This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCTGTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.71 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 61,571 (2.3%) Reads that were too short: 11,036 (0.4%) Reads written (passing filters): 50,535 (1.9%) Total basepairs processed: 800,111,879 bp Total written (filtered): 9,596,034 bp (1.2%) === Adapter 1 === Sequence: CCTGTC; Type: regular 5'; Length: 6; Trimmed: 61571 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 269 649.0 0 269 7 6702 649.0 0 6702 8 309 649.0 0 309 9 5261 649.0 0 5261 10 29 649.0 0 29 11 30 649.0 0 30 12 32 649.0 0 32 13 26 649.0 0 26 14 29 649.0 0 29 15 19 649.0 0 19 16 31 649.0 0 31 17 35 649.0 0 35 18 36 649.0 0 36 19 40 649.0 0 40 20 28 649.0 0 28 21 32 649.0 0 32 22 35 649.0 0 35 23 38 649.0 0 38 24 21 649.0 0 21 25 24 649.0 0 24 26 29 649.0 0 29 27 30 649.0 0 30 28 34 649.0 0 34 29 31 649.0 0 31 30 222 649.0 0 222 31 54 649.0 0 54 32 54 649.0 0 54 33 434 649.0 0 434 34 325 649.0 0 325 35 261 649.0 0 261 36 43 649.0 0 43 37 21 649.0 0 21 38 35 649.0 0 35 39 42 649.0 0 42 40 38 649.0 0 38 41 32 649.0 0 32 42 33 649.0 0 33 43 48 649.0 0 48 44 42 649.0 0 42 45 62 649.0 0 62 46 95 649.0 0 95 47 68 649.0 0 68 48 51 649.0 0 51 49 47 649.0 0 47 50 54 649.0 0 54 51 37 649.0 0 37 52 37 649.0 0 37 53 45 649.0 0 45 54 30 649.0 0 30 55 35 649.0 0 35 56 66 649.0 0 66 57 116 649.0 0 116 58 74 649.0 0 74 59 93 649.0 0 93 60 37 649.0 0 37 61 39 649.0 0 39 62 40 649.0 0 40 63 45 649.0 0 45 64 71 649.0 0 71 65 164 649.0 0 164 66 61 649.0 0 61 67 43 649.0 0 43 68 42 649.0 0 42 69 32 649.0 0 32 70 47 649.0 0 47 71 36 649.0 0 36 72 277 649.0 0 277 73 117 649.0 0 117 74 127 649.0 0 127 75 41 649.0 0 41 76 47 649.0 0 47 77 49 649.0 0 49 78 41 649.0 0 41 79 48 649.0 0 48 80 38 649.0 0 38 81 37 649.0 0 37 82 39 649.0 0 39 83 46 649.0 0 46 84 98 649.0 0 98 85 172 649.0 0 172 86 803 649.0 0 803 87 342 649.0 0 342 88 45 649.0 0 45 89 55 649.0 0 55 90 64 649.0 0 64 91 55 649.0 0 55 92 29 649.0 0 29 93 39 649.0 0 39 94 87 649.0 0 87 95 42 649.0 0 42 96 57 649.0 0 57 97 45 649.0 0 45 98 48 649.0 0 48 99 58 649.0 0 58 100 36 649.0 0 36 101 43 649.0 0 43 102 60 649.0 0 60 103 37 649.0 0 37 104 101 649.0 0 101 105 107 649.0 0 107 106 100 649.0 0 100 107 812 649.0 0 812 108 748 649.0 0 748 109 1419 649.0 0 1419 110 40 649.0 0 40 111 35 649.0 0 35 112 42 649.0 0 42 113 138 649.0 0 138 114 35 649.0 0 35 115 36 649.0 0 36 116 31 649.0 0 31 117 40 649.0 0 40 118 28 649.0 0 28 119 72 649.0 0 72 120 34 649.0 0 34 121 62 649.0 0 62 122 70 649.0 0 70 123 182 649.0 0 182 124 49 649.0 0 49 125 45 649.0 0 45 126 74 649.0 0 74 127 537 649.0 0 537 128 476 649.0 0 476 129 760 649.0 0 760 130 345 649.0 0 345 131 52 649.0 0 52 132 89 649.0 0 89 133 51 649.0 0 51 134 51 649.0 0 51 135 62 649.0 0 62 136 99 649.0 0 99 137 72 649.0 0 72 138 70 649.0 0 70 139 52 649.0 0 52 140 95 649.0 0 95 141 104 649.0 0 104 142 113 649.0 0 113 143 623 649.0 0 623 144 295 649.0 0 295 145 783 649.0 0 783 146 70 649.0 0 70 147 164 649.0 0 164 148 97 649.0 0 97 149 108 649.0 0 108 150 55 649.0 0 55 151 80 649.0 0 80 152 306 649.0 0 306 153 796 649.0 0 796 154 351 649.0 0 351 155 336 649.0 0 336 156 181 649.0 0 181 157 86 649.0 0 86 158 71 649.0 0 71 159 118 649.0 0 118 160 134 649.0 0 134 161 124 649.0 0 124 162 77 649.0 0 77 163 125 649.0 0 125 164 49 649.0 0 49 165 58 649.0 0 58 166 64 649.0 0 64 167 57 649.0 0 57 168 62 649.0 0 62 169 230 649.0 0 230 170 94 649.0 0 94 171 105 649.0 0 105 172 83 649.0 0 83 173 92 649.0 0 92 174 209 649.0 0 209 175 141 649.0 0 141 176 123 649.0 0 123 177 70 649.0 0 70 178 67 649.0 0 67 179 99 649.0 0 99 180 118 649.0 0 118 181 117 649.0 0 117 182 1126 649.0 0 1126 183 318 649.0 0 318 184 304 649.0 0 304 185 365 649.0 0 365 186 4386 649.0 0 4386 187 6725 649.0 0 6725 188 