This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.52 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 94,158 (3.5%) Reads that were too short: 10,477 (0.4%) Reads written (passing filters): 83,681 (3.1%) Total basepairs processed: 800,111,879 bp Total written (filtered): 22,983,782 bp (2.9%) === Adapter 1 === Sequence: AATAGG; Type: regular 5'; Length: 6; Trimmed: 94158 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 329 649.0 0 329 7 865 649.0 0 865 8 50789 649.0 0 50789 9 17502 649.0 0 17502 10 29 649.0 0 29 11 15 649.0 0 15 12 10 649.0 0 10 13 12 649.0 0 12 14 10 649.0 0 10 15 14 649.0 0 14 16 15 649.0 0 15 17 18 649.0 0 18 18 27 649.0 0 27 19 23 649.0 0 23 20 19 649.0 0 19 21 9 649.0 0 9 22 7 649.0 0 7 23 15 649.0 0 15 24 17 649.0 0 17 25 5 649.0 0 5 26 9 649.0 0 9 27 8 649.0 0 8 28 18 649.0 0 18 29 32 649.0 0 32 30 50 649.0 0 50 31 17 649.0 0 17 32 16 649.0 0 16 33 12 649.0 0 12 34 15 649.0 0 15 35 22 649.0 0 22 36 14 649.0 0 14 37 24 649.0 0 24 38 11 649.0 0 11 39 17 649.0 0 17 40 5 649.0 0 5 41 12 649.0 0 12 42 28 649.0 0 28 43 48 649.0 0 48 44 92 649.0 0 92 45 312 649.0 0 312 46 141 649.0 0 141 47 225 649.0 0 225 48 14 649.0 0 14 49 8 649.0 0 8 50 17 649.0 0 17 51 13 649.0 0 13 52 11 649.0 0 11 53 10 649.0 0 10 54 14 649.0 0 14 55 12 649.0 0 12 56 13 649.0 0 13 57 10 649.0 0 10 58 19 649.0 0 19 59 12 649.0 0 12 60 15 649.0 0 15 61 14 649.0 0 14 62 13 649.0 0 13 63 11 649.0 0 11 64 9 649.0 0 9 65 11 649.0 0 11 66 10 649.0 0 10 67 14 649.0 0 14 68 11 649.0 0 11 69 20 649.0 0 20 70 39 649.0 0 39 71 102 649.0 0 102 72 48 649.0 0 48 73 92 649.0 0 92 74 11 649.0 0 11 75 16 649.0 0 16 76 13 649.0 0 13 77 27 649.0 0 27 78 37 649.0 0 37 79 75 649.0 0 75 80 42 649.0 0 42 81 33 649.0 0 33 82 29 649.0 0 29 83 56 649.0 0 56 84 70 649.0 0 70 85 55 649.0 0 55 86 71 649.0 0 71 87 36 649.0 0 36 88 55 649.0 0 55 89 61 649.0 0 61 90 81 649.0 0 81 91 71 649.0 0 71 92 92 649.0 0 92 93 79 649.0 0 79 94 1112 649.0 0 1112 95 690 649.0 0 690 96 1301 649.0 0 1301 97 327 649.0 0 327 98 49 649.0 0 49 99 49 649.0 0 49 100 31 649.0 0 31 101 54 649.0 0 54 102 28 649.0 0 28 103 28 649.0 0 28 104 25 649.0 0 25 105 29 649.0 0 29 106 62 649.0 0 62 107 35 649.0 0 35 108 32 649.0 0 32 109 22 649.0 0 22 110 24 649.0 0 24 111 36 649.0 0 36 112 50 649.0 0 50 113 222 649.0 0 222 114 417 649.0 0 417 115 280 649.0 0 280 116 240 649.0 0 240 117 59 649.0 0 59 118 32 649.0 0 32 119 48 649.0 0 48 120 53 649.0 0 53 121 26 649.0 0 26 122 51 649.0 0 51 123 46 649.0 0 46 124 38 649.0 0 38 125 37 649.0 0 37 126 41 649.0 0 41 127 39 649.0 0 39 128 82 649.0 0 82 129 63 649.0 0 63 130 51 649.0 0 51 131 61 649.0 0 61 132 61 649.0 0 61 133 109 649.0 0 109 134 107 649.0 0 107 135 417 649.0 0 417 136 464 649.0 0 464 137 350 649.0 0 350 138 301 649.0 0 301 139 172 649.0 0 172 140 139 649.0 0 139 141 65 649.0 0 65 142 76 649.0 0 76 143 73 649.0 0 73 144 65 649.0 0 65 145 46 649.0 0 46 146 37 649.0 0 37 147 28 649.0 