This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGAAGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.59 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 480,476 (18.1%) Reads that were too short: 25,106 (0.9%) Reads written (passing filters): 455,370 (17.1%) Total basepairs processed: 800,111,879 bp Total written (filtered): 85,853,896 bp (10.7%) === Adapter 1 === Sequence: AGAAGA; Type: regular 5'; Length: 6; Trimmed: 480476 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1463 649.0 0 1463 7 41749 649.0 0 41749 8 35280 649.0 0 35280 9 147 649.0 0 147 10 66 649.0 0 66 11 102 649.0 0 102 12 75 649.0 0 75 13 56 649.0 0 56 14 63 649.0 0 63 15 66 649.0 0 66 16 64 649.0 0 64 17 54 649.0 0 54 18 57 649.0 0 57 19 63 649.0 0 63 20 51 649.0 0 51 21 68 649.0 0 68 22 67 649.0 0 67 23 82 649.0 0 82 24 80 649.0 0 80 25 222 649.0 0 222 26 166 649.0 0 166 27 316 649.0 0 316 28 230 649.0 0 230 29 135 649.0 0 135 30 120 649.0 0 120 31 65 649.0 0 65 32 65 649.0 0 65 33 75 649.0 0 75 34 84 649.0 0 84 35 111 649.0 0 111 36 182 649.0 0 182 37 267 649.0 0 267 38 100 649.0 0 100 39 71 649.0 0 71 40 131 649.0 0 131 41 87 649.0 0 87 42 109 649.0 0 109 43 131 649.0 0 131 44 72 649.0 0 72 45 79 649.0 0 79 46 117 649.0 0 117 47 66 649.0 0 66 48 62 649.0 0 62 49 75 649.0 0 75 50 74 649.0 0 74 51 90 649.0 0 90 52 93 649.0 0 93 53 103 649.0 0 103 54 94 649.0 0 94 55 60 649.0 0 60 56 77 649.0 0 77 57 53 649.0 0 53 58 71 649.0 0 71 59 79 649.0 0 79 60 89 649.0 0 89 61 113 649.0 0 113 62 187 649.0 0 187 63 223 649.0 0 223 64 101 649.0 0 101 65 83 649.0 0 83 66 97 649.0 0 97 67 115 649.0 0 115 68 153 649.0 0 153 69 160 649.0 0 160 70 169 649.0 0 169 71 183 649.0 0 183 72 846 649.0 0 846 73 403 649.0 0 403 74 1260 649.0 0 1260 75 2224 649.0 0 2224 76 1768 649.0 0 1768 77 604 649.0 0 604 78 333 649.0 0 333 79 1093 649.0 0 1093 80 491 649.0 0 491 81 1676 649.0 0 1676 82 971 649.0 0 971 83 1848 649.0 0 1848 84 441 649.0 0 441 85 394 649.0 0 394 86 467 649.0 0 467 87 127 649.0 0 127 88 138 649.0 0 138 89 293 649.0 0 293 90 142 649.0 0 142 91 124 649.0 0 124 92 401 649.0 0 401 93 167 649.0 0 167 94 161 649.0 0 161 95 304 649.0 0 304 96 234 649.0 0 234 97 175 649.0 0 175 98 222 649.0 0 222 99 223 649.0 0 223 100 172 649.0 0 172 101 233 649.0 0 233 102 289 649.0 0 289 103 341 649.0 0 341 104 422 649.0 0 422 105 668 649.0 0 668 106 368 649.0 0 368 107 360 649.0 0 360 108 656 649.0 0 656 109 374 649.0 0 374 110 455 649.0 0 455 111 627 649.0 0 627 112 686 649.0 0 686 113 920 649.0 0 920 114 725 649.0 0 725 115 1001 649.0 0 1001 116 1259 649.0 0 1259 117 1861 649.0 0 1861 118 26085 649.0 0 26085 119 17435 649.0 0 17435 120 36365 649.0 0 36365 121 2486 649.0 0 2486 122 1842 649.0 0 1842 123 4655 649.0 0 4655 124 472 649.0 0 472 125 423 649.0 0 423 126 733 649.0 0 733 127 502 