This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCTGTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 70.78 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 70,103 (2.6%) Reads that were too short: 18,845 (0.7%) Reads written (passing filters): 51,258 (1.9%) Total basepairs processed: 800,111,879 bp Total written (filtered): 9,852,673 bp (1.2%) === Adapter 1 === Sequence: CCTGTC; Type: regular 5'; Length: 6; Trimmed: 70103 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 309 649.0 0 309 7 5319 649.0 0 5319 8 510 649.0 0 510 9 5978 649.0 0 5978 10 31 649.0 0 31 11 32 649.0 0 32 12 30 649.0 0 30 13 24 649.0 0 24 14 34 649.0 0 34 15 28 649.0 0 28 16 24 649.0 0 24 17 19 649.0 0 19 18 25 649.0 0 25 19 25 649.0 0 25 20 32 649.0 0 32 21 28 649.0 0 28 22 27 649.0 0 27 23 34 649.0 0 34 24 29 649.0 0 29 25 29 649.0 0 29 26 31 649.0 0 31 27 24 649.0 0 24 28 39 649.0 0 39 29 32 649.0 0 32 30 49 649.0 0 49 31 39 649.0 0 39 32 61 649.0 0 61 33 631 649.0 0 631 34 376 649.0 0 376 35 310 649.0 0 310 36 33 649.0 0 33 37 26 649.0 0 26 38 38 649.0 0 38 39 32 649.0 0 32 40 29 649.0 0 29 41 32 649.0 0 32 42 29 649.0 0 29 43 34 649.0 0 34 44 40 649.0 0 40 45 88 649.0 0 88 46 87 649.0 0 87 47 70 649.0 0 70 48 52 649.0 0 52 49 67 649.0 0 67 50 52 649.0 0 52 51 47 649.0 0 47 52 43 649.0 0 43 53 37 649.0 0 37 54 41 649.0 0 41 55 48 649.0 0 48 56 82 649.0 0 82 57 367 649.0 0 367 58 149 649.0 0 149 59 114 649.0 0 114 60 46 649.0 0 46 61 41 649.0 0 41 62 43 649.0 0 43 63 42 649.0 0 42 64 73 649.0 0 73 65 150 649.0 0 150 66 37 649.0 0 37 67 46 649.0 0 46 68 57 649.0 0 57 69 45 649.0 0 45 70 45 649.0 0 45 71 44 649.0 0 44 72 241 649.0 0 241 73 106 649.0 0 106 74 114 649.0 0 114 75 38 649.0 0 38 76 41 649.0 0 41 77 54 649.0 0 54 78 65 649.0 0 65 79 52 649.0 0 52 80 44 649.0 0 44 81 39 649.0 0 39 82 57 649.0 0 57 83 211 649.0 0 211 84 122 649.0 0 122 85 207 649.0 0 207 86 751 649.0 0 751 87 374 649.0 0 374 88 81 649.0 0 81 89 63 649.0 0 63 90 96 649.0 0 96 91 68 649.0 0 68 92 51 649.0 0 51 93 55 649.0 0 55 94 84 649.0 0 84 95 67 649.0 0 67 96 100 649.0 0 100 97 64 649.0 0 64 98 49 649.0 0 49 99 64 649.0 0 64 100 48 649.0 0 48 101 48 649.0 0 48 102 45 649.0 0 45 103 56 649.0 0 56 104 104 649.0 0 104 105 140 649.0 0 140 106 156 649.0 0 156 107 1242 649.0 0 1242 108 975 649.0 0 975 109 1810 649.0 0 1810 110 60 649.0 0 60 111 38 649.0 0 38 112 39 649.0 0 39 113 154 649.0 0 154 114 38 649.0 0 38 115 34 649.0 0 34 116 44 649.0 0 44 117 28 649.0 0 28 118 30 649.0 0 30 119 72 649.0 0 72 120 46 649.0 0 46 121 68 649.0 0 68 122 85 649.0 0 85 123 171 649.0 0 171 124 64 649.0 0 64 125 42 649.0 0 42 126 70 649.0 0 70 127 510 649.0 0 510 128 433 649.0 0 433 129 707 649.0 0 707 130 349 649.0 0 349 131 60 649.0 0 60 132 112 649.0 0 112 133 50 649.0 0 50 134 67 649.0 0 67 135 103 649.0 0 103 136 138 649.0 0 138 137 124 649.0 0 124 138 94 649.0 0 94 139 46 649.0 0 46 140 135 649.0 0 135 141 186 649.0 0 186 142 186 649.0 0 186 143 667 649.0 0 667 144 334 649.0 0 334 145 696 649.0 0 696 146 110 649.0 0 110 147 284 649.0 0 284 148 150 649.0 0 150 149 157 649.0 0 157 150 86 649.0 0 86 151 98 