0 28 148 37 649.0 0 37 149 36 649.0 0 36 150 38 649.0 0 38 151 46 649.0 0 46 152 33 649.0 0 33 153 35 649.0 0 35 154 33 649.0 0 33 155 57 649.0 0 57 156 53 649.0 0 53 157 48 649.0 0 48 158 40 649.0 0 40 159 23 649.0 0 23 160 27 649.0 0 27 161 22 649.0 0 22 162 66 649.0 0 66 163 69 649.0 0 69 164 81 649.0 0 81 165 50 649.0 0 50 166 39 649.0 0 39 167 99 649.0 0 99 168 135 649.0 0 135 169 87 649.0 0 87 170 75 649.0 0 75 171 33 649.0 0 33 172 40 649.0 0 40 173 30 649.0 0 30 174 41 649.0 0 41 175 41 649.0 0 41 176 24 649.0 0 24 177 25 649.0 0 25 178 53 649.0 0 53 179 83 649.0 0 83 180 54 649.0 0 54 181 83 649.0 0 83 182 27 649.0 0 27 183 42 649.0 0 42 184 94 649.0 0 94 185 117 649.0 0 117 186 84 649.0 0 84 187 74 649.0 0 74 188 52 649.0 0 52 189 56 649.0 0 56 190 50 649.0 0 50 191 49 649.0 0 49 192 50 649.0 0 50 193 33 649.0 0 33 194 55 649.0 0 55 195 39 649.0 0 39 196 37 649.0 0 37 197 48 649.0 0 48 198 42 649.0 0 42 199 69 649.0 0 69 200 46 649.0 0 46 201 71 649.0 0 71 202 92 649.0 0 92 203 60 649.0 0 60 204 150 649.0 0 150 205 151 649.0 0 151 206 145 649.0 0 145 207 119 649.0 0 119 208 56 649.0 0 56 209 50 649.0 0 50 210 75 649.0 0 75 211 57 649.0 0 57 212 48 649.0 0 48 213 32 649.0 0 32 214 42 649.0 0 42 215 69 649.0 0 69 216 66 649.0 0 66 217 40 649.0 0 40 218 53 649.0 0 53 219 39 649.0 0 39 220 54 649.0 0 54 221 58 649.0 0 58 222 101 649.0 0 101 223 97 649.0 0 97 224 1147 649.0 0 1147 225 1352 649.0 0 1352 226 1679 649.0 0 1679 227 1017 649.0 0 1017 228 371 649.0 0 371 229 45 649.0 0 45 230 39 649.0 0 39 231 50 649.0 0 50 232 50 649.0 0 50 233 44 649.0 0 44 234 49 649.0 0 49 235 54 649.0 0 54 236 83 649.0 0 83 237 68 649.0 0 68 238 29 649.0 0 29 239 101 649.0 0 101 240 42 649.0 0 42 241 66 649.0 0 66 242 27 649.0 0 27 243 24 649.0 0 24 244 40 649.0 0 40 245 36 649.0 0 36 246 46 649.0 0 46 247 27 649.0 0 27 248 42 649.0 0 42 249 24 649.0 0 24 250 33 649.0 0 33 251 28 649.0 0 28 252 27 649.0 0 27 253 64 649.0 0 64 254 53 649.0 0 53 255 60 649.0 0 60 256 24 649.0 0 24 257 52 649.0 0 52 258 42 649.0 0 42 259 84 649.0 0 84 260 61 649.0 0 61 261 47 649.0 0 47 262 32 649.0 0 32 263 36 649.0 0 36 264 68 649.0 0 68 265 37 649.0 0 37 266 95 649.0 0 95 267 34 649.0 0 34 268 49 649.0 0 49 269 53 649.0 0 53 270 26 649.0 0 26 271 15 649.0 0 15 272 38 649.0 0 38 273 32 649.0 0 32 274 31 649.0 0 31 275 39 649.0 0 39 276 30 649.0 0 30 277 25 649.0 0 25 278 30 649.0 0 30 279 33 649.0 0 33 280 74 649.0 0 74 281 40 649.0 0 40 282 58 649.0 0 58 283 31 649.0 0 31 284 22 649.0 0 22 285 28 649.0 0 28 286 30 649.0 0 30 287 31 649.0 0 31 288 28 649.0 0 28 289 34 649.0 0 34 290 37 649.0 0 37 291 23 649.0 0 23 292 32 649.0 0 32 293 45 649.0 0 45 294 56 649.0 0 56 295 68 649.0 0 68 296 42 649.0 0 42 297 45 649.0 0 45 298 46 649.0 0 46 299 60 649.0 0 60 300 38 649.0 0 38 301 125 649.0 0 125 Finished in 4.42 s (53 us/read; 1.14 M reads/minute). === Summary === Total reads processed: 83,681 Reads with adapters: 18,916 (22.6%) Reads that were too short: 67 (0.1%) Reads written (passing filters): 18,849 (22.5%) Total basepairs processed: 22,983,782 bp Total written (filtered): 5,107,392 bp (22.