2514 649.0 0 2514 189 631 649.0 0 631 190 40 649.0 0 40 191 44 649.0 0 44 192 25 649.0 0 25 193 30 649.0 0 30 194 55 649.0 0 55 195 35 649.0 0 35 196 54 649.0 0 54 197 45 649.0 0 45 198 33 649.0 0 33 199 42 649.0 0 42 200 42 649.0 0 42 201 52 649.0 0 52 202 68 649.0 0 68 203 55 649.0 0 55 204 87 649.0 0 87 205 63 649.0 0 63 206 60 649.0 0 60 207 53 649.0 0 53 208 79 649.0 0 79 209 55 649.0 0 55 210 45 649.0 0 45 211 44 649.0 0 44 212 57 649.0 0 57 213 91 649.0 0 91 214 37 649.0 0 37 215 62 649.0 0 62 216 66 649.0 0 66 217 260 649.0 0 260 218 531 649.0 0 531 219 372 649.0 0 372 220 40 649.0 0 40 221 62 649.0 0 62 222 178 649.0 0 178 223 143 649.0 0 143 224 71 649.0 0 71 225 85 649.0 0 85 226 79 649.0 0 79 227 97 649.0 0 97 228 46 649.0 0 46 229 49 649.0 0 49 230 52 649.0 0 52 231 51 649.0 0 51 232 38 649.0 0 38 233 45 649.0 0 45 234 65 649.0 0 65 235 54 649.0 0 54 236 56 649.0 0 56 237 61 649.0 0 61 238 66 649.0 0 66 239 47 649.0 0 47 240 45 649.0 0 45 241 69 649.0 0 69 242 118 649.0 0 118 243 131 649.0 0 131 244 80 649.0 0 80 245 74 649.0 0 74 246 69 649.0 0 69 247 54 649.0 0 54 248 54 649.0 0 54 249 54 649.0 0 54 250 225 649.0 0 225 251 110 649.0 0 110 252 231 649.0 0 231 253 70 649.0 0 70 254 92 649.0 0 92 255 61 649.0 0 61 256 63 649.0 0 63 257 70 649.0 0 70 258 65 649.0 0 65 259 67 649.0 0 67 260 102 649.0 0 102 261 215 649.0 0 215 262 287 649.0 0 287 263 101 649.0 0 101 264 161 649.0 0 161 265 48 649.0 0 48 266 47 649.0 0 47 267 67 649.0 0 67 268 51 649.0 0 51 269 83 649.0 0 83 270 86 649.0 0 86 271 110 649.0 0 110 272 143 649.0 0 143 273 99 649.0 0 99 274 65 649.0 0 65 275 61 649.0 0 61 276 60 649.0 0 60 277 63 649.0 0 63 278 115 649.0 0 115 279 143 649.0 0 143 280 396 649.0 0 396 281 265 649.0 0 265 282 347 649.0 0 347 283 57 649.0 0 57 284 53 649.0 0 53 285 88 649.0 0 88 286 81 649.0 0 81 287 62 649.0 0 62 288 131 649.0 0 131 289 134 649.0 0 134 290 119 649.0 0 119 291 169 649.0 0 169 292 82 649.0 0 82 293 66 649.0 0 66 294 112 649.0 0 112 295 115 649.0 0 115 296 212 649.0 0 212 297 422 649.0 0 422 298 204 649.0 0 204 299 273 649.0 0 273 300 138 649.0 0 138 301 131 649.0 0 131 Finished in 2.13 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 50,535 Reads with adapters: 7,041 (13.9%) Reads that were too short: 32 (0.1%) Reads written (passing filters): 7,009 (13.9%) Total basepairs processed: 9,596,034 bp Total written (filtered): 1,897,304 bp (19.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 7041 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 8 0.0 3 0 0 8 19 85 0.0 3 0 82 3 20 6667 0.0 4 6525 142 21 26 0.0 4 2 23 0 0 1 22 5 0.0 4 2 0 3 23 5 0.0 4 0 0 0 5 25 2 0.0 4 0 0 0 0 2 33 1 0.0 4 1 53 1 0.0 4 0 0 0 0 1 61 1 0.0 4 1 62 1 0.0 4 0 0 0 0 1 63 1 0.0 4 1 64 1 0.0 4 0 1 67 1 0.0 4 0 1 106 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 109 1 0.0 4 1 118 1 0.0 4 0 0 0 0 1 126 4 0.0 4 0 0 0 0 4 127 203 0.0 4 0 0 0 0 203 144 1 0.0 4 0 1 148 2 0.0 4 1 1 149 1 0.0 4 0 0 0 0 1 150 2 0.0 4 1 1 151 1 0.0 4 1 153 1 0.0 4 0 0 0 1 154 1 0.0 4 0 1 157 2 0.0 4 0 0 0 0 2 158 1 0.0 4 0 0 0 0 1 160 1 0.0 4 0 0 0 0 1 185 1 0.0 4 0 1 228 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 231 1 0.0 4 0 0 0 0 1 238 8 0.0 4 0 0 0 0 8 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.24 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 7,009 Reads with adapters: 170 (2.4%) Reads written (passing filters): 7,009 (100.0%) Total basepairs processed: 1,897,304 bp Total written (filtered): 1,895,297 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 170 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.7% C: 4.1% G: 90.6% T: 0.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 9 109.5 0 9 4 1 27.4 0 1 10 3 0.0 1 0 3 11 151 0.0 1 1 150 12 5 0.0 1 0 5 225 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.