This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 68.95 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 101,043 (3.8%) Reads that were too short: 14,517 (0.5%) Reads written (passing filters): 86,526 (3.3%) Total basepairs processed: 800,111,879 bp Total written (filtered): 23,405,657 bp (2.9%) === Adapter 1 === Sequence: AATAGG; Type: regular 5'; Length: 6; Trimmed: 101043 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 507 649.0 0 507 7 1514 649.0 0 1514 8 50796 649.0 0 50796 9 15044 649.0 0 15044 10 58 649.0 0 58 11 92 649.0 0 92 12 7 649.0 0 7 13 24 649.0 0 24 14 16 649.0 0 16 15 41 649.0 0 41 16 28 649.0 0 28 17 43 649.0 0 43 18 51 649.0 0 51 19 56 649.0 0 56 20 26 649.0 0 26 21 17 649.0 0 17 22 18 649.0 0 18 23 18 649.0 0 18 24 22 649.0 0 22 25 28 649.0 0 28 26 22 649.0 0 22 27 25 649.0 0 25 28 162 649.0 0 162 29 146 649.0 0 146 30 151 649.0 0 151 31 28 649.0 0 28 32 21 649.0 0 21 33 22 649.0 0 22 34 21 649.0 0 21 35 21 649.0 0 21 36 28 649.0 0 28 37 26 649.0 0 26 38 22 649.0 0 22 39 19 649.0 0 19 40 28 649.0 0 28 41 22 649.0 0 22 42 35 649.0 0 35 43 55 649.0 0 55 44 105 649.0 0 105 45 590 649.0 0 590 46 162 649.0 0 162 47 268 649.0 0 268 48 14 649.0 0 14 49 19 649.0 0 19 50 26 649.0 0 26 51 21 649.0 0 21 52 15 649.0 0 15 53 17 649.0 0 17 54 19 649.0 0 19 55 12 649.0 0 12 56 19 649.0 0 19 57 18 649.0 0 18 58 13 649.0 0 13 59 12 649.0 0 12 60 15 649.0 0 15 61 20 649.0 0 20 62 18 649.0 0 18 63 21 649.0 0 21 64 19 649.0 0 19 65 16 649.0 0 16 66 22 649.0 0 22 67 17 649.0 0 17 68 20 649.0 0 20 69 39 649.0 0 39 70 69 649.0 0 69 71 152 649.0 0 152 72 106 649.0 0 106 73 160 649.0 0 160 74 98 649.0 0 98 75 51 649.0 0 51 76 143 649.0 0 143 77 35 649.0 0 35 78 54 649.0 0 54 79 71 649.0 0 71 80 90 649.0 0 90 81 90 649.0 0 90 82 451 649.0 0 451 83 172 649.0 0 172 84 189 649.0 0 189 85 292 649.0 0 292 86 165 649.0 0 165 87 32 649.0 0 32 88 59 649.0 0 59 89 68 649.0 0 68 90 36 649.0 0 36 91 57 649.0 0 57 92 66 649.0 0 66 93 90 649.0 0 90 94 1099 649.0 0 1099 95 584 649.0 0 584 96 1103 649.0 0 1103 97 259 649.0 0 259 98 37 649.0 0 37 99 20 649.0 0 20 100 36 649.0 0 36 101 43 649.0 0 43 102 18 649.0 0 18 103 36 649.0 0 36 104 38 649.0 0 38 105 24 649.0 0 24 106 41 649.0 0 41 107 35 649.0 0 35 108 28 649.0 0 28 109 35 649.0 0 35 110 31 649.0 0 31 111 52 649.0 0 52 112 62 649.0 0 62 113 385 649.0 0 385 114 535 649.0 0 535 115 433 649.0 0 433 116 270 649.0 0 270 117 60 649.0 0 60 118 47 649.0 0 47 119 48 649.0 0 48 120 49 649.0 0 49 121 46 649.0 0 46 122 71 649.0 0 71 123 65 649.0 0 65 124 60 649.0 0 60 125 61 649.0 0 61 126 44 649.0 0 44 127 52 649.0 0 52 128 75 649.0 0 75 129 71 649.0 0 71 130 65 649.0 0 65 131 69 649.0 0 69 132 84 649.0 0 84 133 114 649.0 0 114 134 136 649.0 0 136 135 411 649.0 0 411 136 478 649.0 0 478 137 265 649.0 0 265 138 360 649.0 0 360 139 227 649.0 0 227 140 159 649.0 0 159 141 89 649.0 0 89 142 84 649.0 0 84 143 75 649.0 0 75 144 79 649.0 0 79 145 57 649.0 0 57 146 54 649.0 0 54 147 40 649.0 0 40 148 47 649.0 0 47 149 55 649.0 0 55 150 43 649.0 0 43 151 37 649.0 0 