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 18916 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 6 0.0 3 0 0 6 19 187 0.0 3 0 186 1 20 18382 0.0 4 18008 353 17 1 3 21 31 0.0 4 8 23 22 9 0.0 4 1 0 7 0 1 23 18 0.0 4 0 0 1 14 3 24 1 0.0 4 0 0 0 0 1 27 23 0.0 4 0 0 0 0 23 32 1 0.0 4 1 34 1 0.0 4 1 35 2 0.0 4 1 0 0 0 1 36 1 0.0 4 0 0 0 0 1 39 1 0.0 4 0 0 0 0 1 42 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 47 1 0.0 4 0 0 0 0 1 59 2 0.0 4 0 0 0 0 2 61 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 89 22 0.0 4 0 0 0 0 22 90 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 100 1 0.0 4 1 107 1 0.0 4 0 0 0 0 1 109 1 0.0 4 1 111 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 1 118 1 0.0 4 0 0 0 0 1 119 1 0.0 4 0 0 0 0 1 121 3 0.0 4 2 1 124 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 109 0.0 4 0 0 0 0 109 128 1 0.0 4 1 131 1 0.0 4 0 0 0 0 1 136 1 0.0 4 1 145 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 149 1 0.0 4 1 151 1 0.0 4 1 157 2 0.0 4 0 1 0 0 1 158 2 0.0 4 2 160 1 0.0 4 0 1 172 27 0.0 4 0 0 0 0 27 177 1 0.0 4 0 0 0 0 1 185 1 0.0 4 1 192 1 0.0 4 0 0 0 0 1 195 1 0.0 4 1 197 2 0.0 4 0 1 0 0 1 201 2 0.0 4 0 1 0 1 209 1 0.0 4 1 218 3 0.0 4 1 1 0 0 1 219 4 0.0 4 3 1 220 1 0.0 4 1 221 2 0.0 4 1 1 226 2 0.0 4 1 1 227 1 0.0 4 1 228 4 0.0 4 1 0 0 0 3 230 1 0.0 4 0 0 0 0 1 232 3 0.0 4 0 1 0 0 2 233 2 0.0 4 0 0 0 0 2 235 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 239 1 0.0 4 0 1 241 2 0.0 4 0 0 0 0 2 244 1 0.0 4 1 251 2 0.0 4 1 1 258 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 272 1 0.0 4 0 0 0 0 1 274 1 0.0 4 0 0 0 1 275 4 0.0 4 1 0 0 0 3 277 1 0.0 4 1 285 1 0.0 4 0 0 0 0 1 286 1 0.0 4 0 1 288 1 0.0 4 0 0 0 0 1 292 1 0.0 4 1 293 3 0.0 4 1 0 0 1 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.66 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 18,849 Reads with adapters: 2,456 (13.0%) Reads written (passing filters): 18,849 (100.0%) Total basepairs processed: 5,107,392 bp Total written (filtered): 5,077,739 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2456 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.4% C: 3.3% G: 91.8% T: 2.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 4 294.5 0 4 4 4 73.6 0 4 9 984 0.1 0 31 953 10 1207 0.0 1 50 1157 11 174 0.0 1 1 173 12 1 0.0 1 0 1 13 17 0.0 1 9 8 18 14 0.0 1 14 19 3 0.0 1 2 1 22 5 0.0 1 3 2 33 1 0.0 1 1 42 1 0.0 1 1 66 1 0.0 1 1 80 5 0.0 1 5 89 1 0.0 1 0 1 104 1 0.0 1 0 1 106 1 0.0 1 1 114 4 0.0 1 4 122 6 0.0 1 5 1 145 2 0.0 1 1 1 150 4 0.0 1 2 2 151 1 0.0 1 0 1 152 1 0.0 1 0 1 156 2 0.0 1 2 159 1 0.0 1 1 165 1 0.0 1 1 178 1 0.0 1 1 180 1 0.0 1 1 219 1 0.0 1 1 223 3 0.0 1 2 1 224 1 0.0 1 1 266 1 0.0 1 1 270 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.