38 22 47 649.0 0 47 23 36 649.0 0 36 24 26 649.0 0 26 25 39 649.0 0 39 26 37 649.0 0 37 27 36 649.0 0 36 28 20 649.0 0 20 29 45 649.0 0 45 30 54 649.0 0 54 31 50 649.0 0 50 32 59 649.0 0 59 33 1565 649.0 0 1565 34 114 649.0 0 114 35 57 649.0 0 57 36 51 649.0 0 51 37 44 649.0 0 44 38 34 649.0 0 34 39 60 649.0 0 60 40 26 649.0 0 26 41 52 649.0 0 52 42 125 649.0 0 125 43 49 649.0 0 49 44 44 649.0 0 44 45 49 649.0 0 49 46 51 649.0 0 51 47 33 649.0 0 33 48 33 649.0 0 33 49 39 649.0 0 39 50 63 649.0 0 63 51 83 649.0 0 83 52 164 649.0 0 164 53 91 649.0 0 91 54 152 649.0 0 152 55 56 649.0 0 56 56 85 649.0 0 85 57 110 649.0 0 110 58 98 649.0 0 98 59 74 649.0 0 74 60 73 649.0 0 73 61 86 649.0 0 86 62 141 649.0 0 141 63 165 649.0 0 165 64 240 649.0 0 240 65 472 649.0 0 472 66 530 649.0 0 530 67 1020 649.0 0 1020 68 1254 649.0 0 1254 69 22408 649.0 0 22408 70 34735 649.0 0 34735 71 15510 649.0 0 15510 72 94 649.0 0 94 73 41 649.0 0 41 74 40 649.0 0 40 75 46 649.0 0 46 76 61 649.0 0 61 77 34 649.0 0 34 78 41 649.0 0 41 79 55 649.0 0 55 80 237 649.0 0 237 81 183 649.0 0 183 82 214 649.0 0 214 83 89 649.0 0 89 84 103 649.0 0 103 85 73 649.0 0 73 86 73 649.0 0 73 87 93 649.0 0 93 88 94 649.0 0 94 89 81 649.0 0 81 90 92 649.0 0 92 91 86 649.0 0 86 92 86 649.0 0 86 93 103 649.0 0 103 94 116 649.0 0 116 95 121 649.0 0 121 96 89 649.0 0 89 97 105 649.0 0 105 98 86 649.0 0 86 99 93 649.0 0 93 100 289 649.0 0 289 101 379 649.0 0 379 102 782 649.0 0 782 103 122 649.0 0 122 104 119 649.0 0 119 105 93 649.0 0 93 106 94 649.0 0 94 107 87 649.0 0 87 108 116 649.0 0 116 109 88 649.0 0 88 110 115 649.0 0 115 111 143 649.0 0 143 112 105 649.0 0 105 113 115 649.0 0 115 114 100 649.0 0 100 115 119 649.0 0 119 116 90 649.0 0 90 117 114 649.0 0 114 118 164 649.0 0 164 119 121 649.0 0 121 120 130 649.0 0 130 121 113 649.0 0 113 122 135 649.0 0 135 123 164 649.0 0 164 124 147 649.0 0 147 125 104 649.0 0 104 126 112 649.0 0 112 127 93 649.0 0 93 128 99 649.0 0 99 129 110 649.0 0 110 130 183 649.0 0 183 131 246 649.0 0 246 132 1021 649.0 0 1021 133 476 649.0 0 476 134 765 649.0 0 765 135 138 649.0 0 138 136 339 649.0 0 339 137 322 649.0 0 322 138 405 649.0 0 405 139 161 649.0 0 161 140 159 649.0 0 159 141 381 649.0 0 381 142 117 649.0 0 117 143 119 649.0 0 119 144 140 649.0 0 140 145 125 649.0 0 125 146 91 649.0 0 91 147 99 649.0 0 99 148 114 649.0 0 114 149 131 649.0 0 131 150 131 649.0 0 131 151 117 649.0 0 117 152 297 649.0 0 297 153 164 649.0 0 164 154 216 649.0 0 216 155 86 649.0 0 86 156 117 649.0 0 117 157 189 649.0 0 189 158 192 649.0 0 192 159 180 649.0 0 180 160 165 649.0 0 165 161 173 649.0 0 173 162 114 649.0 0 114 163 193 649.0 0 193 164 179 649.0 0 179 165 213 649.0 0 213 166 172 649.0 0 172 167 790 649.0 0 790 168 475 649.0 0 475 169 171 649.0 0 171 170 138 649.0 0 138 171 139 649.0 0 139 172 161 649.0 0 161 173 124 649.0 0 124 174 135 649.0 0 135 175 135 649.0 0 135 176 259 649.0 0 259 177 538 649.0 0 538 178 460 649.0 0 460 179 549 649.0 0 549 180 327 649.0 0 327 181 171 649.0 0 171 182 255 649.0 0 255 183 211 649.0 0 211 184 399 649.0 0 399 185 223 649.0 0 223 186 208 649.0 0 208 187 192 649.0 0 192 188 157 649.0 0 157 189 183 649.0 0 183 190 166 649.0 0 166 191 150 649.0 0 150 192 255 649.0 0 255 193 112 649.0 0 112 194 138 649.0 0 138 195 133 649.0 0 133 196 144 649.0 0 144 197 219 649.0 0 219 198 149 649.0 0 149 199 198 649.0 0 198 200 126 649.0 0 126 201 114 649.0 0 114 202 283 649.0 0 283 203 315 649.0 0 315 204 362 649.0 0 362 205 237 649.0 0 237 206 129 649.0 0 129 207 133 649.0 0 133 208 108 649.0 0 108 209 115 649.0 0 115 210 139 649.0 0 139 211 187 649.0 0 187 212 170 649.0 0 170 213 159 649.0 0 159 214 126 649.0 0 126 215 156 649.0 0 156 216 142 649.0 0 142 217 182 649.0 0 182 218 147 649.0 0 147 219 158 649.0 0 158 220 102 649.0 0 102 221 107 649.0 0 107 222 163 649.0 0 163 223 304 649.0 0 