649.0 0 98 152 347 649.0 0 347 153 810 649.0 0 810 154 498 649.0 0 498 155 512 649.0 0 512 156 461 649.0 0 461 157 115 649.0 0 115 158 97 649.0 0 97 159 159 649.0 0 159 160 139 649.0 0 139 161 121 649.0 0 121 162 106 649.0 0 106 163 110 649.0 0 110 164 60 649.0 0 60 165 88 649.0 0 88 166 88 649.0 0 88 167 87 649.0 0 87 168 86 649.0 0 86 169 222 649.0 0 222 170 108 649.0 0 108 171 110 649.0 0 110 172 86 649.0 0 86 173 108 649.0 0 108 174 184 649.0 0 184 175 177 649.0 0 177 176 120 649.0 0 120 177 94 649.0 0 94 178 61 649.0 0 61 179 92 649.0 0 92 180 120 649.0 0 120 181 153 649.0 0 153 182 1134 649.0 0 1134 183 321 649.0 0 321 184 323 649.0 0 323 185 377 649.0 0 377 186 3683 649.0 0 3683 187 5305 649.0 0 5305 188 2066 649.0 0 2066 189 509 649.0 0 509 190 47 649.0 0 47 191 42 649.0 0 42 192 51 649.0 0 51 193 45 649.0 0 45 194 66 649.0 0 66 195 52 649.0 0 52 196 51 649.0 0 51 197 57 649.0 0 57 198 37 649.0 0 37 199 49 649.0 0 49 200 55 649.0 0 55 201 57 649.0 0 57 202 83 649.0 0 83 203 89 649.0 0 89 204 127 649.0 0 127 205 80 649.0 0 80 206 80 649.0 0 80 207 73 649.0 0 73 208 135 649.0 0 135 209 112 649.0 0 112 210 83 649.0 0 83 211 54 649.0 0 54 212 59 649.0 0 59 213 113 649.0 0 113 214 49 649.0 0 49 215 77 649.0 0 77 216 104 649.0 0 104 217 347 649.0 0 347 218 618 649.0 0 618 219 393 649.0 0 393 220 70 649.0 0 70 221 90 649.0 0 90 222 205 649.0 0 205 223 151 649.0 0 151 224 82 649.0 0 82 225 73 649.0 0 73 226 94 649.0 0 94 227 140 649.0 0 140 228 63 649.0 0 63 229 77 649.0 0 77 230 59 649.0 0 59 231 68 649.0 0 68 232 91 649.0 0 91 233 102 649.0 0 102 234 94 649.0 0 94 235 100 649.0 0 100 236 97 649.0 0 97 237 57 649.0 0 57 238 63 649.0 0 63 239 81 649.0 0 81 240 69 649.0 0 69 241 132 649.0 0 132 242 164 649.0 0 164 243 253 649.0 0 253 244 131 649.0 0 131 245 182 649.0 0 182 246 99 649.0 0 99 247 81 649.0 0 81 248 94 649.0 0 94 249 77 649.0 0 77 250 464 649.0 0 464 251 225 649.0 0 225 252 541 649.0 0 541 253 171 649.0 0 171 254 252 649.0 0 252 255 108 649.0 0 108 256 103 649.0 0 103 257 84 649.0 0 84 258 79 649.0 0 79 259 102 649.0 0 102 260 150 649.0 0 150 261 459 649.0 0 459 262 350 649.0 0 350 263 155 649.0 0 155 264 203 649.0 0 203 265 73 649.0 0 73 266 86 649.0 0 86 267 106 649.0 0 106 268 93 649.0 0 93 269 133 649.0 0 133 270 181 649.0 0 181 271 228 649.0 0 228 272 316 649.0 0 316 273 207 649.0 0 207 274 96 649.0 0 96 275 82 649.0 0 82 276 95 649.0 0 95 277 106 649.0 0 106 278 208 649.0 0 208 279 215 649.0 0 215 280 594 649.0 0 594 281 554 649.0 0 554 282 584 649.0 0 584 283 142 649.0 0 142 284 86 649.0 0 86 285 128 649.0 0 128 286 132 649.0 0 132 287 97 649.0 0 97 288 179 649.0 0 179 289 215 649.0 0 215 290 225 649.0 0 225 291 334 649.0 0 334 292 116 649.0 0 116 293 132 649.0 0 132 294 220 649.0 0 220 295 258 649.0 0 258 296 589 649.0 0 589 297 1159 649.0 0 1159 298 682 649.0 0 682 299 610 649.0 0 610 300 193 649.0 0 193 301 130 649.0 0 130 Finished in 2.01 s (39 us/read; 1.53 M reads/minute). === Summary === Total reads processed: 51,258 Reads with adapters: 7,010 (13.7%) Reads that were too short: 144 (0.3%) Reads written (passing filters): 6,866 (13.4%) Total basepairs processed: 9,852,673 bp Total written (filtered): 1,839,466 bp (18.