649.0 0 502 128 593 649.0 0 593 129 573 649.0 0 573 130 488 649.0 0 488 131 685 649.0 0 685 132 1097 649.0 0 1097 133 815 649.0 0 815 134 883 649.0 0 883 135 1077 649.0 0 1077 136 1182 649.0 0 1182 137 1343 649.0 0 1343 138 2279 649.0 0 2279 139 31824 649.0 0 31824 140 13877 649.0 0 13877 141 30670 649.0 0 30670 142 8109 649.0 0 8109 143 2854 649.0 0 2854 144 2974 649.0 0 2974 145 32902 649.0 0 32902 146 26150 649.0 0 26150 147 34368 649.0 0 34368 148 8388 649.0 0 8388 149 6155 649.0 0 6155 150 1082 649.0 0 1082 151 918 649.0 0 918 152 531 649.0 0 531 153 340 649.0 0 340 154 475 649.0 0 475 155 798 649.0 0 798 156 461 649.0 0 461 157 1770 649.0 0 1770 158 1563 649.0 0 1563 159 764 649.0 0 764 160 426 649.0 0 426 161 419 649.0 0 419 162 471 649.0 0 471 163 625 649.0 0 625 164 1245 649.0 0 1245 165 9519 649.0 0 9519 166 6720 649.0 0 6720 167 4305 649.0 0 4305 168 559 649.0 0 559 169 292 649.0 0 292 170 238 649.0 0 238 171 226 649.0 0 226 172 261 649.0 0 261 173 219 649.0 0 219 174 778 649.0 0 778 175 444 649.0 0 444 176 219 649.0 0 219 177 152 649.0 0 152 178 146 649.0 0 146 179 141 649.0 0 141 180 168 649.0 0 168 181 182 649.0 0 182 182 201 649.0 0 201 183 162 649.0 0 162 184 152 649.0 0 152 185 161 649.0 0 161 186 140 649.0 0 140 187 172 649.0 0 172 188 163 649.0 0 163 189 170 649.0 0 170 190 170 649.0 0 170 191 168 649.0 0 168 192 304 649.0 0 304 193 291 649.0 0 291 194 330 649.0 0 330 195 237 649.0 0 237 196 184 649.0 0 184 197 157 649.0 0 157 198 142 649.0 0 142 199 482 649.0 0 482 200 398 649.0 0 398 201 240 649.0 0 240 202 181 649.0 0 181 203 142 649.0 0 142 204 152 649.0 0 152 205 195 649.0 0 195 206 184 649.0 0 184 207 163 649.0 0 163 208 178 649.0 0 178 209 214 649.0 0 214 210 219 649.0 0 219 211 192 649.0 0 192 212 137 649.0 0 137 213 165 649.0 0 165 214 136 649.0 0 136 215 176 649.0 0 176 216 324 649.0 0 324 217 843 649.0 0 843 218 749 649.0 0 749 219 400 649.0 0 400 220 160 649.0 0 160 221 129 649.0 0 129 222 97 649.0 0 97 223 149 649.0 0 149 224 126 649.0 0 126 225 175 649.0 0 175 226 196 649.0 0 196 227 268 649.0 0 268 228 195 649.0 0 195 229 165 649.0 0 165 230 145 649.0 0 145 231 136 649.0 0 136 232 138 649.0 0 138 233 132 649.0 0 132 234 126 649.0 0 126 235 123 649.0 0 123 236 127 649.0 0 127 237 165 649.0 0 165 238 142 649.0 0 142 239 171 649.0 0 171 240 142 649.0 0 142 241 144 649.0 0 144 242 140 649.0 0 140 243 155 649.0 0 155 244 133 649.0 0 133 245 152 649.0 0 152 246 296 649.0 0 296 247 216 649.0 0 216 248 317 649.0 0 317 249 179 649.0 0 179 250 107 649.0 0 107 251 137 649.0 0 137 252 125 649.0 0 125 253 119 649.0 0 119 254 114 649.0 0 114 255 158 649.0 0 158 256 156 649.0 0 156 257 168 649.0 0 168 258 122 649.0 0 122 259 142 649.0 0 142 260 173 649.0 0 173 261 219 649.0 0 219 262 329 649.0 0 329 263 217 649.0 0 217 264 306 649.0 