304 224 360 649.0 0 360 225 302 649.0 0 302 226 194 649.0 0 194 227 204 649.0 0 204 228 266 649.0 0 266 229 211 649.0 0 211 230 123 649.0 0 123 231 155 649.0 0 155 232 142 649.0 0 142 233 143 649.0 0 143 234 175 649.0 0 175 235 133 649.0 0 133 236 116 649.0 0 116 237 136 649.0 0 136 238 129 649.0 0 129 239 147 649.0 0 147 240 135 649.0 0 135 241 147 649.0 0 147 242 226 649.0 0 226 243 260 649.0 0 260 244 360 649.0 0 360 245 234 649.0 0 234 246 161 649.0 0 161 247 135 649.0 0 135 248 121 649.0 0 121 249 141 649.0 0 141 250 183 649.0 0 183 251 411 649.0 0 411 252 230 649.0 0 230 253 297 649.0 0 297 254 146 649.0 0 146 255 154 649.0 0 154 256 227 649.0 0 227 257 181 649.0 0 181 258 145 649.0 0 145 259 170 649.0 0 170 260 200 649.0 0 200 261 141 649.0 0 141 262 161 649.0 0 161 263 203 649.0 0 203 264 206 649.0 0 206 265 281 649.0 0 281 266 216 649.0 0 216 267 158 649.0 0 158 268 136 649.0 0 136 269 200 649.0 0 200 270 249 649.0 0 249 271 364 649.0 0 364 272 208 649.0 0 208 273 233 649.0 0 233 274 234 649.0 0 234 275 185 649.0 0 185 276 238 649.0 0 238 277 254 649.0 0 254 278 229 649.0 0 229 279 226 649.0 0 226 280 124 649.0 0 124 281 173 649.0 0 173 282 165 649.0 0 165 283 185 649.0 0 185 284 210 649.0 0 210 285 190 649.0 0 190 286 224 649.0 0 224 287 173 649.0 0 173 288 170 649.0 0 170 289 137 649.0 0 137 290 161 649.0 0 161 291 212 649.0 0 212 292 334 649.0 0 334 293 236 649.0 0 236 294 270 649.0 0 270 295 165 649.0 0 165 296 149 649.0 0 149 297 156 649.0 0 156 298 191 649.0 0 191 299 245 649.0 0 245 300 265 649.0 0 265 301 218 649.0 0 218 Finished in 6.90 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 148,540 Reads with adapters: 21,949 (14.8%) Reads that were too short: 31 (0.0%) Reads written (passing filters): 21,918 (14.8%) Total basepairs processed: 35,387,976 bp Total written (filtered): 5,935,177 bp (16.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 21949 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 1 0.0 3 0 0 0 1 18 15 0.0 3 0 0 13 2 19 396 0.0 3 0 384 12 20 21174 0.0 4 20707 454 13 21 44 0.0 4 8 33 1 1 1 22 6 0.0 4 0 1 2 1 2 23 19 0.0 4 0 0 0 16 3 24 1 0.0 4 0 0 0 0 1 25 2 0.0 4 0 0 0 0 2 38 1 0.0 4 0 0 0 0 1 40 1 0.0 4 1 48 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 0 1 68 2 0.0 4 1 0 0 0 1 71 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 83 2 0.0 4 0 0 0 1 1 84 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 1 104 1 0.0 4 0 0 0 0 1 106 2 0.0 4 0 0 0 0 2 113 1 0.0 4 0 0 0 0 1 122 1 0.0 4 1 124 1 0.0 4 1 125 1 0.0 4 0 0 0 0 1 126 7 0.0 4 0 0 0 0 7 127 215 0.0 4 0 0 0 0 215 128 1 0.0 4 0 0 0 0 1 130 23 0.0 4 0 0 0 0 23 135 1 0.0 4 0 0 0 1 143 1 0.0 4 0 1 147 1 0.0 4 1 151 1 0.0 4 0 0 0 0 1 153 3 0.0 4 0 0 0 0 3 157 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 191 1 0.0 4 0 0 0 0 1 195 1 0.0 4 0 0 0 1 208 1 0.0 4 0 0 0 0 1 211 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 229 2 0.0 4 0 0 0 0 2 230 1 0.0 4 0 0 0 0 1 247 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.80 s (36 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 21,918 Reads with adapters: 3,571 (16.3%) Reads written (passing filters): 21,918 (100.0%) Total basepairs processed: 5,935,177 bp Total written (filtered): 5,831,128 bp (98.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3571 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.3% C: 9.8% G: 85.3% T: 2.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 8 342.5 0 8 4 3 85.6 0 3 5 8 21.4 0 8 6 3 5.4 0 3 9 1805 0.1 0 45 1760 10 988 0.0 1 35 953 11 382 0.0 1 5 377 12 2 0.0 1 0 2 13 1 0.0 1 0 1 18 32 0.0 1 25 7 19 9 0.0 1 6 3 21 1 0.0 1 1 22 1 0.0 1 0 1 77 1 0.0 1 1 138 1 0.0 1 0 1 150 1 0.0 1 0 1 169 1 0.0 1 1 177 1 0.0 1 1 222 1 0.0 1 1 223 307 0.0 1 287 20 224 11 0.0 1 9 2 225 1 0.0 1 1 226 1 0.0 1 0 1 235 1 0.0 1 1 257 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.