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 7010 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 7 0.0 3 0 0 1 6 16 59 0.0 3 0 53 5 1 17 6437 0.0 3 6024 332 59 22 18 11 0.0 3 0 4 2 5 19 14 0.0 3 0 0 9 5 20 20 0.0 3 0 0 1 19 24 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 26 1 0.0 3 0 0 0 1 27 8 0.0 3 0 0 1 7 30 2 0.0 3 0 0 0 2 31 17 0.0 3 0 0 0 17 32 1 0.0 3 0 0 0 1 33 3 0.0 3 0 0 0 3 34 2 0.0 3 0 0 0 2 37 3 0.0 3 0 0 0 3 38 3 0.0 3 0 0 0 3 40 1 0.0 3 0 0 0 1 41 3 0.0 3 0 0 0 3 43 39 0.0 3 0 0 2 37 44 2 0.0 3 0 0 1 1 45 1 0.0 3 0 0 0 1 47 2 0.0 3 0 0 0 2 48 16 0.0 3 0 0 0 16 49 2 0.0 3 0 0 0 2 50 1 0.0 3 0 0 0 1 51 3 0.0 3 0 0 0 3 52 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 56 2 0.0 3 0 0 0 2 57 6 0.0 3 0 0 0 6 58 2 0.0 3 0 0 0 2 59 47 0.0 3 0 0 0 47 60 4 0.0 3 0 0 0 4 61 26 0.0 3 1 0 0 25 62 9 0.0 3 0 0 0 9 63 1 0.0 3 0 0 0 1 64 17 0.0 3 0 0 2 15 65 2 0.0 3 0 0 0 2 66 4 0.0 3 0 0 0 4 67 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 76 2 0.0 3 0 0 0 2 78 1 0.0 3 0 0 0 1 81 2 0.0 3 0 0 0 2 82 1 0.0 3 0 0 0 1 83 2 0.0 3 0 0 0 2 84 4 0.0 3 0 0 0 4 85 26 0.0 3 0 0 1 25 86 1 0.0 3 0 0 0 1 87 13 0.0 3 0 0 0 13 88 2 0.0 3 0 0 0 2 89 2 0.0 3 0 0 0 2 90 1 0.0 3 0 0 0 1 91 2 0.0 3 0 0 0 2 98 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 102 2 0.0 3 0 0 0 2 104 1 0.0 3 0 0 0 1 107 2 0.0 3 0 0 0 2 108 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 110 6 0.0 3 0 0 1 5 111 3 0.0 3 0 0 0 3 112 1 0.0 3 0 0 0 1 113 4 0.0 3 0 0 0 4 114 2 0.0 3 0 0 0 2 116 2 0.0 3 0 0 0 2 118 1 0.0 3 0 0 0 1 119 4 0.0 3 0 0 0 4 121 1 0.0 3 0 0 0 1 123 2 0.0 3 0 0 0 2 127 1 0.0 3 0 0 0 1 128 2 0.0 3 0 0 0 2 129 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 132 35 0.0 3 0 0 0 35 133 2 0.0 3 0 0 0 2 134 10 0.0 3 0 0 0 10 135 4 0.0 3 0 0 0 4 137 3 0.0 3 0 0 0 3 138 2 0.0 3 0 0 0 2 140 1 0.0 3 0 0 1 141 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 144 4 0.0 3 0 0 0 4 145 1 0.0 3 0 0 0 1 146 1 0.0 3 1 147 4 0.0 3 2 0 0 2 149 2 0.0 3 0 0 0 2 150 3 0.0 3 0 0 0 3 151 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 159 4 0.0 3 0 0 0 4 160 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 166 2 0.0 3 0 0 0 2 170 2 0.0 3 0 0 0 2 172 1 0.0 3 0 0 0 1 177 2 0.0 3 1 0 0 1 178 1 0.0 3 0 0 1 179 4 0.0 3 0 0 1 3 181 1 0.0 3 0 1 183 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 1 202 1 0.0 3 0 0 0 1 204 2 0.0 3 0 0 0 2 205 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 214 3 0.0 3 0 0 1 2 217 1 0.0 3 0 0 0 1 218 2 0.0 3 0 0 0 2 221 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.24 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 6,866 Reads with adapters: 439 (6.4%) Reads written (passing filters): 6,866 (100.0%) Total basepairs processed: 1,839,466 bp Total written (filtered): 1,838,133 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 439 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 55.8% C: 18.5% G: 22.8% T: 3.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 428 107.3 0 428 4 10 26.8 0 10 9 1 0.0 0 0 1