0 306 265 215 649.0 0 215 266 173 649.0 0 173 267 190 649.0 0 190 268 166 649.0 0 166 269 128 649.0 0 128 270 114 649.0 0 114 271 143 649.0 0 143 272 167 649.0 0 167 273 154 649.0 0 154 274 181 649.0 0 181 275 180 649.0 0 180 276 155 649.0 0 155 277 165 649.0 0 165 278 199 649.0 0 199 279 199 649.0 0 199 280 215 649.0 0 215 281 165 649.0 0 165 282 158 649.0 0 158 283 143 649.0 0 143 284 149 649.0 0 149 285 140 649.0 0 140 286 127 649.0 0 127 287 193 649.0 0 193 288 231 649.0 0 231 289 190 649.0 0 190 290 195 649.0 0 195 291 180 649.0 0 180 292 160 649.0 0 160 293 188 649.0 0 188 294 172 649.0 0 172 295 184 649.0 0 184 296 240 649.0 0 240 297 265 649.0 0 265 298 303 649.0 0 303 299 392 649.0 0 392 300 483 649.0 0 483 301 5823 649.0 0 5823 Finished in 16.17 s (36 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 455,370 Reads with adapters: 59,220 (13.0%) Reads that were too short: 1,269 (0.3%) Reads written (passing filters): 57,951 (12.7%) Total basepairs processed: 85,853,896 bp Total written (filtered): 13,350,880 bp (15.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 59220 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 7 0.0 2 0 0 0 7 15 22 0.0 3 0 0 13 9 16 342 0.0 3 0 280 40 22 17 40737 0.0 3 38399 1956 305 77 18 127 0.0 3 18 75 9 25 19 144 0.0 3 6 4 79 55 20 52 0.0 3 0 0 0 52 21 2 0.0 3 1 0 0 1 22 3 0.0 3 0 1 0 2 23 36 0.0 3 0 0 0 36 24 3 0.0 3 0 0 0 3 25 3 0.0 3 0 0 0 3 26 3436 0.0 3 0 0 26 3410 27 32 0.0 3 0 0 1 31 28 7 0.0 3 0 0 1 6 29 295 0.0 3 0 0 12 283 30 11 0.0 3 0 0 0 11 31 4 0.0 3 2 0 0 2 32 6 0.0 3 0 0 0 6 33 2 0.0 3 0 0 0 2 34 1 0.0 3 0 0 0 1 36 1 0.0 3 1 39 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 42 11 0.0 3 0 0 0 11 43 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 46 10 0.0 3 0 0 1 9 47 18 0.0 3 0 0 1 17 48 11 0.0 3 0 0 0 11 49 8 0.0 3 0 0 0 8 50 123 0.0 3 0 0 4 119 51 10 0.0 3 0 0 0 10 52 170 0.0 3 0 0 1 169 53 732 0.0 3 0 0 3 729 54 15 0.0 3 0 0 0 15 55 5 0.0 3 0 0 3 2 56 5 0.0 3 0 0 0 5 57 1 0.0 3 0 0 0 1 58 3 0.0 3 0 0 0 3 59 4 0.0 3 0 0 0 4 60 2 0.0 3 0 0 0 2 62 4 0.0 3 1 0 0 3 63 1 0.0 3 0 0 0 1 64 3 0.0 3 0 0 0 3 65 1 0.0 3 0 0 0 1 66 3 0.0 3 0 0 0 3 67 2 0.0 3 0 0 0 2 68 2 0.0 3 0 0 0 2 69 4 0.0 3 0 0 1 3 70 12 0.0 3 0 0 0 12 71 19 0.0 3 0 0 0 19 72 10277 0.0 3 1 0 131 10145 73 380 0.0 3 0 0 10 370 74 602 0.0 3 0 0 3 599 75 2 0.0 3 0 0 0 2 76 55 0.0 3 0 0 2 53 77 149 0.0 3 0 0 5 144 78 35 0.0 3 0 0 0 35 79 11 0.0 3 1 0 0 10 80 2 0.0 3 0 0 1 1 81 1 0.0 3 0 0 1 82 6 0.0 3 1 0 0 5 84 1 0.0 3 0 0 0 1 85 17 0.0 3 0 0 0 17 86 25 0.0 3 0 0 0 25 87 42 0.0 3 0 0 1 41 88 16 0.0 3 0 0 1 15 89 8 0.0 3 1 0 0 7 90 5 0.0 3 0 0 0 5 93 2 0.0 3 0 0 0 2 94 1 0.0 3 0 0 0 1 95 3 0.0 3 0 0 0 3 96 4 0.0 3 0 0 0 4 97 6 0.0 3 0 0 0 6 98 6 0.0 3 0 0 0 6 99 7 0.0 3 0 0 0 7 100 2 0.0 3 0 0 0 2 101 6 0.0 3 0 0 0 6 