37 152 59 649.0 0 59 153 42 649.0 0 42 154 49 649.0 0 49 155 72 649.0 0 72 156 50 649.0 0 50 157 80 649.0 0 80 158 45 649.0 0 45 159 42 649.0 0 42 160 20 649.0 0 20 161 31 649.0 0 31 162 102 649.0 0 102 163 110 649.0 0 110 164 106 649.0 0 106 165 73 649.0 0 73 166 55 649.0 0 55 167 140 649.0 0 140 168 171 649.0 0 171 169 136 649.0 0 136 170 108 649.0 0 108 171 48 649.0 0 48 172 62 649.0 0 62 173 47 649.0 0 47 174 38 649.0 0 38 175 55 649.0 0 55 176 36 649.0 0 36 177 40 649.0 0 40 178 66 649.0 0 66 179 135 649.0 0 135 180 67 649.0 0 67 181 142 649.0 0 142 182 41 649.0 0 41 183 67 649.0 0 67 184 182 649.0 0 182 185 240 649.0 0 240 186 176 649.0 0 176 187 124 649.0 0 124 188 75 649.0 0 75 189 57 649.0 0 57 190 47 649.0 0 47 191 44 649.0 0 44 192 47 649.0 0 47 193 43 649.0 0 43 194 45 649.0 0 45 195 43 649.0 0 43 196 35 649.0 0 35 197 40 649.0 0 40 198 66 649.0 0 66 199 158 649.0 0 158 200 88 649.0 0 88 201 107 649.0 0 107 202 140 649.0 0 140 203 105 649.0 0 105 204 270 649.0 0 270 205 229 649.0 0 229 206 283 649.0 0 283 207 188 649.0 0 188 208 73 649.0 0 73 209 111 649.0 0 111 210 101 649.0 0 101 211 94 649.0 0 94 212 77 649.0 0 77 213 59 649.0 0 59 214 47 649.0 0 47 215 71 649.0 0 71 216 86 649.0 0 86 217 64 649.0 0 64 218 57 649.0 0 57 219 60 649.0 0 60 220 86 649.0 0 86 221 83 649.0 0 83 222 133 649.0 0 133 223 148 649.0 0 148 224 1043 649.0 0 1043 225 1753 649.0 0 1753 226 1934 649.0 0 1934 227 1269 649.0 0 1269 228 478 649.0 0 478 229 58 649.0 0 58 230 39 649.0 0 39 231 64 649.0 0 64 232 58 649.0 0 58 233 51 649.0 0 51 234 114 649.0 0 114 235 96 649.0 0 96 236 121 649.0 0 121 237 75 649.0 0 75 238 59 649.0 0 59 239 207 649.0 0 207 240 103 649.0 0 103 241 199 649.0 0 199 242 45 649.0 0 45 243 51 649.0 0 51 244 44 649.0 0 44 245 59 649.0 0 59 246 43 649.0 0 43 247 36 649.0 0 36 248 67 649.0 0 67 249 40 649.0 0 40 250 33 649.0 0 33 251 18 649.0 0 18 252 31 649.0 0 31 253 81 649.0 0 81 254 78 649.0 0 78 255 100 649.0 0 100 256 29 649.0 0 29 257 99 649.0 0 99 258 67 649.0 0 67 259 184 649.0 0 184 260 67 649.0 0 67 261 98 649.0 0 98 262 51 649.0 0 51 263 70 649.0 0 70 264 134 649.0 0 134 265 117 649.0 0 117 266 189 649.0 0 189 267 35 649.0 0 35 268 48 649.0 0 48 269 62 649.0 0 62 270 49 649.0 0 49 271 51 649.0 0 51 272 58 649.0 0 58 273 59 649.0 0 59 274 84 649.0 0 84 275 72 649.0 0 72 276 44 649.0 0 44 277 32 649.0 0 32 278 47 649.0 0 47 279 49 649.0 0 49 280 138 649.0 0 138 281 67 649.0 0 67 282 110 649.0 0 110 283 35 649.0 0 35 284 23 649.0 0 23 285 37 649.0 0 37 286 61 649.0 0 61 287 40 649.0 0 40 288 35 649.0 0 35 289 49 649.0 0 49 290 44 649.0 0 44 291 53 649.0 0 53 292 64 649.0 0 64 293 63 649.0 0 63 294 84 649.0 0 84 295 113 649.0 0 113 296 82 649.0 0 82 297 103 649.0 0 103 298 82 649.0 0 82 299 141 649.0 0 141 300 56 649.0 0 56 301 230 649.0 0 230 Finished in 4.16 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 86,526 Reads with adapters: 51,382 (59.4%) Reads that were too short: 80 (0.1%) Reads written (passing filters): 51,302 (59.3%) Total basepairs processed: 23,405,657 bp Total written (filtered): 14,131,778 bp (60.