102 1 0.0 3 0 0 0 1 103 2 0.0 3 0 0 0 2 104 1 0.0 3 0 0 0 1 105 3 0.0 3 0 0 0 3 106 8 0.0 3 0 0 1 7 107 4 0.0 3 0 0 0 4 108 3 0.0 3 0 0 0 3 109 18 0.0 3 0 0 0 18 110 13 0.0 3 0 0 0 13 111 21 0.0 3 0 0 0 21 112 4 0.0 3 0 0 0 4 113 5 0.0 3 0 0 0 5 114 12 0.0 3 0 0 0 12 115 1 0.0 3 0 0 0 1 116 2 0.0 3 0 0 0 2 117 2 0.0 3 0 0 0 2 119 2 0.0 3 0 0 0 2 121 2 0.0 3 0 0 0 2 122 1 0.0 3 0 0 0 1 123 4 0.0 3 0 0 0 4 124 8 0.0 3 0 0 0 8 125 16 0.0 3 0 0 0 16 126 5 0.0 3 0 0 0 5 127 26 0.0 3 0 0 1 25 128 62 0.0 3 0 0 3 59 129 28 0.0 3 0 0 0 28 130 7 0.0 3 0 0 0 7 131 5 0.0 3 0 0 0 5 132 6 0.0 3 0 0 0 6 133 54 0.0 3 0 0 0 54 134 13 0.0 3 0 0 1 12 135 6 0.0 3 0 0 2 4 136 6 0.0 3 0 0 0 6 137 7 0.0 3 0 0 1 6 138 215 0.0 3 0 0 6 209 139 39 0.0 3 0 0 0 39 140 25 0.0 3 0 0 1 24 141 117 0.0 3 0 0 3 114 142 27 0.0 3 0 0 1 26 143 3 0.0 3 0 0 0 3 144 3 0.0 3 0 0 0 3 145 4 0.0 3 0 0 0 4 146 21 0.0 3 0 0 1 20 147 22 0.0 3 0 0 1 21 148 9 0.0 3 0 0 0 9 149 5 0.0 3 0 0 0 5 150 8 0.0 3 0 0 0 8 151 10 0.0 3 0 0 0 10 152 4 0.0 3 0 0 0 4 153 3 0.0 3 0 0 0 3 154 1 0.0 3 0 0 0 1 155 5 0.0 3 0 0 0 5 156 54 0.0 3 0 0 0 54 157 1 0.0 3 0 0 0 1 158 5 0.0 3 0 0 0 5 159 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 161 3 0.0 3 0 0 0 3 162 2 0.0 3 0 0 0 2 163 2 0.0 3 0 0 0 2 164 5 0.0 3 0 0 0 5 165 6 0.0 3 0 0 1 5 166 8 0.0 3 0 0 0 8 167 4 0.0 3 0 0 0 4 168 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 172 3 0.0 3 0 0 0 3 173 3 0.0 3 0 0 0 3 174 19 0.0 3 0 0 0 19 175 7 0.0 3 0 0 0 7 176 7 0.0 3 0 0 0 7 177 4 0.0 3 0 0 0 4 179 1 0.0 3 0 0 0 1 180 6 0.0 3 0 0 0 6 183 3 0.0 3 0 0 0 3 192 2 0.0 3 0 0 1 1 193 1 0.0 3 0 0 0 1 194 2 0.0 3 0 0 0 2 196 4 0.0 3 0 0 0 4 199 4 0.0 3 0 0 0 4 202 2 0.0 3 0 0 0 2 205 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 208 2 0.0 3 0 0 0 2 209 1 0.0 3 0 0 0 1 211 2 0.0 3 0 0 1 1 212 2 0.0 3 0 0 0 2 213 2 0.0 3 0 0 0 2 216 2 0.0 3 0 0 0 2 217 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 226 1 0.0 3 0 0 0 1 230 4 0.0 3 0 0 0 4 231 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 257 1 0.0 3 0 0 0 1 261 1 0.0 3 0 0 0 1 267 1 0.0 3 0 0 0 1 268 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 280 7 0.0 3 0 0 0 7 281 4 0.0 3 0 0 0 4 282 2 0.0 3 0 0 0 2 283 1 0.0 3 0 0 0 1 287 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.85 s (32 us/read; 1.88 M reads/minute). === Summary === Total reads processed: 57,951 Reads with adapters: 1,020 (1.8%) Reads written (passing filters): 57,951 (100.0%) Total basepairs processed: 13,350,880 bp Total written (filtered): 13,347,648 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1020 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 17.2% C: 32.3% G: 45.6% T: 5.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 865 905.5 0 865 4 144 226.4 0 144 5 7 56.6 0 7 6 3 14.1 0 3 8 1 0.9 0 1