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 51382 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 5 0.0 2 0 0 0 5 15 22 0.0 3 0 0 8 14 16 384 0.0 3 0 336 29 19 17 50298 0.0 3 47869 2045 290 94 18 217 0.0 3 31 125 34 27 19 108 0.0 3 7 2 64 35 20 79 0.0 3 3 0 0 76 23 2 0.0 3 0 0 0 2 24 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 26 1 0.0 3 0 1 28 4 0.0 3 0 0 0 4 30 7 0.0 3 0 0 0 7 31 2 0.0 3 0 0 0 2 33 2 0.0 3 0 0 0 2 35 35 0.0 3 1 0 3 31 36 2 0.0 3 0 0 0 2 38 1 0.0 3 0 0 0 1 40 1 0.0 3 1 41 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 52 5 0.0 3 0 0 0 5 53 2 0.0 3 0 0 0 2 54 12 0.0 3 0 0 0 12 55 2 0.0 3 0 0 0 2 56 6 0.0 3 0 0 0 6 57 2 0.0 3 0 0 0 2 58 2 0.0 3 0 0 0 2 60 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 65 4 0.0 3 0 0 1 3 68 16 0.0 3 0 0 0 16 69 2 0.0 3 0 0 0 2 76 1 0.0 3 0 0 1 78 10 0.0 3 0 0 0 10 79 5 0.0 3 0 0 0 5 80 1 0.0 3 0 0 0 1 81 3 0.0 3 0 0 0 3 82 1 0.0 3 0 0 0 1 86 2 0.0 3 0 0 0 2 87 2 0.0 3 0 0 0 2 90 4 0.0 3 0 0 0 4 91 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 1 97 2 0.0 3 0 0 0 2 98 2 0.0 3 0 0 0 2 99 5 0.0 3 0 0 1 4 100 1 0.0 3 0 0 1 105 1 0.0 3 0 0 0 1 106 2 0.0 3 0 0 1 1 108 2 0.0 3 0 0 1 1 111 1 0.0 3 0 0 0 1 112 3 0.0 3 0 0 0 3 113 13 0.0 3 0 0 2 11 114 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 117 3 0.0 3 0 0 0 3 121 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 126 3 0.0 3 0 0 0 3 127 2 0.0 3 0 0 1 1 128 2 0.0 3 0 0 0 2 129 1 0.0 3 0 0 0 1 130 2 0.0 3 0 0 0 2 131 1 0.0 3 0 0 0 1 132 2 0.0 3 0 0 0 2 134 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 145 2 0.0 3 0 0 0 2 146 5 0.0 3 0 0 0 5 147 3 0.0 3 0 0 1 2 148 1 0.0 3 0 0 1 149 1 0.0 3 1 150 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 154 3 0.0 3 0 0 0 3 155 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 1 162 1 0.0 3 0 0 0 1 163 4 0.0 3 0 0 0 4 167 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 176 2 0.0 3 0 0 0 2 177 1 0.0 3 0 0 0 1 179 3 0.0 3 0 0 1 2 180 2 0.0 3 0 0 0 2 183 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 201 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 205 2 0.0 3 0 0 0 2 207 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 216 2 0.0 3 0 0 1 1 223 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 1 238 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 245 1 0.0 3 0 0 0 1 247 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 0 1 249 1 0.0 3 0 0 0 1 264 2 0.0 3 0 0 0 2 266 2 0.0 3 0 0 0 2 281 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.82 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 51,302 Reads with adapters: 934 (1.8%) Reads written (passing filters): 51,302 (100.0%) Total basepairs processed: 14,131,778 bp Total written (filtered): 14,128,889 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 934 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 38.2% C: 20.0% G: 28.2% T: 13.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 878 801.6 0 878 4 40 200.4 0 40 5 12 50.1 0 12 7 3 3.1 0 3 14 1 0.0 1 0 1